Standard name
Human Ortholog
Description Inositol monophosphatase; involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.95 0.96 0.94 0.9 0.89 0.71 0.78 0.72 0.65 0.98 1.0 1.0 0.92 0.94 0.91 0.9 0.97 0.97 0.92 0.92 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.05 0.36 0.27 0.44 0.47 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0.07 0.08 0.05 0 0 0 0 0 0.09 0.08 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud 0 1 0 0 0 0 0 1 1 1 3 2 0 0 0 0 0 0 0 0 0 1 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 2 3 2 1 1 4 0 3 0 1 0 1 2 4 0 0 0 0 0 0
Cytoplasm 119 392 168 110 198 204 237 229 165 215 94 79 181 329 335 341 411 251 121 396 165 205 231 264
Endoplasmic Reticulum 1 0 0 1 1 3 1 1 1 0 0 6 3 1 0 14 19 7 1 0 0 2 1 0
Endosome 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 4 3 3 4 0 0 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Mitochondria 1 1 0 1 0 5 21 12 83 74 57 57 0 1 0 10 4 2 0 2 0 3 4 6
Nucleus 0 2 0 1 1 5 4 8 4 5 1 2 0 1 0 3 1 8 1 2 0 3 1 3
Nuclear Periphery 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0
Vac/Vac Membrane 0 2 4 4 10 3 7 18 19 15 4 1 7 1 6 35 33 34 0 2 0 3 1 2
Unique Cell Count 121 396 171 116 206 216 262 257 232 276 130 121 185 329 335 369 436 277 136 408 170 223 251 290
Labelled Cell Count 121 399 172 117 212 223 273 272 276 316 159 150 191 334 342 409 476 309 136 408 170 223 251 290


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.1 6.9 9.8 8.9 8.8 6.3 6.1 6.0 4.8 5.1 4.5 4.4 9.6 9.2 8.7 8.3 10.7 11.3 9.3 9.8 9.6
Std Deviation (1e-4) 1.7 1.1 1.7 1.8 1.6 0.9 1.7 1.3 1.1 1.3 0.7 0.6 1.8 1.6 1.6 1.5 2.1 2.3 2.1 2.4 2.2
Intensity Change (Log2) – – – -0.14 -0.16 -0.64 -0.7 -0.72 -1.05 -0.96 -1.12 -1.16 -0.04 -0.09 -0.18 -0.25 0.13 0.2 -0.08 -0.01 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -1.2 -1.9 -3.2 -3.6 -7.1 -6.0 -6.6 -7.7 -0.3 2.4 2.4 -2.7 -2.1 -3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2.8 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.8 0 0 7.4 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.3 0 0 2.1 2.5 1.6 0 0 0.8 0 0 3.0 2.4 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.9406 10.0686 8.5734 7.4433 7.2723 8.5251 5.8138 8.5793 7.2194 6.6756 6.0442 7.2008 3.9495 6.7107 5.6036 5.106 4.6682 5.8774
Actin 0.0233 0.0008 0.0055 0.0003 0.0236 0.0063 0.019 0.0016 0.0317 0.0015 0.0036 0.0009 0.0453 0.0002 0.0115 0.0295 0.044 0.0051
Bud 0.0003 0.0001 0.0002 0.0001 0.0052 0.0002 0.0004 0.0002 0.0031 0.0001 0.0002 0.0002 0.0005 0.0001 0.001 0.0002 0.0011 0.0003
Bud Neck 0.01 0.0002 0.0004 0.0004 0.0016 0.0018 0.0005 0.0063 0.0012 0.0007 0.0004 0.001 0.0023 0.0002 0.0053 0.001 0.0089 0.0019
