Standard name | |
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Human Ortholog | |
Description | Subunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 17 | 1 | 1 | 6 | 2 | 18 | 12 | 18 | 22 | 9 | 17 | 16 | 11 | 28 | 17 | 13 | 20 | 3 | 6 | 5 | 1 | 1 | 6 |
Bud | 8 | 2 | 17 | 5 | 16 | 23 | 63 | 57 | 68 | 64 | 72 | 63 | 3 | 7 | 4 | 2 | 3 | 4 | 2 | 1 | 1 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 2 | 7 | 9 | 5 | 6 | 8 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 8 | 8 | 11 | 19 | 16 | 13 | 25 | 14 | 19 | 15 | 24 | 19 | 32 | 50 | 24 | 16 | 22 | 1 | 1 | 0 | 4 | 5 | 7 |
Endoplasmic Reticulum | 4 | 15 | 0 | 1 | 0 | 1 | 2 | 4 | 1 | 8 | 3 | 13 | 3 | 3 | 8 | 4 | 8 | 3 | 0 | 0 | 0 | 1 | 0 | 1 |
Endosome | 352 | 127 | 326 | 116 | 201 | 169 | 150 | 197 | 194 | 173 | 117 | 120 | 287 | 433 | 384 | 74 | 94 | 51 | 445 | 206 | 357 | 328 | 405 | 422 |
Golgi | 6 | 15 | 4 | 2 | 3 | 0 | 0 | 2 | 4 | 7 | 1 | 3 | 3 | 9 | 6 | 25 | 24 | 27 | 12 | 10 | 13 | 7 | 14 | 27 |
Mitochondria | 83 | 36 | 21 | 70 | 72 | 147 | 177 | 138 | 188 | 268 | 303 | 266 | 4 | 0 | 0 | 5 | 15 | 14 | 3 | 5 | 2 | 8 | 5 | 3 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 8 | 3 | 2 | 5 | 4 | 1 | 3 | 0 | 3 | 2 | 3 | 3 | 4 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 4 |
Nucleolus | 66 | 18 | 38 | 32 | 47 | 35 | 53 | 43 | 37 | 39 | 41 | 34 | 11 | 11 | 11 | 4 | 7 | 9 | 1 | 0 | 0 | 2 | 3 | 3 |
Peroxisomes | 6 | 0 | 97 | 83 | 77 | 77 | 101 | 97 | 67 | 98 | 63 | 66 | 30 | 14 | 13 | 0 | 3 | 0 | 9 | 4 | 28 | 4 | 12 | 6 |
SpindlePole | 52 | 15 | 132 | 61 | 88 | 61 | 113 | 108 | 117 | 105 | 84 | 74 | 52 | 112 | 80 | 18 | 24 | 14 | 21 | 6 | 32 | 12 | 7 | 3 |
Vac/Vac Membrane | 70 | 47 | 14 | 2 | 15 | 8 | 7 | 7 | 13 | 19 | 12 | 19 | 39 | 90 | 90 | 28 | 41 | 15 | 3 | 5 | 2 | 12 | 10 | 10 |
Unique Cell Count | 460 | 220 | 429 | 259 | 348 | 391 | 478 | 492 | 502 | 610 | 530 | 528 | 341 | 527 | 510 | 145 | 171 | 130 | 508 | 255 | 449 | 387 | 474 | 504 |
Labelled Cell Count | 679 | 303 | 660 | 389 | 550 | 544 | 708 | 700 | 733 | 833 | 733 | 710 | 471 | 724 | 677 | 204 | 248 | 181 | 508 | 255 | 449 | 387 | 474 | 504 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 9.0 | 7.9 | 7.7 | 7.9 | 6.9 | 6.7 | 6.8 | 6.8 | 6.8 | 6.3 | 6.6 | 9.7 | 10.0 | 9.6 | 13.8 | 13.5 | 12.7 | 9.4 | 10.0 | 11.1 |
