ORF
Human Ortholog
Description Putative protein of unknown function; may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0.13 0.13 0.11 0 0 0 0.05 0.1 0.19 0.1 0.09 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.69 0.72 0.81 0.88 0.86 0.83 0.82 0.8 0.78 0.81 0.85 0.79 0.8 0.79 0.81 0.84 0.73 0.73 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.18 0.05 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.06 0.05 0 0.07 0.12 0.08 0.08 0.09 0.1
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.08 0.08 0.07 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.15 0.14 0.09 0.15 0.14 0.13 0.18 0 0 0 0.05 0 0 0.05 0.06 0.1 0.15 0.1 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.23 0.05 0.06 0.06 0.15 0.14 0.2 0.18 0.24 0.23 0.12 0.18 0 0 0 0.14 0.25 0.24 0.68 0.54 0.42 0.48 0.56 0.56
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 25 17 5 2 1 2 2 2 1 0 1 164 175 150 2 3 5 22 56 94 47 64 39
Bud 4 14 8 6 2 5 9 11 11 5 5 8 23 23 9 1 2 0 3 2 1 0 6 10
Bud Neck 0 0 1 1 1 0 0 1 0 0 0 0 24 22 30 2 0 0 6 23 15 7 13 12
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 7 6 0 2 0 0 1 0 0 0 0 51 51 44 1 1 2 0 5 6 3 4 4
Cytoplasm 233 344 312 272 302 253 338 382 268 405 267 379 1039 1039 1060 387 294 149 12 14 8 12 15 12
Endoplasmic Reticulum 19 86 19 12 3 2 2 1 2 1 6 1 59 65 53 27 20 8 31 70 38 40 69 77
Endosome 16 9 17 1 5 2 1 1 0 0 0 0 7 8 0 5 15 10 29 32 38 38 52 68
Golgi 1 1 0 1 1 0 0 0 0 0 0 0 1 1 0 0 2 1 7 13 11 7 12 33
Mitochondria 6 4 7 11 5 47 57 43 52 69 42 84 1 1 0 24 15 3 19 35 51 72 73 57
Nucleus 2 0 0 1 1 3 5 11 11 9 6 14 2 2 5 7 8 9 0 0 0 0 1 0
Nuclear Periphery 1 1 0 0 0 1 1 0 1 2 1 3 5 1 0 0 1 0 0 0 2 0 1 3
Nucleolus 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 3 1 4 3 0 0 0 0 0 1 4 3 2 0 4 1 1 1 9 11 3 3
SpindlePole 3 1 7 2 3 2 1 1 0 1 0 0 47 56 71 2 2 2 2 0 1 2 2 7
Vac/Vac Membrane 79 23 25 20 53 42 81 87 81 116 38 87 8 9 4 67 100 50 298 305 207 229 425 439
Unique Cell Count 339 477 386 308 350 306 412 480 342 502 315 477 1306 1317 1309 463 404 205 441 564 494 479 758 786
Labelled Cell Count 374 515 423 333 384 362 497 542 428 609 365 578 1435 1456 1428 525 467 240 441 564 494 479 758 786


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.8 4.7 4.5 4.8 3.9 3.9 4.4 3.9 3.9 3.9 3.8 6.4 6.4 6.5 5.1 6.0 6.3 6.3 6.2 6.7
Std Deviation (1e-4) 0.6 0.7 0.9 1.2 1.3 1.3 1.1 1.2 1.1 1.1 1.0 0.9 1.0 1.0 1.1 1.1 1.2 1.3 1.1 1.3 1.6
Intensity Change (Log2) – – – -0.07 0.0 -0.27 -0.28 -0.12 -0.27 -0.3 -0.29 -0.34 0.44 0.44 0.46 0.1 0.34 0.41 0.41 0.39 0.49

