Standard name | |
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Human Ortholog | |
Description | tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD1 has a paralog, TCD2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 2 | 2 | 6 | 5 | 3 | 1 | 3 | 7 | 3 | 5 | 12 |
Bud | 0 | 0 | 7 | 11 | 4 | 14 | 24 | 16 | 22 | 29 | 21 | 23 | 1 | 1 | 0 | 1 | 6 | 5 | 6 | 3 | 4 | 4 | 17 | 13 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 3 | 3 |
Bud Site | 3 | 2 | 1 | 0 | 1 | 4 | 9 | 5 | 2 | 11 | 9 | 19 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 7 | 2 | 12 | 11 | 6 | 4 | 4 | 0 | 4 | 7 | 6 | 6 | 12 | 20 | 9 | 23 | 16 | 18 | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 10 | 7 | 24 | 35 | 39 | 32 | 47 | 60 | 43 | 57 | 40 | 48 | 52 | 80 | 78 | 39 | 20 | 16 | 1 | 0 | 0 | 13 | 12 | 13 |
Endoplasmic Reticulum | 0 | 2 | 0 | 1 | 2 | 4 | 1 | 0 | 1 | 3 | 3 | 1 | 0 | 4 | 2 | 7 | 7 | 3 | 1 | 1 | 0 | 1 | 5 | 2 |
Endosome | 11 | 4 | 36 | 13 | 13 | 4 | 2 | 15 | 1 | 1 | 1 | 2 | 12 | 38 | 22 | 1 | 3 | 1 | 7 | 6 | 16 | 16 | 17 | 19 |
Golgi | 0 | 6 | 9 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 81 | 142 | 114 | 88 | 84 | 62 | 7 | 9 | 15 | 14 | 30 | 34 |
Mitochondria | 208 | 164 | 354 | 314 | 341 | 489 | 704 | 672 | 539 | 625 | 619 | 645 | 187 | 157 | 154 | 17 | 14 | 13 | 181 | 140 | 356 | 229 | 339 | 371 |
Nucleus | 0 | 1 | 4 | 0 | 3 | 4 | 10 | 17 | 13 | 10 | 2 | 12 | 2 | 1 | 0 | 6 | 2 | 2 | 0 | 0 | 1 | 6 | 6 | 8 |
Nuclear Periphery | 1 | 4 | 9 | 3 | 7 | 33 | 39 | 22 | 22 | 16 | 21 | 11 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 0 | 7 | 2 | 1 | 4 |
Nucleolus | 11 | 4 | 9 | 7 | 14 | 27 | 45 | 41 | 23 | 21 | 22 | 16 | 4 | 1 | 2 | 1 | 3 | 3 | 1 | 0 | 1 | 0 | 3 | 3 |
Peroxisomes | 8 | 3 | 33 | 18 | 9 | 3 | 0 | 9 | 5 | 0 | 4 | 3 | 6 | 7 | 22 | 3 | 2 | 7 | 6 | 2 | 24 | 4 | 3 | 9 |
SpindlePole | 4 | 1 | 11 | 4 | 12 | 8 | 14 | 30 | 18 | 9 | 17 | 22 | 0 | 7 | 2 | 1 | 2 | 2 | 2 | 1 | 6 | 2 | 8 | 9 |
Vac/Vac Membrane | 27 | 28 | 45 | 24 | 51 | 41 | 38 | 47 | 44 | 37 | 43 | 42 | 68 | 153 | 62 | 36 | 55 | 42 | 7 | 13 | 3 | 24 | 50 | 46 |
Unique Cell Count | 232 | 181 | 428 | 368 | 409 | 553 | 766 | 784 | 622 | 678 | 656 | 704 | 320 | 444 | 343 | 190 | 180 | 155 | 233 | 186 | 453 | 333 | 513 | 563 |
Labelled Cell Count | 290 | 228 | 556 | 445 | 504 | 667 | 938 | 934 | 737 | 826 | 808 | 850 | 436 | 614 | 471 | 232 | 221 | 179 | 233 | 186 | 453 | 333 | 513 | 563 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 8.2 | 7.1 | 6.5 | 6.1 | 4.9 | 4.6 | 4.6 | 4.3 | 4.0 | 3.7 | 3.6 | 8.5 | 8.4 | 7.8 | 11.1 | 10.7 | 10.4 | 8.7 | 9.1 | 9.5 |
