Standard name
Human Ortholog
Description Putative serine hydrolase; localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.96 0.92 0.89 0.81 0.76 0.74 0.75 0.69 0.77 1.0 0.99 1.0 0.96 0.93 0.94 0.93 0.91 0.98 0.95 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.11 0.07 0.1 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.09 0.16 0.21 0.18 0.16 0.23 0.13 0 0 0 0 0.06 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 0 2 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2 1 1 3 2 4 8 0 0 0 0 0 0 0 0 0 2 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 21 10 6 5 16 7 13 9 6 13 1 4 9 0 5 1 0 0 0 0 0 0
Cytoplasm 46 92 350 320 351 439 462 465 377 374 270 342 163 437 461 52 106 129 72 93 341 215 345 373
Endoplasmic Reticulum 0 0 0 1 5 9 10 5 6 13 4 6 0 4 1 1 2 3 0 1 2 0 2 1
Endosome 0 0 0 0 0 1 0 0 2 0 3 0 0 1 0 0 0 0 0 1 0 0 3 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 1
Mitochondria 0 1 0 1 0 5 14 5 54 35 38 42 0 1 0 0 5 4 0 0 0 0 0 1
Nucleus 1 1 2 2 1 6 5 9 6 11 7 12 2 2 1 1 0 0 0 2 0 2 1 0
Nuclear Periphery 0 0 0 0 0 1 0 1 4 2 0 2 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 5 12 26 47 89 130 92 78 90 56 1 0 0 1 7 12 2 1 1 1 3 4
Unique Cell Count 47 93 355 334 380 496 573 610 508 500 394 442 163 442 463 54 114 137 78 103 348 228 365 397
Labelled Cell Count 47 97 379 347 390 515 599 623 560 525 424 483 167 449 472 55 125 150 78 103 348 228 365 397


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.9 12.2 18.6 16.2 15.0 11.6 10.5 9.9 8.5 8.4 8.3 8.3 15.0 15.5 14.6 11.9 13.5 15.8 22.9 21.8 21.7
Std Deviation (1e-4) 4.4 4.7 3.8 4.0 3.0 2.5 2.5 2.0 2.0 2.0 1.9 2.0 3.7 3.5 3.2 5.7 7.1 9.6 6.0 6.6 6.9
Intensity Change (Log2) – – – -0.2 -0.31 -0.68 -0.82 -0.91 -1.13 -1.14 -1.16 -1.16 -0.31 -0.26 -0.35 -0.64 -0.46 -0.24 0.3 0.23 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.8 -3.1 -4.1 -2.4 -4.3 -2.5 -3.2 -3.2 -2.1 -2.8 -4.0 -3.0 0 -0.6 -2.5
Cytoplasm -2.2 -4.0 -5.6 -8.0 -9.2 -9.7 -9.5 -10.9 -8.8 1.5 0.3 1.5 -1.2 -3.2 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 3.1 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.0 0 6.3 5.1 6.0 6.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 1.9 0 2.3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.8 3.7 4.8 6.9 8.6 7.6 6.9 8.8 5.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.0768 12.626 11.2237 11.2102 11.5421 11.8306 12.2159 18.5654 15.7893 16.1537 15.1048 14.4707 8.7214 11.4703 10.4969 11.1495 10.0901 10.7608
Actin 0.0274 0.0019 0.0055 0.0001 0.0027 0.0003 0.0017 0.0003 0.0019 0.0077 0.0057 0.0019 0.0206 0.0002 0.0157 0.0002 0.0003 0.0297
Bud 0.0006 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0007 0.0001 0.0001 0.0007
