Standard name
Human Ortholog
Description Glycerol kinase; converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.11 0.05 0 0 0 0 0 0
Cytoplasm 0.93 0.98 0.98 0.99 0.97 0.96 0.91 0.93 0.85 0.79 0.76 0.75 0.97 0.97 0.99 0.85 0.83 0.83 0.64 0.82 0.71 0.67 0.72 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.05 0.1 0 0.25 0.35 0.35 0.29 0 0 0 0.05 0.07 0.05 0.17 0.11 0.17 0.19 0.13 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 3 2 1 0 0 0 0 0 0 0 0 2 2 2 3 1 5 2 4 2
Bud 0 0 1 1 1 2 5 1 5 8 12 7 0 1 0 0 0 0 1 0 0 2 5 3
Bud Neck 1 1 15 6 24 18 18 14 9 6 2 11 0 1 0 0 1 1 1 1 2 3 1 1
Bud Site 0 0 0 0 0 0 0 0 1 2 5 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 2 11 7 2 1 2 4 1 2 2 1 1 1 0 17 14 6 2 0 7 2 1 2
Cytoplasm 187 179 361 287 350 481 530 372 327 247 227 205 213 268 220 127 110 104 163 155 241 101 165 156
Endoplasmic Reticulum 1 3 1 0 1 0 2 2 0 0 0 0 3 3 1 9 4 3 10 0 1 1 3 2
Endosome 0 0 0 0 0 0 1 0 2 0 1 0 0 0 0 3 6 7 10 2 1 0 0 1
Golgi 0 0 1 0 1 0 0 1 0 0 0 0 0 1 0 3 0 0 1 0 0 0 0 0
Mitochondria 12 7 0 4 3 24 60 17 96 108 104 79 7 1 1 7 9 6 44 20 56 29 29 26
Nucleus 1 1 0 1 0 4 3 7 2 0 5 4 0 0 1 1 2 3 0 0 0 0 0 0
Nuclear Periphery 2 1 0 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 3 2 16 3 6 8 3 6 0 0 0 1 2 3 2 0 2 1 3 2
Vac/Vac Membrane 0 2 0 2 0 2 2 2 2 1 2 2 1 0 0 12 1 6 6 2 1 4 7 11
Unique Cell Count 202 183 368 291 360 500 581 400 384 313 297 275 219 275 222 149 133 125 258 189 338 152 228 220
Labelled Cell Count 210 196 392 308 388 536 641 425 451 382 363 316 225 278 223 182 151 141 258 189 338 152 228 220


Mitochondria, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 3.7 4.4 4.2 4.5 3.6 3.8 4.2 3.3 3.2 3.1 3.5 3.8 4.1 4.4 6.7 6.5 6.8 3.8 4.0 4.3
Std Deviation (1e-4) 0.4 0.8 0.7 1.1 1.3 1.0 1.1 1.0 1.1 0.9 0.8 0.8 1.0 1.0 1.9 1.7 1.5 2.0 1.7 1.9 1.6
Intensity Change (Log2) – – – -0.08 0.05 -0.28 -0.21 -0.08 -0.41 -0.47 -0.5 -0.34 -0.2 -0.11 0.02 0.6 0.56 0.62 -0.21 -0.14 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8955 -0.8869 -0.9641 -1.175 -1.132 -1.1388 -0.3212 -0.3095 -0.3627 -0.0112 0.0281 -0.3371 -0.9508 -0.9561 -1.0489 -0.793 -0.9227 -0.9562
Actin 0.027 0.0025 0.0035 0.0058 0.0024 0.0023 0.0432 0.0013 0.0085 0.0552 0.0286 0.0058 0.0295 0.0011 0.0027 0.0037 0.0528 0.0062
Bud 0.0003 0.0036 0.0018 0.0012 0.0009 0.0003 0.0016 0.0041 0.0013 0.004 0.0003 0.0014 0.0005 0.0004 0.0004 0.0004 0.0006 0.0003
Bud Neck 0.0023 0.0002 0.0074 0.0033 0.0001 0.0006 0.0088 0.0003 0.0007 0.0039 0.0005 0.0013 0.001 0.0002 0.0012 0.0007 0.0002 0.0003
Bud Periphery 0.0005 0.0032 0.0068 0.0071 0.0034 0.0012 0.003 0.0035 0.001 0.0057 0.0008 0.0013 0.0008 0.0009 0.001 0.0005 0.0009 0.0006
