Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 17 | 1 | 1 | 6 | 2 | 18 | 12 | 18 | 22 | 9 | 17 | 16 | 11 | 28 | 17 | 13 | 20 | 3 | 6 | 5 | 1 | 1 | 6 |
Bud | 8 | 2 | 17 | 5 | 16 | 23 | 63 | 57 | 68 | 64 | 72 | 63 | 3 | 7 | 4 | 2 | 3 | 4 | 2 | 1 | 1 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 2 | 7 | 9 | 5 | 6 | 8 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 8 | 8 | 11 | 19 | 16 | 13 | 25 | 14 | 19 | 15 | 24 | 19 | 32 | 50 | 24 | 16 | 22 | 1 | 1 | 0 | 4 | 5 | 7 |
Endoplasmic Reticulum | 4 | 15 | 0 | 1 | 0 | 1 | 2 | 4 | 1 | 8 | 3 | 13 | 3 | 3 | 8 | 4 | 8 | 3 | 0 | 0 | 0 | 1 | 0 | 1 |
Endosome | 352 | 127 | 326 | 116 | 201 | 169 | 150 | 197 | 194 | 173 | 117 | 120 | 287 | 433 | 384 | 74 | 94 | 51 | 445 | 206 | 357 | 328 | 405 | 422 |
Golgi | 6 | 15 | 4 | 2 | 3 | 0 | 0 | 2 | 4 | 7 | 1 | 3 | 3 | 9 | 6 | 25 | 24 | 27 | 12 | 10 | 13 | 7 | 14 | 27 |
Mitochondria | 83 | 36 | 21 | 70 | 72 | 147 | 177 | 138 | 188 | 268 | 303 | 266 | 4 | 0 | 0 | 5 | 15 | 14 | 3 | 5 | 2 | 8 | 5 | 3 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 8 | 3 | 2 | 5 | 4 | 1 | 3 | 0 | 3 | 2 | 3 | 3 | 4 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 4 |
Nucleolus | 66 | 18 | 38 | 32 | 47 | 35 | 53 | 43 | 37 | 39 | 41 | 34 | 11 | 11 | 11 | 4 | 7 | 9 | 1 | 0 | 0 | 2 | 3 | 3 |
Peroxisomes | 6 | 0 | 97 | 83 | 77 | 77 | 101 | 97 | 67 | 98 | 63 | 66 | 30 | 14 | 13 | 0 | 3 | 0 | 9 | 4 | 28 | 4 | 12 | 6 |
SpindlePole | 52 | 15 | 132 | 61 | 88 | 61 | 113 | 108 | 117 | 105 | 84 | 74 | 52 | 112 | 80 | 18 | 24 | 14 | 21 | 6 | 32 | 12 | 7 | 3 |
Vac/Vac Membrane | 70 | 47 | 14 | 2 | 15 | 8 | 7 | 7 | 13 | 19 | 12 | 19 | 39 | 90 | 90 | 28 | 41 | 15 | 3 | 5 | 2 | 12 | 10 | 10 |
Unique Cell Count | 460 | 220 | 429 | 259 | 348 | 391 | 478 | 492 | 502 | 610 | 530 | 528 | 341 | 527 | 510 | 145 | 171 | 130 | 508 | 255 | 449 | 387 | 474 | 504 |
Labelled Cell Count | 679 | 303 | 660 | 389 | 550 | 544 | 708 | 700 | 733 | 833 | 733 | 710 | 471 | 724 | 677 | 204 | 248 | 181 | 508 | 255 | 449 | 387 | 474 | 504 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 9.0 | 7.9 | 7.7 | 7.9 | 6.9 | 6.7 | 6.8 | 6.8 | 6.8 | 6.3 | 6.6 | 9.7 | 10.0 | 9.6 | 13.8 | 13.5 | 12.7 | 9.4 | 10.0 | 11.1 |
Std Deviation (1e-4) | 1.1 | 1.5 | 1.2 | 1.6 | 1.8 | 1.2 | 1.1 | 1.3 | 1.2 | 1.3 | 1.1 | 1.3 | 1.4 | 1.3 | 1.2 | 3.4 | 2.6 | 3.4 | 1.6 | 1.5 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.01 | -0.19 | -0.23 | -0.22 | -0.23 | -0.21 | -0.32 | -0.26 | 0.29 | 0.34 | 0.27 | 0.8 | 0.78 | 0.69 | 0.26 | 0.34 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.7 | 2.8 | 3.6 | 3.6 | 0 | 3.4 | 4.2 | 2.6 | 4.6 | 0 | 0 | 0 |
