ORF
Human Ortholog
Description Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; SWAT-GFP fusion protein localizes to the cell periphery, while mCherry fusion localizes to both the cell periphery and vacuole; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.05 0.06 0.06
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0.05 0 0 0 0 – 0 0 0 0.06 0.06 0 0.25 0.3 0.34 0 0.08 0.13 0.22 0.16 0.15
Cytoplasm 1.0 0.97 0.96 0.95 0.97 0.92 0.91 0.84 – 0.84 0.62 0.81 1.0 0.99 1.0 0.77 0.78 0.79 0.56 0.65 0.51 0.46 0.52 0.54
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.12 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.05 0 0.16 0.2 0.23 – 0.22 0.5 0.37 0 0 0 0.08 0 0 0.17 0.05 0.12 0.14 0.11 0.1
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 0 0 0 0 – 1 2 0 0 1 0 5 6 3 1 13 14 14 20 21
Bud 0 0 2 1 2 5 9 8 – 15 15 20 0 1 0 5 3 3 0 1 0 1 7 5
Bud Neck 0 1 2 1 7 9 5 8 – 10 3 7 5 1 4 4 1 0 0 6 3 13 13 13
Bud Site 0 0 0 0 0 2 0 0 – 1 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 6 10 7 7 10 10 8 – 16 10 12 32 27 24 45 54 49 0 14 18 62 53 57
Cytoplasm 17 165 132 126 311 316 512 445 – 514 230 472 506 465 610 141 140 114 21 122 74 132 174 199
Endoplasmic Reticulum 0 2 0 0 0 0 0 1 – 2 5 1 2 4 2 10 21 11 0 3 6 2 5 6
Endosome 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 2 1 2 2 1 3 1 3 6
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 1 0 0 0 2 1 2
Mitochondria 1 3 3 7 13 54 112 122 – 135 185 217 0 1 0 14 7 3 6 9 16 38 38 37
Nucleus 0 0 1 1 0 0 3 0 – 1 0 2 2 0 0 0 0 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 4 7 5 – 15 2 12 0 0 0 11 3 0 0 0 0 0 2 1
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 1 0 0 0 1 0 1 0 0 7 0 8 8 14
Unique Cell Count 17 170 137 132 319 343 562 531 615 373 586 508 471 612 182 180 145 38 187 145 286 336 371
Labelled Cell Count 18 178 151 145 342 400 659 598 710 454 746 547 500 641 238 238 186 38 187 145 286 336 371


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.5 3.2 3.2 3.0 3.0 2.6 2.9 – 2.7 2.4 2.5 5.2 4.9 5.1 5.7 6.0 6.0 5.4 5.5 5.6
Std Deviation (1e-4) 1.8 1.2 0.6 1.4 0.8 1.2 0.8 1.0 – 0.5 0.3 0.6 0.8 0.7 0.8 0.9 1.5 1.2 1.1 1.0 1.1
Intensity Change (Log2) – – – 0.01 -0.09 -0.08 -0.3 -0.14 – -0.22 -0.37 -0.32 0.72 0.64 0.7 0.84 0.92 0.93 0.77 0.79 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5039 -0.158 -0.3924 -0.4134 -0.3731 -0.1584 1.472 1.5782 1.5787 1.3591 1.3165 1.5818 -1.4399 -0.7744 -0.9808 -0.6365 -0.9845 -1.0664
Actin 0.0082 0.0011 0.0034 0.0007 0.0151 0.0068 0.0508 0.0024 0.0159 0.0005 0.0224 0.0047 0.0139 0.0006 0.0019 0.0004 0.0072 0.0028
Bud 0.0002 0.0076 0.0027 0.0001 0.0002 0.0007 0.0026 0.0046 0.0013 0.0003 0.0076 0.0008 0.0002 0.0012 0.0013 0.0003 0.0018 0.0014
Bud Neck 0.0005 0.0021 0.0057 0.0012 0.0019 0.0066 0.0061 0.0012 0.0087 0.0007 0.0092 0.0026 0.0046 0.0008 0.0023 0.0005 0.0068 0.008
