Standard name
Human Ortholog
Description Subunit of the ubiqunol-cytochrome c oxidoreductase complex; this complex comprises part of the mitochondrial respiratory chain; members include Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0.05 0.08
Bud 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0.05 0.09 0.05 0.09 0.06 0.08 0.05
Golgi 0 0.06 0 0 0 0 0 0 0 0 0 0.18 0.19 0.21 0.41 0.09 0.1 0 0.13 0.18 0.11
Mitochondria 0.85 0.89 0.95 0.95 0.92 0.9 0.88 0.94 0.91 0.94 0.96 0.82 0.84 0.75 0 0.62 0.66 0.63 0.6 0.52 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.06 0.11 0.08 0.13 0.08 0.1 0.1 0 0 0 0.09 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.07 0.1 0.14 0.1 0.12 0.09 0.09 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0.08 0.07 0.05 0.18 0.06 0.08 0.18 0.12 0.09 0.11
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.1 0.05 0 0.09 0 0 0 0 0 0 0.13 0.08 0.12 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 2 5 2 1 0 1 0 1 0 1 1 1 4 1 23 19 11 6 9 22
Bud 15 5 34 17 9 13 16 8 14 9 13 0 1 3 1 2 0 1 0 1 3
Bud Neck 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0
Bud Site 2 0 16 2 1 3 2 6 6 8 10 0 0 0 0
Cell Periphery 20 17 9 15 4 1 6 1 1 6 4 7 9 8 1 0 0 0 0 0 0
Cytoplasm 6 3 6 14 15 14 15 5 5 5 2 5 3 9 3 0 0 0 0 0 2
Endoplasmic Reticulum 5 0 3 10 1 4 1 1 3 3 3 4 3 8 1 1 0 0 0 0 1
Endosome 52 13 2 1 7 6 7 2 2 0 1 14 19 23 1 62 18 44 9 16 14
Golgi 5 21 1 0 0 2 1 0 0 0 4 45 68 70 9 57 36 21 22 38 31
Mitochondria 551 295 470 429 332 503 593 563 550 362 538 209 310 246 0 418 234 312 103 110 157
Nucleus 23 1 4 2 1 7 12 5 3 0 0 2 0 0 0 18 1 0 0 0 0
Nuclear Periphery 15 12 11 15 23 63 54 77 48 38 55 8 6 10 2 6 0 2 2 1 7
Nucleolus 10 10 20 11 26 56 96 62 72 33 51 6 6 5 1 4 0 0 0 2 0
Peroxisomes 35 16 27 7 3 6 8 2 5 2 4 20 24 16 4 40 29 88 20 19 31
SpindlePole 12 0 24 1 3 3 5 5 4 1 3 2 5 3 1 6 1 4 1 0 2
Vac/Vac Membrane 83 33 23 20 32 22 29 22 10 7 12 33 30 38 0 22 10 3 3 8 6
Unique Cell Count 647 333 493 452 362 556 677 599 604 385 562 255 367 329 22 671 355 499 173 212 282
Labelled Cell Count 841 428 655 546 459 703 846 759 724 474 701 356 485 443 25 671 355 499 173 212 282


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 8.8 4.9 6.7 6.1 6.8 8.1 8.2 8.6 9.1 9.7 8.5 8.6 8.9 8.5 8.8 9.5 9.7
Std Deviation (1e-4) 1.0 1.8 1.3 2.0 1.7 2.1 2.6 3.1 3.0 3.6 3.8 1.5 1.6 1.9 2.1 3.2 2.0 2.4
Intensity Change (Log2) 0.44 0.31 0.46 0.73 0.74 0.81 0.9 0.98 0.79 0.82 0.86 0.79 0.85 0.95 0.99

