Standard name
Human Ortholog
Description Low affinity methionine permease; similar to Mup1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.31 0.19 0.25 0.22 0.32 0.44 0.53 0.57 0.65 0.72 0.7 0.45 0.45 0.7 0.11 0.06 0 0.09 0.16 0 0.15 0.15 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.3 0.1 0 0 0.12 0.12 0.14 0.19 0.19 0.23 0.22 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Nucleus 0.19 0.23 0.24 0.26 0.12 0.14 0.1 0.09 0.05 0 0 0 0.16 0.16 0.06 0.47 0.52 0.52 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.53 0.5 0.61 0.61 0.75 0.61 0.56 0.44 0.43 0.37 0.24 0.25 0.45 0.46 0.3 0.54 0.48 0.54 0.74 0.74 0.82 0.72 0.69 0.65
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4
Bud 0 1 1 2 0 1 4 13 8 10 7 7 0 0 0 0 0 0 1 2 3 7 21 20
Bud Neck 0 0 0 0 1 0 1 2 0 0 0 2 8 7 8 0 0 0 1 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 49 22 52 67 78 153 293 414 295 376 258 339 472 472 769 14 9 1 14 13 10 41 88 107
Endoplasmic Reticulum 0 1 0 0 0 1 0 0 0 0 1 0 1 1 1 2 3 0 0 0 0 1 1 2
Endosome 1 0 3 1 5 2 6 2 0 2 2 0 16 17 19 1 3 0 1 0 5 3 17 10
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 2 0 0 0
Mitochondria 15 21 26 8 15 59 80 112 99 109 83 104 9 9 2 7 9 0 6 2 9 6 12 16
Nucleus 28 16 65 72 42 65 68 73 24 22 9 7 163 163 71 60 84 25 6 2 11 7 5 3
Nuclear Periphery 2 3 9 13 14 3 5 9 11 12 2 12 3 3 1 2 3 1 0 0 2 0 5 2
Nucleolus 1 0 1 2 2 2 2 1 0 3 2 0 3 3 3 2 3 1 0 0 1 0 1 1
Peroxisomes 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 2 4 1 5 2 2 3 4 4 0 0 0 4 0 2 5 16 15
Vac/Vac Membrane 78 35 165 167 273 290 370 341 222 218 86 120 469 474 328 68 77 26 114 64 236 198 400 358
Unique Cell Count 147 70 271 272 362 478 663 783 518 582 359 483 1038 1038 1104 127 162 48 155 88 288 276 577 552
Labelled Cell Count 174 99 324 334 430 576 832 974 662 757 452 593 1148 1154 1206 156 192 54 155 88 288 276 577 552