Bud Neck 0.0014 0.0001 0.0003 0.0002 0.0003 0.0006 0.0003 0.0001 0.0004 0.0008 0.0004 0.0012 0.0019 0.0001 0.0005 0.0003 0.0003 0.0013
Bud Periphery 0.0009 0 0.0003 0 0 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0002 0.0002 0 0.0006 0 0 0.0006
Bud Site 0.0014 0.002 0.0007 0 0.0002 0 0.0004 0.0002 0.0003 0.0004 0.0004 0.0001 0.0005 0.0002 0.001 0 0.001 0.0005
Cell Periphery 0.0002 0.0001 0.0003 0 0 0 0.0002 0 0.0001 0.0001 0 0 0.0001 0 0.0001 0 0 0.0001
Cytoplasm 0.8017 0.9867 0.9365 0.9887 0.9265 0.9869 0.9014 0.9961 0.9628 0.9055 0.9061 0.9599 0.8919 0.9942 0.9589 0.9943 0.8927 0.8798
Cytoplasmic Foci 0.0193 0.0018 0.0041 0.0009 0.0075 0.0017 0.0028 0.0005 0.0026 0.0051 0.0452 0.0029 0.0217 0.0008 0.0057 0.001 0.0341 0.0096
Eisosomes 0.0003 0 0.0002 0 0 0 0 0 0 0.0001 0 0 0.0002 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0102 0.0005 0.0059 0.0048 0.0004 0.0018 0.0119 0.0004 0.012 0.0005 0.001 0.0021 0.0035 0.0003 0.0014 0.0005 0.0003 0.001
Endosome 0.0288 0.0005 0.0076 0.0003 0.008 0.002 0.008 0.0002 0.005 0.0139 0.0109 0.0148 0.0089 0.0002 0.0045 0.0002 0.0046 0.0107
Golgi 0.008 0.0002 0.0026 0 0.0091 0.0001 0.0003 0 0.0005 0.0313 0.0054 0.0074 0.0072 0 0.002 0 0.0038 0.0125
Lipid Particles 0.0093 0 0.0007 0 0.029 0 0.0006 0 0.0003 0.0048 0.0053 0.0003 0.0123 0 0.001 0 0.011 0.007
Mitochondria 0.005 0.0001 0.0184 0 0.0033 0.0002 0.0008 0.0001 0.0003 0.0248 0.004 0.0034 0.0024 0 0.0007 0 0.0012 0.0328
None 0.026 0.0037 0.0026 0.0009 0.0005 0.0006 0.0074 0.0004 0.0077 0.0003 0.0021 0.0008 0.0122 0.0019 0.0011 0.0005 0.0004 0.0007
Nuclear Periphery 0.0171 0.0004 0.0018 0.0008 0.0005 0.001 0.0435 0.0002 0.0022 0.0003 0.0005 0.0007 0.0043 0.0002 0.0007 0.0003 0.0007 0.0027
Nucleolus 0.0005 0 0.0001 0 0 0 0.0003 0 0 0 0 0 0.0002 0 0 0 0.0001 0.0001
Nucleus 0.0122 0.0014 0.0028 0.0027 0.004 0.0036 0.013 0.0011 0.0022 0.0016 0.0035 0.0032 0.0053 0.0013 0.0017 0.0021 0.0046 0.0029
Peroxisomes 0.0184 0 0.0004 0 0.0068 0 0.0001 0 0.0001 0.0015 0.007 0.0001 0.0029 0 0.0025 0 0.0437 0.0034
Punctate Nuclear 0.0045 0.0001 0.005 0.0001 0.0002 0.0001 0.0014 0 0.0006 0.0001 0.0014 0.0002 0.0025 0.0001 0.0004 0.0001 0.0007 0.0026
Vacuole 0.0042 0.0002 0.0024 0.0003 0.0003 0.0005 0.0031 0.0001 0.0006 0.0004 0.0002 0.0003 0.0007 0.0001 0.0005 0.0002 0.0002 0.0005
Vacuole Periphery 0.0026 0 0.0013 0.0001 0.0002 0.0001 0.0026 0 0.0002 0.0004 0.0001 0.0002 0.0005 0 0.0001 0 0.0001 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.99 63.3329 46.9957 40.4921 33.2246 61.7685 47.3456 50.1167 37.4407 36.7041
Translational Efficiency 2.1168 1.3078 1.2655 1.1841 1.4334 1.8784 1.5147 1.3072 1.5881 1.6879