Std Deviation (1e-4) | 1.8 | 2.7 | 2.3 | 2.4 | 2.2 | 1.7 | 1.6 | 1.8 | 1.7 | 1.6 | 1.2 | 1.2 | 2.2 | 2.3 | 2.1 | 2.4 | 2.3 | 2.1 | 2.5 | 2.2 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.22 | -0.53 | -0.63 | -0.64 | -0.72 | -0.82 | -0.95 | -0.97 | 0.26 | 0.25 | 0.14 | 0.64 | 0.58 | 0.54 | 0.28 | 0.36 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1.3 | -0.8 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.2 | -1.3 | -2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 1.3 | -0.2 | 4.6 | 3.3 | 4.3 |
Cytoplasm | 2.1 | 2.2 | 0.1 | 0.4 | 1.3 | 0.9 | 1.7 | 0.3 | 0.8 | 4.8 | 5.7 | 7.0 | 5.7 | 2.4 | 2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -2.9 | -3.2 | -6.0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.6 | 0.1 | -1.0 | -3.8 | -3.1 | -3.4 |
Golgi | -1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9.7 | 11.7 | 11.7 | 13.9 | 13.9 | 12.4 |
Mitochondria | 1.0 | 0.3 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | -7.3 | -14.2 | -11.0 | -17.3 | -17.3 | -16.4 |
Nucleus | 0 | 0 | 0 | 0.6 | 1.6 | 1.5 | 0.8 | 0 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.5 | -0.4 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.3 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.2 | 1.2 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | -0.9 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -1.6 | -3.6 | -5.9 | 0 | 0 | 0 | 0 | 0 | 0 | -3.6 | -4.3 | -0.7 | -3.0 | -3.2 | -1.3 |
SpindlePole | -1.5 | 0.3 | -1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | -2.1 | 0 | 0 | 0 |
Vacuole | -2.0 | 0.9 | -1.7 | -3.6 | -2.8 | -2.0 | -3.1 | -2.3 | -2.8 | 4.1 | 8.4 | 3.0 | 2.9 | 6.1 | 5.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1855 | 2.5045 | 2.3628 | 2.9422 | 2.0491 | 2.7456 | 1.749 | 1.2206 | 1.2988 | 1.6564 | 1.0921 | 1.2733 | 0.1648 | 0.6569 | 0.6796 | 1.0189 | 0.4683 | 0.5512 |
Actin | 0.0219 | 0.0093 | 0.0407 | 0.0197 | 0.0252 | 0.0194 | 0.1102 | 0.0238 | 0.0408 | 0.0713 | 0.062 | 0.0237 | 0.0272 | 0.0191 | 0.0052 | 0.0055 | 0.0062 | 0.0201 |
Bud | 0.0013 | 0.0074 | 0.007 | 0.006 | 0.0045 | 0.0034 | 0.0025 | 0.0105 | 0.0087 | 0.0013 | 0.0079 | 0.0023 | 0.0031 | 0.0076 | 0.0021 | 0.0023 | 0.0049 | 0.001 |
Bud Neck | 0.002 | 0.0082 | 0.0034 | 0.0007 | 0.0012 | 0.005 | 0.0023 | 0.0026 | 0.0025 | 0.0009 | 0.0021 | 0.0049 | 0.0076 | 0.0011 | 0.0014 | 0.0008 | 0.0045 | 0.0055 |