Bud Periphery 0.0003 0 0.0001 0 0.0019 0.0002 0.0003 0.0002 0.0048 0 0.0002 0.0001 0.0006 0 0.0011 0.0001 0.0013 0.0002
Bud Site 0.0016 0.0006 0.0008 0.0002 0.0008 0.0002 0.0023 0.0109 0.0109 0.0002 0.0004 0.0001 0.0048 0.0002 0.0056 0.0014 0.0221 0.0002
Cell Periphery 0.0002 0.0001 0.0001 0 0.0002 0 0.0001 0.0001 0.0004 0 0.0001 0 0.0003 0 0.0002 0 0.0007 0
Cytoplasm 0.8469 0.9714 0.9237 0.9322 0.8207 0.9428 0.9178 0.9442 0.8533 0.9663 0.8318 0.9423 0.7355 0.9848 0.8981 0.8999 0.7343 0.9405
Cytoplasmic Foci 0.0163 0.0018 0.0078 0.002 0.0148 0.0056 0.006 0.0036 0.0123 0.0036 0.012 0.0034 0.0459 0.0013 0.0106 0.0216 0.0141 0.0112
Eisosomes 0.0002 0 0.0001 0 0.0009 0 0 0 0.0001 0 0 0 0.0004 0 0.0001 0.0001 0.0007 0
Endoplasmic Reticulum 0.0048 0.002 0.0024 0.0022 0.0048 0.0022 0.0052 0.0006 0.003 0.0019 0.0073 0.0017 0.0065 0.001 0.0046 0.0009 0.0074 0.0014
Endosome 0.0115 0.0006 0.0054 0.0007 0.0341 0.0017 0.0022 0.001 0.0191 0.0007 0.069 0.0014 0.0295 0.0004 0.009 0.0097 0.0482 0.004
Golgi 0.0069 0.0003 0.0021 0.0001 0.015 0.0003 0.001 0.0009 0.0099 0.0001 0.02 0.0001 0.011 0 0.007 0.0024 0.0257 0.0034
Lipid Particles 0.0044 0.0001 0.001 0 0.0148 0.0001 0.0004 0.0001 0.0008 0 0.0063 0 0.0291 0 0.0049 0.0022 0.0228 0.0007
Mitochondria 0.0015 0.0001 0.0024 0.0002 0.019 0.0003 0.0002 0.0002 0.0079 0.0002 0.0035 0.0002 0.0045 0.0001 0.0048 0.0005 0.0159 0.0016
None 0.0457 0.0103 0.0318 0.0214 0.011 0.0147 0.0374 0.0251 0.0263 0.0156 0.0174 0.0396 0.0133 0.0033 0.006 0.0028 0.0031 0.0032
Nuclear Periphery 0.0054 0.0009 0.0011 0.0023 0.0065 0.0016 0.0014 0.0004 0.0014 0.0009 0.014 0.0009 0.0143 0.0008 0.0029 0.0017 0.0143 0.001
Nucleolus 0.0004 0 0 0 0.0002 0 0 0 0.0001 0 0.0002 0 0.0006 0 0.0015 0.0001 0.0006 0.0001
Nucleus 0.0084 0.01 0.0089 0.0367 0.014 0.0197 0.0034 0.0031 0.0079 0.0069 0.0063 0.0069 0.0191 0.0069 0.0114 0.0161 0.0144 0.0129
Peroxisomes 0.0035 0 0.005 0 0.0062 0.0011 0.001 0.0009 0.0016 0.0001 0.0008 0.0001 0.0257 0.0001 0.0083 0.0032 0.0107 0.0104
Punctate Nuclear 0.0071 0.0004 0.0006 0.0008 0.0017 0.0007 0.0007 0.0003 0.0007 0.0009 0.003 0.0005 0.0081 0.0003 0.0047 0.006 0.0037 0.0011
Vacuole 0.0009 0.0004 0.0005 0.0003 0.0017 0.0007 0.0006 0.0002 0.003 0.0002 0.0019 0.0004 0.0016 0.0003 0.0011 0.0005 0.0031 0.0005
Vacuole Periphery 0.0003 0 0.0001 0.0001 0.0012 0.0001 0.0001 0 0.0005 0 0.0015 0.0001 0.0009 0 0.0003 0.0001 0.0029 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.698 7.7755 18.1335 19.1453 20.9436 9.9351 16.8467 21.6037 20.7734 15.5244
Translational Efficiency 2.8752 3.641 1.7596 1.9481 1.1313 2.8798 2.1833 1.6493 1.6139 2.1336

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1768 1757 223 1524 208 2111 383 1775 1976 3868 606 3299

