Standard name
Human Ortholog
Description Homoserine kinase; conserved protein required for threonine biosynthesis; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic filaments; expression is regulated by the GCN4-mediated general amino acid control pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.85 0.77 0.96 0.93 0.95 0.81 0.7 0.58 0.52 0.48 0.43 0.33 0.71 0.23 0.07 0.55 0.57 0.6 0.85 0.77 0.9 0.83 0.73 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0 0 0
Golgi 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.16 0.07 0.09 0.13 0.12 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.1 0.07 0.2 0.07 0 0 0.09 0 0 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0.06 0 0 0 0 0 0 0 0.07 0.08 0.1 0.18 0.4 0.52 0.26 0.2 0.17 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.18 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.21 0.25 0 0 0 0 0.06 0 0 0.09 0.11
Vac/Vac Membrane 0.05 0.08 0 0.07 0 0.15 0.21 0.3 0.3 0.3 0.24 0.37 0.05 0.05 0.05 0.06 0.11 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 0 0 0 1 4 5 8 7 7 14 1 2 0 1 5 2 0 3 1 0 2 1
Bud 4 2 0 1 2 0 6 3 12 6 13 15 2 0 0 1 0 3 0 7 2 3 5 10
Bud Neck 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 3 2 0 1 4 6
Bud Site 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 4 1 2 1 6 7 10 11 10 6 1 0 0 1 2 0 0 0 0 0 0 0
Cytoplasm 131 131 217 152 257 211 219 176 155 117 97 94 70 22 3 113 152 160 154 160 206 89 170 173
Endoplasmic Reticulum 0 4 1 0 9 4 3 9 7 4 4 2 1 0 0 6 5 4 2 0 2 0 1 4
Endosome 1 0 0 0 0 3 2 4 5 6 5 12 1 2 5 0 0 1 2 4 1 0 4 4
Golgi 1 11 1 0 0 1 6 4 5 5 3 7 2 16 3 19 34 32 1 3 0 1 1 0
Mitochondria 4 2 0 0 0 1 10 9 29 18 44 19 1 1 4 7 4 16 1 0 1 0 0 1
Nucleus 2 0 2 1 0 1 6 0 0 1 4 2 0 0 0 0 0 0 1 0 2 1 1 2
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 9 10 4 0 0 5 2 12 13 17 18 28 18 39 23 54 52 46 1 4 0 1 2 3
Peroxisomes 0 0 0 0 0 0 1 1 0 1 0 5 3 6 8 3 1 0 0 0 0 0 4 2
SpindlePole 1 1 1 0 0 2 0 4 3 9 3 20 5 20 11 8 11 8 7 11 6 2 21 27
Vac/Vac Membrane 8 13 3 11 6 40 65 90 91 72 55 103 5 5 2 13 28 21 0 5 2 0 5 4
Unique Cell Count 155 170 227 164 271 262 315 301 299 244 225 281 98 97 44 205 265 268 183 209 230 108 233 250
Labelled Cell Count 163 179 233 166 276 270 330 325 338 277 264 330 110 113 59 226 294 293 183 209 230 108 233 250