Bud Periphery 0.0008 0.0024 0.0048 0.0009 0.001 0.0021 0.0093 0.0066 0.0064 0.0005 0.0276 0.004 0.0004 0.0008 0.0012 0.0008 0.0028 0.0019
Bud Site 0.0014 0.019 0.0166 0.0003 0.0006 0.0006 0.0093 0.052 0.0192 0.0008 0.0266 0.0037 0.0014 0.0251 0.0141 0.0009 0.0082 0.0016
Cell Periphery 0.0041 0.01 0.0079 0.0016 0.0063 0.0028 0.0059 0.0087 0.0124 0.0027 0.0046 0.0047 0.0033 0.0085 0.0099 0.0034 0.0012 0.0045
Cytoplasm 0.0798 0.173 0.102 0.0803 0.1701 0.1629 0.1142 0.1726 0.1168 0.1142 0.0686 0.1881 0.1224 0.2279 0.1921 0.1193 0.1112 0.2204
Cytoplasmic Foci 0.0094 0.0037 0.0054 0.025 0.0035 0.0077 0.0108 0.008 0.0074 0.0033 0.004 0.0043 0.0199 0.0107 0.0071 0.011 0.0237 0.0196
Eisosomes 0.002 0.0011 0.0009 0.0003 0.0007 0.0005 0.0016 0.0009 0.0013 0.0008 0.0013 0.0004 0.0014 0.0006 0.0007 0.0003 0.0003 0.0003
Endoplasmic Reticulum 0.0014 0.0017 0.0012 0.0008 0.0012 0.0013 0.0068 0.0021 0.0016 0.0027 0.0023 0.0014 0.0033 0.0017 0.0019 0.0017 0.0015 0.0009
Endosome 0.0042 0.0008 0.0005 0.0007 0.002 0.0013 0.0066 0.001 0.0011 0.0004 0.0027 0.0008 0.0045 0.0004 0.0016 0.0009 0.0096 0.0005
Golgi 0.0015 0.0003 0.0003 0.0002 0.0014 0.0003 0.0034 0.0005 0.0008 0.0001 0.001 0.0005 0.0015 0.0002 0.0005 0.0002 0.0018 0.0001
Lipid Particles 0.0024 0.0006 0.0004 0.0002 0.0009 0.0007 0.0042 0.0017 0.0014 0.0007 0.0015 0.0007 0.008 0.0006 0.0009 0.001 0.0052 0.0006
Mitochondria 0.0019 0.0007 0.0007 0.0001 0.0004 0.0005 0.002 0.0007 0.0007 0.0003 0.0014 0.0004 0.0017 0.0003 0.0004 0.0004 0.0008 0.0002
None 0.8701 0.7691 0.8432 0.8862 0.7872 0.7987 0.7495 0.7302 0.7921 0.8596 0.8053 0.7795 0.7976 0.7147 0.748 0.8516 0.7715 0.7314
Nuclear Periphery 0.0023 0.0004 0.0003 0.0001 0.0036 0.0013 0.0051 0.0004 0.0006 0.0003 0.0033 0.0004 0.0016 0.0002 0.0011 0.0006 0.0067 0.0002
Nucleolus 0.0004 0.0008 0.0005 0.0001 0.0003 0.0003 0.0014 0.0008 0.0012 0.0018 0.0014 0.0003 0.0007 0.0006 0.0009 0.0006 0.0014 0.0004
Nucleus 0.001 0.0021 0.0009 0.0002 0.0006 0.0017 0.0034 0.0014 0.0011 0.0017 0.0025 0.0005 0.0014 0.0007 0.0082 0.0011 0.002 0.0009
Peroxisomes 0.0045 0.0002 0.0007 0.0003 0.0004 0.0007 0.0024 0.0012 0.0056 0.0001 0.0009 0.0005 0.0082 0.0001 0.0002 0.0002 0.0062 0.0002
Punctate Nuclear 0.003 0.0023 0.0008 0.0003 0.0021 0.0016 0.0025 0.0016 0.0026 0.0073 0.0043 0.001 0.003 0.0035 0.0046 0.0038 0.0286 0.0035
Vacuole 0.0006 0.0007 0.0009 0.0002 0.0004 0.0007 0.0016 0.0011 0.0012 0.0008 0.0014 0.0008 0.0009 0.0007 0.001 0.001 0.001 0.0007
Vacuole Periphery 0.0003 0.0001 0.0001 0 0.0002 0.0002 0.0006 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0 0.0001 0.0001 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.8196 3.1214 14.6696 21.0183 6.0363 12.7961 5.5232 16.3311 15.1962 7.4875
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1574 1776 1243 1622 1466 1419 3096 1765 3040 3195 4339 3387

