Standard name
Human Ortholog
Description ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.86 0.71 0.8 0.8 0.87 0.87 0.88 – 0.77 0.84 0.77 0.33 0.33 0.37 0.47 0.43 0.36 0.57 0.34 0.13 0.56 0.57 0.53
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0.05 0.05 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0.27 0.31 0.21 0 0 0.05 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.19 0.19 0.17 – 0.31 0.28 0.35 0 0 0 0.06 0 0 0.06 0 0 0.07 0.12 0.08
Nucleus 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0.24 0.28 0.25 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.07 0.31 0.25 0.24 0.1 0.1 0.07 – 0.05 0.06 0.06 0.44 0.42 0.48 0.39 0.39 0.49 0.24 0.48 0.71 0.22 0.18 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 1 0 0 0 – 0 0 0 17 14 18 0 1 0 2 1 3 1 4 9
Bud 0 3 0 2 5 4 6 8 – 5 7 6 2 2 3 0 0 0 7 5 8 12 6 13
Bud Neck 4 0 2 0 0 1 2 3 – 1 4 0 0 1 1 0 0 0 2 3 2 3 3 2
Bud Site 1 0 0 0 0 0 0 0 – 2 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 0 0 0 0 2 – 1 1 2 3 3 4 0 1 0 2 0 0 1 1 2
Cytoplasm 391 245 314 252 317 362 407 497 – 274 301 228 264 264 317 222 301 110 251 112 62 173 178 199
Endoplasmic Reticulum 2 11 1 0 0 1 0 0 – 1 0 0 36 41 43 14 27 17 3 2 5 2 4 3
Endosome 2 1 17 0 3 2 1 0 – 0 0 0 215 246 183 10 21 15 14 16 17 5 7 5
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 10 12 6 0 2 1 2 0 1 1 3 4
Mitochondria 8 14 8 6 15 77 87 95 – 112 99 105 0 0 0 30 30 2 25 7 14 21 37 28
Nucleus 1 2 17 20 9 8 3 9 – 5 8 2 4 4 19 111 199 76 1 1 0 1 0 1
Nuclear Periphery 0 2 0 1 1 4 7 1 – 3 1 3 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 1 0 0 1 0 1 0 – 2 0 0 2 2 11 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 – 2 0 0 1 1 0 0 0 0 1 0 1 0 1 0
SpindlePole 1 0 2 0 2 2 2 1 – 9 1 3 33 32 28 1 0 0 10 7 6 5 4 4
Vac/Vac Membrane 26 21 135 79 93 40 45 42 – 19 23 17 350 339 411 181 274 149 106 158 328 69 56 91
Unique Cell Count 420 286 440 315 394 416 466 563 356 360 298 791 802 855 469 701 303 443 328 463 309 314 375
Labelled Cell Count 437 302 497 360 448 501 561 658 436 445 368 937 961 1044 569 857 371 443 328 463 309 314 375


