Standard name
Human Ortholog
Description Essential nuclear envelope/ER integral membrane protein; interacts and functions with Apq12p and Brr6p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, mRNA nuclear export and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; identified as a dosage suppressor of a temperature sensitive mutation in the major karyopherin, CRM1; homologous to Brr6p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.05 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0.21 0.25 – 0.31 0.43 0.39 0.39 0.55 0.42 0.38 0.34 0.35 0.71 0.74 0.87 0.64 0.55 0.61 0.1 0.09 0.17 0.17 0.25 0.25
Endoplasmic Reticulum 0.28 0.41 – 0.31 0.1 0.16 0.08 0 0 0.12 0.06 0 0.16 0.12 0.09 0 0.08 0.08 0.43 0.48 0.49 0.45 0.38 0.26
Endosome 0.06 0.09 – 0 0 0 0 0 0 0 0 0 0.09 0.09 0 0.17 0.19 0.06 0.07 0.05 0 0.05 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.38 0.05 – 0.38 0.47 0.54 0.62 0.55 0.74 0.72 0.79 0.76 0 0 0 0.07 0 0.08 0.12 0.11 0.16 0.13 0.14 0.15
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.09 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.06 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0.07
Vac/Vac Membrane 0.14 0.33 – 0.12 0 0 0 0 0 0 0 0 0.05 0.06 0 0.11 0.24 0.1 0.08 0.07 0 0.05 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 5 – 0 0 1 0 0 0 0 0 0 3 0 0 2 1 5 6 12 1 5 5 9
Bud 3 2 – 0 1 2 4 4 6 1 1 2 5 2 0 1 0 0 1 5 0 3 2 1
Bud Neck 0 0 – 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 2 6 0 2 3 10
Bud Site 0 0 – 0 0 0 1 2 1 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 – 0 0 0 0 0 0 3 1 4 8 6 11 3 2 4 0 0 0 0 1 3
Cytoplasm 46 55 – 5 22 24 45 90 43 32 30 35 219 244 356 84 50 30 33 26 7 40 78 74
Endoplasmic Reticulum 60 89 – 5 5 10 9 3 0 10 5 3 50 39 36 5 7 4 144 131 22 105 117 76
Endosome 13 20 – 0 1 1 0 2 0 0 0 0 28 31 12 22 17 3 24 12 0 11 8 10
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 2 0 0 0 1
Mitochondria 82 11 – 6 24 33 71 91 75 61 69 76 1 3 1 9 2 4 39 30 7 29 43 44
Nucleus 3 2 – 0 1 0 0 0 1 0 1 1 1 0 2 2 1 1 2 1 0 0 0 0
Nuclear Periphery 19 1 – 0 0 1 1 0 1 2 2 4 0 0 1 0 1 0 37 16 0 7 9 3
Nucleolus 7 0 – 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 – 0 0 0 1 0 0 0 0 0 1 0 0 1 1 0 0 0 2 0 0 1
SpindlePole 1 0 – 0 1 0 1 2 1 0 1 0 4 2 1 8 2 4 6 7 0 5 6 20
Vac/Vac Membrane 29 72 – 2 1 1 4 6 1 0 0 2 14 19 15 15 22 5 27 20 1 12 24 24
Unique Cell Count 214 216 16 51 61 114 164 102 85 87 100 309 328 411 132 91 49 339 277 45 231 310 293
Labelled Cell Count 271 258 18 57 73 138 200 131 110 110 127 334 346 435 153 108 60 339 277 45 231 310 293