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 4.6 6.8 8.1 8.2 6.7 5.7 5.0 4.6 4.4 4.1 4.2 6.2 6.2 5.4 10.8 11.1 15.8 6.4 6.5 6.2
Std Deviation (1e-4) 2.0 1.8 2.7 3.8 3.9 3.4 2.9 2.6 2.2 2.3 1.8 1.8 1.6 1.6 1.4 4.8 4.8 6.5 2.1 2.0 3.5
Intensity Change (Log2) – – – 0.26 0.27 -0.03 -0.25 -0.43 -0.55 -0.62 -0.72 -0.7 -0.13 -0.13 -0.32 0.67 0.71 1.22 -0.09 -0.07 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -1.4 -2.7 -0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9423 2.465 2.333 1.369 3.5319 2.5049 0.5862 3.4223 1.8697 2.2478 1.4536 2.6532 0.7093 2.3487 1.6604 1.7182 1.8289 1.7022
Actin 0.0101 0.0001 0.0002 0 0.0255 0.001 0.0078 0 0.0005 0.0099 0 0.0002 0.0131 0.0001 0.0059 0.0001 0.0001 0.0004
Bud 0.0012 0.0038 0.0079 0.0012 0.0005 0.0009 0.003 0.0013 0.0069 0.0008 0.0003 0.001 0.0008 0.0034 0.0062 0.002 0.0002 0.0009
Bud Neck 0.001 0.0009 0.0013 0.0009 0.0017 0.0012 0.0014 0.0005 0.0026 0.0009 0.0005 0.0031 0.0028 0.0011 0.001 0.0008 0.0005 0.0015
Bud Periphery 0.0027 0.0051 0.0161 0.0016 0.0009 0.0038 0.0086 0.0032 0.0093 0.0013 0.0009 0.0037 0.0021 0.0069 0.0085 0.0049 0.0005 0.0032
Bud Site 0.001 0.0092 0.0055 0.0009 0.0029 0.0005 0.0047 0.0007 0.008 0.0015 0.0003 0.0009 0.0018 0.0047 0.0057 0.001 0.0004 0.0006
Cell Periphery 0.0015 0.0025 0.0043 0.0013 0.0029 0.0014 0.0034 0.0056 0.0055 0.0028 0.0016 0.0035 0.0029 0.0048 0.0029 0.0016 0.0018 0.0029
Cytoplasm 0.0273 0.0331 0.0132 0.0145 0.0028 0.0305 0.014 0.0303 0.0206 0.0058 0.0072 0.009 0.0283 0.0529 0.0335 0.0354 0.0613 0.0396
Cytoplasmic Foci 0.024 0.017 0.0146 0.0093 0.0019 0.0108 0.0412 0.0154 0.0264 0.0224 0.0051 0.0222 0.04 0.0248 0.0221 0.0232 0.0194 0.0267
Eisosomes 0.0003 0 0 0 0.0003 0 0.0001 0 0.0001 0.0005 0 0 0.0001 0 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0072 0.0029 0.0035 0.0008 0.0052 0.003 0.0026 0.0043 0.0025 0.0009 0.0013 0.0015 0.0041 0.0065 0.0033 0.0029 0.0016 0.0038
Endosome 0.2002 0.0855 0.11 0.1264 0.0584 0.1316 0.1788 0.1354 0.1473 0.2011 0.1454 0.1166 0.1606 0.1015 0.1653 0.2013 0.1629 0.1386
Golgi 0.0075 0.0016 0.0025 0.0014 0.0033 0.0025 0.0105 0.0048 0.0064 0.0103 0.003 0.0045 0.0074 0.0026 0.0054 0.006 0.004 0.0036
Lipid Particles 0.0256 0.0056 0.0122 0.0064 0.0053 0.0128 0.0199 0.0084 0.0341 0.0087 0.0073 0.0217 0.0217 0.0091 0.0119 0.0201 0.0068 0.0202
Mitochondria 0.0238 0.0092 0.0304 0.0015 0.0026 0.0151 0.0445 0.0241 0.0625 0.053 0.0856 0.0329 0.0383 0.0276 0.0479 0.017 0.0057 0.0113
None 0.0881 0.1378 0.1271 0.07 0.0018 0.03 0.0946 0.091 0.1162 0.0225 0.0757 0.0295 0.1216 0.0806 0.1241 0.1179 0.1742 0.0616
Nuclear Periphery 0.012 0.0055 0.0108 0.0038 0.0288 0.0132 0.0061 0.0047 0.0053 0.0023 0.002 0.0052 0.0069 0.014 0.01 0.0047 0.0187 0.0077
Nucleolus 0.0043 0.0092 0.0146 0.0092 0.0033 0.0037 0.0086 0.0024 0.0157 0.0096 0.0017 0.005 0.0067 0.0126 0.0082 0.0049 0.0015 0.0076
Nucleus 0.0141 0.0134 0.0161 0.0054 0.0125 0.019 0.0064 0.0024 0.012 0.0221 0.0016 0.0054 0.0092 0.0101 0.0099 0.0038 0.003 0.017
Peroxisomes 0.0021 0.0011 0.0027 0.0009 0.0006 0.0009 0.0086 0.001 0.0048 0.0098 0.0007 0.0066 0.0087 0.0016 0.0032 0.0037 0.0004 0.0074
Punctate Nuclear 0.0034 0.0013 0.0022 0.0009 0.0062 0.0008 0.0049 0.0002 0.0034 0.0017 0.0001 0.0011 0.0022 0.0018 0.0034 0.0027 0.0004 0.0009
Vacuole 0.395 0.5216 0.4573 0.6094 0.6473 0.5403 0.3602 0.4207 0.336 0.4636 0.4578 0.4999 0.38 0.5042 0.3565 0.4059 0.4266 0.49
Vacuole Periphery 0.1478 0.1337 0.1474 0.1339 0.1852 0.177 0.1701 0.2436 0.1739 0.1484 0.2018 0.2265 0.1407 0.1288 0.1652 0.14 0.11 0.1544

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.0344 4.2076 5.7984 8.2156 1.3077 7.3923 5.4698 6.358 8.9929 4.5757
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Low affinity methionine permease; similar to Mup1p
Localization
Cell Percentages vacuole (30%), cytoplasm (5%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mup3

Mup3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mup3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available