Endosome, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.0 4.6 4.6 3.8 3.2 3.1 3.3 – 2.9 3.0 3.0 9.3 9.3 9.8 5.4 6.1 6.9 5.5 5.4 5.8
Std Deviation (1e-4) 0.8 0.8 1.4 2.1 1.4 1.2 1.2 1.0 – 0.9 1.2 1.1 2.4 2.4 2.6 1.7 2.0 2.3 1.6 1.9 2.2
Intensity Change (Log2) – – – 0.01 -0.26 -0.53 -0.57 -0.49 – -0.68 -0.61 -0.62 1.02 1.02 1.09 0.23 0.42 0.6 0.25 0.24 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.5 2.9 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4162 0.9356 0.4964 0.4887 0.9376 0.8734 0.172 0.7091 0.3261 0.5942 -0.1228 0.3763 1.7407 5.0179 3.6069 4.2815 2.538 3.8801
Actin 0.0056 0.0001 0.0001 0.0005 0.0004 0.0002 0.0348 0.0003 0.0143 0.0062 0.0007 0.0038 0.0028 0.0004 0.0002 0 0.006 0
Bud 0.0034 0.0052 0.0047 0.0008 0.0146 0.0084 0.0096 0.0144 0.0035 0.0006 0.0359 0.0046 0.0004 0.0002 0.0007 0.0003 0.0009 0.0006
Bud Neck 0.001 0.0049 0.0023 0.0025 0.0023 0.003 0.0081 0.0018 0.002 0.0012 0.001 0.0024 0.0011 0.001 0.001 0.0027 0.0015 0.0017
Bud Periphery 0.0023 0.0041 0.0029 0.0025 0.0292 0.0038 0.0033 0.0082 0.0063 0.0012 0.0112 0.0029 0.001 0.0007 0.0013 0.001 0.0022 0.0026
Bud Site 0.005 0.0253 0.0092 0.0026 0.004 0.0022 0.0071 0.0218 0.0111 0.0034 0.002 0.0009 0.0023 0.0007 0.0016 0.0014 0.002 0.0011
Cell Periphery 0.0013 0.0038 0.0022 0.0036 0.0023 0.0028 0.0009 0.0017 0.0011 0.0005 0.0008 0.0007 0.0035 0.0048 0.0055 0.0057 0.0043 0.0077
Cytoplasm 0.0424 0.0541 0.0397 0.0076 0.0111 0.0475 0.0603 0.1282 0.1125 0.0461 0.1064 0.1424 0.0058 0.0108 0.0088 0.0012 0.0044 0.0021
Cytoplasmic Foci 0.0448 0.0334 0.0413 0.0137 0.0099 0.0625 0.075 0.0521 0.0604 0.0397 0.0362 0.0553 0.0213 0.0022 0.0085 0.003 0.0278 0.0019
Eisosomes 0.0002 0 0.0001 0.0003 0.0001 0 0.0003 0 0.0002 0 0 0.0003 0.0011 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0071 0.0065 0.0045 0.0174 0.0016 0.0032 0.0047 0.0041 0.0058 0.0028 0.0038 0.0045 0.0062 0.0113 0.0074 0.0017 0.0023 0.0042
Endosome 0.2455 0.173 0.2202 0.2734 0.1941 0.1912 0.1798 0.1567 0.1536 0.2965 0.178 0.1803 0.2493 0.0848 0.1885 0.1299 0.1716 0.1025
Golgi 0.0068 0.0033 0.0045 0.0052 0.003 0.0028 0.0088 0.003 0.0074 0.0082 0.0082 0.0038 0.0116 0.0018 0.0111 0.0061 0.009 0.0039
Lipid Particles 0.0243 0.0134 0.0203 0.0235 0.0254 0.0119 0.0273 0.0077 0.0194 0.0209 0.0166 0.0061 0.0397 0.0103 0.0249 0.0168 0.0619 0.0182
Mitochondria 0.0095 0.0156 0.0166 0.0067 0.0018 0.0111 0.0208 0.0052 0.0247 0.0037 0.0204 0.0091 0.0138 0.0067 0.027 0.0066 0.0093 0.0021
None 0.1346 0.065 0.0754 0.0536 0.0092 0.0478 0.3195 0.253 0.3288 0.1372 0.2784 0.2373 0.0227 0.0107 0.0117 0.0002 0.0036 0.0001
Nuclear Periphery 0.0128 0.0132 0.0136 0.014 0.004 0.007 0.0077 0.0037 0.006 0.008 0.0042 0.0063 0.015 0.0187 0.0053 0.0023 0.0172 0.015
Nucleolus 0.0132 0.011 0.0098 0.0057 0.015 0.0062 0.003 0.0057 0.0057 0.0065 0.0015 0.0027 0.0136 0.003 0.0026 0.0098 0.0047 0.0048
Nucleus 0.0281 0.02 0.0166 0.0109 0.0152 0.0222 0.0059 0.0109 0.0106 0.0076 0.0049 0.0106 0.0065 0.0046 0.0024 0.0036 0.0108 0.0046
Peroxisomes 0.0047 0.0016 0.0032 0.0016 0.0007 0.0029 0.0157 0.0038 0.0058 0.0215 0.0031 0.0053 0.0022 0.0002 0.0013 0.0006 0.038 0.0003
Punctate Nuclear 0.004 0.0031 0.0085 0.0006 0.0303 0.002 0.0106 0.0029 0.0084 0.0024 0.0013 0.005 0.0051 0.0001 0.0002 0.0001 0.0088 0.0001
Vacuole 0.3424 0.4805 0.4177 0.5063 0.5727 0.499 0.1792 0.2819 0.1873 0.3346 0.256 0.2844 0.4319 0.6648 0.5392 0.6841 0.528 0.6728
Vacuole Periphery 0.0611 0.0629 0.0866 0.047 0.0531 0.0623 0.0176 0.0329 0.0252 0.0512 0.0293 0.0313 0.1431 0.1619 0.1508 0.1228 0.0856 0.1536

