Standard name
Human Ortholog
Description Endoplasmic reticulum (ER) membrane protein; involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.8 0.49 0.7 0.84 0.68 0.5 0.41 0.32 0.33 0.31 0.38 0.38 0.96 0.98 0.99 – – 0.57 0.15 0.05 0.07 0.28 0.3 0.33
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.06 0 0.06 0.06 0.06 0.06 0 0 0 – – 0 0 0 0 0.07 0.05 0.06
Nucleus 0 0.11 0.05 0 0 0.14 0.19 0.25 0.23 0.2 0.15 0.15 0 0 0 – – 0.15 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.27 0.53 0.37 0.19 0.4 0.48 0.54 0.64 0.59 0.64 0.62 0.65 0 0 0 – – 0.47 0.74 0.84 0.81 0.56 0.55 0.49
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 – – 0 1 2 1 0 2 3
Bud 0 2 1 5 1 4 1 3 0 1 1 0 0 0 0 – – 0 1 5 5 0 4 6
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 6 11 12 – – 0 2 3 2 0 0 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 5 3 3 – – 0 0 0 0 0 0 0
Cytoplasm 197 158 173 182 154 175 151 161 119 98 120 164 689 780 883 – – 120 42 19 17 63 93 117
Endoplasmic Reticulum 3 2 2 0 3 1 0 3 5 0 0 0 3 2 2 – – 14 1 1 4 3 4 1
Endosome 1 5 9 5 9 8 6 5 6 1 2 1 1 1 2 – – 5 9 11 7 4 8 7
Golgi 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 – – 2 2 4 0 0 2 2
Mitochondria 11 8 11 9 7 26 23 22 21 20 18 26 1 0 0 – – 7 8 5 3 15 16 20
Nucleus 5 35 13 3 10 51 69 128 81 63 47 62 3 3 1 – – 32 1 0 0 0 0 0
Nuclear Periphery 1 2 0 0 0 3 1 0 2 7 1 6 0 0 0 – – 1 1 0 1 0 0 0
Nucleolus 0 0 1 0 0 4 1 4 4 3 3 4 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 – – 0 0 0 0 0 1 0
SpindlePole 1 1 1 0 0 0 1 1 0 0 1 2 1 0 0 – – 0 0 0 1 2 1 6
Vac/Vac Membrane 66 170 90 40 90 171 197 322 211 204 195 277 26 9 5 – – 99 214 307 203 127 172 171
Unique Cell Count 247 323 246 216 225 353 367 507 357 318 314 427 716 796 895 210 290 366 251 225 315 350
Labelled Cell Count 287 383 301 245 274 444 450 649 449 399 388 542 736 810 909 280 290 366 251 225 315 350


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.3 4.6 4.8 4.8 5.4 6.1 7.1 7.4 7.9 7.9 8.3 5.1 5.0 5.1 4.6 4.9 5.8 5.1 5.3 5.3
Std Deviation (1e-4) 0.7 1.1 1.6 2.1 1.2 1.8 2.1 2.1 2.3 3.4 2.8 3.0 0.9 1.1 0.9 1.0 1.2 1.6 1.0 1.7 1.4
Intensity Change (Log2) – – – 0.05 0.04 0.23 0.4 0.62 0.68 0.77 0.77 0.84 0.13 0.11 0.15 -0.02 0.07 0.33 0.14 0.2 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Cytoplasm 3.5 -0.4 -5.1 -7.1 -10.0 -8.9 -9.3 -7.6 -8.0 11.5 13.6 15.0 – – -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – 3.4
Endosome 0 0 -1.0 -1.6 0 -1.5 0 0 0 0 0 0 – – -0.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Mitochondria 0 0 1.4 1.0 -0.1 0.8 0.9 0.7 0.9 0 0 0 – – -0.6
Nucleus -2.3 -0.4 3.6 4.8 6.6 5.8 5.0 3.7 3.7 0 0 0 – – 3.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Vacuole -4.3 0.8 2.9 4.2 7.0 5.4 6.5 6.0 7.1 -13.7 -16.6 -18.1 – – 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9007 0.5809 0.2112 1.3763 0.4574 0.9556 2.0323 1.9058 1.6461 3.0737 2.4107 2.2738 1.4554 1.2121 1.6902 1.9632 2.3625 1.6327
