Standard name
Human Ortholog
Description Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; potential role in the secretory pathway; seamless-GFP and mCherry fusion proteins localize to the vacuole, while SWAT-GFP fusion localizes to both the endoplasmic reticulum and vacuole; suppresses toxicity of slow-folding human Z-type alpha1-antitrypsin variant associated with liver cirrhosis and emphysema
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.9 0.98 0.98 0.98 0.95 0.96 – 0.89 0.9 0.81 0.84 0.99 0.99 1.0 0.55 0.54 0.51 0.68 0.68 0.66 0.63 0.67 0.63
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.2 0 0 0 0.07 0 – 0.15 0.1 0.25 0.2 0 0 0 0 0 0 0.06 0.06 0.11 0.09 0.06 0.06
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.09 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.56 0.58 0.64 0.18 0.19 0.13 0.15 0.14 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 1 0 0 0 0 0 0 0 0 0 0 1 4 7 13
Bud 0 0 0 0 0 2 0 – 7 6 16 7 0 0 0 0 0 0 0 0 0 5 25 54
Bud Neck 2 0 0 2 8 4 23 – 10 13 12 7 0 0 0 0 0 0 1 0 0 8 6 15
Bud Site 0 0 0 0 0 1 0 – 0 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 1 0 0 – 0 4 0 1 1 2 2 0 0 0 0 0 0 4 4 12
Cytoplasm 214 37 58 251 337 241 524 – 259 370 396 398 524 939 985 223 146 120 147 55 61 327 510 711
Endoplasmic Reticulum 1 0 0 1 0 0 0 – 0 0 1 0 1 1 0 16 4 5 1 0 0 4 5 11
Endosome 0 0 0 0 0 1 5 – 0 2 1 3 1 3 0 6 11 5 4 0 0 13 17 23
Golgi 2 0 0 1 1 0 0 – 0 0 0 0 1 2 0 2 0 0 2 0 0 3 4 7
Mitochondria 4 8 0 0 2 18 9 – 45 42 123 93 0 0 1 6 1 3 13 5 10 47 42 68
Nucleus 0 0 0 1 2 1 4 – 7 4 0 3 0 1 0 47 24 14 0 0 0 1 0 2
Nuclear Periphery 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 0 – 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 3 1 1 10 – 6 7 9 21 1 0 0 0 0 0 0 1 0 9 13 31
Vac/Vac Membrane 5 0 0 1 5 1 15 – 1 6 3 8 1 0 5 226 156 151 40 15 12 78 107 139
Unique Cell Count 223 41 59 256 344 253 548 292 410 490 474 529 945 987 402 271 236 217 82 94 522 767 1124
Labelled Cell Count 229 45 59 260 357 271 590 336 457 564 544 531 948 993 527 342 298 217 82 94 522 767 1124