WT3RAP60RAP140RAP220RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_20510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud -2.1 -2.9 -3.6 -3.8 -4.8 -3.7 -3.1 -3.6 -4.3 -4.9 -4.1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -3.1 -3.1 -3.3 -4.0 -2.6 -2.6 -1.1 -1.5 -2.9 -3.5 -3.3 0
Cell Periphery 1.5 -0.9 0 -1.4 0 0 -0.3 -1.6 0.8 0.6 0.6 0
Cytoplasm 2.0 2.7 1.5 1.3 0 0 0 0 0 0 1.6 0
Endoplasmic Reticulum 2.1 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 4.6 4.5 5.4 0
Golgi 0 0 0 0 0 0 0 0 9.4 9.8 10.5 0
Mitochondria -0.3 -2.2 -3.0 -4.5 -1.0 -2.8 -0.9 0.3 -6.0 -5.4 -8.6 -15.5
Nucleus 0 0 0.7 1.4 0 0 0 0 0 0 0 0
Nuclear Periphery 1.0 3.0 5.7 4.2 6.4 4.2 4.9 5.1 0.7 -0.6 0.7 0
Nucleolus -1.4 2.0 3.8 5.7 3.9 4.7 2.8 3.2 -1.2 -2.1 -2.1 0
Peroxisomes -3.2 -3.6 -4.1 -4.3 -5.3 -4.6 -4.1 -4.6 1.3 0.7 -0.4 0
SpindlePole -4.4 -3.3 -4.4 -4.5 -4.1 -4.4 -4.1 -4.4 -2.9 -2.8 -3.1 0
Vacuole -0.2 2.5 -0.6 -0.3 -0.8 -2.9 -2.3 -2.3 4.1 2.1 3.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5039 16.8611 18.7675 33.0437 24.4549 23.564 24.5069 30.2044 20.2711 26.3229
Translational Efficiency 4.0041 2.7547 2.981 1.8282 2.6021 4.6763 2.7955 2.0277 3.1968 1.9926

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
25 2172 1619 605 1362 1311 12 538 1387 3483 1631 1143

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1462.99 882.98 1044.58 1295.84 699.60 975.00 562.38 1267.60 713.36 917.62 1041.03 1282.55
Standard Deviation 352.01 156.57 223.68 256.72 117.15 267.23 129.46 247.58 161.32 210.13 226.91 252.86
Intensity Change Log 2 -0.728467 -0.485997 -0.175032 0.478872 -0.314985 0.857497 -0.219025 -0.428426 0.245321

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003969 0.009150 0.004145 0.024151 0.009985 0.006248 0.003327 0.036824 0.009876 0.008058 0.004139 0.030116
Bud Neck 0.000910 0.001036 0.005395 0.002534 0.002282 0.002219 0.029568 0.002149 0.002257 0.001481 0.005573 0.002353
Bud Site 0.000232 0.003859 0.015005 0.017534 0.004885 0.007321 0.107467 0.020578 0.004801 0.005162 0.015685 0.018967
Cell Periphery 0.000235 0.000190 0.000609 0.000867 0.000538 0.000394 0.053435 0.001196 0.000532 0.000267 0.000997 0.001022
Cytoplasm 0.000184 0.001187 0.003756 0.002118 0.001214 0.001624 0.175183 0.002465 0.001195 0.001352 0.005018 0.002281
Cytoplasmic Foci 0.065061 0.041798 0.034674 0.020537 0.044759 0.030756 0.030874 0.023610 0.045125 0.037642 0.034646 0.021984
Eisosomes 0.000974 0.000124 0.000103 0.000280 0.000164 0.000133 0.000715 0.000405 0.000179 0.000127 0.000107 0.000339
Endoplasmic Reticulum 0.000514 0.000274 0.001545 0.001926 0.000429 0.000420 0.010292 0.001784 0.000430 0.000329 0.001609 0.001859
Endosome 0.010612 0.056988 0.063373 0.027107 0.096261 0.055771 0.001499 0.027546 0.094717 0.056530 0.062918 0.027314
Golgi 0.058553 0.075641 0.075648 0.148233 0.077256 0.059288 0.000358 0.120898 0.076919 0.069486 0.075094 0.135367
Lipid Particles 0.022201 0.007501 0.007747 0.002478 0.021571 0.008930 0.000510 0.001219 0.021582 0.008039 0.007694 0.001885
Mitochondria 0.812447 0.747567 0.659471 0.674729 0.655899 0.775429 0.002400 0.704098 0.658721 0.758054 0.654637 0.688553
Mitotic Spindle 0.000563 0.005492 0.023309 0.016275 0.002513 0.004770 0.000203 0.014987 0.002478 0.005220 0.023139 0.015669
None 0.000882 0.000178 0.000748 0.000875 0.000224 0.000470 0.148580 0.000558 0.000236 0.000288 0.001836 0.000726
Nuclear Periphery 0.000314 0.000092 0.002436 0.000375 0.000050 0.000395 0.000388 0.000180 0.000054 0.000206 0.002421 0.000283
Nuclear Periphery Foci 0.000317 0.000131 0.002083 0.000434 0.000279 0.000571 0.019703 0.000178 0.000280 0.000297 0.002213 0.000313
Nucleolus 0.000063 0.000140 0.000742 0.000093 0.000907 0.000605 0.005568 0.000070 0.000892 0.000315 0.000778 0.000082
Nucleus 0.000041 0.000162 0.001478 0.000097 0.000385 0.000223 0.396926 0.000070 0.000379 0.000185 0.004388 0.000084
Peroxisomes 0.017261 0.043073 0.012112 0.012847 0.076102 0.025969 0.000186 0.021606 0.075041 0.036635 0.012024 0.016970
Vacuole 0.001315 0.002238 0.041592 0.017709 0.001615 0.008820 0.012513 0.009055 0.001610 0.004715 0.041378 0.013636
Vacuole Periphery 0.003351 0.003177 0.044029 0.028802 0.002683 0.009644 0.000305 0.010522 0.002695 0.005611 0.043707 0.020197