Bud Periphery | 0.0037 | 0.0227 | 0.019 | 0.033 | 0.0428 | 0.0204 | 0.0047 | 0.014 | 0.0247 | 0.0032 | 0.0462 | 0.0111 | 0.0108 | 0.0203 | 0.0079 | 0.013 | 0.0174 | 0.004 |
Bud Site | 0.0064 | 0.0841 | 0.0286 | 0.0045 | 0.0014 | 0.0018 | 0.0111 | 0.094 | 0.0251 | 0.0021 | 0.0034 | 0.0013 | 0.0135 | 0.0265 | 0.0121 | 0.0017 | 0.0026 | 0.0017 |
Cell Periphery | 0.0017 | 0.0045 | 0.0071 | 0.0083 | 0.0027 | 0.003 | 0.0011 | 0.0034 | 0.0016 | 0.0009 | 0.0019 | 0.0012 | 0.001 | 0.0011 | 0.0021 | 0.0021 | 0.0011 | 0.0016 |
Cytoplasm | 0.0295 | 0.0411 | 0.0337 | 0.0025 | 0.0305 | 0.0639 | 0.0329 | 0.0243 | 0.0343 | 0.0051 | 0.0399 | 0.0577 | 0.0212 | 0.0303 | 0.0347 | 0.026 | 0.016 | 0.032 |
Cytoplasmic Foci | 0.0595 | 0.0562 | 0.0492 | 0.0537 | 0.0645 | 0.0484 | 0.0863 | 0.0431 | 0.0494 | 0.0372 | 0.0766 | 0.0495 | 0.0769 | 0.0757 | 0.0503 | 0.0308 | 0.0543 | 0.0641 |
Eisosomes | 0.0016 | 0.0009 | 0.0015 | 0.0004 | 0.0004 | 0.0007 | 0.0016 | 0.0019 | 0.0007 | 0.0007 | 0.0009 | 0.0006 | 0.0007 | 0.0007 | 0.0006 | 0.0005 | 0.0002 | 0.0003 |
Endoplasmic Reticulum | 0.0083 | 0.0027 | 0.0024 | 0.0006 | 0.0015 | 0.0021 | 0.0113 | 0.0041 | 0.0024 | 0.0012 | 0.0049 | 0.0047 | 0.0045 | 0.0014 | 0.0034 | 0.0088 | 0.0026 | 0.0013 |
Endosome | 0.0785 | 0.1135 | 0.0586 | 0.0203 | 0.0495 | 0.0552 | 0.098 | 0.1053 | 0.0556 | 0.0572 | 0.1053 | 0.0734 | 0.1456 | 0.0968 | 0.0937 | 0.0617 | 0.0414 | 0.0628 |
Golgi | 0.0415 | 0.0425 | 0.0324 | 0.02 | 0.033 | 0.0292 | 0.0412 | 0.0358 | 0.0298 | 0.0396 | 0.04 | 0.0552 | 0.0494 | 0.0448 | 0.0381 | 0.031 | 0.032 | 0.0429 |
Lipid Particles | 0.0249 | 0.011 | 0.0116 | 0.0244 | 0.0062 | 0.013 | 0.043 | 0.0141 | 0.0101 | 0.0135 | 0.0134 | 0.0274 | 0.032 | 0.0074 | 0.011 | 0.0016 | 0.0055 | 0.0094 |
Mitochondria | 0.4541 | 0.4364 | 0.5573 | 0.6545 | 0.6072 | 0.6155 | 0.3863 | 0.5063 | 0.5761 | 0.7221 | 0.4791 | 0.5504 | 0.4478 | 0.5138 | 0.6214 | 0.7272 | 0.5277 | 0.6563 |
None | 0.1872 | 0.0704 | 0.1129 | 0.0649 | 0.091 | 0.0719 | 0.0783 | 0.0554 | 0.0968 | 0.0065 | 0.0207 | 0.0973 | 0.0833 | 0.0897 | 0.067 | 0.0606 | 0.1561 | 0.0587 |
Nuclear Periphery | 0.0226 | 0.001 | 0.0006 | 0.0003 | 0.0004 | 0.0008 | 0.0133 | 0.0016 | 0.0015 | 0.0011 | 0.0074 | 0.0032 | 0.0108 | 0.0004 | 0.0013 | 0.0017 | 0.0217 | 0.0003 |
Nucleolus | 0.0013 | 0.0042 | 0.0014 | 0.0041 | 0.0003 | 0.0006 | 0.0014 | 0.0025 | 0.0012 | 0.0005 | 0.0014 | 0.0004 | 0.0018 | 0.0016 | 0.0009 | 0.0004 | 0.0039 | 0.0003 |
Nucleus | 0.0113 | 0.0016 | 0.0009 | 0.0013 | 0.0006 | 0.0006 | 0.0017 | 0.002 | 0.0029 | 0.0009 | 0.0019 | 0.0012 | 0.0021 | 0.0012 | 0.0011 | 0.0007 | 0.0053 | 0.0005 |