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1381 1570 1743 1907 987 2172 172 1090 2368 3742 1915 2997

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 834.99 1045.08 1648.53 3027.65 978.29 1188.26 2020.16 2573.79 894.72 1128.19 1681.91 2862.58
Standard Deviation 145.02 227.83 334.80 919.34 171.78 209.01 618.76 647.81 171.92 228.32 384.32 859.12
Intensity Change Log 2 — 0.323783 0.981349 1.858368 — 0.280516 1.046135 1.395560 — 0.300601 1.016663 1.627196

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000236 0.000186 0.000172 0.000851 0.000248 0.000112 0.000637 0.000086 0.000241 0.000143 0.000214 0.000573
Bud Neck 0.002826 0.001004 0.000778 0.002121 0.000450 0.001262 0.006176 0.001144 0.001836 0.001154 0.001263 0.001766
Bud Site 0.010224 0.006299 0.004816 0.015178 0.004008 0.004198 0.014121 0.002972 0.007633 0.005079 0.005651 0.010739
Cell Periphery 0.000146 0.000070 0.000144 0.000756 0.000128 0.000047 0.000426 0.000227 0.000138 0.000057 0.000169 0.000564
Cytoplasm 0.707263* 0.863228* 0.895105* 0.855765* 0.866429* 0.831803* 0.623550* 0.870346* 0.773605* 0.844988* 0.870714* 0.861068*
Cytoplasmic Foci 0.186404* 0.047001 0.010228 0.007923 0.049597 0.048018 0.027896 0.006340 0.129382 0.047591 0.011815 0.007347
Eisosomes 0.000043 0.000009 0.000002 0.000003 0.000011 0.000010 0.000013 0.000001 0.000030 0.000010 0.000003 0.000003
Endoplasmic Reticulum 0.002364 0.000590 0.000057 0.000094 0.001255 0.000520 0.000712 0.000088 0.001902 0.000549 0.000116 0.000092
Endosome 0.008286 0.002211 0.001038 0.001572 0.002445 0.004006 0.004864 0.001817 0.005851 0.003253 0.001382 0.001661
Golgi 0.004119 0.001504 0.001365 0.002593 0.000770 0.001275 0.001758 0.002074 0.002723 0.001371 0.001400 0.002404
Lipid Particles 0.002275 0.000460 0.000677 0.001827 0.000717 0.000589 0.003210 0.001018 0.001626 0.000535 0.000904 0.001533
Mitochondria 0.000805 0.000766 0.000056 0.000393 0.000197 0.000861 0.000168 0.000069 0.000551 0.000821 0.000066 0.000275
Mitotic Spindle 0.000476 0.001087 0.003188 0.013958 0.001862 0.003437 0.006937 0.004983 0.001054 0.002451 0.003525 0.010694
None 0.003808 0.008134 0.002833 0.004367 0.006517 0.004047 0.006155 0.003732 0.004937 0.005762 0.003131 0.004136
Nuclear Periphery 0.000084 0.000066 0.000066 0.000121 0.000080 0.000119 0.001067 0.000224 0.000082 0.000097 0.000156 0.000158
Nuclear Periphery Foci 0.001364 0.002168 0.001744 0.001562 0.003273 0.000999 0.009822 0.003368 0.002160 0.001489 0.002469 0.002219
Nucleolus 0.000369 0.000345 0.000068 0.000236 0.000163 0.000348 0.000511 0.000144 0.000283 0.000347 0.000108 0.000203
Nucleus 0.030508 0.037856 0.060826 0.057142 0.045513 0.040005 0.104240 0.069549 0.036762 0.039103 0.064726 0.061654