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 4.6 4.9 4.1 -3.9 -3.3 -1.2
Bud 0 0 0 0 0 0 0 0 0 -0.4 -0.4 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 2.3 2.1 2.6 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 2.2 2.2 1.8 0 0 0
Cytoplasm 2.7 2.0 0.6 0.4 -0.5 -0.8 -0.1 1.4 -0.5 -0.5 -0.8 0.1 1.0 -2.7 -2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 -0.3 0 -0.7 0.6 0 -0.6
Endosome 0 0 0 0 0 0 0 0 0 -5.6 -5.5 0 -3.0 -0.5 0.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 2.6 1.6 0
Nucleus 0 0 0 0 0 3.6 0 0 3.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1.8 2.2 3.0 0 0 0
Vacuole 0 3.8 3.2 5.5 5.1 6.6 6.7 2.6 5.1 -7.3 -7.2 -8.2 3.7 7.0 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.303 1.8794 1.8491 1.7673 1.6807 1.6233 3.3222 4.1897 3.7128 3.4109 3.3602 4.0818 4.6025 4.793 5.072 6.0699 4.736 4.7153
Actin 0.0126 0.0128 0.0068 0.0076 0.0025 0.0095 0.0141 0.0005 0.0112 0.0066 0.0027 0.0011 0.0043 0.0014 0.0106 0.003 0.0002 0.0023
Bud 0.0021 0.0051 0.0099 0.0033 0.0245 0.0013 0.0011 0.002 0.002 0.002 0.002 0.0007 0.0026 0.0038 0.0025 0.0049 0.0007 0.0007
Bud Neck 0.0036 0.0037 0.0045 0.0219 0.0024 0.0145 0.0025 0.0019 0.0046 0.0025 0.0086 0.0068 0.0014 0.002 0.003 0.0079 0.0029 0.0035
Bud Periphery 0.0032 0.0049 0.0085 0.0146 0.0175 0.0042 0.0027 0.0028 0.0033 0.0066 0.0037 0.0024 0.0126 0.0093 0.0102 0.0292 0.0013 0.0037
Bud Site 0.0218 0.0392 0.0197 0.0015 0.0065 0.0018 0.0175 0.0255 0.0129 0.0023 0.0077 0.0012 0.0043 0.012 0.0067 0.0021 0.0018 0.0008
Cell Periphery 0.0052 0.004 0.003 0.0023 0.0045 0.0024 0.0089 0.0034 0.0031 0.003 0.0031 0.0024 0.0079 0.0055 0.0068 0.0108 0.0015 0.0035
Cytoplasm 0.2655 0.2891 0.2783 0.2779 0.3248 0.3104 0.2711 0.3126 0.2517 0.2902 0.3336 0.2989 0.2409 0.331 0.2637 0.2257 0.2624 0.3499
Cytoplasmic Foci 0.0622 0.0853 0.0798 0.0749 0.1011 0.0976 0.0968 0.0585 0.0892 0.0998 0.0813 0.0908 0.0694 0.0351 0.0525 0.0227 0.048 0.0571
Eisosomes 0.0014 0.0012 0.0005 0.0001 0.0003 0.0002 0.0007 0.0002 0.0003 0.0004 0.0003 0.0001 0.0006 0.0002 0.0003 0.0002 0 0.0002
Endoplasmic Reticulum 0.0218 0.0175 0.0303 0.0161 0.0206 0.023 0.0241 0.0299 0.0251 0.0173 0.0139 0.0324 0.2365 0.1556 0.2228 0.3276 0.2035 0.1861
Endosome 0.1521 0.1811 0.2015 0.3446 0.1659 0.1726 0.1392 0.1765 0.195 0.2007 0.2368 0.1825 0.1651 0.0997 0.1364 0.1436 0.2104 0.1289
Golgi 0.0073 0.0202 0.0142 0.0281 0.0108 0.0108 0.0243 0.0094 0.0185 0.0241 0.0153 0.0073 0.0141 0.0067 0.011 0.0066 0.0045 0.006
Lipid Particles 0.021 0.0142 0.0139 0.0095 0.0124 0.0188 0.0301 0.0203 0.0151 0.0192 0.0086 0.024 0.0303 0.0119 0.0197 0.0099 0.0052 0.0094
Mitochondria 0.0202 0.0075 0.016 0.0066 0.0105 0.0101 0.0189 0.0051 0.0099 0.008 0.0152 0.0035 0.0084 0.003 0.0041 0.0031 0.0032 0.0038
None 0.2916 0.2242 0.2217 0.0622 0.201 0.1881 0.2684 0.254 0.2337 0.2217 0.1147 0.1836 0.1277 0.1858 0.16 0.1051 0.0855 0.1295
Nuclear Periphery 0.0047 0.0046 0.0051 0.0053 0.0037 0.0055 0.0029 0.0034 0.0052 0.0064 0.0024 0.0033 0.0062 0.0076 0.0083 0.0064 0.0044 0.0059
Nucleolus 0.0015 0.0033 0.0025 0.0006 0.0043 0.0016 0.0014 0.0017 0.0016 0.0012 0.0008 0.001 0.0006 0.0014 0.0009 0.0005 0.0007 0.0005
Nucleus 0.0057 0.0085 0.0054 0.0027 0.0067 0.006 0.0032 0.0054 0.0044 0.0032 0.0028 0.004 0.0021 0.0073 0.0036 0.0026 0.0056 0.003
Peroxisomes 0.0016 0.0052 0.0055 0.0014 0.0018 0.0037 0.0026 0.0013 0.0057 0.0053 0.0015 0.0053 0.0019 0.0005 0.0014 0.0002 0.0021 0.0037
Punctate Nuclear 0.0023 0.0081 0.0041 0.0013 0.0047 0.0053 0.0044 0.0013 0.0019 0.0022 0.0012 0.0017 0.0011 0.0014 0.0015 0.0007 0.0011 0.0008
Vacuole 0.0864 0.0555 0.0621 0.0991 0.0684 0.1044 0.0618 0.0805 0.0987 0.0711 0.1332 0.1394 0.0573 0.1135 0.0677 0.0787 0.1478 0.0943
Vacuole Periphery 0.0063 0.005 0.0067 0.0184 0.0051 0.0083 0.0034 0.0039 0.0068 0.0061 0.0106 0.0075 0.0048 0.0052 0.0063 0.0087 0.007 0.0066