Actin 0.0681 0.0003 0.0132 0.0386 0.0282 0.004 0.0089 0.0003 0.0004 0.0162 0.0141 0.0002 0.031 0.0002 0.0003 0.0004 0.0001 0.0001
Bud 0.0011 0.0021 0.0019 0.0007 0.0022 0.0004 0.0007 0.0023 0.0003 0.0006 0.0009 0.0005 0.0009 0.001 0.0015 0.0009 0.0002 0.0002
Bud Neck 0.0127 0.0012 0.0008 0.0005 0.0016 0.0073 0.0063 0.0004 0.0002 0.0006 0.0006 0.0007 0.0122 0.0001 0.0002 0.0002 0.0004 0.0004
Bud Periphery 0.0019 0.002 0.0033 0.0014 0.0031 0.0006 0.0016 0.0021 0.0004 0.0008 0.001 0.0012 0.0023 0.0014 0.0042 0.0016 0.0001 0.0005
Bud Site 0.0091 0.0068 0.0018 0.0009 0.0028 0.0007 0.0157 0.0228 0.0003 0.0023 0.0085 0.0001 0.0056 0.0065 0.0007 0.0002 0.0004 0.0006
Cell Periphery 0.001 0.0005 0.0004 0.0003 0.0005 0.0004 0.0021 0.0004 0.0002 0.0002 0.0003 0.0006 0.0009 0.0002 0.0004 0.0249 0.0001 0.0005
Cytoplasm 0.0709 0.1654 0.1802 0.2229 0.2539 0.2659 0.1747 0.138 0.1308 0.204 0.2136 0.1779 0.0976 0.1292 0.0934 0.2342 0.167 0.1799
Cytoplasmic Foci 0.0442 0.0475 0.0441 0.0325 0.069 0.09 0.0569 0.0175 0.0166 0.0341 0.0736 0.0374 0.0423 0.0141 0.0097 0.0144 0.0212 0.0264
Eisosomes 0.0009 0.0001 0.0002 0.0002 0.0003 0.0001 0.0007 0.0002 0.0003 0.0005 0.0002 0.0001 0.0008 0.0002 0.0002 0.0001 0.0001 0
Endoplasmic Reticulum 0.0071 0.0028 0.0054 0.0046 0.0057 0.0052 0.0044 0.0018 0.0015 0.0022 0.0037 0.0014 0.0093 0.0017 0.0013 0.0028 0.0006 0.0064
Endosome 0.1535 0.0796 0.0706 0.1389 0.1933 0.1218 0.0443 0.0285 0.026 0.1443 0.1205 0.0501 0.0687 0.0281 0.0221 0.0325 0.0879 0.0883
Golgi 0.0095 0.0017 0.0037 0.0031 0.0048 0.0039 0.0026 0.0005 0.0056 0.0196 0.0042 0.0039 0.004 0.0003 0.0008 0.0007 0.001 0.0015
Lipid Particles 0.0284 0.0041 0.0078 0.0023 0.0164 0.0143 0.0092 0.0023 0.0094 0.031 0.0022 0.0075 0.0095 0.0032 0.0006 0.0029 0.0008 0.0016
Mitochondria 0.0069 0.0015 0.0045 0.0064 0.0038 0.0042 0.0026 0.0007 0.0013 0.0437 0.0022 0.0061 0.0024 0.0003 0.0029 0.0018 0.0003 0.0013
None 0.3511 0.5721 0.5953 0.4173 0.2504 0.3778 0.5778 0.7334 0.7846 0.4114 0.5185 0.68 0.636 0.7985 0.8101 0.6255 0.6828 0.6024
Nuclear Periphery 0.0287 0.0023 0.0088 0.0025 0.0025 0.0029 0.0212 0.0012 0.0006 0.0035 0.0014 0.0005 0.0141 0.0016 0.0009 0.0021 0.0003 0.0017
Nucleolus 0.0054 0.0026 0.0016 0.0005 0.0009 0.0014 0.0012 0.0006 0.0003 0.0004 0.0002 0.0005 0.0012 0.0003 0.0005 0.0003 0.0002 0.0003
Nucleus 0.0451 0.0094 0.0061 0.0029 0.0055 0.005 0.0089 0.0021 0.0007 0.0015 0.0012 0.0008 0.0063 0.0012 0.0013 0.0014 0.0006 0.0012
Peroxisomes 0.0147 0.0009 0.0021 0.0014 0.0108 0.0023 0.0061 0.0003 0.0042 0.0468 0.0015 0.0016 0.0123 0.0002 0.0003 0.0004 0.0002 0.0003
Punctate Nuclear 0.0111 0.01 0.004 0.0008 0.0027 0.0035 0.0017 0.0005 0.0004 0.001 0.001 0.0008 0.0019 0.0008 0.0003 0.0006 0.0002 0.0002
Vacuole 0.118 0.0825 0.0393 0.1076 0.1318 0.0817 0.0492 0.0418 0.0142 0.028 0.0263 0.0251 0.0365 0.0103 0.0437 0.0399 0.0335 0.0789
