Standard name | |
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Human Ortholog | |
Description | Lanosterol 14-alpha-demethylase; catalyzes C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in ergosterol biosynthesis pathway; transcriptionally down-regulated when ergosterol is in excess; member of cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p; human CYP51A1 functionally complements the lethality of the erg11 null mutation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 11 | 19 | 17 | 9 | 17 | 31 | 35 | – | 63 | 34 | 36 | 8 | 8 | 4 | 16 | 15 | 14 | 2 | 5 | 8 | 2 | 7 | 7 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 9 | – | 6 | 2 | 1 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 6 | 1 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 32 | 52 | 21 | 23 | 19 | 15 | 35 | – | 53 | 46 | 51 | 41 | 41 | 67 | 96 | 68 | 69 | 0 | 3 | 15 | 6 | 13 | 17 |
Cytoplasm | 12 | 56 | 22 | 18 | 27 | 28 | 34 | 87 | – | 71 | 41 | 47 | 13 | 13 | 29 | 63 | 63 | 61 | 5 | 22 | 4 | 4 | 3 | 4 |
Endoplasmic Reticulum | 122 | 397 | 385 | 236 | 237 | 344 | 317 | 530 | – | 360 | 252 | 219 | 205 | 205 | 298 | 3 | 9 | 4 | 173 | 449 | 407 | 215 | 333 | 316 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 |
Golgi | 4 | 4 | 60 | 42 | 26 | 4 | 10 | 10 | – | 6 | 1 | 4 | 1 | 1 | 2 | 42 | 72 | 75 | 2 | 0 | 1 | 2 | 5 | 6 |
Mitochondria | 1 | 0 | 0 | 11 | 1 | 18 | 39 | 29 | – | 51 | 51 | 40 | 0 | 0 | 0 | 24 | 17 | 12 | 2 | 2 | 2 | 9 | 9 | 9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | – | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 9 | 3 | 6 | 12 | 14 | 29 | – | 15 | 8 | 18 | 0 | 0 | 4 | 3 | 1 | 2 | 2 | 1 | 1 | 3 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 7 | 7 | 6 | 2 | 17 | 7 | 26 | 26 | – | 24 | 7 | 19 | 0 | 1 | 8 | 13 | 25 | 20 | 2 | 4 | 1 | 6 | 8 | 7 |
Unique Cell Count | 143 | 457 | 436 | 275 | 285 | 389 | 400 | 673 | 511 | 352 | 334 | 235 | 233 | 345 | 230 | 229 | 217 | 198 | 502 | 448 | 256 | 388 | 375 | |
Labelled Cell Count | 151 | 508 | 554 | 350 | 346 | 449 | 486 | 795 | 650 | 442 | 435 | 269 | 270 | 414 | 266 | 275 | 262 | 198 | 502 | 448 | 256 | 388 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 28.9 | 31.6 | 30.4 | 29.8 | 25.4 | 20.2 | 19.6 | 20.1 | – | 18.6 | 18.1 | 18.6 | 24.3 | 24.3 | 20.5 | 20.4 | 25.5 | 28.2 | 30.9 | 28.9 | 29.0 |
Std Deviation (1e-4) | 6.9 | 8.1 | 6.8 | 5.4 | 4.7 | 3.6 | 3.5 | 3.9 | – | 3.9 | 3.7 | 4.3 | 3.9 | 3.9 | 3.5 | 5.5 | 8.1 | 8.5 | 6.7 | 5.5 | 5.6 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.26 | -0.59 | -0.63 | -0.6 | – | -0.71 | -0.75 | -0.71 | -0.33 | -0.33 | -0.57 | -0.58 | -0.26 | -0.11 | 0.02 | -0.08 | -0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.1 | -0.8 | 0 | 2.1 | 0.6 | – | 4.3 | 3.0 | 3.4 | -0.6 | -0.6 | -2.6 | 1.4 | 1.2 | 1.2 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.8 | -1.7 | -3.6 | -4.3 | -4.1 | – | -0.8 | 0.5 | 1.4 | 2.0 | 2.0 | 2.9 | 8.8 | 5.7 | 6.2 |
Cytoplasm | 0.8 | 2.3 | 1.3 | 2.0 | 4.3 | – | 4.6 | 3.4 | 4.3 | 0.3 | 0.3 | 1.9 | 8.2 | 8.2 | 8.3 |
Endoplasmic Reticulum | -1.0 | -2.0 | 0.1 | -3.6 | -4.1 | – | -6.7 | -5.9 | -7.6 | -0.4 | -0.1 | -0.8 | -21.6 | -21.0 | -21.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.6 | -1.9 | -6.8 | -5.9 | -8.2 | – | -7.6 | -7.0 | -6.3 | -5.7 | -5.7 | -6.8 | 1.5 | 5.4 | 6.2 |