Bud | -1.5 | 0.4 | 1.3 | 4.9 | 4.2 | 5.1 | 3.9 | 5.1 | 4.4 | -2.7 | -2.6 | -3.3 | 0 | -1.4 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.8 | 2.7 | 1.9 | 0.9 | 2.6 | 0.9 | 1.2 | 1.0 | 2.3 | 2.8 | 3.2 | 5.0 | 6.7 | 4.2 | 6.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -8.3 | -5.4 | -9.6 | -13.4 | -11.0 | -11.4 | -15.1 | -16.7 | -16.4 | 2.8 | 2.3 | -0.2 | -5.7 | -5.1 | -7.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0 | 7.8 | 6.9 | 8.7 |
Mitochondria | 8.3 | 6.7 | 11.6 | 11.7 | 9.3 | 11.9 | 13.8 | 17.0 | 15.3 | -2.9 | -5.1 | -5.1 | -0.7 | 1.8 | 2.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.5 | 2.1 | 0 | 1.1 | -0.1 | -0.8 | -1.5 | -0.6 | -1.4 | -3.2 | -4.7 | -4.6 | -2.4 | -2.0 | -0.7 |
Peroxisomes | 2.7 | -0.2 | -1.0 | -0.5 | -1.1 | -3.7 | -2.7 | -4.4 | -4.1 | -5.1 | -9.6 | -9.5 | -6.3 | -6.2 | -6.0 |
SpindlePole | -2.0 | -1.7 | -5.1 | -2.4 | -3.0 | -2.6 | -5.1 | -5.5 | -6.3 | -5.0 | -3.4 | -5.5 | -4.3 | -4.2 | -4.5 |
Vacuole | -2.1 | 0.8 | -1.1 | -1.8 | -1.9 | -0.6 | -0.1 | -0.9 | 0.3 | 4.5 | 6.8 | 7.0 | 6.4 | 7.9 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.579 | 6.5784 | 6.4226 | 5.8247 | 5.5563 | 6.5745 | 5.5385 | 6.3925 | 6.0945 | 5.7465 | 5.5333 | 6.288 | 5.2319 | 6.5389 | 5.8215 | 5.8429 | 5.5509 | 5.7975 |
Actin | 0.0069 | 0.0003 | 0.0009 | 0 | 0.0008 | 0.0005 | 0.0123 | 0.001 | 0.0066 | 0 | 0.0008 | 0.0006 | 0.0127 | 0.0005 | 0.0009 | 0.0014 | 0 | 0.0002 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 |
Bud Neck | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0012 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 |
Bud Periphery | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 |
Bud Site | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0014 | 0.0016 | 0.0043 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0027 | 0.0001 | 0.0001 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0073 | 0.0096 | 0.0082 | 0.0017 | 0.0023 | 0.0078 | 0.0059 | 0.0111 | 0.0055 | 0.0019 | 0.004 | 0.0017 | 0.0037 | 0.0048 | 0.0027 | 0.004 | 0.002 | 0.0031 |
Cytoplasmic Foci | 0.2321 | 0.2351 | 0.2392 | 0.1906 | 0.2539 | 0.2347 | 0.2062 | 0.2651 | 0.222 | 0.2305 | 0.2052 | 0.2194 | 0.212 | 0.249 | 0.2491 | 0.1196 | 0.2721 | 0.2155 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0009 | 0.0007 | 0.0008 | 0.0002 | 0.0001 | 0.0005 | 0.0027 | 0.0009 | 0.0007 | 0.0001 | 0.0028 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0009 | 0.0004 | 0.0003 |
Endosome | 0.5293 | 0.5662 | 0.5422 | 0.6671 | 0.5559 | 0.4748 | 0.588 | 0.5699 | 0.5437 | 0.6611 | 0.5861 | 0.5179 | 0.5689 | 0.566 | 0.5541 | 0.7191 | 0.5767 | 0.5418 |
Golgi | 0.0823 | 0.0874 | 0.1312 | 0.1245 | 0.114 | 0.1748 | 0.0962 | 0.092 | 0.1233 | 0.0734 | 0.1041 | 0.1758 | 0.1069 | 0.1155 | 0.138 | 0.1122 | 0.0986 | 0.174 |
Lipid Particles | 0.0619 | 0.0789 | 0.0289 | 0.0069 | 0.0234 | 0.0451 | 0.0493 | 0.0176 | 0.0379 | 0.0175 | 0.0353 | 0.0377 | 0.0515 | 0.0397 | 0.0235 | 0.0181 | 0.0215 | 0.032 |