Vacuole Periphery 0.0106 0.0046 0.0052 0.0134 0.0098 0.0067 0.0032 0.0024 0.0018 0.0074 0.0044 0.0032 0.0041 0.0007 0.0048 0.012 0.0019 0.0073

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 0.6069 3.7979 1.2078 3.0808 0.3935 1.8198 1.6457 4.9985 3.4789 2.7105
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
113 1525 326 121 2343 1076 1995 1395 2456 2601 2321 1516

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 905.37 794.11 934.85 854.05 716.69 744.05 886.91 830.48 725.37 773.40 893.64 832.36
Standard Deviation 143.81 102.34 136.25 96.35 79.53 91.25 102.67 107.88 92.45 100.96 109.30 107.20
Intensity Change Log 2 — -0.189169 0.046227 -0.084187 — 0.054050 0.307438 0.212596 — -0.076622 0.167506 0.054519

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002493 0.000124 0.001965 0.001155 0.000095 0.000492 0.000353 0.004721 0.000206 0.000276 0.000579 0.004436
Bud Neck 0.004743 0.027548 0.018404 0.008399 0.039254 0.053822 0.046441 0.021683 0.037666 0.038417 0.042503 0.020623
Bud Site 0.064684 0.016761 0.029502 0.019335 0.008721 0.017115 0.025396 0.043496 0.011296 0.016908 0.025973 0.041567
Cell Periphery 0.000557 0.000222 0.000243 0.000420 0.000163 0.000216 0.000170 0.000260 0.000181 0.000219 0.000181 0.000273
Cytoplasm 0.229951* 0.153285* 0.096636 0.240300* 0.296497* 0.239790* 0.158597* 0.389222* 0.293436* 0.189071* 0.149894* 0.377336*
Cytoplasmic Foci 0.092280 0.077034 0.044354 0.068801 0.121711 0.234013* 0.043352 0.060682 0.120357 0.141974 0.043493 0.061330
Eisosomes 0.000121 0.000062 0.000065 0.000173 0.000159 0.000305 0.000046 0.000111 0.000157 0.000162 0.000049 0.000116
Endoplasmic Reticulum 0.002025 0.001567 0.005054 0.004352 0.001076 0.000940 0.003538 0.002542 0.001120 0.001307 0.003751 0.002687
Endosome 0.043169 0.084496 0.108340 0.035665 0.070978 0.091789 0.138072 0.038771 0.069698 0.087513 0.133896 0.038523
Golgi 0.025341 0.002599 0.003476 0.000475 0.001923 0.006867 0.001073 0.004485 0.003001 0.004365 0.001411 0.004165
Lipid Particles 0.002915 0.002777 0.003684 0.004102 0.006927 0.008864 0.004969 0.004066 0.006742 0.005295 0.004788 0.004069
Mitochondria 0.002209 0.001769 0.000246 0.000644 0.004295 0.009024 0.001093 0.002554 0.004199 0.004770 0.000974 0.002402
Mitotic Spindle 0.001223 0.002307 0.004427 0.013890 0.001112 0.004997 0.007229 0.040159 0.001117 0.003420 0.006836 0.038063
None 0.000269 0.000981 0.000263 0.001551 0.000604 0.000672 0.000639 0.001815 0.000589 0.000853 0.000586 0.001794
Nuclear Periphery 0.000469 0.000208 0.001140 0.000786 0.000260 0.000339 0.001766 0.001028 0.000270 0.000262 0.001678 0.001008
Nuclear Periphery Foci 0.001036 0.000244 0.005212 0.004005 0.000163 0.000183 0.002585 0.002405 0.000203 0.000219 0.002954 0.002533
Nucleolus 0.000329 0.000852 0.003204 0.000264 0.000672 0.000783 0.003671 0.000927 0.000656 0.000823 0.003606 0.000874
Nucleus 0.071015 0.019372 0.055196 0.109763 0.052870 0.041337 0.090939 0.099638 0.053705 0.028458 0.085918 0.100446
