ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 25 | 17 | 5 | 2 | 1 | 2 | 2 | 2 | 1 | 0 | 1 | 164 | 175 | 150 | 2 | 3 | 5 | 22 | 56 | 94 | 47 | 64 | 39 |
Bud | 4 | 14 | 8 | 6 | 2 | 5 | 9 | 11 | 11 | 5 | 5 | 8 | 23 | 23 | 9 | 1 | 2 | 0 | 3 | 2 | 1 | 0 | 6 | 10 |
Bud Neck | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 24 | 22 | 30 | 2 | 0 | 0 | 6 | 23 | 15 | 7 | 13 | 12 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 7 | 6 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 51 | 51 | 44 | 1 | 1 | 2 | 0 | 5 | 6 | 3 | 4 | 4 |
Cytoplasm | 233 | 344 | 312 | 272 | 302 | 253 | 338 | 382 | 268 | 405 | 267 | 379 | 1039 | 1039 | 1060 | 387 | 294 | 149 | 12 | 14 | 8 | 12 | 15 | 12 |
Endoplasmic Reticulum | 19 | 86 | 19 | 12 | 3 | 2 | 2 | 1 | 2 | 1 | 6 | 1 | 59 | 65 | 53 | 27 | 20 | 8 | 31 | 70 | 38 | 40 | 69 | 77 |
Endosome | 16 | 9 | 17 | 1 | 5 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 7 | 8 | 0 | 5 | 15 | 10 | 29 | 32 | 38 | 38 | 52 | 68 |
Golgi | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 7 | 13 | 11 | 7 | 12 | 33 |
Mitochondria | 6 | 4 | 7 | 11 | 5 | 47 | 57 | 43 | 52 | 69 | 42 | 84 | 1 | 1 | 0 | 24 | 15 | 3 | 19 | 35 | 51 | 72 | 73 | 57 |
Nucleus | 2 | 0 | 0 | 1 | 1 | 3 | 5 | 11 | 11 | 9 | 6 | 14 | 2 | 2 | 5 | 7 | 8 | 9 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 3 | 5 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 3 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 3 | 1 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 2 | 0 | 4 | 1 | 1 | 1 | 9 | 11 | 3 | 3 |
SpindlePole | 3 | 1 | 7 | 2 | 3 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 47 | 56 | 71 | 2 | 2 | 2 | 2 | 0 | 1 | 2 | 2 | 7 |
Vac/Vac Membrane | 79 | 23 | 25 | 20 | 53 | 42 | 81 | 87 | 81 | 116 | 38 | 87 | 8 | 9 | 4 | 67 | 100 | 50 | 298 | 305 | 207 | 229 | 425 | 439 |
Unique Cell Count | 339 | 477 | 386 | 308 | 350 | 306 | 412 | 480 | 342 | 502 | 315 | 477 | 1306 | 1317 | 1309 | 463 | 404 | 205 | 441 | 564 | 494 | 479 | 758 | 786 |
Labelled Cell Count | 374 | 515 | 423 | 333 | 384 | 362 | 497 | 542 | 428 | 609 | 365 | 578 | 1435 | 1456 | 1428 | 525 | 467 | 240 | 441 | 564 | 494 | 479 | 758 | 786 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 5.8 | 4.7 | 4.5 | 4.8 | 3.9 | 3.9 | 4.4 | 3.9 | 3.9 | 3.9 | 3.8 | 6.4 | 6.4 | 6.5 | 5.1 | 6.0 | 6.3 | 6.3 | 6.2 | 6.7 |