Mitochondria | 0.0013 | 0.0006 | 0.0012 | 0.0004 | 0.0005 | 0.0025 | 0.0039 | 0.0007 | 0.0172 | 0.0003 | 0.002 | 0.0028 | 0.0017 | 0.0003 | 0.0006 | 0.0019 | 0.0002 | 0.0009 |
None | 0.0031 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0.0015 | 0.0011 | 0.0003 | 0 | 0.0001 | 0 | 0.0041 | 0 | 0 | 0.0006 | 0.0001 | 0 |
Nuclear Periphery | 0.0023 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0062 | 0.0005 | 0.0004 | 0.0001 | 0.0154 | 0.0006 | 0.0006 | 0.0004 | 0.0001 | 0.0017 | 0.004 | 0.0002 |
Nucleolus | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0003 | 0 | 0 | 0.0004 | 0 | 0 |
Nucleus | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0017 | 0.0002 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0004 | 0 | 0 | 0.0015 | 0.0001 | 0 |
Peroxisomes | 0.0602 | 0.0142 | 0.0377 | 0.005 | 0.046 | 0.046 | 0.0146 | 0.0239 | 0.0253 | 0.0117 | 0.0223 | 0.0363 | 0.0297 | 0.0144 | 0.0247 | 0.0037 | 0.0193 | 0.025 |
Punctate Nuclear | 0.0062 | 0.0016 | 0.0007 | 0.0001 | 0.0003 | 0.0005 | 0.0029 | 0.0007 | 0.0004 | 0.0003 | 0.0011 | 0.0006 | 0.0012 | 0.0003 | 0.0003 | 0.0027 | 0.001 | 0.0002 |
Vacuole | 0.0035 | 0.0032 | 0.0052 | 0.0018 | 0.001 | 0.0093 | 0.0039 | 0.0107 | 0.007 | 0.0018 | 0.0093 | 0.0026 | 0.0028 | 0.0057 | 0.0037 | 0.0057 | 0.0021 | 0.0042 |
Vacuole Periphery | 0.0017 | 0.0013 | 0.0021 | 0.0012 | 0.0012 | 0.0024 | 0.0024 | 0.0022 | 0.0026 | 0.0011 | 0.0107 | 0.0015 | 0.0013 | 0.0028 | 0.0017 | 0.0028 | 0.0017 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.8623 | 24.6775 | 12.3874 | 22.6492 | 14.7377 | 25.9672 | 27.8869 | 23.0319 | 24.8206 | 24.8636 |
Translational Efficiency | 1.732 | 1.4892 | 2.3913 | 1.3949 | 2.0602 | 1.5916 | 1.3381 | 1.7326 | 1.4695 | 1.3893 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
680 | 449 | 256 | 1422 | 2359 | 1827 | 155 | 1018 | 3039 | 2276 | 411 | 2440 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 836.84 | 1040.60 | 1180.73 | 1209.73 | 923.88 | 1019.43 | 1318.87 | 1267.65 | 904.40 | 1023.61 | 1232.83 | 1233.89 |
Standard Deviation | 116.72 | 149.24 | 164.17 | 186.17 | 123.79 | 136.72 | 166.24 | 218.51 | 127.51 | 139.53 | 178.02 | 202.32 |
Intensity Change Log 2 | — | 0.314392 | 0.496655 | 0.531661 | — | 0.141985 | 0.513525 | 0.456379 | — | 0.226500 | 0.505532 | 0.492650 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002010 | 0.002081 | 0.002157 | 0.024149 | 0.000286 | 0.000852 | 0.001075 | 0.049452 | 0.000672 | 0.001095 | 0.001749 | 0.034706 |
Bud Neck | 0.002305 | 0.001333 | 0.008099 | 0.002100 | 0.001044 | 0.001071 | 0.006951 | 0.004275 | 0.001326 | 0.001123 | 0.007666 | 0.003008 |
Bud Site | 0.005684 | 0.012932 | 0.015201 | 0.022468 | 0.001813 | 0.004980 | 0.012083 | 0.049117 | 0.002679 | 0.006548 | 0.014025 | 0.033586 |