Bud Site 0.001 0.0097 0.0066 0.0008 0.0004 0.0001 0.0082 0.0132 0.0017 0.0424 0.0007 0.0003 0.0056 0.0019 0.0006 0.0054 0.0013 0.0002
Cell Periphery 0.0002 0.0005 0.0002 0.0006 0.0001 0 0.0005 0.0002 0.0001 0.001 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0415 0.0665 0.0513 0.068 0.0559 0.0737 0.1396 0.16 0.0985 0.0979 0.1529 0.201 0.0687 0.0929 0.0824 0.0819 0.1286 0.1578
Cytoplasmic Foci 0.0193 0.0077 0.0186 0.0108 0.019 0.011 0.0212 0.0122 0.017 0.0391 0.0179 0.0162 0.0361 0.0143 0.0077 0.0232 0.0084 0.0214
Eisosomes 0.0005 0.0002 0.0003 0.0005 0.0001 0.0001 0.0007 0.0002 0.0002 0.0004 0.0004 0.0001 0.001 0.0003 0.0005 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0069 0.0009 0.0015 0.0026 0.0023 0.0003 0.005 0.0013 0.001 0.0003 0.0046 0.001 0.0031 0.0008 0.001 0.0005 0.0013 0.0016
Endosome 0.0123 0.0006 0.0077 0.0042 0.0289 0.002 0.0069 0.0031 0.0034 0.0183 0.0268 0.0038 0.0139 0.0012 0.0028 0.0408 0.0118 0.0143
Golgi 0.0044 0.0001 0.0033 0.0007 0.0044 0.0016 0.0048 0.0003 0.0018 0.0037 0.0082 0.0021 0.0042 0.0004 0.0014 0.007 0.004 0.0059
Lipid Particles 0.0137 0.0005 0.0051 0.0017 0.0191 0.0015 0.0084 0.0005 0.0032 0.0047 0.0398 0.0013 0.0126 0.0015 0.003 0.0043 0.0011 0.0105
Mitochondria 0.0015 0.0005 0.0039 0.0027 0.023 0.0009 0.0048 0.0007 0.0083 0.0129 0.0333 0.0032 0.005 0.001 0.0114 0.055 0.0016 0.006
None 0.8179 0.8968 0.8681 0.8746 0.8156 0.9011 0.7091 0.7859 0.838 0.664 0.6431 0.7497 0.7691 0.8757 0.8738 0.7499 0.7631 0.7605
Nuclear Periphery 0.0166 0.0007 0.0014 0.0029 0.0056 0.0003 0.0084 0.0011 0.0009 0.0003 0.0099 0.0008 0.0115 0.0003 0.0016 0.0003 0.0085 0.0033
Nucleolus 0.0026 0.0011 0.0005 0.0013 0.001 0.0001 0.0039 0.0005 0.0005 0.0055 0.0011 0.0003 0.0016 0.0002 0.0004 0.0004 0.0003 0.0003
Nucleus 0.0064 0.0019 0.0025 0.0023 0.0013 0.0002 0.0066 0.0023 0.0011 0.0026 0.0027 0.0017 0.0047 0.0005 0.001 0.0008 0.005 0.0007
Peroxisomes 0.0107 0.0002 0.0047 0.0011 0.005 0.0016 0.0082 0.0025 0.0097 0.0326 0.0188 0.0031 0.02 0.0047 0.004 0.0199 0.0031 0.0046
Punctate Nuclear 0.0125 0.0014 0.0039 0.0052 0.0061 0.0006 0.0053 0.0056 0.0021 0.0022 0.0045 0.0047 0.0066 0.001 0.0019 0.0027 0.0008 0.0036
Vacuole 0.0013 0.0007 0.0005 0.0019 0.0027 0.0003 0.0014 0.0009 0.0009 0.0026 0.0027 0.0007 0.002 0.0005 0.0006 0.0017 0.0045 0.0012
Vacuole Periphery 0.0007 0.0001 0.0003 0.0007 0.0026 0.0001 0.0006 0.0001 0.0002 0.0005 0.0023 0.0003 0.0023 0.0001 0.0003 0.0007 0.0017 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.5836 10.651 10.6526 11.8678 14.7763 8.8016 11.2375 13.2729 15.5063 13.0269
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
285 1837 81 1319 1821 1205 127 1273 2106 3042 208 2592

