Standard name
Human Ortholog
Description Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; null exhibits disrupted mitochondrial metabolism and low cardiolipin content, strongly correlated with overproduction of inositol; binds to phosphatidic acid
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 – – – 0.06 0.15 0.07 0 0 – 0 0.08 0.16 0.17 0.08 0.14 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – – – 0.09 0.15 0.11 0.35 0.4 – 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.85 0.85 – – – 0.77 0.7 0.88 0.69 0.75 – 0.76 0.89 0.83 0.81 0.81 0.7 0.82 0.64 0.76 0.56 0.77 0.77 0.67
Nuclear Periphery 0.22 0.07 – – – 0.31 0.15 0.14 0.08 0.07 – 0.08 0.1 0 0 0 0 0 0.22 0.07 0.3 0.08 0 0
Nucleolus 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0.05 0 0.05 0 0 0.06 0 0 0
Peroxisomes 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.15 – – – 0.2 0.1 0.09 0.23 0.09 – 0 0 0 0 0.06 0.09 0.06 0.09 0.1 0.06 0.06 0.16 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – – 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Neck 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – – 0 0 0 0 1 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 0 – – – 2 3 4 0 1 – 1 15 37 46 6 12 5 1 0 0 3 2 4
Endoplasmic Reticulum 0 2 – – – 0 0 0 0 0 – 0 0 0 0 1 5 1 0 0 0 0 0 1
Endosome 0 0 – – – 0 0 0 0 0 – 0 0 0 0 1 2 2 0 0 0 0 1 3
Golgi 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 4 3 – – – 3 3 6 9 27 – 6 1 2 1 1 0 1 0 0 0 0 0 0
Nucleus 112 62 – – – 27 14 50 18 50 – 19 166 197 222 62 62 80 82 69 19 81 168 140
Nuclear Periphery 29 5 – – – 11 3 8 2 5 – 2 19 6 2 1 2 1 28 6 10 8 8 8
Nucleolus 0 1 – – – 0 0 0 0 1 – 0 0 2 1 4 3 5 2 2 1 0 1 4
Peroxisomes 0 0 – – – 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 – – – 0 0 0 0 0 – 0 1 2 0 1 1 0 0 0 0 1 0 2
Vac/Vac Membrane 14 11 – – – 7 2 5 6 6 – 1 4 6 8 5 8 6 12 9 1 6 34 38
Unique Cell Count 131 73 35 20 57 26 67 25 187 237 273 77 88 97 130 91 34 106 220 210
Labelled Cell Count 165 84 50 25 73 35 91 29 207 252 280 82 96 101 130 91 34 106 220 210


