Standard name
Human Ortholog
Description Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Cytoplasm 0.98 0.94 0.91 0.98 0.99 0.96 0.91 0.87 0.82 0.81 0.66 0.79 0.98 1.0 1.0 0.93 0.92 0.89 0.95 0.83 0.85 0.9 0.94 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0 0 0 0.09 0.24 0.19 0.38 0.26 0.52 0.29 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.13 0.11 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud 0 0 0 0 1 1 2 7 12 9 11 12 1 0 0 1 0 0 0 0 0 1 0 8
Bud Neck 0 0 0 0 1 0 1 3 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 0 7 3 0 5 1 8 3 3 1 2 2 4 12 10 0 0 0 0 0 0
Cytoplasm 295 47 32 188 332 255 337 420 346 343 202 288 252 283 304 194 197 159 286 62 52 135 315 329
Endoplasmic Reticulum 0 0 1 0 0 0 0 3 2 3 2 2 1 0 0 4 5 6 1 0 0 0 1 1
Endosome 0 0 0 0 0 0 0 0 1 3 0 1 0 0 0 0 0 0 2 2 0 1 2 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 1
Mitochondria 12 5 1 4 5 25 89 93 163 108 158 107 0 1 0 2 2 5 3 2 2 4 3 3
Nucleus 0 1 2 0 2 0 1 7 0 0 3 4 1 0 0 2 5 2 0 1 0 2 0 1
Nuclear Periphery 0 1 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 1 0 1 0 0 0 0 1 0 2 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 2 1 3 0 1 1 0 0 1 0 0 1 0 0 0 0 3
Vac/Vac Membrane 2 1 0 4 3 0 7 5 6 8 2 12 1 1 0 17 27 20 0 4 2 2 1 3
Unique Cell Count 301 50 35 191 336 266 371 482 424 421 304 365 257 284 305 209 213 179 303 75 62 151 335 366
Labelled Cell Count 311 55 37 197 351 285 439 546 534 487 384 431 260 287 307 225 248 202 303 75 62 151 335 366