Peroxisomes 0.000877 0.000445 0.000065 0.000215 0.000180 0.000566 0.000179 0.000024 0.000586 0.000515 0.000075 0.000145
Vacuole 0.035841 0.025399 0.016600 0.031843 0.015525 0.056760 0.169557 0.030888 0.027373 0.043602 0.030338 0.031496
Vacuole Periphery 0.001682 0.001172 0.000174 0.001479 0.000634 0.001017 0.017999 0.000903 0.001245 0.001082 0.001775 0.001269

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.76 -0.42 -2.00 -2.36 -1.95 3.52 -5.66 2.69 -1.95 7.28 2.61 -1.66 -1.64 -2.71 -1.06
Bud Neck 8.89 6.57 -0.27 -8.95 -6.71 -5.46 -9.04 -12.61 -6.00 6.52 4.57 -1.01 -5.40 -9.83 -3.92
Bud Site 4.32 4.78 -0.09 -3.25 -3.59 -0.24 -7.08 -2.05 -1.84 6.33 4.72 1.01 -0.99 -4.34 -1.73
Cell Periphery 9.76 -3.21 -6.73 -7.75 -6.06 8.23 -7.29 -6.53 -10.47 4.57 13.66 -6.37 -7.85 -9.59 -5.82
Cytoplasm -19.99 -19.90 -12.34 9.10 8.35 7.00 15.33 9.41 3.46 -11.96 -11.52 -7.63 -4.57 7.71 3.76
Cytoplasmic Foci 24.80 33.44 34.70 15.87 6.06 0.41 2.55 13.34 17.61 7.02 21.64 31.64 34.13 24.28 7.86
Eisosomes 13.76 17.27 16.85 6.18 -2.35 0.43 -3.00 8.33 12.32 9.85 12.90 17.47 18.03 12.32 2.82
Endoplasmic Reticulum 5.76 7.84 7.90 5.23 1.84 3.89 4.20 6.86 4.68 5.35 6.94 9.67 9.95 6.95 4.63
Endosome 9.26 11.17 12.01 5.27 -0.17 -2.55 -0.98 4.08 5.12 3.90 5.46 10.66 12.61 6.99 0.32
Golgi 5.12 6.36 6.60 0.51 -0.60 -1.60 -1.19 -0.25 0.75 0.34 4.97 4.28 5.38 0.70 -0.46
Lipid Particles 10.36 4.74 -4.12 -10.91 -7.54 1.24 -8.29 -6.54 -7.48 5.43 9.40 -0.24 -6.53 -12.67 -6.26
Mitochondria 0.15 8.84 6.45 1.08 -3.65 -1.80 1.74 3.23 2.23 3.29 -0.77 9.25 6.86 2.20 -3.37
Mitotic Spindle -1.92 -2.95 -5.47 -4.98 -3.65 -1.05 -2.79 -2.19 -1.53 1.41 -1.95 -2.68 -5.51 -4.29 -3.31
None -4.53 2.58 1.26 6.06 -2.35 2.34 2.70 3.60 1.85 0.77 -0.90 4.53 3.59 5.40 -2.21
Nuclear Periphery 1.18 -4.39 -7.63 -7.85 -5.05 -2.64 -6.65 -4.54 -3.38 5.00 -1.85 -7.57 -7.86 -6.53 -0.99
Nuclear Periphery Foci -4.05 -6.52 -5.56 -0.44 1.50 7.43 -6.32 -0.25 -5.20 5.70 3.49 -4.84 -2.61 -5.60 1.93
Nucleolus 0.28 3.97 2.23 1.67 -5.14 -1.73 -1.17 0.05 1.75 1.21 -0.74 4.24 2.12 2.21 -5.13
Nucleus -3.98 -17.62 -17.30 -13.89 0.66 1.56 -6.77 -9.13 -12.22 2.82 -2.18 -16.93 -18.55 -18.32 0.98
Peroxisomes 4.98 15.20 12.82 4.39 -2.28 -3.09 -0.94 4.20 4.26 6.82 0.95 14.69 12.96 5.40 -1.07
Vacuole 3.20 -2.15 -12.48 -14.62 -10.76 -16.65 -11.82 -16.06 -4.50 8.17 -10.63 -10.92 -20.18 -10.56 -5.51
Vacuole Periphery 1.14 8.84 4.26 0.98 -2.98 -1.14 -0.88 1.17 2.02 0.96 0.85 2.69 4.05 1.79 -0.43
Standard name
Human Ortholog
Description Putative serine hydrolase; localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Fsh1

Fsh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fsh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available