Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.08 0.07 0.05 0 0.05 0.05 0 0 0 0.1 0.11 0.08 0.08 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.14 0 0 0 0.05 0 0 0.27 0.22 0.17 0.09 0 0 0 0.05 0.06 0.05 0 0 0.05 0 0 0
Nucleus 0.95 0.89 0.88 0.88 0.9 0.8 0.83 0.85 0.63 0.67 0.76 0.71 0.82 0.84 0.82 0.8 0.79 0.67 0.78 0.82 0.71 0.55 0.63 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 0.14 0.12 0.09 0 0.15 0.12 0.14 0.35 0.35 0.26 0.37 0.22 0.19 0.14 0.09 0.14 0.14 0.16 0.1 0.13 0.26 0.2 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.11 0 0 0 0.05 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 4 0 1 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0
Bud Site 1 0 0 1 0 5 0 2 4 5 1 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 2 1 3 8 12 12 7 5 5 6 4 3 13 21 13 13 18 0 0 2 1 4 3
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 4 4 6 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 2 2 0 0 0 0 2 0 2 7 4 3 1 4 0 1 4 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 36 0 1 0 8 5 6 26 20 28 13 1 0 2 8 10 8 0 1 3 1 0 4
Nucleus 148 238 36 68 95 134 183 154 60 62 121 99 84 113 155 132 134 113 133 231 54 31 105 98
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 1 0 1 3 1 0 0 0 0 0 0 0 2
Nucleolus 23 38 5 7 4 25 26 25 33 32 42 51 23 26 26 15 23 24 26 28 10 14 33 34
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 1 2 1 0 0 0 0 1 1 2 0 0 1 0 1 0 0 4 6
Vac/Vac Membrane 1 4 1 0 2 5 7 1 2 0 3 2 2 0 2 9 7 19 4 7 1 2 10 6
Unique Cell Count 155 266 41 77 105 167 220 181 95 92 160 139 103 135 189 166 169 168 171 281 77 57 168 167
Labelled Cell Count 174 320 43 82 109 190 237 198 130 124 205 175 116 155 214 189 195 192 171 281 77 57 168 167


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 7.0 9.5 9.1 9.6 7.6 8.0 7.7 6.3 6.2 6.3 7.1 8.7 8.6 8.1 8.2 10.0 10.2 7.6 10.0 9.4
Std Deviation (1e-4) 1.8 1.3 1.8 1.9 2.4 1.3 1.7 1.6 1.5 1.3 1.4 2.1 1.9 2.2 1.9 2.0 2.4 2.5 1.7 2.5 2.0
Intensity Change (Log2) – – – -0.07 0.0 -0.33 -0.26 -0.32 -0.61 -0.62 -0.6 -0.42 -0.13 -0.16 -0.24 -0.22 0.06 0.1 -0.32 0.07 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.7 3.2 2.9 0 0 0 0 0 0 0
Nucleus 0.1 0.5 -1.1 -0.7 -0.4 -2.9 -2.5 -1.7 -2.2 -0.9 -0.6 -0.9 -1.2 -1.2 -2.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.5 0 0.3 2.7 2.7 1.9 3.0 1.4 1.0 0.3 0 0.2 0.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.9554 8.1859 7.1417 6.1367 7.3277 7.1257 5.9674 7.2572 6.4696 5.429 5.9823 6.2715 3.3653 4.5245 3.8853 3.8475 3.7276 3.483
Actin 0.0256 0 0.0214 0.0551 0.0002 0.0003 0.0546 0 0.0002 0.002 0.0003 0.0016 0.0199 0 0.0076 0 0.0001 0.0004
Bud 0.0004 0.0001 0.0005 0.0001 0.0001 0.0001 0.0004 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0001