Std Deviation (1e-4) | 0.6 | 0.7 | 0.9 | 1.2 | 1.3 | 1.3 | 1.1 | 1.2 | 1.1 | 1.1 | 1.0 | 0.9 | 1.0 | 1.0 | 1.1 | 1.1 | 1.2 | 1.3 | 1.1 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.0 | -0.27 | -0.28 | -0.12 | -0.27 | -0.3 | -0.29 | -0.34 | 0.44 | 0.44 | 0.46 | 0.1 | 0.34 | 0.41 | 0.41 | 0.39 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 | 4.9 | 4.1 | -3.9 | -3.3 | -1.2 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | -0.4 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.1 | 2.6 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 2.2 | 1.8 | 0 | 0 | 0 |
Cytoplasm | 2.7 | 2.0 | 0.6 | 0.4 | -0.5 | -0.8 | -0.1 | 1.4 | -0.5 | -0.5 | -0.8 | 0.1 | 1.0 | -2.7 | -2.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.3 | 0 | -0.7 | 0.6 | 0 | -0.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.6 | -5.5 | 0 | -3.0 | -0.5 | 0.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 1.6 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 2.2 | 3.0 | 0 | 0 | 0 |
Vacuole | 0 | 3.8 | 3.2 | 5.5 | 5.1 | 6.6 | 6.7 | 2.6 | 5.1 | -7.3 | -7.2 | -8.2 | 3.7 | 7.0 | 6.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.303 | 1.8794 | 1.8491 | 1.7673 | 1.6807 | 1.6233 | 3.3222 | 4.1897 | 3.7128 | 3.4109 | 3.3602 | 4.0818 | 4.6025 | 4.793 | 5.072 | 6.0699 | 4.736 | 4.7153 |
Actin | 0.0126 | 0.0128 | 0.0068 | 0.0076 | 0.0025 | 0.0095 | 0.0141 | 0.0005 | 0.0112 | 0.0066 | 0.0027 | 0.0011 | 0.0043 | 0.0014 | 0.0106 | 0.003 | 0.0002 | 0.0023 |
Bud | 0.0021 | 0.0051 | 0.0099 | 0.0033 | 0.0245 | 0.0013 | 0.0011 | 0.002 | 0.002 | 0.002 | 0.002 | 0.0007 | 0.0026 | 0.0038 | 0.0025 | 0.0049 | 0.0007 | 0.0007 |
Bud Neck | 0.0036 | 0.0037 | 0.0045 | 0.0219 | 0.0024 | 0.0145 | 0.0025 | 0.0019 | 0.0046 | 0.0025 | 0.0086 | 0.0068 | 0.0014 | 0.002 | 0.003 | 0.0079 | 0.0029 | 0.0035 |
Bud Periphery | 0.0032 | 0.0049 | 0.0085 | 0.0146 | 0.0175 | 0.0042 | 0.0027 | 0.0028 | 0.0033 | 0.0066 | 0.0037 | 0.0024 | 0.0126 | 0.0093 | 0.0102 | 0.0292 | 0.0013 | 0.0037 |
Bud Site | 0.0218 | 0.0392 | 0.0197 | 0.0015 | 0.0065 | 0.0018 | 0.0175 | 0.0255 | 0.0129 | 0.0023 | 0.0077 | 0.0012 | 0.0043 | 0.012 | 0.0067 | 0.0021 | 0.0018 | 0.0008 |
Cell Periphery | 0.0052 | 0.004 | 0.003 | 0.0023 | 0.0045 | 0.0024 | 0.0089 | 0.0034 | 0.0031 | 0.003 | 0.0031 | 0.0024 | 0.0079 | 0.0055 | 0.0068 | 0.0108 | 0.0015 | 0.0035 |