Cell Periphery | 0.000149 | 0.000054 | 0.000101 | 0.000183 | 0.000029 | 0.000061 | 0.000053 | 0.000341 | 0.000056 | 0.000060 | 0.000083 | 0.000249 |
Cytoplasm | 0.002973 | 0.010866 | 0.002587 | 0.009809 | 0.001466 | 0.002671 | 0.001901 | 0.012487 | 0.001803 | 0.004288 | 0.002328 | 0.010926 |
Cytoplasmic Foci | 0.219366* | 0.193721* | 0.205158* | 0.151740 | 0.113158 | 0.150144 | 0.246323* | 0.195681* | 0.136923 | 0.158741 | 0.220683* | 0.170073 |
Eisosomes | 0.000041 | 0.000017 | 0.000028 | 0.000084 | 0.000010 | 0.000013 | 0.000014 | 0.000114 | 0.000017 | 0.000014 | 0.000023 | 0.000096 |
Endoplasmic Reticulum | 0.000181 | 0.000089 | 0.000263 | 0.000432 | 0.000087 | 0.000111 | 0.000269 | 0.000453 | 0.000108 | 0.000106 | 0.000265 | 0.000441 |
Endosome | 0.524332* | 0.598805* | 0.521984* | 0.379449* | 0.740212* | 0.683986* | 0.470694* | 0.339898* | 0.691907* | 0.667182* | 0.502641* | 0.362948* |
Golgi | 0.148928* | 0.120650* | 0.062574 | 0.289447* | 0.085492 | 0.087773 | 0.047945 | 0.215752* | 0.099686 | 0.094259 | 0.057057 | 0.258700* |
Lipid Particles | 0.020302 | 0.007437 | 0.012679 | 0.008210 | 0.015100 | 0.012407 | 0.015175 | 0.010803 | 0.016264 | 0.011427 | 0.013621 | 0.009292 |
Mitochondria | 0.004120 | 0.002752 | 0.001141 | 0.002696 | 0.002884 | 0.004050 | 0.000833 | 0.004607 | 0.003161 | 0.003794 | 0.001025 | 0.003494 |
Mitotic Spindle | 0.002378 | 0.004830 | 0.019701 | 0.010248 | 0.000875 | 0.005637 | 0.033470 | 0.014269 | 0.001211 | 0.005478 | 0.024894 | 0.011925 |
None | 0.000151 | 0.000018 | 0.000014 | 0.000041 | 0.000088 | 0.000020 | 0.000007 | 0.000223 | 0.000102 | 0.000020 | 0.000012 | 0.000117 |
Nuclear Periphery | 0.000048 | 0.000032 | 0.000677 | 0.000197 | 0.000034 | 0.000067 | 0.000862 | 0.000134 | 0.000037 | 0.000060 | 0.000747 | 0.000171 |
Nuclear Periphery Foci | 0.000761 | 0.001037 | 0.011285 | 0.002140 | 0.001165 | 0.001763 | 0.017963 | 0.001175 | 0.001075 | 0.001620 | 0.013804 | 0.001737 |
Nucleolus | 0.000772 | 0.000020 | 0.000600 | 0.000020 | 0.000280 | 0.000089 | 0.000239 | 0.000055 | 0.000390 | 0.000076 | 0.000464 | 0.000035 |
Nucleus | 0.001600 | 0.000016 | 0.000323 | 0.000057 | 0.000064 | 0.000039 | 0.000147 | 0.000085 | 0.000408 | 0.000035 | 0.000257 | 0.000069 |
Peroxisomes | 0.055729 | 0.024085 | 0.052589 | 0.015606 | 0.021240 | 0.015198 | 0.034045 | 0.038611 | 0.028958 | 0.016951 | 0.045595 | 0.025204 |
Vacuole | 0.005688 | 0.014124 | 0.072392 | 0.064747 | 0.010917 | 0.021795 | 0.098549 | 0.052009 | 0.009747 | 0.020282 | 0.082257 | 0.059433 |
Vacuole Periphery | 0.002481 | 0.005101 | 0.010445 | 0.016174 | 0.003755 | 0.007271 | 0.011401 | 0.010460 | 0.003470 | 0.006843 | 0.010806 | 0.013790 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.09 | -0.17 | -6.92 | -6.85 | -6.76 | -1.66 | -0.88 | -9.77 | -9.63 | -9.47 | -1.29 | -1.76 | -12.14 | -11.93 | -11.50 |