Peroxisomes | 0.0256 | 0.0348 | 0.0159 | 0.056 | 0.0278 | 0.0307 | 0.0448 | 0.0236 | 0.0228 | 0.0251 | 0.049 | 0.0218 | 0.0344 | 0.0488 | 0.0273 | 0.0136 | 0.0644 | 0.0302 |
Punctate Nuclear | 0.0043 | 0.001 | 0.0009 | 0.0006 | 0.0005 | 0.0015 | 0.0142 | 0.0016 | 0.0038 | 0.0011 | 0.0087 | 0.0037 | 0.003 | 0.0013 | 0.001 | 0.0003 | 0.0035 | 0.0005 |
Vacuole | 0.0079 | 0.0353 | 0.0113 | 0.0184 | 0.0059 | 0.0087 | 0.0086 | 0.0227 | 0.006 | 0.0044 | 0.0196 | 0.0051 | 0.0138 | 0.0073 | 0.0115 | 0.0068 | 0.0216 | 0.0039 |
Vacuole Periphery | 0.0049 | 0.011 | 0.0035 | 0.0058 | 0.0031 | 0.0043 | 0.0054 | 0.0074 | 0.0033 | 0.0044 | 0.0077 | 0.004 | 0.0096 | 0.003 | 0.0059 | 0.003 | 0.007 | 0.0027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.1008 | 29.8207 | 15.3195 | 23.9759 | 18.853 | 14.5329 | 20.4877 | 16.6977 | 21.1092 | 23.4195 |
Translational Efficiency | 0.9625 | 0.6809 | 1.3696 | 0.8511 | 1.0208 | 1.2635 | 0.9838 | 1.0045 | 0.8284 | 0.7876 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2178 | 626 | 2704 | 1730 | 1946 | 1649 | 169 | 1208 | 4124 | 2275 | 2873 | 2938 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 744.24 | 836.16 | 1082.95 | 1105.72 | 795.72 | 847.25 | 1144.66 | 1232.83 | 768.53 | 844.20 | 1086.58 | 1157.98 |
Standard Deviation | 138.02 | 169.59 | 206.47 | 210.04 | 152.54 | 166.97 | 200.47 | 238.41 | 147.31 | 167.77 | 206.63 | 230.78 |
Intensity Change Log 2 | — | 0.168011 | 0.541127 | 0.571146 | — | 0.090527 | 0.524586 | 0.631641 | — | 0.128494 | 0.532604 | 0.602721 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000909 | 0.002585 | 0.001073 | 0.006855 | 0.001175 | 0.001357 | 0.002836 | 0.004065 | 0.001035 | 0.001695 | 0.001177 | 0.005708 |
Bud Neck | 0.013883 | 0.024223 | 0.006255 | 0.003514 | 0.013042 | 0.015253 | 0.022149 | 0.005110 | 0.013486 | 0.017721 | 0.007190 | 0.004170 |
Bud Site | 0.062155 | 0.068486 | 0.027773 | 0.062685 | 0.049167 | 0.042215 | 0.050670 | 0.072591 | 0.056026 | 0.049444 | 0.029120 | 0.066758 |
Cell Periphery | 0.002514 | 0.000745 | 0.000560 | 0.001723 | 0.001358 | 0.001088 | 0.000661 | 0.007988 | 0.001969 | 0.000994 | 0.000566 | 0.004299 |
Cytoplasm | 0.035743 | 0.042099 | 0.012193 | 0.016670 | 0.044014 | 0.041929 | 0.020418 | 0.024666 | 0.039645 | 0.041976 | 0.012677 | 0.019958 |
Cytoplasmic Foci | 0.037467 | 0.092050 | 0.010175 | 0.012325 | 0.038026 | 0.049796 | 0.068841 | 0.002242 | 0.037731 | 0.061422 | 0.013626 | 0.008179 |