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.7 – 5.8 4.2 3.4 3.4 3.6 3.7 3.2 3.1 3.0 5.5 5.0 5.1 5.6 7.2 6.5 4.8 4.8 5.3
Std Deviation (1e-4) 0.6 0.9 – 2.5 1.4 0.9 1.4 1.2 1.6 1.3 0.5 0.9 1.1 0.9 1.1 1.5 1.6 1.0 1.3 1.0 1.1
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.4914 4.3939 4.6059 4.4397 4.0652 4.5914 3.2818 2.3259 3.0725 2.5114 3.1847 3.1227 5.7726 4.0281 3.6427 3.9323 3.7768 3.8061
Actin 0.0395 0.0015 0.008 0.0028 0.016 0.0183 0.07 0.0014 0.0127 0.0019 0.0136 0.0025 0.1766 0.004 0.0408 0.0042 0.0016 0.0008
Bud 0.0265 0.0001 0.0008 0.0019 0.0005 0.0022 0.0022 0.0005 0.0005 0 0.0002 0.0004 0.0025 0.0055 0.0001 0.0012 0 0.0004
Bud Neck 0.0269 0.0003 0.0047 0.0025 0.0825 0.0054 0.0146 0.0005 0.0003 0.0003 0.0008 0.0011 0.003 0.0016 0.0002 0.001 0.0003 0.0016
Bud Periphery 0.0059 0.0002 0.0006 0.0055 0.0008 0.002 0.0063 0.0006 0.0022 0.0001 0.0003 0.0005 0.0054 0.0033 0.0001 0.0004 0 0.0003
Bud Site 0.0047 0.0021 0.0168 0.0006 0.0044 0.0007 0.019 0.0132 0.0034 0.0001 0.0008 0.0052 0.0025 0.0095 0.0005 0.001 0.0001 0.0003
Cell Periphery 0.0022 0.001 0.0007 0.0008 0.0014 0.0006 0.0013 0.0009 0.0021 0.0004 0.0003 0.0004 0.0006 0.003 0.0004 0.0007 0.0001 0.0004
Cytoplasm 0.1222 0.3473 0.2424 0.3679 0.4359 0.2912 0.1852 0.1652 0.2567 0.2725 0.1864 0.2857 0.0501 0.3896 0.2327 0.3295 0.467 0.4358
Cytoplasmic Foci 0.0262 0.0152 0.0254 0.0135 0.0199 0.0213 0.024 0.0111 0.0185 0.0082 0.0543 0.0423 0.0497 0.0143 0.0101 0.0438 0.0113 0.0343
Eisosomes 0.0004 0.0002 0.0002 0.0002 0.0003 0.0001 0.0008 0.0002 0.0026 0.0001 0.0001 0.0002 0.0013 0.0005 0.0001 0.0003 0.0003 0.0001
Endoplasmic Reticulum 0.2571 0.2825 0.298 0.2094 0.1505 0.249 0.2903 0.1978 0.3382 0.4314 0.3249 0.2371 0.1239 0.1482 0.3372 0.2532 0.0875 0.2058
Endosome 0.0587 0.0477 0.0595 0.0492 0.0345 0.0503 0.0535 0.0249 0.0334 0.0235 0.137 0.0609 0.0882 0.0401 0.058 0.0464 0.0409 0.0261
Golgi 0.0206 0.0024 0.008 0.0011 0.0055 0.0023 0.0172 0.0014 0.0019 0.0014 0.0156 0.0028 0.0242 0.0014 0.0025 0.0017 0.0011 0.0009
Lipid Particles 0.0547 0.0248 0.0213 0.0069 0.0055 0.0358 0.0385 0.007 0.006 0.0087 0.0533 0.0196 0.0531 0.0057 0.0104 0.0251 0.0038 0.005
Mitochondria 0.0223 0.0017 0.0025 0.0033 0.0016 0.002 0.0044 0.0017 0.0076 0.0011 0.0033 0.0017 0.0132 0.0011 0.0013 0.0028 0.0008 0.0016
None 0.2073 0.1342 0.1728 0.203 0.1749 0.1486 0.1251 0.4879 0.1925 0.1839 0.0145 0.226 0.1549 0.2399 0.2052 0.1926 0.325 0.1504
Nuclear Periphery 0.0758 0.0964 0.0741 0.0593 0.032 0.0808 0.0855 0.06 0.0708 0.0453 0.072 0.0685 0.0935 0.0717 0.0758 0.0635 0.0407 0.0776
Nucleolus 0.0021 0.0006 0.0011 0.0021 0.0003 0.004 0.0047 0.0006 0.0003 0.0002 0.0005 0.0009 0.0022 0.0008 0.0002 0.0021 0.0006 0.0011
Nucleus 0.0115 0.0205 0.0144 0.0242 0.0132 0.0598 0.0231 0.0086 0.0063 0.0112 0.0106 0.0134 0.0349 0.0271 0.0119 0.0128 0.0088 0.0366
Peroxisomes 0.0128 0.0014 0.0019 0.0009 0.0014 0.0015 0.0063 0.0005 0.0004 0.0003 0.0894 0.002 0.0663 0.0003 0.001 0.0006 0.0003 0.0004
Punctate Nuclear 0.0075 0.0047 0.037 0.0222 0.0072 0.0132 0.0194 0.003 0.0346 0.0036 0.0097 0.0179 0.0255 0.0214 0.0023 0.0082 0.007 0.0066
Vacuole 0.0107 0.0127 0.0075 0.018 0.0104 0.0081 0.0065 0.0109 0.0072 0.0046 0.01 0.0086 0.0178 0.0089 0.0068 0.0067 0.002 0.0123
Vacuole Periphery 0.0047 0.0025 0.0025 0.0047 0.0013 0.0026 0.0023 0.0021 0.002 0.0012 0.0025 0.0023 0.0106 0.0021 0.0022 0.0023 0.0007 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.4678 8.3591 8.7873 10.6412 8.5877 8.5669 10.2127 8.5567 11.7867 12.4285
Translational Efficiency 1.0207 1.5464 1.4709 1.069 1.6411 1.3466 1.0511 1.3449 0.8842 0.9885