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 7.1 – – – 5.9 6.6 5.5 4.8 4.6 – 4.5 8.5 8.3 8.2 11.1 10.4 10.0 8.2 9.0 9.6
Std Deviation (1e-4) 0.9 1.4 – – – 1.8 2.5 1.9 0.9 0.8 – 0.7 2.1 2.3 2.3 2.5 2.8 2.7 1.6 1.4 1.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.547 5.8294 5.1098 4.8453 5.0457 5.3837 4.3358 4.4487 4.1589 4.0939 3.1358 3.6882 3.1742 3.297 2.9562 3.2125 2.634 2.8541
Actin 0.0005 0.0001 0.0001 0.0002 0.0002 0.0008 0.046 0.0001 0.0012 0.0063 0.0339 0.0122 0.0051 0.0002 0.0002 0.0002 0.0003 0.0003
Bud 0.0001 0 0 0 0 0 0.0011 0.0001 0 0.0008 0.0003 0.0002 0.0003 0 0 0 0 0
Bud Neck 0.0004 0.0001 0 0.0003 0.0003 0.0009 0.0028 0.0003 0.0001 0.0006 0.0004 0.002 0.0007 0.0001 0 0.0001 0.0002 0.0006
Bud Periphery 0.0001 0 0 0.0001 0 0.0001 0.0019 0.0001 0 0.0027 0.0007 0.0006 0.0012 0 0 0 0 0.0001
Bud Site 0.0026 0 0 0 0 0 0.0125 0.0022 0.0001 0.0039 0.0003 0.0003 0.0015 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.0006 0.0001 0 0.0004 0.0002 0.0001 0.0006 0 0 0 0 0
Cytoplasm 0.0028 0.0012 0.0015 0.0011 0.0175 0.0005 0.0047 0.001 0.0003 0.0006 0.0005 0.001 0.0007 0.0007 0.0008 0.001 0.0005 0.0006
Cytoplasmic Foci 0.0027 0.0001 0.0001 0.0001 0.0004 0.0006 0.0386 0.0003 0.0025 0.0002 0.0047 0.0021 0.0002 0.0001 0 0 0 0
Eisosomes 0 0 0 0 0 0 0.0006 0 0 0.0003 0.0005 0.0001 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0012 0.003 0.0007 0.008 0.012 0.0011 0.0126 0.0005 0.0002 0.0005 0.0027 0.0017 0.0023 0.0022 0.0073 0.0091 0.0276 0.0011
Endosome 0.0027 0.0011 0.0006 0.004 0.004 0.0006 0.049 0.0003 0.0008 0.0014 0.077 0.0131 0.0016 0.0014 0.0009 0.0008 0.0005 0.0003
Golgi 0.0004 0 0 0.0001 0 0.0003 0.0189 0.0001 0.0021 0.0009 0.0629 0.0066 0.0005 0.0002 0 0 0 0.0001
Lipid Particles 0.0006 0.0002 0.0001 0.0002 0.0001 0.0009 0.0286 0.0003 0.0015 0.0008 0.0079 0.0026 0.002 0.0025 0.0001 0 0 0.001
Mitochondria 0.0006 0.0004 0.0006 0.0006 0.0006 0.0009 0.0327 0.0007 0.0007 0.0241 0.0249 0.0148 0.0011 0.0007 0.0005 0.0005 0.0007 0.0007
None 0.0049 0.0001 0.0009 0.0001 0.0011 0.0001 0.0019 0 0 0.0002 0.0002 0.0004 0.0002 0 0.0001 0.0001 0.0001 0.0075
Nuclear Periphery 0.2459 0.326 0.276 0.2185 0.2888 0.2871 0.2509 0.2399 0.2488 0.1928 0.2735 0.3324 0.4568 0.4221 0.3768 0.3414 0.3605 0.3808
Nucleolus 0.0013 0.0017 0.0014 0.0005 0.0018 0.0035 0.0091 0.0076 0.0007 0.0016 0.0044 0.0023 0.0009 0.0006 0.0007 0.0004 0.0011 0.0014
Nucleus 0.7252 0.6613 0.712 0.7605 0.6677 0.6972 0.4408 0.7424 0.7347 0.7576 0.4953 0.5944 0.5179 0.564 0.6087 0.6429 0.6045 0.6005
Peroxisomes 0.0004 0 0 0 0 0.0015 0.0163 0.0001 0.0039 0.0004 0.0006 0.0007 0 0.0001 0 0 0 0
Punctate Nuclear 0.0057 0.0006 0.0031 0.0004 0.0015 0.0011 0.0135 0.0004 0.0001 0.0004 0.0005 0.0041 0.0006 0.0003 0.0005 0.0006 0.0003 0.0005
Vacuole 0.0004 0.0015 0.0003 0.0031 0.0016 0.0006 0.0073 0.001 0.0002 0.0014 0.0024 0.003 0.0014 0.0006 0.0004 0.0005 0.0004 0.0009
Vacuole Periphery 0.0017 0.0026 0.0024 0.0022 0.0023 0.0021 0.0096 0.0023 0.0021 0.0021 0.0062 0.0053 0.0041 0.0041 0.0031 0.0023 0.0033 0.0037

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.4128 30.8496 33.7352 33.574 38.2674 42.6595 44.9411 47.6452 41.2097 31.9143
Translational Efficiency 0.6639 0.886 0.7499 0.7541 0.5266 0.627 0.5136 0.6851 0.6956 0.5933

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
216 215 1974 1565 2640 742 250 786 2856 957 2224 2351