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.8131 15.1831 15.1515 20.3892 15.9463 11.1216 16.1456 19.4721 17.904 16.4093
Translational Efficiency 0.3104 0.4468 0.5786 0.4328 0.2962 0.5246 0.3765 0.324 0.4149 0.3139

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
141 1384 1742 1256 1940 1332 944 1436 2081 2716 2686 2692

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 913.39 780.29 809.31 902.94 725.38 769.59 836.42 909.02 738.12 775.04 818.84 906.18
Standard Deviation 157.55 109.04 104.09 123.87 78.06 99.20 102.94 122.69 97.96 104.47 104.49 123.28
Intensity Change Log 2 — -0.227221 -0.174539 -0.016601 — 0.085353 0.205491 0.325575 — -0.080457 0.006114 0.144938

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006277 0.011317 0.028895 0.220607 0.011419 0.010547 0.146823 0.144943 0.011071 0.010939 0.070341 0.180245
Bud Neck 0.009021 0.035038 0.017258 0.032839 0.072900 0.078841 0.027317 0.033689 0.068572 0.056520 0.020793 0.033292
Bud Site 0.072202 0.022024 0.022632 0.109774 0.017713 0.028054 0.065079 0.049072 0.021405 0.024981 0.037550 0.077393
Cell Periphery 0.001444 0.001539 0.007096 0.041267 0.001542 0.003816 0.022217 0.025416 0.001536 0.002656 0.012411 0.032811
Cytoplasm 0.215246 0.287076 0.354854 0.206306 0.246555 0.238672 0.242253 0.259161 0.244433 0.263337 0.315280 0.234500
Cytoplasmic Foci 0.097503 0.230346 0.106214 0.027245 0.126749 0.134578 0.065740 0.064839 0.124767 0.183379 0.091989 0.047299
Eisosomes 0.000995 0.003937 0.011778 0.095297 0.008017 0.008300 0.059704 0.080871 0.007542 0.006077 0.028621 0.087602
Endoplasmic Reticulum 0.006163 0.007947 0.036916 0.042450 0.009838 0.015172 0.070691 0.029862 0.009589 0.011490 0.048786 0.035735
Endosome 0.038471 0.064623 0.006517 0.004157 0.081194 0.100177 0.011029 0.012598 0.078299 0.082060 0.008103 0.008659
Golgi 0.024734 0.015884 0.006301 0.005378 0.021797 0.028082 0.017329 0.015700 0.021996 0.021866 0.010177 0.010884
Lipid Particles 0.006549 0.028180 0.047981 0.027750 0.041322 0.046405 0.069609 0.050002 0.038966 0.037118 0.055582 0.039620
Mitochondria 0.003842 0.003229 0.000759 0.001964 0.011019 0.016100 0.001725 0.004073 0.010533 0.009541 0.001099 0.003089
Mitotic Spindle 0.009700 0.001051 0.000718 0.001439 0.000570 0.001585 0.001113 0.001896 0.001189 0.001313 0.000857 0.001683
None 0.000192 0.000439 0.001327 0.000652 0.001445 0.000442 0.000583 0.001418 0.001360 0.000440 0.001065 0.001061
Nuclear Periphery 0.000469 0.000157 0.001353 0.000138 0.000193 0.000187 0.000404 0.000221 0.000212 0.000172 0.001019 0.000182
Nuclear Periphery Foci 0.002529 0.001114 0.007493 0.002767 0.000253 0.000463 0.005900 0.003533 0.000407 0.000795 0.006933 0.003176
Nucleolus 0.000473 0.000521 0.000342 0.000082 0.000677 0.000613 0.000198 0.000191 0.000663 0.000566 0.000292 0.000140
Nucleus 0.046258 0.030660 0.247866 0.053242 0.026569 0.019950 0.074733 0.056483 0.027903 0.025407 0.187018 0.054971
Peroxisomes 0.003682 0.022426 0.019537 0.012498 0.026189 0.026435 0.032431 0.028673 0.024664 0.024392 0.024069 0.021126
Vacuole 0.442900 0.230305 0.074024 0.113331 0.290234 0.236028 0.084274 0.136089 0.300578 0.233112 0.077627 0.125471
Vacuole Periphery 0.011351 0.002187 0.000138 0.000817 0.003805 0.005554 0.000849 0.001272 0.004317 0.003838 0.000388 0.001059