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.44 -0.16 -4.88 -3.62 -4.97 NaN NaN NaN NaN NaN 1.39 4.96 -6.16 -6.89 -8.26
Bud Neck -0.36 -5.59 -2.85 -2.79 3.17 NaN NaN NaN NaN NaN 1.71 -3.87 -0.21 -2.49 3.95
Bud Site -5.60 -10.52 -5.44 -4.22 -0.70 NaN NaN NaN NaN NaN -0.33 -6.28 -5.73 -5.89 -1.18
Cell Periphery 0.60 -2.45 -3.78 -4.50 -1.28 NaN NaN NaN NaN NaN 4.87 -1.64 -4.02 -6.79 -0.13
Cytoplasm -5.79 -5.48 -4.17 -1.98 2.17 NaN NaN NaN NaN NaN -0.57 -4.34 -2.93 -2.85 3.09
Cytoplasmic Foci 0.78 1.01 1.49 7.65 4.96 NaN NaN NaN NaN NaN 2.92 3.50 8.47 7.41 4.95
Eisosomes 1.39 1.42 1.13 -5.24 -5.90 NaN NaN NaN NaN NaN 3.56 4.56 -4.83 -6.89 -7.40
Endoplasmic Reticulum 1.21 -3.87 -3.09 -3.81 -0.79 NaN NaN NaN NaN NaN 1.31 -5.51 -3.84 -4.13 -0.58
Endosome -8.22 -8.96 -2.93 9.28 9.95 NaN NaN NaN NaN NaN 8.48 6.36 15.25 11.97 10.93
Golgi -0.65 -0.64 -3.22 -7.19 -7.01 NaN NaN NaN NaN NaN 1.85 0.39 -7.93 -9.55 -8.01
Lipid Particles 0.99 0.97 1.33 4.06 4.20 NaN NaN NaN NaN NaN 5.52 5.46 8.31 6.83 5.26
Mitochondria 0.97 2.30 2.02 4.49 -0.94 NaN NaN NaN NaN NaN -9.03 0.38 -2.11 5.95 -2.56
Mitotic Spindle -7.48 -11.39 -5.57 -3.76 2.11 NaN NaN NaN NaN NaN -3.17 -9.93 -6.61 -5.37 2.85
None 2.44 0.41 0.03 -10.53 -1.26 NaN NaN NaN NaN NaN -0.58 -2.77 -7.97 -9.61 1.95
Nuclear Periphery 1.29 -4.33 -0.34 -4.28 4.45 NaN NaN NaN NaN NaN -2.66 -5.19 -6.33 -1.15 4.67
Nuclear Periphery Foci 0.99 -4.44 -0.53 -2.27 4.37 NaN NaN NaN NaN NaN 0.24 -5.09 0.10 -0.16 5.17
Nucleolus -0.90 -4.78 -0.63 0.59 4.69 NaN NaN NaN NaN NaN 1.61 0.35 2.28 1.94 5.05
Nucleus -0.99 -2.85 -1.34 0.51 2.73 NaN NaN NaN NaN NaN 1.04 -3.24 1.65 1.23 3.54
Peroxisomes -1.61 0.32 0.27 8.06 -0.24 NaN NaN NaN NaN NaN 8.24 14.26 12.24 6.87 -2.01
Vacuole -1.63 -18.47 -6.19 -5.96 7.12 NaN NaN NaN NaN NaN -7.53 -18.91 -7.70 -5.57 10.59
Vacuole Periphery 0.14 -16.03 -7.22 -7.73 3.81 NaN NaN NaN NaN NaN -6.93 -18.58 -9.18 -7.52 8.07
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Componentsactinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of the ubiqunol-cytochrome c oxidoreductase complex; this complex comprises part of the mitochondrial respiratory chain; members include Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Qcr10

Qcr10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Qcr10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available