Bud Neck 0.0008 0.0003 0.0003 0.0002 0.0131 0.002 0.0023 0.0001 0.0002 0.0002 0.0003 0.0013 0.0005 0.0001 0.0005 0.0001 0.0014 0.0025
Bud Periphery 0.0006 0.0001 0.0013 0.0001 0.0002 0.0001 0.0006 0 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0 0.0006 0.0002
Bud Site 0.0018 0.0005 0.0018 0.0001 0.0008 0.0002 0.0016 0.0001 0.0012 0.0004 0.0002 0.0002 0.0004 0.0005 0.0004 0 0.0003 0.0003
Cell Periphery 0.0001 0 0.0002 0 0.0001 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0.0002 0.0001
Cytoplasm 0.0092 0.0001 0.0006 0.0004 0.0139 0.0004 0.0078 0.0001 0.1044 0.0164 0.1435 0.099 0.0027 0.0011 0.0002 0.0001 0.0001 0.0004
Cytoplasmic Foci 0.0343 0 0.0018 0.0021 0.001 0.0047 0.0045 0 0.0049 0.0026 0.0009 0.0019 0.0033 0 0.001 0 0 0.0003
Eisosomes 0.0004 0 0.0002 0.001 0 0 0.0009 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0024 0 0.0018 0.0005 0.0006 0.0001 0.0013 0 0.0018 0.0122 0.0045 0.002 0.0027 0 0.0001 0 0.0001 0.0004
Endosome 0.0191 0 0.0057 0.0017 0.001 0.0045 0.0041 0 0.0048 0.0075 0.0015 0.0043 0.0054 0 0.0024 0 0.0002 0.0006
Golgi 0.0088 0 0.0043 0.0024 0.0001 0.0009 0.0034 0 0.0002 0.0007 0.0001 0.0006 0.0019 0 0.0045 0 0 0.0001
Lipid Particles 0.0231 0 0.0075 0.0028 0.0003 0.0017 0.0032 0 0.0021 0.0019 0.0001 0.0004 0.0049 0 0.0006 0 0.0001 0.0005
Mitochondria 0.0169 0.0002 0.0271 0.0093 0.0003 0.0002 0.0017 0.0001 0.0008 0.0006 0.0003 0.0002 0.004 0.0001 0.0044 0.0001 0.0007 0.0003
None 0.0176 0 0.0004 0.0057 0.1075 0.0001 0.074 0 0.0029 0.0045 0.0068 0.0005 0.001 0 0.0001 0 0.0001 0.0001
Nuclear Periphery 0.0232 0.0007 0.0134 0.0013 0.0022 0.0004 0.0113 0.0008 0.0034 0.0047 0.0059 0.0035 0.0372 0.0006 0.001 0.0005 0.0045 0.002
Nucleolus 0.0376 0.0344 0.0227 0.0094 0.1164 0.0744 0.0383 0.0298 0.0341 0.0158 0.0279 0.0555 0.0221 0.0192 0.0164 0.0086 0.1493 0.0667
Nucleus 0.702 0.9626 0.8783 0.8988 0.7313 0.905 0.7327 0.9679 0.8285 0.9259 0.8013 0.8249 0.8774 0.9761 0.9583 0.99 0.838 0.9188
Peroxisomes 0.0407 0 0.0015 0.0077 0.0001 0.0013 0.0025 0 0.0002 0.0001 0 0.0001 0.0051 0 0.0007 0 0 0.0001
Punctate Nuclear 0.0333 0.001 0.0034 0.0009 0.0097 0.0033 0.0512 0.0009 0.0078 0.0024 0.0042 0.0036 0.0073 0.0018 0.0005 0.0004 0.0025 0.0058
Vacuole 0.001 0.0001 0.0029 0.0001 0.0007 0.0001 0.0025 0 0.0017 0.0012 0.0015 0.0002 0.0011 0.0001 0.0003 0 0.0009 0.0003
Vacuole Periphery 0.0011 0 0.0028 0.0001 0.0001 0 0.0008 0 0.0003 0.0004 0.0002 0.0001 0.0024 0 0.0003 0 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.396 22.2157 9.0667 14.8473 17.7215 12.4188 14.8812 22.7408 16.223 14.7977
Translational Efficiency 1.3172 0.9309 2.4514 1.5846 1.3894 1.9066 1.6713 1.0627 1.4994 1.9212