Eisosomes | 0.000096 | 0.000128 | 0.000045 | 0.000097 | 0.000089 | 0.000100 | 0.000140 | 0.000079 | 0.000093 | 0.000107 | 0.000051 | 0.000090 |
Endoplasmic Reticulum | 0.001514 | 0.000900 | 0.001037 | 0.000941 | 0.001198 | 0.001037 | 0.003529 | 0.000671 | 0.001365 | 0.000999 | 0.001184 | 0.000830 |
Endosome | 0.039042 | 0.075406 | 0.026946 | 0.018233 | 0.048245 | 0.068022 | 0.061001 | 0.005875 | 0.043385 | 0.070054 | 0.028949 | 0.013152 |
Golgi | 0.032142 | 0.058222 | 0.026398 | 0.049314 | 0.030794 | 0.041978 | 0.032925 | 0.013530 | 0.031506 | 0.046447 | 0.026782 | 0.034601 |
Lipid Particles | 0.004053 | 0.005043 | 0.001032 | 0.000347 | 0.003930 | 0.005839 | 0.010183 | 0.000022 | 0.003995 | 0.005620 | 0.001571 | 0.000214 |
Mitochondria | 0.690038* | 0.499428* | 0.715237* | 0.737238* | 0.670264* | 0.608011* | 0.454784* | 0.824839* | 0.680707* | 0.578133* | 0.699916* | 0.773256* |
Mitotic Spindle | 0.010428 | 0.020403 | 0.035879 | 0.019053 | 0.013716 | 0.020400 | 0.066691 | 0.010694 | 0.011979 | 0.020401 | 0.037692 | 0.015616 |
None | 0.002977 | 0.000788 | 0.001209 | 0.000945 | 0.002578 | 0.002797 | 0.001885 | 0.000608 | 0.002788 | 0.002245 | 0.001248 | 0.000807 |
Nuclear Periphery | 0.000181 | 0.000555 | 0.001337 | 0.000236 | 0.000194 | 0.000285 | 0.005527 | 0.000011 | 0.000187 | 0.000359 | 0.001583 | 0.000143 |
Nuclear Periphery Foci | 0.000467 | 0.001558 | 0.000704 | 0.000222 | 0.000404 | 0.000548 | 0.003772 | 0.000009 | 0.000437 | 0.000826 | 0.000884 | 0.000135 |
Nucleolus | 0.000743 | 0.001526 | 0.001036 | 0.000211 | 0.000996 | 0.002982 | 0.003356 | 0.000018 | 0.000862 | 0.002581 | 0.001172 | 0.000131 |
Nucleus | 0.003546 | 0.010431 | 0.005778 | 0.001207 | 0.004727 | 0.009489 | 0.038213 | 0.000269 | 0.004103 | 0.009748 | 0.007686 | 0.000821 |
Peroxisomes | 0.015858 | 0.046439 | 0.004620 | 0.011246 | 0.018719 | 0.019639 | 0.050437 | 0.004020 | 0.017208 | 0.027014 | 0.007315 | 0.008275 |
Vacuole | 0.024638 | 0.034194 | 0.061977 | 0.027331 | 0.034508 | 0.041697 | 0.053639 | 0.013032 | 0.029295 | 0.039633 | 0.061486 | 0.021452 |
Vacuole Periphery | 0.021606 | 0.014793 | 0.058735 | 0.029608 | 0.022857 | 0.025537 | 0.048343 | 0.009663 | 0.022196 | 0.022581 | 0.058124 | 0.021407 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.09 | -0.92 | -6.55 | -4.12 | -6.30 | -0.50 | -2.86 | -3.15 | -2.84 | -1.17 | -2.26 | -0.85 | -7.13 | -5.74 | -6.78 |
Bud Neck | -3.24 | 6.12 | 9.20 | 6.83 | 4.58 | -1.23 | -2.07 | 5.54 | 5.94 | 3.92 | -2.77 | 6.35 | 10.59 | 9.71 | 4.44 |