Cytoplasm | 0.2655 | 0.2891 | 0.2783 | 0.2779 | 0.3248 | 0.3104 | 0.2711 | 0.3126 | 0.2517 | 0.2902 | 0.3336 | 0.2989 | 0.2409 | 0.331 | 0.2637 | 0.2257 | 0.2624 | 0.3499 |
Cytoplasmic Foci | 0.0622 | 0.0853 | 0.0798 | 0.0749 | 0.1011 | 0.0976 | 0.0968 | 0.0585 | 0.0892 | 0.0998 | 0.0813 | 0.0908 | 0.0694 | 0.0351 | 0.0525 | 0.0227 | 0.048 | 0.0571 |
Eisosomes | 0.0014 | 0.0012 | 0.0005 | 0.0001 | 0.0003 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0218 | 0.0175 | 0.0303 | 0.0161 | 0.0206 | 0.023 | 0.0241 | 0.0299 | 0.0251 | 0.0173 | 0.0139 | 0.0324 | 0.2365 | 0.1556 | 0.2228 | 0.3276 | 0.2035 | 0.1861 |
Endosome | 0.1521 | 0.1811 | 0.2015 | 0.3446 | 0.1659 | 0.1726 | 0.1392 | 0.1765 | 0.195 | 0.2007 | 0.2368 | 0.1825 | 0.1651 | 0.0997 | 0.1364 | 0.1436 | 0.2104 | 0.1289 |
Golgi | 0.0073 | 0.0202 | 0.0142 | 0.0281 | 0.0108 | 0.0108 | 0.0243 | 0.0094 | 0.0185 | 0.0241 | 0.0153 | 0.0073 | 0.0141 | 0.0067 | 0.011 | 0.0066 | 0.0045 | 0.006 |
Lipid Particles | 0.021 | 0.0142 | 0.0139 | 0.0095 | 0.0124 | 0.0188 | 0.0301 | 0.0203 | 0.0151 | 0.0192 | 0.0086 | 0.024 | 0.0303 | 0.0119 | 0.0197 | 0.0099 | 0.0052 | 0.0094 |
Mitochondria | 0.0202 | 0.0075 | 0.016 | 0.0066 | 0.0105 | 0.0101 | 0.0189 | 0.0051 | 0.0099 | 0.008 | 0.0152 | 0.0035 | 0.0084 | 0.003 | 0.0041 | 0.0031 | 0.0032 | 0.0038 |
None | 0.2916 | 0.2242 | 0.2217 | 0.0622 | 0.201 | 0.1881 | 0.2684 | 0.254 | 0.2337 | 0.2217 | 0.1147 | 0.1836 | 0.1277 | 0.1858 | 0.16 | 0.1051 | 0.0855 | 0.1295 |
Nuclear Periphery | 0.0047 | 0.0046 | 0.0051 | 0.0053 | 0.0037 | 0.0055 | 0.0029 | 0.0034 | 0.0052 | 0.0064 | 0.0024 | 0.0033 | 0.0062 | 0.0076 | 0.0083 | 0.0064 | 0.0044 | 0.0059 |
Nucleolus | 0.0015 | 0.0033 | 0.0025 | 0.0006 | 0.0043 | 0.0016 | 0.0014 | 0.0017 | 0.0016 | 0.0012 | 0.0008 | 0.001 | 0.0006 | 0.0014 | 0.0009 | 0.0005 | 0.0007 | 0.0005 |
Nucleus | 0.0057 | 0.0085 | 0.0054 | 0.0027 | 0.0067 | 0.006 | 0.0032 | 0.0054 | 0.0044 | 0.0032 | 0.0028 | 0.004 | 0.0021 | 0.0073 | 0.0036 | 0.0026 | 0.0056 | 0.003 |
Peroxisomes | 0.0016 | 0.0052 | 0.0055 | 0.0014 | 0.0018 | 0.0037 | 0.0026 | 0.0013 | 0.0057 | 0.0053 | 0.0015 | 0.0053 | 0.0019 | 0.0005 | 0.0014 | 0.0002 | 0.0021 | 0.0037 |
Punctate Nuclear | 0.0023 | 0.0081 | 0.0041 | 0.0013 | 0.0047 | 0.0053 | 0.0044 | 0.0013 | 0.0019 | 0.0022 | 0.0012 | 0.0017 | 0.0011 | 0.0014 | 0.0015 | 0.0007 | 0.0011 | 0.0008 |
