Standard name
Human Ortholog
Description Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.97 0.99 0.97 0.93 0.9 0.87 0.82 0.83 0.86 0.98 0.99 0.99 0.99 0.99 0.99 0.97 0.91 0.95 0.91 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.12 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 1 3 1 4 3 0 0 0 0 0 0 0 0 0 1 0 1
Bud 0 0 0 0 0 1 0 0 3 3 1 1 2 0 2 0 0 0 0 1 1 0 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 4 5 5 8 17 6 11 8 8 7 4 4 6 3 1 6 0 0 0 0 0 0
Cytoplasm 193 146 266 164 295 284 438 369 261 247 213 242 313 448 613 286 196 221 185 135 252 201 353 412
Endoplasmic Reticulum 0 0 4 2 1 4 21 24 11 6 5 14 1 2 7 11 5 4 0 0 0 8 9 12
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Mitochondria 1 1 0 1 0 1 2 2 9 10 15 2 1 1 0 1 0 0 0 0 2 0 2 0
Nucleus 0 0 1 2 3 3 6 7 3 6 6 2 3 1 4 2 1 0 0 4 2 0 2 6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 3 3 1 2 6 16 10 37 19 22 2 4 3 4 2 6 0 4 6 2 11 12
Unique Cell Count 194 147 273 169 299 292 469 411 299 303 258 283 320 454 622 290 198 224 191 149 267 221 387 461
Labelled Cell Count 194 149 278 177 305 303 493 425 312 318 271 294 327 461 636 308 205 237 191 149 267 221 387 461