Mitochondria | 0 | 0 | 4.5 | 6.7 | 4.4 | – | 6.8 | 8.2 | 7.4 | 0 | 0 | 0 | 6.9 | 5.8 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0.9 | 1.3 | 2.0 | – | 0.9 | 0.2 | 2.5 | 0 | 0 | -1.0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.4 | 0.5 | 3.9 | 2.4 | – | 2.9 | 0.7 | 3.3 | 0 | 0 | 1.0 | 3.2 | 5.5 | 4.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 30.8618 | 26.9057 | 27.0583 | 27.1531 | 25.0291 | 27.4694 | 32.1926 | 28.5701 | 30.3703 | 29.2481 | 26.2161 | 27.3584 | 34.7827 | 28.9868 | 29.5821 | 28.9988 | 26.058 | 29.8109 |
Actin | 0.0002 | 0.0009 | 0.0014 | 0.0013 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0009 | 0.0011 | 0.0001 | 0.0005 | 0.0005 | 0.0005 |
Bud | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0002 | 0.001 | 0.0007 | 0.0042 | 0.002 | 0.001 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0013 | 0.0009 |
Bud Periphery | 0.0001 | 0.0004 | 0.0005 | 0.0024 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0 | 0.0018 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0048 | 0.0279 | 0.0207 | 0.0238 | 0.0104 | 0.0043 | 0.0022 | 0.0043 | 0.0024 | 0.0007 | 0.0013 | 0.0026 | 0.008 | 0.0185 | 0.0054 | 0.0048 | 0.0085 | 0.0038 |
Cytoplasm | 0.0276 | 0.0704 | 0.0344 | 0.0773 | 0.0174 | 0.0152 | 0.0018 | 0.0057 | 0.0031 | 0.0002 | 0.0054 | 0.0032 | 0.013 | 0.0378 | 0.0074 | 0.0062 | 0.0035 | 0.0099 |
Cytoplasmic Foci | 0.0001 | 0.001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.0002 | 0 | 0 | 0.0003 |
Eisosomes | 0 | 0.0008 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0.0017 | 0 | 0.0003 | 0.001 | 0 |
Endoplasmic Reticulum | 0.9126 | 0.8121 | 0.9051 | 0.8307 | 0.886 | 0.9468 | 0.9824 | 0.9737 | 0.9879 | 0.9966 | 0.9871 | 0.9891 | 0.895 | 0.9031 | 0.9724 | 0.9555 | 0.9704 | 0.9495 |
Endosome | 0.0006 | 0.0019 | 0.0015 | 0.0013 | 0.0011 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.001 | 0.0004 | 0.0003 | 0.0003 | 0.0008 |
Golgi | 0.0002 | 0.0017 | 0.0029 | 0.0009 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0016 | 0.0015 | 0.0002 | 0.0006 | 0.0012 | 0.0003 |
Lipid Particles | 0.005 | 0.0227 | 0.011 | 0.0031 | 0.0022 | 0.0029 | 0.0011 | 0.0069 | 0.0017 | 0.0001 | 0.0005 | 0.0013 | 0.001 | 0.0079 | 0.0019 | 0.0004 | 0.0006 | 0.0022 |
Mitochondria | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0067 | 0.0027 | 0 | 0.0001 | 0 | 0.0001 |
None | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0009 | 0 | 0.0002 | 0.0001 | 0.0004 |
Nuclear Periphery | 0.0359 | 0.0291 | 0.0102 | 0.035 | 0.0522 | 0.0208 | 0.0102 | 0.0043 | 0.0031 | 0.002 | 0.0027 | 0.0022 | 0.0592 | 0.0133 | 0.009 | 0.0265 | 0.0107 | 0.0257 |
Nucleolus | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0 |
Nucleus | 0.0059 | 0.0079 | 0.001 | 0.0038 | 0.0112 | 0.0032 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0041 | 0.0016 | 0.0002 | 0.0023 | 0.0003 | 0.0019 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0006 |
Vacuole | 0.0057 | 0.0182 | 0.0087 | 0.0132 | 0.0159 | 0.004 | 0.0015 | 0.0039 | 0.001 | 0.0003 | 0.0019 | 0.0012 | 0.0066 | 0.0049 | 0.0023 | 0.0013 | 0.0012 | 0.0027 |
Vacuole Periphery | 0.0006 | 0.0007 | 0.0005 | 0.0012 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0013 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 289.8774 | 364.7053 | 366.1966 | 430.9374 | 414.9044 | 224.297 | 347.7799 | 355.7894 | 349.0505 | 432.7325 |
Translational Efficiency | 0.8658 | 1.0356 | 1.0824 | 0.889 | 0.9218 | 0.862 | 0.9679 | 0.8091 | 1.0027 | 0.9181 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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