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 623.98 766.30 945.96 832.43 648.31 723.12 879.86 799.20 645.02 749.20 905.60 816.11
Standard Deviation 57.48 102.52 111.05 113.03 88.26 207.01 103.27 149.34 85.16 154.17 111.14 133.16
Intensity Change Log 2 — 0.296410 0.600279 0.415829 — 0.157551 0.440590 0.301873 — 0.227323 0.521117 0.358886

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000157 0.000191 0.000437 0.002721 0.000127 0.000646 0.000254 0.000338 0.000131 0.000371 0.000325 0.001551
Bud Neck 0.040167 0.016802 0.001481 0.011898 0.019968 0.050323 0.001887 0.008776 0.022701 0.030080 0.001729 0.010365
Bud Site 0.014536 0.008083 0.002448 0.048603 0.005057 0.015621 0.002940 0.026771 0.006340 0.011069 0.002749 0.037881
Cell Periphery 0.000370 0.000106 0.000324 0.000226 0.000117 0.000213 0.000192 0.000217 0.000151 0.000149 0.000243 0.000222
Cytoplasm 0.381079* 0.522148* 0.260280* 0.429205* 0.463681* 0.275009* 0.305255* 0.413841* 0.452502* 0.424251* 0.287741* 0.421659*
Cytoplasmic Foci 0.320832* 0.185354* 0.051184 0.027965 0.221709* 0.347606* 0.036988 0.027762 0.235123* 0.249625* 0.042516 0.027865
Eisosomes 0.000523 0.000080 0.000159 0.000063 0.000176 0.000291 0.000113 0.000047 0.000223 0.000164 0.000131 0.000055
Endoplasmic Reticulum 0.000990 0.000514 0.005270 0.002189 0.001049 0.000594 0.003301 0.002062 0.001041 0.000545 0.004068 0.002127
Endosome 0.026117 0.013726 0.008920 0.009935 0.014270 0.052026 0.012438 0.005768 0.015873 0.028898 0.011068 0.007888
Golgi 0.006178 0.002104 0.000173 0.006367 0.002059 0.014299 0.000581 0.002138 0.002617 0.006934 0.000422 0.004290
Lipid Particles 0.020819 0.003348 0.006255 0.001593 0.012323 0.013666 0.004165 0.001312 0.013473 0.007435 0.004979 0.001455
Mitochondria 0.023931 0.004239 0.000313 0.005354 0.004835 0.029532 0.000963 0.002822 0.007419 0.014258 0.000710 0.004111
Mitotic Spindle 0.000341 0.007239 0.000345 0.049395 0.000788 0.007179 0.001327 0.030560 0.000728 0.007216 0.000944 0.040145
None 0.003099 0.002711 0.002223 0.007478 0.005725 0.001405 0.004134 0.007309 0.005369 0.002193 0.003390 0.007395
Nuclear Periphery 0.000743 0.000424 0.001597 0.001564 0.000722 0.000296 0.001485 0.001757 0.000725 0.000373 0.001528 0.001658
Nuclear Periphery Foci 0.000279 0.000396 0.002505 0.001325 0.000498 0.000246 0.001063 0.001478 0.000468 0.000337 0.001624 0.001400
Nucleolus 0.001349 0.001616 0.001282 0.000505 0.002468 0.001921 0.001337 0.000739 0.002316 0.001737 0.001315 0.000620
Nucleus 0.101591 0.164315* 0.528583* 0.252016* 0.182103* 0.079825 0.507039* 0.345861* 0.171207* 0.130847* 0.515429* 0.298106*
Peroxisomes 0.015799 0.007721 0.001071 0.003335 0.005158 0.035299 0.001105 0.000596 0.006598 0.018645 0.001092 0.001990