Std Deviation (1e-4) | 1.1 | 1.5 | 1.2 | 1.6 | 1.8 | 1.2 | 1.1 | 1.3 | 1.2 | 1.3 | 1.1 | 1.3 | 1.4 | 1.3 | 1.2 | 3.4 | 2.6 | 3.4 | 1.6 | 1.5 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.01 | -0.19 | -0.23 | -0.22 | -0.23 | -0.21 | -0.32 | -0.26 | 0.29 | 0.34 | 0.27 | 0.8 | 0.78 | 0.69 | 0.26 | 0.34 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.7 | 2.8 | 3.6 | 3.6 | 0 | 3.4 | 4.2 | 2.6 | 4.6 | 0 | 0 | 0 |
Bud | -1.5 | 0.4 | 1.3 | 4.9 | 4.2 | 5.1 | 3.9 | 5.1 | 4.4 | -2.7 | -2.6 | -3.3 | 0 | -1.4 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.8 | 2.7 | 1.9 | 0.9 | 2.6 | 0.9 | 1.2 | 1.0 | 2.3 | 2.8 | 3.2 | 5.0 | 6.7 | 4.2 | 6.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -8.3 | -5.4 | -9.6 | -13.4 | -11.0 | -11.4 | -15.1 | -16.7 | -16.4 | 2.8 | 2.3 | -0.2 | -5.7 | -5.1 | -7.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0 | 7.8 | 6.9 | 8.7 |
Mitochondria | 8.3 | 6.7 | 11.6 | 11.7 | 9.3 | 11.9 | 13.8 | 17.0 | 15.3 | -2.9 | -5.1 | -5.1 | -0.7 | 1.8 | 2.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.5 | 2.1 | 0 | 1.1 | -0.1 | -0.8 | -1.5 | -0.6 | -1.4 | -3.2 | -4.7 | -4.6 | -2.4 | -2.0 | -0.7 |
Peroxisomes | 2.7 | -0.2 | -1.0 | -0.5 | -1.1 | -3.7 | -2.7 | -4.4 | -4.1 | -5.1 | -9.6 | -9.5 | -6.3 | -6.2 | -6.0 |
SpindlePole | -2.0 | -1.7 | -5.1 | -2.4 | -3.0 | -2.6 | -5.1 | -5.5 | -6.3 | -5.0 | -3.4 | -5.5 | -4.3 | -4.2 | -4.5 |
Vacuole | -2.1 | 0.8 | -1.1 | -1.8 | -1.9 | -0.6 | -0.1 | -0.9 | 0.3 | 4.5 | 6.8 | 7.0 | 6.4 | 7.9 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.579 | 6.5784 | 6.4226 | 5.8247 | 5.5563 | 6.5745 | 5.5385 | 6.3925 | 6.0945 | 5.7465 | 5.5333 | 6.288 | 5.2319 | 6.5389 | 5.8215 | 5.8429 | 5.5509 | 5.7975 |
Actin | 0.0069 | 0.0003 | 0.0009 | 0 | 0.0008 | 0.0005 | 0.0123 | 0.001 | 0.0066 | 0 | 0.0008 | 0.0006 | 0.0127 | 0.0005 | 0.0009 | 0.0014 | 0 | 0.0002 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 |
Bud Neck | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0012 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 |
Bud Periphery | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 |
Bud Site | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0014 | 0.0016 | 0.0043 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0027 | 0.0001 | 0.0001 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0073 | 0.0096 | 0.0082 | 0.0017 | 0.0023 | 0.0078 | 0.0059 | 0.0111 | 0.0055 | 0.0019 | 0.004 | 0.0017 | 0.0037 | 0.0048 | 0.0027 | 0.004 | 0.002 | 0.0031 |
Cytoplasmic Foci | 0.2321 | 0.2351 | 0.2392 | 0.1906 | 0.2539 | 0.2347 | 0.2062 | 0.2651 | 0.222 | 0.2305 | 0.2052 | 0.2194 | 0.212 | 0.249 | 0.2491 | 0.1196 | 0.2721 | 0.2155 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0009 | 0.0007 | 0.0008 | 0.0002 | 0.0001 | 0.0005 | 0.0027 | 0.0009 | 0.0007 | 0.0001 | 0.0028 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0009 | 0.0004 | 0.0003 |