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 978.98 1072.45 1206.19 1139.97 850.28 1109.58 1195.77 1135.72 965.43 1092.71 1199.60 1137.68
Standard Deviation 141.95 184.45 159.04 185.13 134.45 159.06 161.58 172.43 146.60 172.05 160.73 178.42
Intensity Change Log 2 — 0.131559 0.301106 0.219645 — 0.384004 0.491930 0.417597 — 0.254411 0.392952 0.315044

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000216 0.000401 0.001747 0.001403 0.000328 0.000202 0.000698 0.000371 0.000228 0.000292 0.001084 0.000848
Bud Neck 0.001121 0.001371 0.003563 0.004058 0.003816 0.002656 0.002569 0.005905 0.001405 0.002072 0.002935 0.005052
Bud Site 0.003624 0.004146 0.017844 0.025525 0.025942 0.007531 0.006870 0.029584 0.005973 0.005993 0.010908 0.027708
Cell Periphery 0.000083 0.000043 0.000295 0.000137 0.000364 0.000042 0.000285 0.000108 0.000112 0.000042 0.000288 0.000121
Cytoplasm 0.756262* 0.825138* 0.419074* 0.613027* 0.696920* 0.809573* 0.452776* 0.605864* 0.750016* 0.816643* 0.440374* 0.609173*
Cytoplasmic Foci 0.069531 0.025044 0.011231 0.009546 0.078717 0.040391 0.013253 0.011590 0.070498 0.033420 0.012509 0.010646
Eisosomes 0.000024 0.000006 0.000053 0.000018 0.000045 0.000011 0.000080 0.000015 0.000026 0.000009 0.000070 0.000016
Endoplasmic Reticulum 0.001489 0.000417 0.006522 0.001563 0.001674 0.000325 0.005667 0.000791 0.001508 0.000367 0.005982 0.001148
Endosome 0.003907 0.001582 0.004442 0.006282 0.008464 0.002578 0.006382 0.009951 0.004387 0.002125 0.005668 0.008256
Golgi 0.001036 0.000529 0.000256 0.005067 0.000343 0.000800 0.000190 0.006635 0.000963 0.000677 0.000214 0.005911
Lipid Particles 0.001677 0.000308 0.002277 0.001129 0.001566 0.000467 0.002817 0.000509 0.001666 0.000395 0.002618 0.000796
Mitochondria 0.001095 0.000309 0.000654 0.001618 0.000125 0.000838 0.000249 0.001940 0.000993 0.000597 0.000398 0.001792
Mitotic Spindle 0.002278 0.004053 0.003078 0.073124 0.000391 0.006673 0.002590 0.070271 0.002079 0.005483 0.002770 0.071589
None 0.002825 0.007404 0.003448 0.007448 0.002545 0.004127 0.010085 0.010892 0.002796 0.005615 0.007643 0.009301
Nuclear Periphery 0.000231 0.000125 0.001852 0.001155 0.000172 0.000171 0.001464 0.000859 0.000225 0.000150 0.001607 0.000996
Nuclear Periphery Foci 0.003854 0.001823 0.008436 0.002943 0.009095 0.000844 0.009939 0.002246 0.004406 0.001289 0.009386 0.002568
Nucleolus 0.000323 0.000174 0.000240 0.000238 0.000393 0.000290 0.000505 0.000994 0.000331 0.000237 0.000407 0.000645
Nucleus 0.089846 0.101539 0.355004* 0.143194* 0.052440 0.073581 0.193912* 0.147673* 0.085909 0.086281 0.253192* 0.145604*
Peroxisomes 0.000449 0.000391 0.000391 0.000309 0.000584 0.001163 0.000288 0.000441 0.000463 0.000813 0.000326 0.000380
