Standard name
Human Ortholog
Description Subunit of the ubiqunol-cytochrome c oxidoreductase complex; this complex comprises part of the mitochondrial respiratory chain; members include Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0.05 0.08
Bud 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0.05 0.09 0.05 0.09 0.06 0.08 0.05
Golgi 0 0.06 0 0 0 0 0 0 0 0 0 0.18 0.19 0.21 0.41 0.09 0.1 0 0.13 0.18 0.11
Mitochondria 0.85 0.89 0.95 0.95 0.92 0.9 0.88 0.94 0.91 0.94 0.96 0.82 0.84 0.75 0 0.62 0.66 0.63 0.6 0.52 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.06 0.11 0.08 0.13 0.08 0.1 0.1 0 0 0 0.09 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.07 0.1 0.14 0.1 0.12 0.09 0.09 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0.08 0.07 0.05 0.18 0.06 0.08 0.18 0.12 0.09 0.11
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.1 0.05 0 0.09 0 0 0 0 0 0 0.13 0.08 0.12 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 2 5 2 1 0 1 0 1 0 1 1 1 4 1 23 19 11 6 9 22
Bud 15 5 34 17 9 13 16 8 14 9 13 0 1 3 1 2 0 1 0 1 3
Bud Neck 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0
Bud Site 2 0 16 2 1 3 2 6 6 8 10 0 0 0 0
Cell Periphery 20 17 9 15 4 1 6 1 1 6 4 7 9 8 1 0 0 0 0 0 0
Cytoplasm 6 3 6 14 15 14 15 5 5 5 2 5 3 9 3 0 0 0 0 0 2
Endoplasmic Reticulum 5 0 3 10 1 4 1 1 3 3 3 4 3 8 1 1 0 0 0 0 1
Endosome 52 13 2 1 7 6 7 2 2 0 1 14 19 23 1 62 18 44 9 16 14
Golgi 5 21 1 0 0 2 1 0 0 0 4 45 68 70 9 57 36 21 22 38 31
Mitochondria 551 295 470 429 332 503 593 563 550 362 538 209 310 246 0 418 234 312 103 110 157
Nucleus 23 1 4 2 1 7 12 5 3 0 0 2 0 0 0 18 1 0 0 0 0
Nuclear Periphery 15 12 11 15 23 63 54 77 48 38 55 8 6 10 2 6 0 2 2 1 7
Nucleolus 10 10 20 11 26 56 96 62 72 33 51 6 6 5 1 4 0 0 0 2 0
Peroxisomes 35 16 27 7 3 6 8 2 5 2 4 20 24 16 4 40 29 88 20 19 31
SpindlePole 12 0 24 1 3 3 5 5 4 1 3 2 5 3 1 6 1 4 1 0 2
Vac/Vac Membrane 83 33 23 20 32 22 29 22 10 7 12 33 30 38 0 22 10 3 3 8 6
Unique Cell Count 647 333 493 452 362 556 677 599 604 385 562 255 367 329 22 671 355 499 173 212 282
Labelled Cell Count 841 428 655 546 459 703 846 759 724 474 701 356 485 443 25 671 355 499 173 212 282


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 8.8 4.9 6.7 6.1 6.8 8.1 8.2 8.6 9.1 9.7 8.5 8.6 8.9 8.5 8.8 9.5 9.7
Std Deviation (1e-4) 1.0 1.8 1.3 2.0 1.7 2.1 2.6 3.1 3.0 3.6 3.8 1.5 1.6 1.9 2.1 3.2 2.0 2.4
Intensity Change (Log2) 0.44 0.31 0.46 0.73 0.74 0.81 0.9 0.98 0.79 0.82 0.86 0.79 0.85 0.95 0.99