Endosome | 0.5293 | 0.5662 | 0.5422 | 0.6671 | 0.5559 | 0.4748 | 0.588 | 0.5699 | 0.5437 | 0.6611 | 0.5861 | 0.5179 | 0.5689 | 0.566 | 0.5541 | 0.7191 | 0.5767 | 0.5418 |
Golgi | 0.0823 | 0.0874 | 0.1312 | 0.1245 | 0.114 | 0.1748 | 0.0962 | 0.092 | 0.1233 | 0.0734 | 0.1041 | 0.1758 | 0.1069 | 0.1155 | 0.138 | 0.1122 | 0.0986 | 0.174 |
Lipid Particles | 0.0619 | 0.0789 | 0.0289 | 0.0069 | 0.0234 | 0.0451 | 0.0493 | 0.0176 | 0.0379 | 0.0175 | 0.0353 | 0.0377 | 0.0515 | 0.0397 | 0.0235 | 0.0181 | 0.0215 | 0.032 |
Mitochondria | 0.0013 | 0.0006 | 0.0012 | 0.0004 | 0.0005 | 0.0025 | 0.0039 | 0.0007 | 0.0172 | 0.0003 | 0.002 | 0.0028 | 0.0017 | 0.0003 | 0.0006 | 0.0019 | 0.0002 | 0.0009 |
None | 0.0031 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0.0015 | 0.0011 | 0.0003 | 0 | 0.0001 | 0 | 0.0041 | 0 | 0 | 0.0006 | 0.0001 | 0 |
Nuclear Periphery | 0.0023 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0062 | 0.0005 | 0.0004 | 0.0001 | 0.0154 | 0.0006 | 0.0006 | 0.0004 | 0.0001 | 0.0017 | 0.004 | 0.0002 |
Nucleolus | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0003 | 0 | 0 | 0.0004 | 0 | 0 |
Nucleus | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0017 | 0.0002 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0004 | 0 | 0 | 0.0015 | 0.0001 | 0 |
Peroxisomes | 0.0602 | 0.0142 | 0.0377 | 0.005 | 0.046 | 0.046 | 0.0146 | 0.0239 | 0.0253 | 0.0117 | 0.0223 | 0.0363 | 0.0297 | 0.0144 | 0.0247 | 0.0037 | 0.0193 | 0.025 |
Punctate Nuclear | 0.0062 | 0.0016 | 0.0007 | 0.0001 | 0.0003 | 0.0005 | 0.0029 | 0.0007 | 0.0004 | 0.0003 | 0.0011 | 0.0006 | 0.0012 | 0.0003 | 0.0003 | 0.0027 | 0.001 | 0.0002 |
Vacuole | 0.0035 | 0.0032 | 0.0052 | 0.0018 | 0.001 | 0.0093 | 0.0039 | 0.0107 | 0.007 | 0.0018 | 0.0093 | 0.0026 | 0.0028 | 0.0057 | 0.0037 | 0.0057 | 0.0021 | 0.0042 |
Vacuole Periphery | 0.0017 | 0.0013 | 0.0021 | 0.0012 | 0.0012 | 0.0024 | 0.0024 | 0.0022 | 0.0026 | 0.0011 | 0.0107 | 0.0015 | 0.0013 | 0.0028 | 0.0017 | 0.0028 | 0.0017 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.8623 | 24.6775 | 12.3874 | 22.6492 | 14.7377 | 25.9672 | 27.8869 | 23.0319 | 24.8206 | 24.8636 |
Translational Efficiency | 1.732 | 1.4892 | 2.3913 | 1.3949 | 2.0602 | 1.5916 | 1.3381 | 1.7326 | 1.4695 | 1.3893 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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