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.6 5.0 4.4 5.1 3.5 4.4 – 3.7 3.8 3.5 3.6 4.5 4.7 4.6 6.4 6.4 6.3 4.2 4.3 4.4
Std Deviation (1e-4) 0.8 0.5 2.0 1.2 1.7 1.2 1.5 – 1.5 1.1 1.1 0.8 1.2 1.5 1.3 1.4 1.2 1.2 1.6 2.0 1.9
Intensity Change (Log2) – – – -0.2 0.03 -0.5 -0.18 – -0.44 -0.4 -0.53 -0.49 -0.16 -0.1 -0.12 0.35 0.36 0.33 -0.25 -0.23 -0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 8.1 8.0 8.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -2.2372 -1.4881 -1.8083 -2.0839 -2.1875 -1.5962 -0.5293 -0.4057 -0.0284 -0.1377 -0.0796 0.1719 0.8914 2.1616 1.5446 1.7298 1.7904 1.8693
Actin 0.0182 0.0003 0.0015 0.0001 0.0008 0.0003 0.026 0.0004 0.0028 0.0002 0.0001 0.0039 0.0028 0 0.0006 0 0 0.0033
Bud 0.0004 0.0061 0.0054 0.0002 0.006 0.0023 0.0004 0.001 0.0006 0.0005 0.0006 0.0004 0.0003 0.0001 0.0003 0.0003 0.0001 0.0005
Bud Neck 0.001 0.0003 0.0006 0.0008 0.0003 0.0021 0.0019 0.0002 0.0007 0.0068 0.0004 0.001 0.002 0.0162 0.0003 0.0006 0.0004 0.0009
Bud Periphery 0.0007 0.0008 0.0024 0.0002 0.0033 0.0007 0.0006 0.0014 0.001 0.0006 0.0008 0.0008 0.0005 0.0002 0.0005 0.0004 0.0002 0.0014
Bud Site 0.001 0.016 0.0047 0.0005 0.0003 0.0004 0.001 0.0039 0.0015 0.0017 0.0007 0.0004 0.0022 0.0007 0.0009 0.0012 0.0001 0.0004
Cell Periphery 0.0002 0.0003 0.0004 0.0001 0.0002 0.0002 0.0003 0.0003 0.0003 0.0002 0.0002 0.0002 0.0004 0.0002 0.0004 0.0004 0.0001 0.0003
Cytoplasm 0.1442 0.1944 0.1768 0.1776 0.2103 0.248 0.0995 0.1258 0.1336 0.1301 0.0677 0.2094 0.0802 0.1977 0.1286 0.1186 0.1203 0.1877
Cytoplasmic Foci 0.0475 0.0877 0.0544 0.0709 0.0508 0.0787 0.0543 0.0235 0.0311 0.0492 0.0322 0.046 0.0301 0.0353 0.0232 0.0401 0.0228 0.029
Eisosomes 0.0005 0.0003 0.0002 0 0.0002 0.0001 0.0009 0.0005 0.0002 0 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0072 0.0032 0.0045 0.0022 0.004 0.0027 0.0037 0.0029 0.0031 0.0025 0.0013 0.0028 0.0025 0.002 0.0027 0.0019 0.0008 0.0021
Endosome 0.0763 0.0758 0.0845 0.1732 0.0791 0.0841 0.0555 0.0398 0.0856 0.0924 0.1512 0.0793 0.0463 0.0292 0.053 0.1067 0.0703 0.0717
Golgi 0.0023 0.0012 0.0013 0.0022 0.0007 0.0007 0.0048 0.0007 0.0026 0.0012 0.0024 0.0019 0.0027 0.0009 0.0023 0.0017 0.0004 0.0014
Lipid Particles 0.0107 0.0102 0.0153 0.0054 0.0049 0.0055 0.0141 0.0029 0.003 0.0037 0.002 0.007 0.0088 0.002 0.0097 0.0035 0.0015 0.0038
Mitochondria 0.002 0.0013 0.0038 0.0012 0.0017 0.0014 0.0032 0.0021 0.004 0.0014 0.0047 0.0031 0.0027 0.0006 0.0038 0.0081 0.0012 0.0015
None 0.6326 0.525 0.5663 0.4461 0.5973 0.4567 0.6736 0.7429 0.6744 0.5649 0.6277 0.5595 0.7845 0.65 0.7165 0.5806 0.7167 0.5829
Nuclear Periphery 0.0062 0.0029 0.0046 0.002 0.0027 0.003 0.0048 0.0018 0.002 0.0016 0.0012 0.0047 0.0034 0.001 0.0018 0.0017 0.001 0.0022
Nucleolus 0.001 0.0015 0.0021 0.0017 0.001 0.0017 0.0013 0.0007 0.0007 0.0013 0.0024 0.0019 0.0009 0.0008 0.0016 0.0011 0.0006 0.0015
Nucleus 0.0043 0.0089 0.0075 0.0049 0.0038 0.0119 0.0025 0.002 0.0019 0.0025 0.0033 0.0081 0.0016 0.0023 0.0023 0.0036 0.0013 0.0069
Peroxisomes 0.0014 0.0013 0.0019 0.0011 0.0007 0.001 0.0078 0.0003 0.0008 0.0081 0.0005 0.0016 0.0019 0.0022 0.0012 0.0008 0.0003 0.0021
Punctate Nuclear 0.0059 0.0079 0.0045 0.0041 0.0047 0.0049 0.0078 0.0009 0.0014 0.0026 0.0129 0.0053 0.0027 0.0004 0.0009 0.0005 0.0008 0.0029
Vacuole 0.0333 0.0511 0.0518 0.0997 0.0237 0.0871 0.0321 0.0426 0.0437 0.1198 0.0775 0.057 0.0216 0.05 0.0451 0.1167 0.053 0.0891
Vacuole Periphery 0.003 0.0036 0.0054 0.0056 0.0035 0.0065 0.0038 0.0032 0.0049 0.0087 0.0099 0.0055 0.0018 0.0081 0.004 0.0115 0.0079 0.0084

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.4943 25.5372 42.6369 45.8762 38.196 24.9824 55.2724 50.6479 57.424 41.1966
Translational Efficiency 1.6051 2.0823 1.432 1.7534 1.3877 2.8877 1.2254 1.4282 1.4247 1.3917

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; potential role in the secretory pathway; seamless-GFP and mCherry fusion proteins localize to the vacuole, while SWAT-GFP fusion localizes to both the endoplasmic reticulum and vacuole; suppresses toxicity of slow-folding human Z-type alpha1-antitrypsin variant associated with liver cirrhosis and emphysema
Localization
Cell Percentages cytoplasm (11%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cpr2

Cpr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cpr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available