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud -2.1 -2.9 -3.6 -3.8 -4.8 -3.7 -3.1 -3.6 -4.3 -4.9 -4.1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -3.1 -3.1 -3.3 -4.0 -2.6 -2.6 -1.1 -1.5 -2.9 -3.5 -3.3 0
Cell Periphery 1.5 -0.9 0 -1.4 0 0 -0.3 -1.6 0.8 0.6 0.6 0
Cytoplasm 2.0 2.7 1.5 1.3 0 0 0 0 0 0 1.6 0
Endoplasmic Reticulum 2.1 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 4.6 4.5 5.4 0
Golgi 0 0 0 0 0 0 0 0 9.4 9.8 10.5 0
Mitochondria -0.3 -2.2 -3.0 -4.5 -1.0 -2.8 -0.9 0.3 -6.0 -5.4 -8.6 -15.5
Nucleus 0 0 0.7 1.4 0 0 0 0 0 0 0 0
Nuclear Periphery 1.0 3.0 5.7 4.2 6.4 4.2 4.9 5.1 0.7 -0.6 0.7 0
Nucleolus -1.4 2.0 3.8 5.7 3.9 4.7 2.8 3.2 -1.2 -2.1 -2.1 0
Peroxisomes -3.2 -3.6 -4.1 -4.3 -5.3 -4.6 -4.1 -4.6 1.3 0.7 -0.4 0
SpindlePole -4.4 -3.3 -4.4 -4.5 -4.1 -4.4 -4.1 -4.4 -2.9 -2.8 -3.1 0
Vacuole -0.2 2.5 -0.6 -0.3 -0.8 -2.9 -2.3 -2.3 4.1 2.1 3.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5039 16.8611 18.7675 33.0437 24.4549 23.564 24.5069 30.2044 20.2711 26.3229
Translational Efficiency 4.0041 2.7547 2.981 1.8282 2.6021 4.6763 2.7955 2.0277 3.1968 1.9926

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
25 2172 1619 605 1362 1311 12 538 1387 3483 1631 1143

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1462.99 882.98 1044.58 1295.84 699.60 975.00 562.38 1267.60 713.36 917.62 1041.03 1282.55
Standard Deviation 352.01 156.57 223.68 256.72 117.15 267.23 129.46 247.58 161.32 210.13 226.91 252.86
Intensity Change Log 2 -0.728467 -0.485997 -0.175032 0.478872 -0.314985 0.857497 -0.219025 -0.428426 0.245321

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003969 0.009150 0.004145 0.024151 0.009985 0.006248 0.003327 0.036824 0.009876 0.008058 0.004139 0.030116
Bud Neck 0.000910 0.001036 0.005395 0.002534 0.002282 0.002219 0.029568 0.002149 0.002257 0.001481 0.005573 0.002353
Bud Site 0.000232 0.003859 0.015005 0.017534 0.004885 0.007321 0.107467* 0.020578 0.004801 0.005162 0.015685 0.018967
Cell Periphery 0.000235 0.000190 0.000609 0.000867 0.000538 0.000394 0.053435 0.001196 0.000532 0.000267 0.000997 0.001022
Cytoplasm 0.000184 0.001187 0.003756 0.002118 0.001214 0.001624 0.175183* 0.002465 0.001195 0.001352 0.005018 0.002281
Cytoplasmic Foci 0.065061 0.041798 0.034674 0.020537 0.044759 0.030756 0.030874 0.023610 0.045125 0.037642 0.034646 0.021984
Eisosomes 0.000974 0.000124 0.000103 0.000280 0.000164 0.000133 0.000715 0.000405 0.000179 0.000127 0.000107 0.000339
Endoplasmic Reticulum 0.000514 0.000274 0.001545 0.001926 0.000429 0.000420 0.010292 0.001784 0.000430 0.000329 0.001609 0.001859
Endosome 0.010612 0.056988 0.063373 0.027107 0.096261 0.055771 0.001499 0.027546 0.094717 0.056530 0.062918 0.027314
Golgi 0.058553 0.075641 0.075648 0.148233* 0.077256 0.059288 0.000358 0.120898* 0.076919 0.069486 0.075094 0.135367*
Lipid Particles 0.022201 0.007501 0.007747 0.002478 0.021571 0.008930 0.000510 0.001219 0.021582 0.008039 0.007694 0.001885
Mitochondria 0.812447* 0.747567* 0.659471* 0.674729* 0.655899* 0.775429* 0.002400 0.704098* 0.658721* 0.758054* 0.654637* 0.688553*
Mitotic Spindle 0.000563 0.005492 0.023309 0.016275 0.002513 0.004770 0.000203 0.014987 0.002478 0.005220 0.023139 0.015669
None 0.000882 0.000178 0.000748 0.000875 0.000224 0.000470 0.148580* 0.000558 0.000236 0.000288 0.001836 0.000726
Nuclear Periphery 0.000314 0.000092 0.002436 0.000375 0.000050 0.000395 0.000388 0.000180 0.000054 0.000206 0.002421 0.000283
Nuclear Periphery Foci 0.000317 0.000131 0.002083 0.000434 0.000279 0.000571 0.019703 0.000178 0.000280 0.000297 0.002213 0.000313
Nucleolus 0.000063 0.000140 0.000742 0.000093 0.000907 0.000605 0.005568 0.000070 0.000892 0.000315 0.000778 0.000082
Nucleus 0.000041 0.000162 0.001478 0.000097 0.000385 0.000223 0.396926* 0.000070 0.000379 0.000185 0.004388 0.000084
Peroxisomes 0.017261 0.043073 0.012112 0.012847 0.076102 0.025969 0.000186 0.021606 0.075041 0.036635 0.012024 0.016970
Vacuole 0.001315 0.002238 0.041592 0.017709 0.001615 0.008820 0.012513 0.009055 0.001610 0.004715 0.041378 0.013636
Vacuole Periphery 0.003351 0.003177 0.044029 0.028802 0.002683 0.009644 0.000305 0.010522 0.002695 0.005611 0.043707 0.020197