Bud Neck | 1.03 | -3.81 | 0.17 | -1.59 | 4.50 | -0.13 | -2.41 | -7.55 | -8.05 | 1.05 | 0.68 | -5.08 | -5.37 | -7.55 | 3.70 |
Bud Site | -2.08 | -3.92 | -7.61 | -2.65 | -2.61 | -4.05 | -4.12 | -12.30 | -11.34 | -7.99 | -4.11 | -6.86 | -15.60 | -12.74 | -7.68 |
Cell Periphery | 1.76 | 0.84 | -0.63 | -5.76 | -2.88 | -1.98 | -2.12 | -3.21 | -2.85 | -2.95 | -0.23 | -1.53 | -4.38 | -4.27 | -3.76 |
Cytoplasm | -2.64 | 0.33 | -5.02 | 0.31 | -5.12 | -1.78 | -1.37 | -7.97 | -6.73 | -7.43 | -3.09 | -1.29 | -9.57 | -5.70 | -8.45 |
Cytoplasmic Foci | 2.43 | 1.09 | 8.60 | 4.71 | 4.68 | -7.74 | -7.95 | -13.19 | -6.78 | 2.85 | -4.89 | -8.71 | -7.76 | -2.44 | 5.16 |
Eisosomes | 3.08 | 1.60 | -1.21 | -1.97 | -1.64 | -1.71 | -1.46 | -6.40 | -6.15 | -6.00 | 1.27 | -1.87 | -3.78 | -3.93 | -3.49 |
Endoplasmic Reticulum | 1.66 | -1.16 | -3.16 | -5.69 | -1.92 | -0.85 | -2.36 | -4.62 | -4.43 | -1.41 | 0.07 | -3.25 | -6.48 | -6.95 | -2.38 |
Endosome | -4.68 | 0.26 | 12.20 | 16.03 | 8.54 | 7.37 | 13.68 | 44.46* | 35.95 | 6.15 | 3.53 | 14.91 | 48.59* | 42.12 | 10.60 |
Golgi | 3.13 | 10.24 | -15.56 | -17.71 | -25.36* | -0.60 | 6.25 | -16.91 | -16.45 | -18.32* | 1.55 | 8.79 | -28.66 | -29.25 | -30.69* |
Lipid Particles | 3.79 | 1.92 | 3.81 | -0.50 | 1.58 | 1.34 | -0.01 | 2.18 | 0.77 | 0.82 | 2.78 | 0.94 | 4.61 | 1.43 | 1.62 |
Mitochondria | 0.96 | 3.19 | 1.49 | 0.05 | -3.50 | -1.46 | 2.73 | -1.48 | -0.42 | -3.03 | -0.88 | 4.58 | -0.48 | 0.41 | -4.32 |
Mitotic Spindle | -1.23 | -3.54 | -4.44 | -2.47 | 1.88 | -5.38 | -4.00 | -7.40 | -4.45 | 2.34 | -5.10 | -5.54 | -9.89 | -5.13 | 2.95 |
None | 2.29 | 2.34 | 1.83 | -1.17 | -1.35 | 0.84 | 0.97 | -0.62 | -1.05 | -1.11 | 1.31 | 1.41 | -0.09 | -1.20 | -1.29 |
Nuclear Periphery | 0.55 | -4.30 | -4.03 | -5.84 | 3.25 | -3.27 | -3.73 | -8.08 | -4.53 | 3.29 | -2.35 | -5.77 | -7.54 | -6.10 | 4.65 |
Nuclear Periphery Foci | -0.88 | -3.10 | -3.19 | -1.37 | 2.69 | -0.97 | -2.59 | -0.31 | 0.84 | 2.57 | -1.17 | -3.95 | -1.94 | -0.32 | 3.72 |
Nucleolus | 1.68 | 0.67 | 1.68 | 0.15 | 1.52 | 0.73 | -0.01 | 0.88 | 0.92 | 2.70 | 1.55 | -0.04 | 1.74 | 1.48 | 1.87 |
Nucleus | 1.46 | 1.20 | 1.43 | -1.53 | 2.00 | 0.64 | -1.38 | -0.60 | -1.46 | 1.14 | 1.51 | 0.56 | 1.38 | -1.63 | 2.29 |
Peroxisomes | 5.70 | 0.45 | 8.29 | 2.26 | 5.47 | 3.22 | -2.49 | -4.96 | -6.88 | -0.64 | 6.30 | -3.51 | 1.72 | -3.97 | 4.28 |
Vacuole | -3.83 | -9.86 | -19.39 | -14.38 | 1.03 | -6.61 | -7.90 | -13.05 | -9.12 | 4.02 | -7.62 | -12.17 | -22.57 | -16.28 | 3.60 |
Vacuole Periphery | -2.17 | -4.22 | -11.96 | -7.32 | -2.76 | -4.29 | -3.77 | -6.27 | -2.61 | 0.43 | -4.84 | -5.23 | -12.74 | -7.30 | -1.96 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|