Vacuole 0.038265 0.057691 0.124759 0.134374 0.056016 0.070147 0.111293 0.112332 0.053614 0.062625 0.116537 0.123548
Vacuole Periphery 0.002835 0.001192 0.000391 0.003887 0.001153 0.003855 0.002142 0.007515 0.001380 0.002247 0.001460 0.005669

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.13 -2.63 -2.17 -2.10 -1.05 -3.34 -2.87 -4.16 2.19 -0.28 -3.67 -3.61 -2.56 -1.52 -1.28
Bud Neck 4.90 8.20 6.95 6.05 -5.47 -8.56 13.12 8.60 12.93 -4.78 -3.63 15.30 10.85 13.97 -7.48
Bud Site 2.01 3.49 -2.38 -5.76 -7.52 -5.97 1.93 -4.68 0.09 -5.82 -3.92 3.18 -7.22 -4.70 -9.56
Cell Periphery 3.12 0.12 2.69 -2.15 5.00 -6.40 -4.55 -0.96 3.47 2.38 0.21 -4.51 0.77 0.70 5.28
Cytoplasm -8.97 5.22 3.33 19.69 -3.74 19.62 8.39 12.45 -6.30 -3.21 3.63 11.15 15.10 11.90 -4.70
Cytoplasmic Foci 9.97 18.38 23.59 33.92 3.32 -15.25 29.14 40.35 47.77 2.44 -2.33 32.36 46.55 53.89 4.11
Eisosomes 4.38 3.28 4.48 2.07 6.19 -3.52 1.99 6.77 8.61 6.45 2.54 2.99 7.71 8.89 8.63
Endoplasmic Reticulum 1.19 -5.27 -6.28 -20.47 3.40 3.46 -5.22 -11.01 -14.63 2.47 4.51 -7.38 -15.49 -25.42 3.99
Endosome 3.12 3.75 5.57 7.61 3.91 -12.65 1.32 10.99 17.25 1.64 -7.73 2.43 11.70 18.24 2.56
Golgi 3.35 5.00 3.11 0.30 -2.41 -10.15 5.62 3.74 11.00 -1.22 -7.74 8.61 2.87 8.76 -2.59
Lipid Particles 4.57 3.46 4.99 8.08 6.12 -0.94 6.97 11.30 12.61 5.39 5.72 7.17 12.10 14.18 7.94
Mitochondria 3.37 4.05 3.73 2.46 -2.55 -9.45 6.82 5.18 10.89 -1.88 -4.71 7.26 5.62 10.77 -2.96
Mitotic Spindle -4.57 -0.37 -8.36 -5.74 -8.32 -3.70 -0.98 -7.63 -4.63 -7.38 -5.53 -0.65 -11.14 -7.37 -11.02
None 0.40 1.10 -1.32 -2.49 -3.00 4.15 1.98 0.21 -5.32 -2.17 3.51 2.61 0.26 -5.14 -3.32
Nuclear Periphery 0.93 -3.26 -5.90 -20.44 -3.13 3.21 -6.84 -10.26 -15.64 -2.22 2.88 -7.83 -13.75 -24.75 -3.80
Nuclear Periphery Foci -1.42 -5.93 -8.10 -7.97 3.22 3.85 -4.48 -4.34 -6.03 0.53 2.18 -6.91 -7.84 -9.61 2.68
Nucleolus -0.45 0.44 3.71 2.55 3.94 1.49 3.16 4.04 3.64 1.99 1.71 3.14 4.40 3.92 3.88
Nucleus -7.49 -12.25 -18.21 -14.89 6.66 17.86 -10.98 -20.27 -31.77 3.99 8.14 -15.23 -23.51 -31.33 7.69
Peroxisomes 2.76 5.18 5.08 7.54 -0.39 -14.05 7.88 10.06 16.65 1.01 -10.74 9.44 9.55 17.83 0.05
Vacuole -4.88 -6.43 -28.51 -27.63 -4.51 -3.71 -6.39 -20.40 -17.57 -0.68 -3.72 -8.60 -34.00 -32.27 -3.63
Vacuole Periphery 2.36 3.54 1.96 -0.06 -1.58 -5.27 -0.14 -2.11 1.09 -1.25 -3.24 0.72 -1.31 0.45 -1.42
Standard name
Human Ortholog
Description Glycerol kinase; converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
Localization
Cell Percentages cytoplasm (18%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gut1

Gut1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gut1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available