Vacuole | 0.0864 | 0.0555 | 0.0621 | 0.0991 | 0.0684 | 0.1044 | 0.0618 | 0.0805 | 0.0987 | 0.0711 | 0.1332 | 0.1394 | 0.0573 | 0.1135 | 0.0677 | 0.0787 | 0.1478 | 0.0943 |
Vacuole Periphery | 0.0063 | 0.005 | 0.0067 | 0.0184 | 0.0051 | 0.0083 | 0.0034 | 0.0039 | 0.0068 | 0.0061 | 0.0106 | 0.0075 | 0.0048 | 0.0052 | 0.0063 | 0.0087 | 0.007 | 0.0066 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.8131 | 15.1831 | 15.1515 | 20.3892 | 15.9463 | 11.1216 | 16.1456 | 19.4721 | 17.904 | 16.4093 |
Translational Efficiency | 0.3104 | 0.4468 | 0.5786 | 0.4328 | 0.2962 | 0.5246 | 0.3765 | 0.324 | 0.4149 | 0.3139 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
141 | 1384 | 1742 | 1256 | 1940 | 1332 | 944 | 1436 | 2081 | 2716 | 2686 | 2692 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 913.39 | 780.29 | 809.31 | 902.94 | 725.38 | 769.59 | 836.42 | 909.02 | 738.12 | 775.04 | 818.84 | 906.18 |
Standard Deviation | 157.55 | 109.04 | 104.09 | 123.87 | 78.06 | 99.20 | 102.94 | 122.69 | 97.96 | 104.47 | 104.49 | 123.28 |
Intensity Change Log 2 | — | -0.227221 | -0.174539 | -0.016601 | — | 0.085353 | 0.205491 | 0.325575 | — | -0.080457 | 0.006114 | 0.144938 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.006277 | 0.011317 | 0.028895 | 0.220607* | 0.011419 | 0.010547 | 0.146823* | 0.144943* | 0.011071 | 0.010939 | 0.070341 | 0.180245* |
Bud Neck | 0.009021 | 0.035038 | 0.017258 | 0.032839 | 0.072900 | 0.078841 | 0.027317 | 0.033689 | 0.068572 | 0.056520 | 0.020793 | 0.033292 |
Bud Site | 0.072202 | 0.022024 | 0.022632 | 0.109774* | 0.017713 | 0.028054 | 0.065079 | 0.049072 | 0.021405 | 0.024981 | 0.037550 | 0.077393 |
Cell Periphery | 0.001444 | 0.001539 | 0.007096 | 0.041267 | 0.001542 | 0.003816 | 0.022217 | 0.025416 | 0.001536 | 0.002656 | 0.012411 | 0.032811 |
Cytoplasm | 0.215246* | 0.287076* | 0.354854* | 0.206306* | 0.246555* | 0.238672* | 0.242253* | 0.259161* | 0.244433* | 0.263337* | 0.315280* | 0.234500* |
Cytoplasmic Foci | 0.097503 | 0.230346* | 0.106214 | 0.027245 | 0.126749 | 0.134578 | 0.065740 | 0.064839 | 0.124767 | 0.183379* | 0.091989 | 0.047299 |
Eisosomes | 0.000995 | 0.003937 | 0.011778 | 0.095297 | 0.008017 | 0.008300 | 0.059704 | 0.080871 | 0.007542 | 0.006077 | 0.028621 | 0.087602 |
Endoplasmic Reticulum | 0.006163 | 0.007947 | 0.036916 | 0.042450 | 0.009838 | 0.015172 | 0.070691 | 0.029862 | 0.009589 | 0.011490 | 0.048786 | 0.035735 |