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1456 1475 1398 1148 696 1949 1193 1461 2152 3424 2591 2609

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 614.41 809.84 877.06 864.46 658.47 755.43 908.46 898.17 628.66 778.87 891.52 883.34
Standard Deviation 68.32 105.32 105.51 116.16 89.77 95.66 117.39 192.72 78.67 103.51 112.23 164.37
Intensity Change Log 2 — 0.398435 0.513474 0.492598 — 0.198180 0.464305 0.447871 — 0.298315 0.488248 0.469633

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000053 0.000351 0.000185 0.000866 0.000711 0.000448 0.000345 0.001398 0.000266 0.000406 0.000259 0.001164
Bud Neck 0.003192 0.004438 0.003618 0.008472 0.002024 0.004090 0.002897 0.009177 0.002814 0.004240 0.003286 0.008867
Bud Site 0.000678 0.002311 0.002631 0.009511 0.001169 0.002938 0.001668 0.016970 0.000837 0.002668 0.002188 0.013688
Cell Periphery 0.000142 0.000173 0.000126 0.000098 0.000200 0.000126 0.000139 0.000143 0.000161 0.000146 0.000132 0.000123
Cytoplasm 0.217829* 0.134414* 0.150760* 0.039137 0.352069* 0.127974* 0.160253* 0.046311 0.261245* 0.130748* 0.155131* 0.043155
Cytoplasmic Foci 0.149817 0.124827 0.016178 0.028510 0.170449 0.115720 0.027274 0.026416 0.156490 0.119643 0.021287 0.027337
Eisosomes 0.000138 0.000155 0.000041 0.000037 0.000128 0.000148 0.000058 0.000037 0.000135 0.000151 0.000049 0.000037
Endoplasmic Reticulum 0.007395 0.121506* 0.028804 0.038395 0.014671 0.107902* 0.033820 0.012710 0.009749 0.113762* 0.031113 0.024012
Endosome 0.008198 0.035801 0.012620 0.037709 0.018020 0.025509 0.016978 0.036437 0.011375 0.029942 0.014627 0.036997
Golgi 0.003134 0.017634 0.000208 0.001293 0.002441 0.014513 0.000892 0.006066 0.002910 0.015858 0.000523 0.003966
Lipid Particles 0.362546* 0.327183* 0.024764 0.065323 0.145388* 0.424230* 0.053963 0.065227 0.292313* 0.382424* 0.038208 0.065269
Mitochondria 0.005985 0.013019 0.002289 0.001653 0.003698 0.011701 0.001453 0.003433 0.005246 0.012269 0.001904 0.002650
Mitotic Spindle 0.005237 0.010353 0.024934 0.268652* 0.004181 0.006128 0.037109 0.372549* 0.004896 0.007948 0.030540 0.326833*
None 0.019471 0.003692 0.006484 0.000917 0.016639 0.002811 0.004535 0.002029 0.018555 0.003191 0.005587 0.001540
Nuclear Periphery 0.017615 0.025200 0.155975* 0.050087 0.018090 0.025553 0.090580 0.057314 0.017769 0.025401 0.125865* 0.054134
Nuclear Periphery Foci 0.071025 0.096006 0.405526* 0.384539* 0.081703 0.070029 0.441571* 0.275495* 0.074478 0.081219 0.422122* 0.323476*
Nucleolus 0.011724 0.002658 0.008839 0.002705 0.013144 0.002066 0.004972 0.006358 0.012183 0.002321 0.007059 0.004751
Nucleus 0.106307 0.039397 0.136185* 0.016674 0.135585* 0.031786 0.084742 0.020615 0.115776 0.035065 0.112498 0.018881
Peroxisomes 0.005719 0.005763 0.000794 0.002594 0.005069 0.005800 0.001441 0.004407 0.005509 0.005784 0.001092 0.003609
Vacuole 0.003525 0.031487 0.018567 0.041818 0.013934 0.018121 0.033870 0.032872 0.006892 0.023879 0.025613 0.036809
Vacuole Periphery 0.000267 0.003632 0.000472 0.001008 0.000685 0.002407 0.001439 0.004037 0.000402 0.002935 0.000917 0.002704