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 894.95 844.94 1139.91 1127.65 836.28 914.35 1179.35 1154.70 840.72 898.76 1144.34 1136.69
Standard Deviation 115.58 148.39 170.68 172.95 118.59 113.71 142.69 185.40 119.38 125.75 168.23 177.67
Intensity Change Log 2 — -0.082958 0.349041 0.333440 — 0.128760 0.495934 0.465460 — 0.023196 0.421867 0.398723

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000512 0.000861 0.000323 0.000907 0.000112 0.000336 0.000773 0.000995 0.000142 0.000454 0.000373 0.000937
Bud Neck 0.001031 0.003065 0.002129 0.023555 0.000902 0.000842 0.001621 0.021932 0.000912 0.001341 0.002072 0.023013
Bud Site 0.000353 0.001685 0.000191 0.006972 0.000131 0.000210 0.000324 0.005141 0.000148 0.000541 0.000206 0.006360
Cell Periphery 0.000131 0.000159 0.000050 0.000174 0.000162 0.000055 0.000085 0.000195 0.000159 0.000078 0.000054 0.000181
Cytoplasm 0.006397 0.026802 0.005041 0.017288 0.004875 0.003560 0.009502 0.013853 0.004990 0.008782 0.005543 0.016140
Cytoplasmic Foci 0.000982 0.004824 0.000088 0.007883 0.000247 0.001248 0.001023 0.010876 0.000302 0.002052 0.000193 0.008883
Eisosomes 0.000019 0.000019 0.000003 0.000024 0.000006 0.000013 0.000028 0.000024 0.000007 0.000015 0.000006 0.000024
Endoplasmic Reticulum 0.038507 0.043533 0.032236 0.035953 0.038699 0.039728 0.058554 0.037560 0.038684 0.040583 0.035195 0.036490
Endosome 0.001494 0.001818 0.001738 0.009215 0.000879 0.001566 0.001695 0.010475 0.000926 0.001623 0.001733 0.009636
Golgi 0.000265 0.000151 0.000035 0.003898 0.000012 0.001053 0.000063 0.001158 0.000031 0.000850 0.000038 0.002982
Lipid Particles 0.001089 0.000828 0.000128 0.001020 0.000164 0.000848 0.001665 0.003388 0.000234 0.000843 0.000301 0.001812
Mitochondria 0.004335 0.003551 0.002282 0.009350 0.000690 0.005326 0.002604 0.006533 0.000966 0.004927 0.002318 0.008408
Mitotic Spindle 0.004926 0.000806 0.000669 0.075982 0.000027 0.001627 0.000218 0.063208 0.000397 0.001443 0.000619 0.071712
None 0.003511 0.011800 0.000534 0.003669 0.002846 0.002460 0.001518 0.002471 0.002896 0.004558 0.000645 0.003268
Nuclear Periphery 0.197877* 0.147026* 0.791577* 0.386976* 0.134964* 0.242853* 0.496761* 0.373472* 0.139722* 0.221325* 0.758437* 0.382461*
Nuclear Periphery Foci 0.008265 0.021745 0.038327 0.094294 0.001386 0.009784 0.063245 0.181590 0.001906 0.012471 0.041128 0.123479
Nucleolus 0.023152 0.032800 0.010313 0.044479 0.009990 0.019523 0.020591 0.052906 0.010985 0.022506 0.011468 0.047296
Nucleus 0.703438* 0.693327* 0.107123 0.240001* 0.800284* 0.661072* 0.327190* 0.183893* 0.792959* 0.668318* 0.131860* 0.221243*
Peroxisomes 0.001021 0.001210 0.000139 0.002094 0.000109 0.000968 0.000989 0.002619 0.000178 0.001022 0.000234 0.002270
Vacuole 0.001867 0.002754 0.005232 0.029786 0.002681 0.004578 0.010525 0.021314 0.002620 0.004168 0.005827 0.026954
Vacuole Periphery 0.000827 0.001235 0.001842 0.006479 0.000837 0.002349 0.001026 0.006395 0.000836 0.002099 0.001750 0.006451