Endosome | 0.038471 | 0.064623 | 0.006517 | 0.004157 | 0.081194 | 0.100177 | 0.011029 | 0.012598 | 0.078299 | 0.082060 | 0.008103 | 0.008659 |
Golgi | 0.024734 | 0.015884 | 0.006301 | 0.005378 | 0.021797 | 0.028082 | 0.017329 | 0.015700 | 0.021996 | 0.021866 | 0.010177 | 0.010884 |
Lipid Particles | 0.006549 | 0.028180 | 0.047981 | 0.027750 | 0.041322 | 0.046405 | 0.069609 | 0.050002 | 0.038966 | 0.037118 | 0.055582 | 0.039620 |
Mitochondria | 0.003842 | 0.003229 | 0.000759 | 0.001964 | 0.011019 | 0.016100 | 0.001725 | 0.004073 | 0.010533 | 0.009541 | 0.001099 | 0.003089 |
Mitotic Spindle | 0.009700 | 0.001051 | 0.000718 | 0.001439 | 0.000570 | 0.001585 | 0.001113 | 0.001896 | 0.001189 | 0.001313 | 0.000857 | 0.001683 |
None | 0.000192 | 0.000439 | 0.001327 | 0.000652 | 0.001445 | 0.000442 | 0.000583 | 0.001418 | 0.001360 | 0.000440 | 0.001065 | 0.001061 |
Nuclear Periphery | 0.000469 | 0.000157 | 0.001353 | 0.000138 | 0.000193 | 0.000187 | 0.000404 | 0.000221 | 0.000212 | 0.000172 | 0.001019 | 0.000182 |
Nuclear Periphery Foci | 0.002529 | 0.001114 | 0.007493 | 0.002767 | 0.000253 | 0.000463 | 0.005900 | 0.003533 | 0.000407 | 0.000795 | 0.006933 | 0.003176 |
Nucleolus | 0.000473 | 0.000521 | 0.000342 | 0.000082 | 0.000677 | 0.000613 | 0.000198 | 0.000191 | 0.000663 | 0.000566 | 0.000292 | 0.000140 |
Nucleus | 0.046258 | 0.030660 | 0.247866* | 0.053242 | 0.026569 | 0.019950 | 0.074733 | 0.056483 | 0.027903 | 0.025407 | 0.187018* | 0.054971 |
Peroxisomes | 0.003682 | 0.022426 | 0.019537 | 0.012498 | 0.026189 | 0.026435 | 0.032431 | 0.028673 | 0.024664 | 0.024392 | 0.024069 | 0.021126 |
Vacuole | 0.442900* | 0.230305* | 0.074024 | 0.113331 | 0.290234* | 0.236028* | 0.084274 | 0.136089 | 0.300578* | 0.233112* | 0.077627 | 0.125471 |
Vacuole Periphery | 0.011351 | 0.002187 | 0.000138 | 0.000817 | 0.003805 | 0.005554 | 0.000849 | 0.001272 | 0.004317 | 0.003838 | 0.000388 | 0.001059 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.81 | -6.06 | -26.62* | -27.63* | -24.49* | 0.55 | -18.15 | -22.71 | -22.80* | 0.26 | 0.07 | -17.63 | -35.12* | -35.38* | -19.26 |
Bud Neck | -9.53 | -3.61 | -8.95 | 1.09 | -6.51 | -1.41 | 14.28 | 12.95 | 12.28 | -2.50 | 3.81 | 17.76 | 13.11 | 10.27 | -7.30 |
Bud Site | 2.88 | 2.80 | -2.81 | -15.80 | -15.08 | -5.04 | -8.99 | -10.16 | -5.99 | 2.65 | -2.31 | -6.30 | -17.10 | -16.20 | -10.49 |