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1984 789 715 107 1740 1096 242 1578 3724 1885 957 1685

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 927.66 906.04 1166.55 1295.69 894.17 1094.03 1262.49 1226.93 912.01 1015.34 1190.81 1231.30
Standard Deviation 140.79 185.64 145.07 183.70 134.14 141.81 147.19 196.08 138.73 186.33 151.46 196.04
Intensity Change Log 2 — -0.034021 0.330580 0.482053 — 0.291031 0.497651 0.456432 — 0.134662 0.414998 0.469535

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000447 0.001003 0.001177 0.002335 0.000450 0.000460 0.002171 0.001565 0.000448 0.000687 0.001429 0.001613
Bud Neck 0.006805 0.010669 0.023627 0.013563 0.007646 0.012118 0.017722 0.045860 0.007198 0.011512 0.022134 0.043809
Bud Site 0.000630 0.001424 0.001709 0.002765 0.000923 0.000975 0.002536 0.008784 0.000767 0.001163 0.001918 0.008402
Cell Periphery 0.000050 0.000137 0.000037 0.000115 0.000044 0.000043 0.000067 0.000168 0.000047 0.000082 0.000045 0.000165
Cytoplasm 0.000564 0.004819 0.000179 0.004409 0.001263 0.001255 0.000376 0.008512 0.000891 0.002747 0.000229 0.008252
Cytoplasmic Foci 0.000134 0.000270 0.000052 0.000438 0.000141 0.000370 0.000089 0.001580 0.000137 0.000328 0.000061 0.001507
Eisosomes 0.000030 0.000036 0.000055 0.000050 0.000033 0.000035 0.000074 0.000033 0.000031 0.000036 0.000060 0.000034
Endoplasmic Reticulum 0.002430 0.002781 0.002022 0.004888 0.002022 0.002123 0.003600 0.002876 0.002239 0.002399 0.002421 0.003004
Endosome 0.000257 0.000199 0.000083 0.001439 0.000181 0.000325 0.000138 0.001626 0.000221 0.000272 0.000097 0.001614
Golgi 0.000157 0.000334 0.000168 0.001334 0.000141 0.000339 0.000250 0.004966 0.000150 0.000337 0.000189 0.004736
Lipid Particles 0.000087 0.000198 0.000085 0.000268 0.000226 0.000304 0.000145 0.000194 0.000152 0.000260 0.000100 0.000199
Mitochondria 0.000690 0.001221 0.001549 0.003810 0.000679 0.002073 0.002221 0.004178 0.000685 0.001716 0.001719 0.004154
Mitotic Spindle 0.000581 0.002945 0.001509 0.017066 0.001154 0.004241 0.001533 0.027039 0.000849 0.003699 0.001515 0.026406
None 0.001812 0.011819 0.001234 0.003504 0.001974 0.001820 0.001890 0.005634 0.001887 0.006005 0.001400 0.005498
Nuclear Periphery 0.000205 0.000129 0.000157 0.001153 0.000099 0.000239 0.000264 0.001716 0.000156 0.000193 0.000184 0.001681
Nuclear Periphery Foci 0.000305 0.000955 0.000121 0.000546 0.000277 0.000206 0.000161 0.000611 0.000292 0.000520 0.000131 0.000607
Nucleolus 0.079999 0.097162 0.085260 0.016941 0.103533 0.093152 0.089184 0.026303 0.090995 0.094830 0.086252 0.025709
Nucleus 0.903889* 0.861569* 0.878710* 0.862803* 0.878107* 0.878547* 0.872771* 0.853672* 0.891843* 0.871440* 0.877208* 0.854252*