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.81 -6.06 -26.62 -27.63 -24.49 0.55 -18.15 -22.71 -22.80 0.26 0.07 -17.63 -35.12 -35.38 -19.26
Bud Neck -9.53 -3.61 -8.95 1.09 -6.51 -1.41 14.28 12.95 12.28 -2.50 3.81 17.76 13.11 10.27 -7.30
Bud Site 2.88 2.80 -2.81 -15.80 -15.08 -5.04 -8.99 -10.16 -5.99 2.65 -2.31 -6.30 -17.10 -16.20 -10.49
Cell Periphery -0.74 -9.45 -17.57 -17.55 -14.70 -4.23 -13.10 -15.30 -13.05 -1.35 -3.83 -16.00 -23.12 -21.85 -13.44
Cytoplasm -3.24 -6.12 0.49 9.54 17.70 1.11 0.68 -1.73 -2.57 -2.04 -3.05 -10.45 1.55 4.81 12.52
Cytoplasmic Foci -12.56 -1.71 6.00 37.53 21.35 -1.49 13.18 14.20 14.17 0.27 -12.83 7.86 21.30 35.59 14.96
Eisosomes -5.58 -10.56 -20.68 -19.93 -17.93 -0.30 -13.85 -19.49 -19.41 -4.10 1.88 -12.64 -26.91 -27.89 -18.10
Endoplasmic Reticulum -1.43 -13.53 -12.12 -12.13 -1.39 -3.57 -13.74 -10.03 -6.56 8.70 -1.82 -18.42 -14.71 -13.65 5.31
Endosome -4.62 4.13 4.51 22.26 3.73 -4.31 25.78 25.77 23.23 -0.97 -1.24 28.93 28.47 31.32 -0.54
Golgi 0.51 1.73 1.84 8.57 1.17 -3.37 3.11 3.94 6.39 0.85 -0.09 9.51 8.24 9.34 -0.72
Lipid Particles -12.80 -16.73 -9.73 0.48 6.90 -1.88 -6.49 -2.58 -0.81 4.25 0.85 -6.24 0.04 -0.80 6.25
Mitochondria -0.02 0.88 0.47 2.45 -2.68 -3.30 13.52 6.63 7.33 -2.39 1.01 15.31 9.56 7.41 -3.60
Mitotic Spindle 1.88 1.89 1.79 -0.49 -0.55 -1.18 -0.96 -1.72 -0.33 -0.87 -0.31 -0.00 -0.86 -0.60 -1.07
None -2.78 -5.88 -2.72 -1.23 2.74 1.61 1.42 -0.08 -1.86 -1.66 1.63 0.53 0.40 -2.13 -0.15
Nuclear Periphery 5.70 -5.80 5.87 0.51 9.37 0.11 -3.60 -0.78 -2.03 4.11 0.95 -8.50 0.48 -1.61 9.76
Nuclear Periphery Foci 4.23 -7.78 -1.03 -8.41 7.76 -3.13 -6.99 -9.26 -8.61 2.80 -5.75 -13.66 -13.30 -11.27 7.40
Nucleolus 0.15 0.91 2.15 11.99 8.92 0.51 6.92 7.57 3.79 0.05 1.48 7.82 11.27 7.31 4.04
Nucleus 2.86 -21.73 -0.41 -7.96 31.84 3.79 -12.71 -11.65 -13.97 4.23 1.86 -37.52 -12.88 -15.31 29.48
Peroxisomes -11.72 -10.82 -6.86 6.31 4.78 -0.15 -2.18 -0.78 -0.62 1.40 0.14 0.72 2.52 2.77 2.06
Vacuole 8.45 14.02 12.18 12.60 -7.74 5.93 25.43 17.81 10.18 -7.37 9.15 33.24 23.27 15.90 -11.47
Vacuole Periphery 1.45 1.87 1.73 2.85 -1.34 -4.31 6.37 6.75 8.57 -0.73 0.82 9.60 6.84 8.10 -1.95
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
ORF
Human Ortholog
Description Putative protein of unknown function; may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
Localization
Cell Percentages vacuole (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YHL008C

YHL008C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhl008c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available