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.377 37.1724 110.334 100.2624 19.7431 45.0717 97.2708 119.8694 122.9969 108.7934
Translational Efficiency 0.5981 0.7812 0.7341 0.639 0.6792 0.6618 0.7516 0.6824 0.7818 0.6218

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
127 956 1875 913 2065 2062 1875 1113 2192 3018 3750 2026

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 934.04 985.86 759.79 890.23 1384.48 745.83 781.76 932.73 1358.38 821.86 770.77 913.58
Standard Deviation 217.33 335.51 132.26 152.01 329.70 103.20 129.23 176.23 340.90 235.38 131.22 167.10
Intensity Change Log 2 — 0.077898 -0.297884 -0.069306 — -0.892425 -0.824547 -0.569813 — -0.421023 -0.588822 -0.346921

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003439 0.000153 0.000700 0.000974 0.000024 0.000325 0.001482 0.000665 0.000221 0.000271 0.001091 0.000804
Bud Neck 0.010092 0.016485 0.024407 0.016199 0.001376 0.048132 0.031063 0.012439 0.001881 0.038107 0.027735 0.014133
Bud Site 0.031906 0.044357 0.017512 0.044973 0.002639 0.023302 0.049731 0.049192 0.004335 0.029972 0.033622 0.047291
Cell Periphery 0.000476 0.005785 0.000178 0.000327 0.018043 0.000432 0.000303 0.000295 0.017025 0.002128 0.000241 0.000309
Cytoplasm 0.099126 0.120865* 0.388300* 0.195182* 0.000677 0.232458* 0.330746* 0.185584* 0.006381 0.197110* 0.359523* 0.189909*
Cytoplasmic Foci 0.070340 0.033478 0.039686 0.061814 0.000560 0.140929 0.074421 0.058503 0.004603 0.106892 0.057054 0.059995
Eisosomes 0.000091 0.000056 0.000074 0.000040 0.000135 0.000186 0.000056 0.000031 0.000132 0.000145 0.000065 0.000035
Endoplasmic Reticulum 0.003751 0.003944 0.004148 0.004159 0.005636 0.002705 0.004181 0.002916 0.005526 0.003097 0.004164 0.003476
Endosome 0.068141 0.044085 0.036699 0.080998 0.007196 0.080765 0.085419 0.115457 0.010727 0.069146 0.061059 0.099928
Golgi 0.017530 0.004039 0.001096 0.008034 0.001968 0.006027 0.003995 0.010313 0.002870 0.005398 0.002546 0.009286
Lipid Particles 0.005253 0.001642 0.003895 0.003537 0.000653 0.011223 0.009265 0.003427 0.000920 0.008188 0.006580 0.003476
Mitochondria 0.002844 0.003454 0.001071 0.003130 0.001049 0.015265 0.002540 0.002078 0.001153 0.011524 0.001805 0.002552
Mitotic Spindle 0.004927 0.000923 0.008489 0.007427 0.000087 0.003850 0.012641 0.006362 0.000367 0.002923 0.010565 0.006842
None 0.000233 0.003673 0.003808 0.000756 0.000068 0.001255 0.002260 0.000469 0.000078 0.002021 0.003034 0.000598
Nuclear Periphery 0.001219 0.000167 0.002652 0.000955 0.000459 0.000558 0.001301 0.000624 0.000503 0.000434 0.001976 0.000773
Nuclear Periphery Foci 0.001990 0.000455 0.004381 0.002814 0.000523 0.000278 0.002372 0.001356 0.000608 0.000334 0.003377 0.002013
Nucleolus 0.006534 0.002476 0.003029 0.001139 0.000825 0.001255 0.001380 0.000718 0.001156 0.001642 0.002204 0.000908
Nucleus 0.050653 0.017903 0.300243* 0.029817 0.000442 0.044667 0.084238 0.021540 0.003351 0.036189 0.192241* 0.025270
Peroxisomes 0.002984 0.002020 0.003935 0.003339 0.000053 0.010177 0.006993 0.003417 0.000223 0.007593 0.005464 0.003382
Vacuole 0.586907* 0.617908* 0.154051 0.518600* 0.838359* 0.367152* 0.291516* 0.496312* 0.823791* 0.446583* 0.222784* 0.506356*
Vacuole Periphery 0.031563 0.076131 0.001643 0.015786 0.119228* 0.009056 0.004098 0.028303 0.114149* 0.030303 0.002871 0.022663