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.25 -10.74 -4.29 -2.84 -3.33 0.44 0.48 -1.08 -2.28 -2.33 -1.04 -0.59 -3.42 -3.27 -3.63
Bud Neck -1.60 -1.86 -6.65 -5.84 -6.00 -3.27 -2.72 -9.99 -6.95 -6.88 -2.46 -2.56 -10.58 -9.07 -9.22
Bud Site -3.45 -3.22 -7.18 -5.81 -4.95 -3.00 -3.37 -8.30 -7.09 -7.49 -4.67 -4.44 -10.92 -9.07 -8.82
Cell Periphery -3.85 0.24 2.68 5.34 2.27 5.69 3.10 1.41 -1.52 -0.27 1.79 1.77 1.30 0.48 0.41
Cytoplasm 8.65 5.19 21.08 13.28 14.61 17.79 12.52 23.08 10.17 12.38 17.75 11.12 29.43 16.24 18.65
Cytoplasmic Foci 6.01 26.73 24.77 20.39 -3.56 8.53 20.43 21.55 21.47 1.70 10.23 33.09 32.81 29.56 -1.33
Eisosomes -1.54 14.89 12.96 12.88 1.42 -1.32 9.05 13.27 12.00 5.61 -1.70 16.75 18.64 17.30 4.73
Endoplasmic Reticulum -21.49* -11.56 -10.38 14.92 -2.39 -21.47* -6.80 1.00 22.34 8.16 -31.24 -13.78 -8.62 26.13 3.72
Endosome -16.47 -5.99 -14.72 -0.57 -11.28 -3.85 0.25 -7.80 -5.09 -8.12 -16.95 -4.96 -17.47 -4.21 -13.79
Golgi -11.07 11.86 6.85 12.74 -5.90 -12.35 4.22 -1.88 7.43 -3.56 -16.36 10.42 -0.09 13.55 -4.38
Lipid Particles 2.38 38.46* 32.95* 32.79* -9.36 -27.35* 11.88 10.85 46.96* -1.03 -11.62 37.24 33.03 56.63* -6.79
Mitochondria -5.58 11.56 12.52 9.46 2.71 -8.95 5.67 0.91 9.52 -4.59 -9.47 13.55 8.90 13.45 -2.23
Mitotic Spindle -2.23 -7.71 -24.57 -24.04* -21.60 -1.10 -9.05 -36.30* -36.17* -29.98* -1.92 -11.74 -43.16 -42.80* -37.09*
None 11.10 8.45 13.72 3.86 6.48 9.42 7.35 9.76 1.07 4.04 14.66 11.23 16.29 3.44 7.31
Nuclear Periphery -4.36 -22.59 -9.93 -6.59 15.62 -3.60 -14.47 -10.19 -8.12 5.16 -5.84 -26.60 -14.39 -10.43 15.21
Nuclear Periphery Foci -4.16 -28.95* -25.18* -22.43* 2.60 2.13 -25.72* -14.22 -18.49 12.67 -1.27 -39.35 -28.08 -28.60* 11.26
Nucleolus 9.10 3.25 7.56 -2.96 6.33 7.45 3.36 2.81 -6.89 -0.79 11.73 4.69 6.41 -7.48 2.32
Nucleus 12.98 -6.67 16.04 4.68 19.82 14.81 4.00 14.76 1.18 13.49 20.09 -2.92 21.51 4.00 23.69
Peroxisomes 1.30 13.26 6.01 4.33 -3.12 -0.00 4.60 1.40 1.65 -2.94 1.05 11.51 4.56 3.71 -4.31
Vacuole -14.45 -12.03 -15.53 -8.48 -9.87 -2.93 -10.04 -11.95 -10.88 -1.46 -15.20 -15.54 -20.53 -13.70 -8.13
Vacuole Periphery -10.67 -3.61 -2.87 7.13 -1.88 -4.36 -0.97 -3.70 -0.80 -2.32 -9.59 -1.68 -4.59 1.89 -2.99
Standard name
Human Ortholog
Description Essential nuclear envelope/ER integral membrane protein; interacts and functions with Apq12p and Brr6p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, mRNA nuclear export and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; identified as a dosage suppressor of a temperature sensitive mutation in the major karyopherin, CRM1; homologous to Brr6p
Localization
Cell Percentages ER (14%)
Cell Cycle Regulation No
Subcompartmental Group ER-2

Brl1

Brl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Brl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available