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 655.26 703.47 867.14 896.48 649.35 648.45 825.12 917.41 652.41 679.03 837.16 907.39
Standard Deviation 67.68 90.53 100.38 123.33 87.28 81.22 105.18 126.69 77.81 90.74 105.55 125.53
Intensity Change Log 2 — 0.102421 0.404197 0.452204 — -0.002001 0.345608 0.498570 — 0.051391 0.375333 0.475468

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000250 0.001214 0.001519 0.043618 0.000550 0.001760 0.011596 0.037509 0.000394 0.001457 0.008709 0.040435
Bud Neck 0.026805 0.051646 0.007779 0.041279 0.028953 0.085282 0.048667 0.046339 0.027841 0.066585 0.036954 0.043916
Bud Site 0.004966 0.025749 0.021924 0.139453* 0.011279 0.025830 0.052615 0.141327* 0.008011 0.025785 0.043823 0.140430*
Cell Periphery 0.000383 0.000537 0.002787 0.076325 0.000362 0.000665 0.020989 0.094721 0.000373 0.000594 0.015775 0.085912
Cytoplasm 0.488196* 0.415466* 0.500535* 0.348879* 0.460971* 0.300022* 0.465020* 0.388700* 0.475067* 0.364194* 0.475194* 0.369630*
Cytoplasmic Foci 0.229103* 0.268863* 0.065655 0.015754 0.255999* 0.322089* 0.012341 0.013810 0.242073* 0.292503* 0.027614 0.014741
Eisosomes 0.000931 0.000911 0.000598 0.039928 0.000838 0.001682 0.009666 0.040497 0.000886 0.001253 0.007068 0.040225
Endoplasmic Reticulum 0.000683 0.001125 0.008425 0.009745 0.001212 0.000858 0.004755 0.005114 0.000938 0.001007 0.005806 0.007332
Endosome 0.008873 0.015134 0.004139 0.001249 0.016134 0.033072 0.000756 0.002129 0.012375 0.023101 0.001725 0.001708
Golgi 0.005623 0.009693 0.000305 0.001696 0.008014 0.023078 0.000410 0.001757 0.006776 0.015638 0.000380 0.001728
Lipid Particles 0.011201 0.009217 0.005432 0.002469 0.015001 0.019317 0.001587 0.001855 0.013034 0.013702 0.002688 0.002149
Mitochondria 0.004124 0.013122 0.000206 0.000745 0.007844 0.036839 0.001020 0.002756 0.005918 0.023655 0.000787 0.001793
Mitotic Spindle 0.001739 0.002841 0.000425 0.002814 0.001230 0.002304 0.000818 0.004354 0.001493 0.002602 0.000706 0.003616
None 0.004117 0.003014 0.003098 0.000778 0.006442 0.001972 0.001092 0.000621 0.005238 0.002551 0.001667 0.000696
Nuclear Periphery 0.000226 0.000182 0.000668 0.000123 0.000225 0.000186 0.000172 0.000105 0.000225 0.000184 0.000314 0.000113
Nuclear Periphery Foci 0.000246 0.000274 0.004317 0.001013 0.000436 0.000450 0.000355 0.000587 0.000338 0.000352 0.001490 0.000791
Nucleolus 0.000804 0.000829 0.000323 0.000050 0.001482 0.000922 0.000094 0.000066 0.001131 0.000871 0.000159 0.000059
Nucleus 0.170970* 0.114476 0.273675* 0.131847* 0.127668 0.054205 0.301541* 0.117505 0.150088* 0.087708 0.293558* 0.124373
Peroxisomes 0.006461 0.019390 0.000441 0.001065 0.009188 0.042812 0.000830 0.002069 0.007776 0.029793 0.000719 0.001588
Vacuole 0.033704 0.045042 0.097632 0.140899 0.044880 0.043775 0.065440 0.097463 0.039094 0.044479 0.074662 0.118264