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 32.4 43.6 32.2 28.9 26.6 20.8 17.9 18.8 16.5 16.7 14.6 15.9 33.7 35.0 35.1 45.7 47.8 46.8 34.4 34.0 34.3
Std Deviation (1e-4) 8.0 9.4 7.2 7.4 5.0 4.8 4.3 3.8 4.2 4.1 4.4 4.1 8.2 8.4 12.6 15.2 14.1 14.6 9.2 8.9 7.8
Intensity Change (Log2) – – – -0.16 -0.27 -0.63 -0.85 -0.77 -0.97 -0.95 -1.14 -1.02 0.06 0.12 0.12 0.5 0.57 0.54 0.1 0.08 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.4 0 0 0 0 0 0
Bud 0 0 0 0 0 3.1 0 3.7 3.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1.1 1.7 1.6 0 0 0 0 0 0 0
Cytoplasm -1.2 -0.4 -5.0 -7.5 -9.7 -10.9 -11.4 -12.1 -14.3 -6.2 -13.7 -13.8 -9.9 -9.8 -9.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 5.9 0 4.4 5.3 5.1
Mitochondria 0 0 0 0 0 4.8 4.2 7.0 4.0 0 0 0 0 0 3.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1.5 1.7 2.7 3.1 3.8 5.5 9.3 0 7.5 6.2 5.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 3.8 0 6.8 0 0 2.7 0
Vacuole 2.8 0 5.4 6.7 8.5 8.6 8.4 7.3 9.7 0 0 0 2.8 4.2 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 27.0975 39.3788 31.3542 35.3176 26.8745 37.2329 28.6842 40.3807 32.9414 28.6885 30.4227 30.237 23.3333 36.856 35.6447 28.4563 34.5903 28.7473
Actin 0.0153 0.001 0.0057 0 0.0478 0.0004 0.0151 0.005 0.0092 0.0106 0.0004 0.0072 0.0105 0.0057 0.003 0.0265 0.0014 0.0007
Bud 0.0019 0.004 0.0018 0 0.0049 0.0005 0.0036 0.0103 0.0013 0.0008 0.0007 0.0038 0.0016 0.0228 0.001 0.0186 0.0026 0.0009
Bud Neck 0.0003 0.0174 0.0008 0 0.0009 0.0013 0.0029 0.0074 0.0032 0.0009 0.0005 0.0015 0.001 0.0027 0.0009 0.0035 0.0009 0.0008
Bud Periphery 0.0014 0.0012 0.0007 0 0.0025 0.0002 0.0015 0.0031 0.0004 0.0007 0.0001 0.0036 0.0017 0.0092 0.0007 0.0269 0.0008 0.0003
Bud Site 0.0018 0.0173 0.0067 0 0.0121 0.0001 0.0047 0.0285 0.0051 0.0088 0.0005 0.0029 0.0145 0.0517 0.0019 0.0118 0.0004 0.0007
Cell Periphery 0.0003 0.001 0.0002 0 0.0002 0 0.0002 0.0007 0.0002 0.0007 0.0038 0.0006 0.0007 0.0037 0.0008 0.0007 0.0009 0.0004
Cytoplasm 0.5064 0.7279 0.6917 0.9664 0.6794 0.5656 0.6978 0.7473 0.721 0.6615 0.7211 0.5284 0.5949 0.6751 0.7858 0.8042 0.7167 0.7923
Cytoplasmic Foci 0.3041 0.1207 0.1303 0 0.109 0.2637 0.1277 0.1021 0.1677 0.0304 0.0977 0.2145 0.1086 0.1386 0.1329 0.0077 0.158 0.0781
Eisosomes 0.0002 0.0001 0 0 0.0001 0 0.0002 0.0002 0.0002 0.0002 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0068 0.0002 0.0199 0 0.0012 0.004 0.0069 0.0001 0.0027 0.0031 0.0012 0.0022 0.0282 0.0002 0.0019 0.0018 0.0006 0.0037
Endosome 0.0199 0.0027 0.0191 0 0.0102 0.0076 0.0117 0.001 0.0093 0.0454 0.0018 0.0267 0.0333 0.0015 0.0015 0.0221 0.0026 0.0013
Golgi 0.0065 0.0008 0.0035 0 0.004 0.0009 0.0039 0.0005 0.0022 0.0145 0.0004 0.0156 0.008 0.0008 0.0005 0.0075 0.0006 0.0001
Lipid Particles 0.0131 0.0016 0.0153 0 0.009 0.0032 0.0049 0.0018 0.0091 0.0421 0.0188 0.0207 0.0071 0.0031 0.0015 0.001 0.0099 0.0029
Mitochondria 0.015 0.006 0.0081 0.0001 0.0063 0.0014 0.0165 0.0055 0.0034 0.0148 0.0346 0.0514 0.0037 0.0106 0.0042 0.0145 0.0087 0.003
None 0.0184 0.0019 0.0026 0.0001 0.0012 0.0006 0.005 0.001 0.011 0.0025 0.0059 0.007 0.0128 0.004 0.0004 0.0021 0.0038 0.0604
Nuclear Periphery 0.0137 0.0015 0.0121 0.0009 0.0212 0.0508 0.0209 0.0013 0.0046 0.0135 0.0076 0.0111 0.0442 0.0013 0.003 0.0025 0.0044 0.0044
Nucleolus 0.003 0.0464 0.0266 0 0.0014 0.0008 0.0043 0.0456 0.0103 0.0964 0.015 0.0439 0.0011 0.0245 0.0352 0.0007 0.0218 0.0312
Nucleus 0.0395 0.0275 0.0367 0.0324 0.0617 0.06 0.0325 0.0151 0.0126 0.0246 0.0271 0.0207 0.1014 0.0109 0.0143 0.0143 0.0189 0.0116
Peroxisomes 0.0143 0.0057 0.0033 0 0.0169 0.0035 0.0068 0.0031 0.0039 0.0052 0.0221 0.0135 0.0031 0.0035 0.0039 0.0024 0.0097 0.0019
Punctate Nuclear 0.0099 0.0068 0.0094 0 0.0022 0.0124 0.0293 0.0177 0.0204 0.0077 0.0085 0.0131 0.0139 0.0081 0.0025 0.0007 0.0033 0.001
Vacuole 0.0053 0.0057 0.0033 0 0.0051 0.0133 0.0022 0.0022 0.0015 0.0082 0.0274 0.0062 0.0068 0.0171 0.0021 0.0274 0.0254 0.0032
Vacuole Periphery 0.0032 0.0025 0.0022 0 0.0024 0.0096 0.0014 0.0005 0.0008 0.0073 0.0048 0.0054 0.0028 0.0048 0.0019 0.0031 0.0086 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 233.7286 247.0532 222.8513 203.4311 195.144 221.7909 240.2771 201.4992 214.2878 184.9452
Translational Efficiency 1.0589 1.05 1.1193 1.2648 1.0811 1.1983 1.146 1.2536 1.0987 1.1155

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Homoserine kinase; conserved protein required for threonine biosynthesis; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic filaments; expression is regulated by the GCN4-mediated general amino acid control pathway
Localization
Cell Percentages cytoplasm (78%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Thr1

Thr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Thr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available