Peroxisomes 0.000514 0.001130 0.001471 0.000907 0.000691 0.000681 0.002948 0.000435 0.000596 0.000869 0.001844 0.000465
Vacuole 0.000183 0.000856 0.000589 0.021197 0.000242 0.000259 0.001613 0.003066 0.000211 0.000509 0.000848 0.004217
Vacuole Periphery 0.000231 0.000345 0.000207 0.040471 0.000175 0.000434 0.000247 0.001181 0.000205 0.000397 0.000217 0.003676

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.74 -9.02 -9.84 -7.89 -6.41 -0.46 -14.46 -13.17 -13.01 6.02 -7.17 -14.30 -15.23 -11.97 -0.33
Bud Neck -4.57 -14.47 -7.42 -5.91 -0.47 -5.15 -8.31 -24.51 -21.05 -10.15 -7.02 -16.63 -25.42 -22.29 -12.57
Bud Site -3.78 -7.13 -7.13 -6.32 -5.49 -0.17 -7.10 -7.26 -7.30 -3.02 -2.32 -9.43 -8.17 -7.65 -5.46
Cell Periphery -4.66 0.54 -7.63 -2.69 -8.19 0.17 -4.03 -7.90 -8.59 -5.17 -3.40 -1.40 -9.59 -5.84 -9.55
Cytoplasm -3.50 2.24 -3.23 -0.68 -3.42 -0.26 1.25 -5.29 -4.89 -6.77 -2.95 2.09 -6.51 -3.71 -7.39
Cytoplasmic Foci -1.01 1.00 -1.45 -0.31 -3.23 -1.01 0.88 -2.22 -1.00 -2.52 -1.31 1.40 -2.25 -1.26 -2.70
Eisosomes -2.79 -12.26 -7.59 -6.63 -3.72 -1.33 -11.68 -3.15 -1.63 10.64 -3.07 -16.32 -6.92 -3.47 10.95
Endoplasmic Reticulum -1.81 0.43 -7.44 -6.79 -7.03 -0.62 -6.91 -6.12 -5.78 2.79 -1.32 -2.12 -7.25 -6.11 -2.68
Endosome 0.48 1.23 -2.65 -3.46 -3.96 -1.13 -0.77 -3.71 -2.60 -3.60 -0.42 1.26 -3.49 -3.32 -4.38
Golgi -1.84 -0.71 -3.01 -2.08 -2.90 -1.54 -6.37 -2.43 -2.14 -2.11 -2.10 -2.71 -2.45 -2.19 -2.31
Lipid Particles -0.80 -0.14 -4.72 -2.50 -4.76 -0.20 0.13 0.11 0.34 -0.08 -0.55 0.28 -0.75 0.15 -2.25
Mitochondria -3.51 -8.55 -9.56 -7.43 -5.73 -1.77 -11.88 -15.77 -3.07 -5.68 -2.24 -12.57 -17.18 -5.54 -9.81
Mitotic Spindle -1.45 -1.88 -3.14 -2.96 -2.95 -1.99 -1.80 -8.56 -6.94 -4.31 -2.54 -2.51 -9.25 -7.90 -7.54
None -4.96 1.84 -4.28 3.26 -4.80 0.17 -0.99 -6.19 -6.00 -4.24 -4.68 0.63 -7.63 0.94 -8.11
Nuclear Periphery 1.32 -0.10 -6.46 -6.81 -6.54 -2.32 -7.90 -11.64 -10.32 -9.07 -0.75 -3.31 -11.86 -11.62 -11.21
Nuclear Periphery Foci -2.41 1.60 -1.55 0.43 -1.99 -0.33 0.76 -2.80 -1.55 -3.42 -2.15 1.82 -2.86 0.22 -4.03
Nucleolus -3.59 -3.07 8.24 9.62 9.01 1.87 0.65 18.08 13.44 6.38 -1.47 -1.34 19.60 17.18 12.48
Nucleus 7.11 7.24 3.66 1.61 1.57 0.34 3.29 7.30 6.24 0.88 5.46 6.96 10.59 5.87 3.07
Peroxisomes -3.43 -5.15 -3.01 -0.51 1.59 0.09 -6.55 1.92 0.72 7.70 -1.28 -7.66 1.07 1.98 9.27
Vacuole -2.16 -3.43 -3.21 -3.11 -3.11 -0.27 -6.37 -7.83 -7.82 -1.98 -2.20 -6.06 -7.54 -6.91 -5.57
Vacuole Periphery -1.20 0.11 -1.02 -1.00 -1.02 -2.31 -5.51 -2.65 -1.02 -1.85 -2.43 -1.10 -1.90 -1.44 -1.81
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Med6

Med6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Med6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available