Vacuole 0.059797 0.024963 0.159369 0.099902 0.115239 0.046830 0.289141* 0.088618 0.065633 0.036897 0.241387* 0.093831
Vacuole Periphery 0.000330 0.000235 0.000223 0.002312 0.000835 0.000910 0.000239 0.004743 0.000383 0.000603 0.000233 0.003620

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.54 -4.43 -2.49 -0.90 2.63 1.78 -7.04 1.60 -1.05 8.70 -0.24 -6.94 -2.22 -1.19 4.95
Bud Neck -1.44 -6.92 -12.33 -7.33 2.28 0.92 0.01 -1.30 -3.56 -2.51 -2.80 -10.05 -9.66 -5.99 -0.65
Bud Site -0.72 -2.40 -4.81 -4.36 0.71 3.61 3.44 2.55 -3.11 -2.74 0.06 -2.49 -4.61 -5.02 -0.77
Cell Periphery 8.39 -10.03 -2.80 -8.32 8.84 3.01 0.97 2.70 -5.95 10.43 5.90 -9.32 1.90 -10.17 13.33
Cytoplasm -8.28 26.25 31.03 39.67 -9.76 -5.66 16.16 9.52 35.26 -12.04 -9.24 36.63 35.79 52.54 -15.26
Cytoplasmic Foci 15.11 23.30 24.91 12.61 1.11 5.39 10.25 10.73 19.49 2.79 14.01 25.73 27.39 23.07 2.85
Eisosomes 13.78 -8.48 0.69 -14.53 8.77 6.18 -4.34 4.84 -8.45 12.29 13.57 -12.71 4.17 -16.28 14.84
Endoplasmic Reticulum 5.25 -8.76 1.14 -9.32 9.50 4.56 -7.77 2.77 -10.33 11.09 6.30 -11.91 2.58 -13.50 14.45
Endosome 4.04 -0.99 4.02 -1.01 4.37 4.69 2.65 4.09 -0.71 3.36 5.79 -1.04 4.19 -1.20 5.44
Golgi 1.51 2.69 0.29 -0.88 -1.52 -2.18 3.40 -2.34 -1.47 -2.59 1.14 2.85 -0.68 -1.70 -3.01
Lipid Particles 4.15 -3.46 0.31 -14.98 6.38 2.68 -2.86 1.73 -5.24 10.28 4.31 -3.90 1.62 -13.83 11.56
Mitochondria 1.43 0.75 0.55 -3.93 -0.96 -3.42 -5.18 -7.11 -0.69 -5.38 0.82 1.05 0.21 -2.26 -5.17
Mitotic Spindle -1.31 -1.57 -10.90 -10.45 -9.14 -5.07 -2.87 -11.65 -8.94 -9.18 -3.00 -2.04 -15.05 -13.69 -13.02
None -8.99 -0.82 -11.25 -3.04 -10.20 -2.18 -1.58 -8.36 -9.51 -2.69 -7.10 -1.56 -14.53 -9.25 -4.61
Nuclear Periphery 4.96 -19.15 -23.75 -24.89 3.49 -0.73 -15.65 -19.79 -20.25 4.61 3.18 -22.80 -30.73 -31.77 5.21
Nuclear Periphery Foci 3.32 -4.17 2.24 -1.98 6.39 5.36 0.28 4.94 -2.94 11.17 5.77 -5.45 4.53 -3.30 12.51
Nucleolus 1.90 1.05 -0.87 -8.25 -5.33 0.71 -0.48 -1.75 -1.97 -1.65 1.08 -0.14 -1.92 -2.67 -2.03
Nucleus -4.65 -16.66 -20.34 -16.67 10.07 -4.62 -15.64 -22.61 -25.62 2.25 -1.53 -20.38 -28.53 -30.26 8.13
Peroxisomes 0.94 2.04 2.34 1.05 0.37 -2.32 4.20 4.29 4.15 0.66 -2.00 3.34 3.51 4.22 0.56
Vacuole 7.95 -11.30 -23.80 -28.24 1.30 4.97 -12.71 -5.34 -21.47 10.86 6.36 -21.78 -27.88 -34.78 9.43
Vacuole Periphery 0.99 0.82 -0.73 -1.53 -1.43 -0.09 1.69 -0.73 -0.67 -2.60 -0.91 1.63 -2.04 -1.03 -2.92
Standard name
Human Ortholog
Description Inositol monophosphatase; involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Inm1

Inm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Inm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available