Vacuole Periphery 0.000595 0.001274 0.000118 0.000272 0.001291 0.002879 0.000233 0.000716 0.000931 0.001987 0.000200 0.000503

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.73 -5.34 -19.26 -18.75 -18.56 -6.00 -13.41 -18.40 -17.73 -11.95 -8.13 -14.01 -26.63 -25.82* -19.48
Bud Neck -7.79 8.76 -4.57 4.13 -15.49 -13.09 -7.28 -6.49 9.38 0.99 -14.82 -4.35 -7.85 9.50 -3.25
Bud Site -10.70 -7.81 -25.10 -19.95 -20.42 -7.00 -15.70 -24.35 -21.05 -15.56 -12.41 -18.75 -34.98 -28.95 -23.17
Cell Periphery -2.35 -7.15 -20.56 -20.51 -19.81 -4.51 -17.85 -23.31 -23.23 -17.49 -4.70 -18.33 -30.98 -30.90* -24.16
Cytoplasm 8.54 0.61 17.31 8.31 14.55 17.24 -0.10 8.35 -9.74 10.04 17.35 1.31 17.89 -0.36 17.78
Cytoplasmic Foci -5.58 30.29 43.63 47.33 17.91 -8.18 45.81 45.31 50.44 -1.20 -9.38 58.33 62.80 68.60 10.98
Eisosomes 0.18 3.27 -18.59 -18.60 -18.77 -5.07 -16.94 -20.93 -20.41 -15.71 -3.72 -16.31 -27.96 -27.65 -22.69
Endoplasmic Reticulum -1.62 -10.50 -15.37 -13.40 -0.95 1.43 -8.79 -10.70 -12.04 -0.48 -0.38 -14.03 -18.76 -17.29 -2.74
Endosome -5.19 5.37 9.09 15.75 9.19 -7.48 12.97 11.31 15.87 -3.24 -8.72 14.74 14.34 21.15 0.38
Golgi -4.46 8.79 4.97 9.34 -2.29 -8.56 10.24 6.96 12.77 -2.44 -9.41 13.42 8.45 15.69 -3.29
Lipid Particles 1.88 4.58 9.72 11.44 3.21 -2.48 10.80 10.69 13.60 -1.20 -0.71 12.86 14.43 17.40 1.71
Mitochondria -6.90 8.17 6.73 10.20 -2.77 -10.00 7.36 4.43 12.04 -2.29 -11.83 10.00 6.58 15.04 -2.44
Mitotic Spindle -0.99 1.77 -0.76 0.14 -2.32 -1.16 0.73 -2.41 -1.39 -2.96 -1.51 1.63 -2.26 -0.92 -3.63
None 1.99 1.76 6.28 4.90 3.70 3.80 5.10 5.56 2.81 3.28 4.24 6.23 7.99 5.67 4.33
Nuclear Periphery 1.26 -11.23 2.69 1.73 16.77 1.28 1.83 5.60 3.64 6.05 1.79 -5.16 5.38 3.86 15.50
Nuclear Periphery Foci -0.52 -13.69 -12.13 -11.27 10.78 -0.11 0.65 -1.84 -0.88 -5.28 -0.19 -11.64 -8.93 -5.79 7.07
Nucleolus 0.36 3.37 5.09 9.69 7.37 1.66 4.41 4.49 6.79 2.75 1.67 5.79 6.35 11.24 7.83
Nucleus 10.40 -14.04 6.27 -3.81 18.88 16.58 -31.07 1.66 -14.48 32.40 17.17 -32.13 5.99 -10.85 37.60
Peroxisomes -9.43 10.06 8.81 14.81 -3.45 -13.26 9.88 7.42 16.78 -2.44 -15.96 13.73 10.89 21.68 -3.06
Vacuole -5.64 -18.50 -25.44 -22.70 -8.13 0.39 -10.18 -15.70 -15.38 -9.00 -3.22 -20.90 -29.42 -27.06 -14.54
Vacuole Periphery -7.24 8.81 3.02 8.11 -1.35 -4.76 7.45 1.87 5.05 -1.48 -6.65 8.52 2.58 6.88 -1.63
ORF
Human Ortholog
Description Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; SWAT-GFP fusion protein localizes to the cell periphery, while mCherry fusion localizes to both the cell periphery and vacuole; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History)
Localization
Cell Percentages cytoplasm (56%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

YHL026C

YHL026C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhl026c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available