Bud Site | -0.89 | 9.18 | -0.40 | 0.56 | -8.27 | 1.69 | -0.17 | -4.11 | -5.36 | -1.95 | 1.91 | 9.34 | -3.08 | -4.48 | -10.72 |
Cell Periphery | 6.63 | 7.73 | 2.20 | -3.38 | -4.19 | 1.67 | 4.00 | -5.66 | -5.90 | -6.25 | 5.96 | 9.08 | -4.42 | -6.42 | -7.29 |
Cytoplasm | -1.42 | 8.45 | 5.97 | 5.06 | -2.14 | 0.35 | 3.72 | 4.88 | 4.37 | -0.69 | -0.96 | 11.63 | 7.84 | 7.31 | -3.66 |
Cytoplasmic Foci | -8.51 | 11.89 | 10.49 | 13.02 | -1.88 | -3.46 | -3.30 | 16.66 | 17.43 | 7.31 | -7.90 | 13.66 | 18.00 | 19.94 | 5.04 |
Eisosomes | -3.54 | 10.94 | -0.20 | 3.21 | -9.29 | -1.81 | -2.80 | 1.44 | 2.90 | 3.27 | -3.01 | 11.52 | 0.60 | 3.21 | -8.72 |
Endoplasmic Reticulum | 2.34 | 1.90 | 2.29 | -0.40 | 0.65 | 0.75 | -1.72 | 1.81 | 1.51 | 2.11 | 2.22 | 0.78 | 2.86 | 1.08 | 2.20 |
Endosome | -7.17 | 5.62 | 10.06 | 11.72 | 5.24 | -5.43 | -1.68 | 18.47 | 21.04 | 7.64 | -9.39 | 7.70 | 19.36 | 22.20 | 10.95 |
Golgi | -5.52 | 2.56 | -4.95 | 1.70 | -6.85 | -3.91 | -0.42 | 7.68 | 10.90 | 4.04 | -6.29 | 2.53 | -1.30 | 4.36 | -3.28 |
Lipid Particles | -0.77 | 4.46 | 5.52 | 4.20 | 3.75 | -2.28 | -2.18 | 7.11 | 8.92 | 3.61 | -2.31 | 4.97 | 8.60 | 9.54 | 5.89 |
Mitochondria | 11.02 | -2.15 | -3.77 | -13.28 | -2.00 | 5.01 | 7.13 | -13.13 | -17.43 | -12.26 | 10.46 | -1.86 | -10.88 | -19.19 | -8.41 |
Mitotic Spindle | -3.38 | -11.75 | -4.92 | 0.26 | 6.41 | -3.34 | -4.92 | 1.90 | 4.60 | 5.19 | -5.26 | -12.18 | -3.04 | 2.47 | 9.59 |
None | 5.18 | 4.06 | 4.54 | -0.73 | 1.19 | -0.42 | 0.71 | 6.22 | 4.33 | 1.32 | 1.13 | 5.12 | 6.99 | 3.78 | 2.48 |
Nuclear Periphery | -1.44 | -5.59 | -0.78 | 1.20 | 5.17 | -1.83 | -2.88 | 5.13 | 7.77 | 2.98 | -2.17 | -6.28 | 0.96 | 2.58 | 6.43 |
Nuclear Periphery Foci | -0.95 | -1.25 | 1.48 | 1.18 | 6.32 | -1.29 | -2.88 | 4.56 | 7.21 | 3.23 | -1.17 | -3.28 | 3.11 | 2.20 | 7.79 |
Nucleolus | -0.88 | -1.33 | 5.23 | 1.49 | 3.97 | -2.98 | -2.17 | 4.47 | 4.68 | 3.17 | -3.24 | -1.35 | 6.24 | 4.71 | 5.14 |
Nucleus | -2.74 | -2.64 | 2.52 | 3.55 | 4.52 | -3.44 | -3.06 | 6.49 | 7.39 | 3.48 | -4.72 | -3.57 | 5.55 | 7.46 | 6.32 |
Peroxisomes | -7.14 | 8.40 | 2.61 | 8.21 | -4.86 | -0.43 | -3.42 | 8.35 | 8.88 | 5.04 | -5.29 | 8.14 | 6.98 | 10.46 | -0.88 |
Vacuole | -2.91 | -15.18 | -1.95 | 1.49 | 11.50 | -2.62 | -2.34 | 8.85 | 11.03 | 5.02 | -5.11 | -13.79 | 3.97 | 7.90 | 15.93 |
Vacuole Periphery | 3.93 | -14.57 | -3.47 | -6.46 | 9.92 | -1.31 | -3.09 | 6.89 | 7.92 | 4.70 | -0.23 | -15.35 | 0.55 | 0.70 | 14.71 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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