Cell Periphery | -0.74 | -9.45 | -17.57 | -17.55 | -14.70 | -4.23 | -13.10 | -15.30 | -13.05 | -1.35 | -3.83 | -16.00 | -23.12 | -21.85 | -13.44 |
Cytoplasm | -3.24 | -6.12 | 0.49 | 9.54 | 17.70 | 1.11 | 0.68 | -1.73 | -2.57 | -2.04 | -3.05 | -10.45 | 1.55 | 4.81 | 12.52 |
Cytoplasmic Foci | -12.56 | -1.71 | 6.00 | 37.53 | 21.35 | -1.49 | 13.18 | 14.20 | 14.17 | 0.27 | -12.83 | 7.86 | 21.30 | 35.59 | 14.96 |
Eisosomes | -5.58 | -10.56 | -20.68 | -19.93 | -17.93 | -0.30 | -13.85 | -19.49 | -19.41 | -4.10 | 1.88 | -12.64 | -26.91 | -27.89 | -18.10 |
Endoplasmic Reticulum | -1.43 | -13.53 | -12.12 | -12.13 | -1.39 | -3.57 | -13.74 | -10.03 | -6.56 | 8.70 | -1.82 | -18.42 | -14.71 | -13.65 | 5.31 |
Endosome | -4.62 | 4.13 | 4.51 | 22.26 | 3.73 | -4.31 | 25.78 | 25.77 | 23.23 | -0.97 | -1.24 | 28.93 | 28.47 | 31.32 | -0.54 |
Golgi | 0.51 | 1.73 | 1.84 | 8.57 | 1.17 | -3.37 | 3.11 | 3.94 | 6.39 | 0.85 | -0.09 | 9.51 | 8.24 | 9.34 | -0.72 |
Lipid Particles | -12.80 | -16.73 | -9.73 | 0.48 | 6.90 | -1.88 | -6.49 | -2.58 | -0.81 | 4.25 | 0.85 | -6.24 | 0.04 | -0.80 | 6.25 |
Mitochondria | -0.02 | 0.88 | 0.47 | 2.45 | -2.68 | -3.30 | 13.52 | 6.63 | 7.33 | -2.39 | 1.01 | 15.31 | 9.56 | 7.41 | -3.60 |
Mitotic Spindle | 1.88 | 1.89 | 1.79 | -0.49 | -0.55 | -1.18 | -0.96 | -1.72 | -0.33 | -0.87 | -0.31 | -0.00 | -0.86 | -0.60 | -1.07 |
None | -2.78 | -5.88 | -2.72 | -1.23 | 2.74 | 1.61 | 1.42 | -0.08 | -1.86 | -1.66 | 1.63 | 0.53 | 0.40 | -2.13 | -0.15 |
Nuclear Periphery | 5.70 | -5.80 | 5.87 | 0.51 | 9.37 | 0.11 | -3.60 | -0.78 | -2.03 | 4.11 | 0.95 | -8.50 | 0.48 | -1.61 | 9.76 |
Nuclear Periphery Foci | 4.23 | -7.78 | -1.03 | -8.41 | 7.76 | -3.13 | -6.99 | -9.26 | -8.61 | 2.80 | -5.75 | -13.66 | -13.30 | -11.27 | 7.40 |
Nucleolus | 0.15 | 0.91 | 2.15 | 11.99 | 8.92 | 0.51 | 6.92 | 7.57 | 3.79 | 0.05 | 1.48 | 7.82 | 11.27 | 7.31 | 4.04 |
Nucleus | 2.86 | -21.73 | -0.41 | -7.96 | 31.84 | 3.79 | -12.71 | -11.65 | -13.97 | 4.23 | 1.86 | -37.52 | -12.88 | -15.31 | 29.48 |
Peroxisomes | -11.72 | -10.82 | -6.86 | 6.31 | 4.78 | -0.15 | -2.18 | -0.78 | -0.62 | 1.40 | 0.14 | 0.72 | 2.52 | 2.77 | 2.06 |
Vacuole | 8.45 | 14.02 | 12.18 | 12.60 | -7.74 | 5.93 | 25.43 | 17.81 | 10.18 | -7.37 | 9.15 | 33.24 | 23.27 | 15.90 | -11.47 |
Vacuole Periphery | 1.45 | 1.87 | 1.73 | 2.85 | -1.34 | -4.31 | 6.37 | 6.75 | 8.57 | -0.73 | 0.82 | 9.60 | 6.84 | 8.10 | -1.95 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|