Peroxisomes 0.002943 0.004511 0.004440 0.003722 0.005019 0.022593 0.007742 0.010145 0.004923 0.011991 0.007279 0.009632
Vacuole 0.450127* 0.593180* 0.603416* 0.481941* 0.384518* 0.261720* 0.459381* 0.268564* 0.387536* 0.456059* 0.479612* 0.285595*
Vacuole Periphery 0.002101 0.010102 0.010735 0.000257 0.002982 0.004139 0.002944 0.002724 0.002941 0.007635 0.004039 0.002527

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.36 -0.15 1.29 -2.96 1.11 -3.91 -3.31 -2.67 -2.18 -2.38 -2.15 -2.60 -2.64 -2.50 -2.14
Bud Neck -13.31 -6.52 -0.98 9.85 4.72 -3.99 -1.61 10.72 11.74 11.49 -0.26 -0.94 10.90 12.15 11.60
Bud Site 2.11 1.16 2.48 1.70 3.37 -4.58 -7.26 -6.93 -2.94 -0.91 -4.76 -7.00 -6.01 -3.13 -0.28
Cell Periphery 0.98 0.88 0.54 -0.72 -0.53 -2.30 0.90 -0.52 1.87 -1.44 -2.14 0.73 -0.44 1.77 -1.24
Cytoplasm 2.85 4.06 0.18 -2.77 -4.04 6.30 14.45 -7.03 -11.82 -19.10 14.41 16.09 -6.41 -18.63 -20.01
Cytoplasmic Foci -0.53 3.54 2.75 4.28 -0.21 -15.79 22.50 21.00 28.59 -0.28 -4.80 22.97 21.19 24.12 -0.30
Eisosomes 4.38 3.07 -1.58 -4.71 -3.87 -6.76 15.22 7.06 10.00 -6.49 -0.47 15.01 6.72 6.07 -6.92
Endoplasmic Reticulum 1.20 -4.13 -3.78 -5.37 0.38 0.97 -12.07 -4.46 -4.75 3.39 -1.40 -13.22 -5.32 -4.61 3.69
Endosome -8.08 -5.95 1.44 9.24 6.96 -4.38 -7.28 15.68 16.24 18.26 -5.16 -7.80 15.81 21.38 19.55
Golgi 1.64 1.60 1.95 6.23 2.20 -7.02 4.06 -0.15 5.00 -1.71 -3.32 3.08 0.84 3.35 -1.18
Lipid Particles -0.25 -1.78 -1.33 -1.31 0.52 -2.43 4.67 6.66 7.13 3.13 2.66 4.80 6.66 4.16 3.12
Mitochondria -0.44 1.21 1.11 5.32 -0.36 -3.67 4.42 3.52 6.70 -1.27 -0.72 4.62 3.65 6.03 -1.45
Mitotic Spindle -1.58 -2.02 -0.89 -0.61 0.18 -2.33 -4.93 -7.51 -5.76 -4.86 -2.42 -5.23 -7.54 -6.64 -4.85
None -0.84 0.50 -1.97 -1.17 -2.09 -0.24 -1.66 -5.79 -4.50 -5.24 -0.62 -1.29 -6.07 -3.11 -5.74
Nuclear Periphery 5.37 -2.78 -4.38 -7.68 -1.10 -1.35 -9.81 -17.80 -14.48 2.45 0.41 -10.36 -18.48 -18.73 2.13
Nuclear Periphery Foci 4.93 -6.37 -4.02 -5.37 1.74 -0.64 -8.07 -4.60 -4.55 1.71 -0.40 -10.13 -5.30 -5.28 2.43
Nucleolus -4.31 -4.89 -0.41 3.33 4.73 -1.12 -8.64 -0.60 0.17 7.94 -1.69 -9.34 -0.53 0.58 8.59
Nucleus 4.81 1.31 -2.20 -8.08 -4.20 3.68 -14.29 -19.07 -20.29 -5.85 11.35 -13.51 -19.25 -25.35 -7.17
Peroxisomes -3.65 -2.55 -0.01 2.13 1.65 -8.28 -1.54 -0.12 8.00 1.21 -6.82 -1.47 0.01 6.23 1.26
Vacuole -3.17 -2.56 -0.78 2.20 1.66 11.96 -6.52 5.44 -6.07 11.12 -7.18 -8.40 4.47 10.79 11.95
Vacuole Periphery -10.84 -6.16 2.04 17.87 8.17 -3.74 2.85 4.51 6.44 2.63 -12.54 -1.27 4.90 13.59 5.25
Standard name
Human Ortholog
Description Endoplasmic reticulum (ER) membrane protein; involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3
Localization
Cell Percentages vacuole (59%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Wsc4

Wsc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Wsc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available