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.44 -0.16 -4.88 -3.62 -4.97 NAN NAN NAN NAN NAN 1.39 4.96 -6.16 -6.89 -8.26
Bud Neck -0.36 -5.59 -2.85 -2.79 3.17 NAN NAN NAN NAN NAN 1.71 -3.87 -0.21 -2.49 3.95
Bud Site -5.60 -10.52 -5.44 -4.22 -0.70 NAN NAN NAN NAN NAN -0.33 -6.28 -5.73 -5.89 -1.18
Cell Periphery 0.60 -2.45 -3.78 -4.50 -1.28 NAN NAN NAN NAN NAN 4.87 -1.64 -4.02 -6.79 -0.13
Cytoplasm -5.79 -5.48 -4.17 -1.98 2.17 NAN NAN NAN NAN NAN -0.57 -4.34 -2.93 -2.85 3.09
Cytoplasmic Foci 0.78 1.01 1.49 7.65 4.96 NAN NAN NAN NAN NAN 2.92 3.50 8.47 7.41 4.95
Eisosomes 1.39 1.42 1.13 -5.24 -5.90 NAN NAN NAN NAN NAN 3.56 4.56 -4.83 -6.89 -7.40
Endoplasmic Reticulum 1.21 -3.87 -3.09 -3.81 -0.79 NAN NAN NAN NAN NAN 1.31 -5.51 -3.84 -4.13 -0.58
Endosome -8.22 -8.96 -2.93 9.28 9.95 NAN NAN NAN NAN NAN 8.48 6.36 15.25 11.97 10.93
Golgi -0.65 -0.64 -3.22 -7.19 -7.01 NAN NAN NAN NAN NAN 1.85 0.39 -7.93 -9.55 -8.01
Lipid Particles 0.99 0.97 1.33 4.06 4.20 NAN NAN NAN NAN NAN 5.52 5.46 8.31 6.83 5.26
Mitochondria 0.97 2.30 2.02 4.49 -0.94 NAN NAN NAN NAN NAN -9.03 0.38 -2.11 5.95 -2.56
Mitotic Spindle -7.48 -11.39 -5.57 -3.76 2.11 NAN NAN NAN NAN NAN -3.17 -9.93 -6.61 -5.37 2.85
None 2.44 0.41 0.03 -10.53 -1.26 NAN NAN NAN NAN NAN -0.58 -2.77 -7.97 -9.61 1.95
Nuclear Periphery 1.29 -4.33 -0.34 -4.28 4.45 NAN NAN NAN NAN NAN -2.66 -5.19 -6.33 -1.15 4.67
Nuclear Periphery Foci 0.99 -4.44 -0.53 -2.27 4.37 NAN NAN NAN NAN NAN 0.24 -5.09 0.10 -0.16 5.17
Nucleolus -0.90 -4.78 -0.63 0.59 4.69 NAN NAN NAN NAN NAN 1.61 0.35 2.28 1.94 5.05
Nucleus -0.99 -2.85 -1.34 0.51 2.73 NAN NAN NAN NAN NAN 1.04 -3.24 1.65 1.23 3.54
Peroxisomes -1.61 0.32 0.27 8.06 -0.24 NAN NAN NAN NAN NAN 8.24 14.26 12.24 6.87 -2.01
Vacuole -1.63 -18.47 -6.19 -5.96 7.12 NAN NAN NAN NAN NAN -7.53 -18.91 -7.70 -5.57 10.59
Vacuole Periphery 0.14 -16.03 -7.22 -7.73 3.81 NAN NAN NAN NAN NAN -6.93 -18.58 -9.18 -7.52 8.07
Standard name
Human Ortholog
Description Subunit of the ubiqunol-cytochrome c oxidoreductase complex; this complex comprises part of the mitochondrial respiratory chain; members include Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Qcr10

Qcr10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Qcr10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available