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.2 4.8 5.4 5.6 4.5 4.2 4.6 3.9 4.2 3.7 4.1 6.1 6.4 6.2 8.0 8.9 9.0 5.7 6.0 6.7
Std Deviation (1e-4) 0.5 0.8 0.6 1.0 1.5 1.4 1.6 1.2 1.0 1.0 0.7 1.0 0.9 0.9 0.9 1.2 1.6 1.7 1.2 1.0 1.7
Intensity Change (Log2) – – – 0.16 0.21 -0.11 -0.19 -0.07 -0.3 -0.2 -0.39 -0.24 0.35 0.41 0.37 0.74 0.89 0.9 0.25 0.33 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 3.1 1.2 -0.1 -0.7 -1.5 -1.5 -3.0 -1.8 2.3 4.1 4.3 0.3 0.2 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9838 2.4724 2.3255 2.0785 1.756 2.2742 1.1713 2.3286 1.7449 2.2525 1.5755 1.833 0.2417 0.5718 0.4175 0.3607 0.271 0.208
Actin 0.0063 0.0006 0.0195 0.0477 0.0242 0.0011 0.0228 0.0019 0.0098 0.0233 0.0254 0.0085 0.028 0.0044 0.0026 0.0917 0.0124 0.0023
Bud 0.0005 0.0002 0.0005 0.0018 0.0072 0.0014 0.0005 0.0022 0.0012 0.0006 0.0015 0.0001 0.0003 0.0001 0.0003 0.0013 0.0002 0.0003
Bud Neck 0.0018 0.0005 0.0021 0.0038 0.0067 0.0017 0.0046 0.0003 0.002 0.0012 0.0066 0.0024 0.0005 0.0001 0.0051 0.0507 0.0006 0.0137
Bud Periphery 0.0004 0 0.0006 0.0012 0.0209 0.0012 0.0006 0.0021 0.0007 0.0004 0.0026 0.0002 0.0004 0 0.0003 0.0018 0.0001 0.0006
Bud Site 0.0054 0.0046 0.0038 0.0086 0.0056 0.0005 0.005 0.0082 0.0135 0.0029 0.0129 0.0002 0.0011 0.0009 0.0042 0.0046 0.0006 0.0002
Cell Periphery 0.0003 0.0001 0.0002 0.0003 0.0005 0 0.0002 0.0001 0.0001 0.0001 0.0005 0 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001
Cytoplasm 0.6328 0.7351 0.6397 0.5972 0.4768 0.7265 0.5259 0.7034 0.642 0.6657 0.4924 0.7263 0.7129 0.7996 0.7451 0.5287 0.6847 0.7336
Cytoplasmic Foci 0.0331 0.0165 0.0186 0.0197 0.0294 0.0182 0.0277 0.0094 0.0183 0.04 0.0405 0.0162 0.013 0.0046 0.007 0.0376 0.0305 0.0125
Eisosomes 0.0002 0 0.0003 0.0005 0.0002 0 0.0003 0 0.0002 0.0001 0.0004 0.0001 0.0004 0 0 0.0004 0.0001 0
Endoplasmic Reticulum 0.0064 0.0041 0.0042 0.0035 0.004 0.0035 0.0053 0.0033 0.0035 0.0046 0.0059 0.0033 0.0084 0.0045 0.0063 0.0039 0.0046 0.0066
Endosome 0.0331 0.0058 0.0127 0.0173 0.034 0.0072 0.0151 0.0037 0.0082 0.0167 0.026 0.0081 0.0129 0.0027 0.0037 0.0161 0.0234 0.0084
Golgi 0.0063 0.0006 0.0026 0.017 0.032 0.0013 0.0041 0.0003 0.0021 0.0031 0.0067 0.0015 0.0032 0.0015 0.0012 0.0129 0.0286 0.0007
Lipid Particles 0.0057 0.0001 0.0035 0.0062 0.0066 0.0022 0.0051 0.0001 0.0035 0.0025 0.0107 0.004 0.0088 0.0003 0.0058 0.0051 0.0214 0.0003
Mitochondria 0.0021 0.0004 0.0082 0.055 0.0607 0.001 0.0018 0.0009 0.0035 0.0014 0.0056 0.0021 0.0019 0.0009 0.0014 0.0202 0.0021 0.0004
None 0.2195 0.193 0.2328 0.1045 0.2394 0.1693 0.3236 0.2078 0.2375 0.1864 0.2267 0.1793 0.1579 0.1475 0.174 0.1758 0.0935 0.182
Nuclear Periphery 0.0048 0.0035 0.0037 0.0208 0.0021 0.0043 0.0076 0.0041 0.0032 0.0066 0.0193 0.0046 0.0085 0.0027 0.0032 0.0059 0.004 0.0039
Nucleolus 0.0009 0.0001 0.0009 0.0019 0.0005 0.0003 0.001 0.0004 0.0005 0.0003 0.0037 0.0002 0.0007 0.0001 0.0002 0.0005 0.0004 0.0001
Nucleus 0.0268 0.0239 0.0339 0.0444 0.0109 0.0467 0.0179 0.0405 0.0334 0.0276 0.0227 0.033 0.028 0.0222 0.0319 0.0245 0.0561 0.0267
Peroxisomes 0.0022 0.0066 0.0025 0.0099 0.0269 0.0034 0.0158 0.0009 0.008 0.0016 0.0093 0.0014 0.0065 0.0054 0.0044 0.009 0.0107 0.0002
Punctate Nuclear 0.0069 0.0028 0.0069 0.0357 0.0016 0.0086 0.0125 0.0086 0.007 0.0116 0.076 0.0073 0.0031 0.0011 0.002 0.0043 0.0238 0.0057
Vacuole 0.0038 0.0013 0.0024 0.0017 0.0076 0.0012 0.0023 0.0017 0.0014 0.0025 0.0033 0.001 0.0025 0.0012 0.0011 0.0038 0.0017 0.0014
Vacuole Periphery 0.0008 0.0002 0.0005 0.0013 0.0021 0.0002 0.0005 0.0003 0.0003 0.0007 0.0013 0.0003 0.0008 0.0002 0.0002 0.0008 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.7888 18.1074 17.6796 14.4626 13.8223 15.9825 15.4176 12.0317 15.2768 14.0563
Translational Efficiency 1.0862 1.1076 1.0367 1.5584 1.5163 1.3234 1.1736 1.6598 1.2637 1.4172

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II
Localization
Cell Percentages cytoplasm (74%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pih1

Pih1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pih1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available