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.49 1.86 -6.30 -2.70 -12.47 -6.54 -9.32 -10.12 -7.74 -3.14 -8.27 -11.64 -18.38 -11.10 -13.48
Bud Neck -2.80 -3.42 -19.18 -16.83 -18.55 0.31 -3.05 -12.97 -12.99 -12.40 -2.38 -6.08 -23.36 -22.85 -22.15
Bud Site -1.98 0.53 -8.66 -5.90 -8.85 -1.41 -2.84 -8.94 -8.81 -8.46 -2.46 -1.93 -11.32 -10.43 -11.13
Cell Periphery -0.44 1.32 -0.95 -0.81 -9.27 4.92 2.49 -1.72 -8.07 -4.75 3.74 4.89 -1.59 -8.52 -11.37
Cytoplasm -2.94 0.44 -4.33 1.05 -9.52 1.53 -1.41 -6.17 -6.88 -1.09 -2.33 -0.96 -10.78 -4.95 -9.85
Cytoplasmic Foci -1.16 1.90 -5.17 -0.74 -6.20 -1.90 -1.82 -4.97 -4.35 -4.39 -2.03 0.44 -7.78 -4.75 -7.86
Eisosomes -0.13 5.72 -4.38 -3.39 -16.78 -4.32 -6.25 -8.83 -5.01 0.73 -4.95 0.58 -16.75 -8.00 -15.91
Endoplasmic Reticulum -0.79 1.26 1.48 2.47 0.48 -0.39 -4.29 1.11 1.26 4.55 -0.78 1.68 3.15 3.28 1.37
Endosome -0.42 -0.45 -6.52 -6.94 -7.90 -1.60 -4.33 -8.37 -7.26 -7.40 -1.97 -4.82 -12.23 -9.92 -10.64
Golgi 0.73 1.51 -2.99 -3.17 -3.31 -1.77 -7.03 -6.29 -0.18 -5.97 -1.79 -0.89 -3.98 -1.94 -3.96
Lipid Particles 0.67 2.86 -0.31 -1.59 -10.20 -5.39 -6.29 -3.20 -2.45 -1.48 -5.42 -1.92 -4.98 -2.96 -4.70
Mitochondria 0.42 1.12 -2.42 -5.48 -8.51 -3.82 -7.85 -4.66 -0.75 -3.13 -4.12 -7.29 -10.65 -2.85 -8.60
Mitotic Spindle 1.41 1.41 -13.72 -16.08 -16.08 -2.12 -2.82 -10.48 -10.16 -10.45 -1.71 -0.88 -19.24 -18.81 -19.08
None -2.15 1.97 -0.18 2.20 -4.52 0.24 1.62 0.37 -0.06 -1.37 -1.42 5.63 -0.83 0.98 -5.43
Nuclear Periphery 1.86 -25.77 -3.59 -7.14 46.08* -8.19 -14.05 -14.66 -4.75 5.87 -7.14 -72.60 -18.34 -6.02 46.51*
Nuclear Periphery Foci -2.30 -12.36 -16.44 -8.42 -9.40 -4.74 -8.93 -20.57 -19.06 -9.81 -5.65 -22.55 -27.61 -22.41 -15.87
Nucleolus -1.82 4.10 -7.35 -4.25 -13.82 -5.70 -3.81 -10.58 -8.30 -7.61 -6.94 -0.48 -17.22 -11.42 -16.53
Nucleus 0.34 24.55 15.51 16.25 -20.73 9.75 18.94 49.25* 26.23 4.62 9.94 83.89* 59.42* 29.30 -17.02
Peroxisomes -0.40 2.58 -1.99 -1.66 -4.42 -2.41 -7.24 -5.12 -2.76 -3.37 -2.92 -1.74 -6.25 -2.84 -6.08
Vacuole -2.02 -6.68 -15.54 -14.98 -13.83 -2.08 -4.98 -9.12 -7.87 -3.93 -2.16 -6.55 -17.55 -15.71 -15.18
Vacuole Periphery -1.19 -2.87 -6.26 -5.47 -4.46 -2.18 -0.99 -4.92 -2.86 -4.79 -2.30 -3.11 -7.96 -4.15 -5.84
Standard name
Human Ortholog
Description Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; null exhibits disrupted mitochondrial metabolism and low cardiolipin content, strongly correlated with overproduction of inositol; binds to phosphatidic acid
Localization
Cell Percentages nuclear periphery (38%), nucleus (28%), mixed (32%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Opi1

Opi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Opi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available