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.23 1.00 0.92 -1.87 -0.40 -5.25 -4.94 -3.50 -1.61 2.39 -0.53 -3.99 -2.23 -2.29 1.28
Bud Neck -3.06 -6.75 -2.98 0.61 4.54 -21.48 -17.37 -12.63 15.63 9.81 -22.38 -21.10 -15.51 14.00 10.00
Bud Site -1.38 1.33 -0.85 1.18 -6.01 -12.33 -14.96 -12.67 -5.77 -0.02 -15.54 -14.95 -15.21 -4.25 -4.07
Cell Periphery -8.32 1.32 0.72 8.93 -2.09 14.23 14.37 14.38 2.99 0.54 12.58 14.38 14.34 9.10 -1.35
Cytoplasm -0.26 -13.68 -5.36 -8.40 13.70 -41.21* -47.35 -26.62 2.21 11.23 -39.67* -67.76* -35.32 -2.24 17.69
Cytoplasmic Foci 3.44 2.92 1.33 -4.37 -3.89 -36.21* -26.38 -20.40 18.46 4.44 -33.44 -28.96 -24.00 14.46 0.49
Eisosomes 2.31 0.46 3.46 2.81 1.14 -1.80 3.25 4.29 9.63 7.46 -0.52 2.55 4.25 9.77 2.07
Endoplasmic Reticulum -0.60 -1.31 -0.23 0.52 1.58 7.05 2.72 6.03 0.05 2.05 6.50 3.02 5.95 -0.00 2.69
Endosome 2.37 3.97 -0.46 -4.20 -7.04 -24.93 -18.98 -18.37 -2.74 -4.06 -24.95 -19.59 -21.88 -3.37 -7.74
Golgi 2.03 2.61 1.62 -1.61 -4.34 -5.46 -1.85 -4.96 -1.70 -3.88 -3.84 1.19 -4.62 -2.03 -5.88
Lipid Particles 2.65 0.56 1.29 -4.15 2.12 -12.99 -10.85 -7.12 8.63 6.33 -12.76 -13.59 -9.19 7.75 6.53
Mitochondria -0.63 1.52 0.01 0.58 -1.25 -10.85 -3.25 -1.00 9.16 0.66 -10.53 -2.03 -1.45 8.17 -0.63
Mitotic Spindle 1.74 -1.75 -1.54 -4.06 -0.13 -5.31 -8.50 -5.50 -2.48 2.74 -5.12 -11.03 -6.75 -4.23 1.83
None -4.87 -11.48 -2.82 4.05 8.91 -6.56 -5.37 -4.80 3.75 4.10 -7.77 -11.93 -6.57 5.20 8.88
Nuclear Periphery 3.05 -4.17 0.25 -6.70 5.71 -0.63 -5.77 -2.00 -2.10 4.86 0.42 -8.21 -2.64 -5.24 7.38
Nuclear Periphery Foci 2.20 -3.84 -1.19 -4.25 2.89 1.73 -6.81 -4.28 -6.53 3.38 1.88 -8.86 -5.01 -6.44 4.16
Nucleolus 1.02 0.54 1.46 2.40 4.86 -2.47 -3.30 0.24 2.31 3.09 -1.89 -4.61 0.75 3.08 5.73
Nucleus 3.01 -19.18 1.07 -6.10 35.74 -22.78 -26.10 -13.22 4.17 14.84 -19.67 -46.03 -16.24 0.23 35.81
Peroxisomes 1.16 -1.62 -0.13 -1.38 1.57 -12.68 -11.04 -7.63 8.09 4.73 -13.23 -12.76 -9.76 7.55 4.50
Vacuole -0.96 14.56 2.23 6.46 -30.94 56.30* 69.90* 34.65* -11.22 -17.38 48.56* 94.87* 39.54 -6.60 -33.97
Vacuole Periphery -5.51 3.51 2.02 13.95 -8.20 28.16* 29.68* 22.34 -7.19 -10.21 20.93 30.00 24.56 6.13 -12.98
Standard name
Human Ortholog
Description ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress
Localization
Cell Percentages vacuole (79%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Arn1

Arn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available