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 33.8 27.4 25.1 20.9 21.6 18.7 17.9 17.0 14.6 13.3 13.1 13.9 26.6 27.4 27.2 42.5 39.4 41.4 25.7 26.9 29.1
Std Deviation (1e-4) 6.9 7.0 5.4 5.4 4.1 4.2 4.1 3.7 3.6 3.4 3.9 3.6 6.2 6.3 6.1 12.4 11.4 9.9 5.2 6.1 7.0
Intensity Change (Log2) – – – -0.27 -0.22 -0.42 -0.49 -0.56 -0.78 -0.92 -0.94 -0.85 0.08 0.13 0.11 0.76 0.65 0.72 0.04 0.1 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.1 1.7 0 1.7 1.0 1.3 0.9 0 0 0 0 0 0
Cytoplasm -0.2 1.1 -0.1 -2.4 -3.8 -4.5 -6.1 -5.8 -5.0 0.3 1.2 1.2 1.0 1.2 1.0
Endoplasmic Reticulum 0 0 0 2.2 2.8 1.7 0 0 2.3 0 0 0 1.7 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 4.0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.2 1.8 5.2 3.6 3.8 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.2851 22.6154 19.8754 16.1759 19.0469 20.6804 18.7289 26.4922 23.0856 22.3716 20.9291 23.141 11.0618 19.6598 16.4805 19.6283 20.0081 18.7302
Actin 0.0083 0.0003 0.0012 0.0631 0.0565 0.0005 0.0155 0.0001 0.0025 0.0011 0.004 0 0.0206 0.0098 0.0338 0.0001 0 0.0001
Bud 0.0004 0 0.0002 0.0002 0.0002 0.0002 0.0009 0.0013 0.0003 0.0011 0.0006 0 0.0026 0.0031 0.0035 0.0001 0 0
Bud Neck 0.0007 0.0002 0.0005 0.001 0.0009 0.0014 0.0017 0.0001 0.0002 0.0003 0.0007 0.0003 0.0103 0.0013 0.0005 0.0002 0.0002 0.0003
Bud Periphery 0.0005 0 0 0.0001 0.0004 0.0001 0.0006 0.0004 0.0002 0.0002 0.0006 0 0.0017 0.003 0.0036 0 0 0
Bud Site 0.0003 0.0001 0.0002 0.0004 0.0002 0.0002 0.006 0.0004 0.001 0.0001 0.0053 0 0.0456 0.0181 0.0048 0.0004 0 0
Cell Periphery 0.0001 0 0 0 0.0001 0 0.0003 0 0.0001 0 0.0001 0 0.0003 0.0006 0.0006 0 0 0
Cytoplasm 0.8807 0.9861 0.9404 0.9075 0.8666 0.9482 0.8958 0.9901 0.9292 0.9396 0.8475 0.9784 0.5839 0.8371 0.8049 0.8358 0.7467 0.9045
Cytoplasmic Foci 0.0125 0.0003 0.0052 0.0031 0.0011 0.0013 0.0152 0.0012 0.0078 0.0008 0.0136 0.0003 0.0165 0.009 0.0041 0.0482 0.0001 0.0006
Eisosomes 0.0001 0 0 0.0001 0.0003 0 0.0001 0 0 0 0.0001 0 0.0002 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0055 0.0005 0.0013 0.0008 0.0044 0.0019 0.0041 0.0002 0.0012 0.0003 0.0047 0.0004 0.0093 0.0003 0.0046 0.0004 0.0001 0.0003
Endosome 0.0111 0.0001 0.0021 0.0007 0.0117 0.0014 0.0126 0.0002 0.0199 0.0029 0.0329 0.0001 0.0533 0.0003 0.002 0.0965 0 0.0002
Golgi 0.0024 0 0.0003 0.0013 0.0053 0.0001 0.0084 0 0.0043 0.0045 0.0225 0 0.0142 0.0006 0.0043 0.0047 0 0
Lipid Particles 0.006 0 0.0008 0.001 0.0013 0 0.0087 0 0.0039 0.0001 0.0252 0 0.0062 0.0001 0.0003 0.001 0 0
Mitochondria 0.001 0 0.0002 0.0002 0.0019 0.0002 0.0016 0.0004 0.0065 0.0251 0.0195 0 0.0194 0.0001 0.0042 0.0004 0.0001 0
None 0.0099 0.0002 0.0036 0.0006 0.0022 0.0004 0.0012 0.0003 0.0006 0.0005 0.0003 0.0002 0.0234 0.0188 0.0165 0.0004 0 0.0003
Nuclear Periphery 0.0079 0.0016 0.0045 0.0016 0.0134 0.0051 0.0025 0.0004 0.0026 0.0014 0.0021 0.0023 0.0449 0.0003 0.0237 0.0007 0.0004 0.0017
Nucleolus 0.0002 0 0.0001 0 0.0001 0.0001 0.0005 0.0002 0.0003 0 0.0004 0 0.0017 0.0004 0.0003 0 0.001 0.0002
Nucleus 0.0409 0.0102 0.0328 0.0149 0.0303 0.0367 0.0144 0.0028 0.009 0.0207 0.0039 0.0175 0.1242 0.0951 0.0817 0.0054 0.2513 0.0913
Peroxisomes 0.0027 0 0.0002 0.0025 0.0004 0 0.0061 0.0001 0.0012 0.0001 0.0116 0 0.0028 0.0009 0.0026 0.0023 0 0
Punctate Nuclear 0.0041 0.0001 0.0052 0.0007 0.0012 0.0008 0.0014 0.0001 0.0009 0.0003 0.001 0.0001 0.0113 0.0003 0.0007 0.0007 0 0.0004
Vacuole 0.0039 0.0001 0.0007 0.0003 0.0005 0.0012 0.0022 0.0011 0.0061 0.0004 0.0021 0.0002 0.0036 0.0008 0.001 0.0018 0 0.0001
Vacuole Periphery 0.0009 0 0.0002 0.0001 0.001 0.0002 0.0004 0.0003 0.0021 0.0004 0.0013 0.0001 0.0043 0.0001 0.0022 0.0006 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 90.7701 95.9137 124.9782 135.4522 114.9052 63.4825 93.8749 100.7988 109.5372 103.4979
Translational Efficiency 1.0292 1.1013 1.0199 1.0949 1.0102 1.0058 0.9412 0.8464 0.9328 0.8549

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Aap1

Aap1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aap1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available