Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress; GIC1 has a paralog, GIC2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 6 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 1 | 0 | 17 | 1 | 8 | 6 | 7 | 6 | 17 | 16 | 6 |
Bud | 5 | 7 | 4 | 5 | 1 | 2 | 5 | 5 | 2 | 10 | 3 | 9 | 8 | 1 | 0 | 2 | 2 | 2 | 4 | 21 | 9 | 20 | 11 | 13 |
Bud Neck | 0 | 0 | 2 | 1 | 6 | 4 | 1 | 6 | 1 | 6 | 1 | 7 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 12 | 6 | 33 | 13 | 4 |
Bud Site | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 2 | 6 | 0 | 0 | 6 | 3 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 4 | 4 | 8 | 5 | 8 | 2 | 2 | 0 | 0 | 0 | 3 | 6 | 5 |
Cytoplasm | 42 | 107 | 79 | 102 | 238 | 214 | 238 | 304 | 162 | 271 | 201 | 252 | 271 | 392 | 476 | 170 | 118 | 135 | 56 | 77 | 74 | 170 | 350 | 364 |
Endoplasmic Reticulum | 3 | 12 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 6 | 5 | 0 | 8 | 3 | 6 | 1 | 0 | 1 | 2 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 4 | 13 | 6 | 1 | 3 | 2 | 8 | 5 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 2 |
Mitochondria | 22 | 3 | 1 | 17 | 4 | 35 | 83 | 51 | 98 | 93 | 112 | 76 | 0 | 1 | 0 | 3 | 3 | 1 | 8 | 6 | 6 | 8 | 31 | 20 |
Nucleus | 0 | 2 | 1 | 2 | 1 | 0 | 4 | 6 | 4 | 0 | 4 | 2 | 13 | 5 | 1 | 5 | 8 | 1 | 3 | 13 | 5 | 2 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 3 | 2 | 4 | 2 | 1 | 7 | 0 | 4 | 3 | 6 | 14 | 1 | 3 | 11 | 9 | 6 | 2 | 3 | 4 | 7 | 11 | 15 |
Vac/Vac Membrane | 1 | 11 | 4 | 5 | 7 | 0 | 1 | 0 | 1 | 1 | 2 | 2 | 20 | 7 | 18 | 48 | 42 | 35 | 9 | 26 | 13 | 5 | 4 | 5 |
Unique Cell Count | 62 | 138 | 94 | 121 | 253 | 241 | 299 | 347 | 240 | 336 | 265 | 306 | 334 | 411 | 493 | 245 | 178 | 185 | 99 | 175 | 137 | 287 | 467 | 461 |
Labelled Cell Count | 74 | 151 | 102 | 137 | 261 | 257 | 333 | 384 | 275 | 387 | 327 | 361 | 365 | 423 | 506 | 286 | 205 | 211 | 99 | 175 | 137 | 287 | 467 | 461 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 4.9 | 5.0 | 4.4 | 5.0 | 3.7 | 3.5 | 4.0 | 3.1 | 3.3 | 3.1 | 3.3 | 5.5 | 5.6 | 5.6 | 7.1 | 7.3 | 7.4 | 4.9 | 5.0 | 5.2 |
Std Deviation (1e-4) | 0.4 | 0.9 | 1.2 | 1.2 | 1.9 | 1.0 | 1.5 | 1.6 | 0.9 | 1.0 | 1.2 | 1.1 | 1.1 | 1.2 | 1.8 | 1.3 | 1.7 | 1.7 | 1.1 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.16 | 0.01 | -0.41 | -0.49 | -0.3 | -0.69 | -0.59 | -0.69 | -0.59 | 0.14 | 0.18 | 0.17 | 0.52 | 0.56 | 0.57 | -0.01 | 0.0 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | 3.0 | 1.2 | -1.0 | 0.9 | -3.0 | -0.7 | -1.6 | -0.4 | -0.6 | 4.0 | 4.9 | -2.7 | -3.1 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 0 | 0 | 3.5 | 4.0 | 3.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.2569 | -0.3166 | -0.0469 | 0.0342 | -0.2796 | -0.2317 | -0.1666 | 0.2937 | 0.8978 | 1.1451 | 0.9731 | 0.5523 | -0.4108 | 0.3548 | 0.4559 | 0.9008 | 0.9163 | 0.3019 |
Actin | 0.0549 | 0.0042 | 0.0168 | 0.0009 | 0.0048 | 0.003 | 0.0432 | 0.0033 | 0.0158 | 0.0029 | 0.0112 | 0.0054 | 0.0336 | 0.0034 | 0.0136 | 0.008 | 0.0832 | 0.009 |
Bud | 0.0045 | 0.002 | 0.0291 | 0.0012 | 0.0077 | 0.0057 | 0.0095 | 0.0068 | 0.0381 | 0.0011 | 0.0038 | 0.0008 | 0.0229 | 0.0045 | 0.0436 | 0.0059 | 0.0055 | 0.0018 |
Bud Neck | 0.0243 | 0.0025 | 0.0076 | 0.0009 | 0.0014 | 0.0522 | 0.0251 | 0.0053 | 0.0023 | 0.0017 | 0.0166 | 0.1065 | 0.018 | 0.0042 | 0.0041 | 0.0273 | 0.0045 | 0.0993 |
Bud Periphery | 0.0176 | 0.0031 | 0.0396 | 0.001 | 0.0171 | 0.0048 | 0.0221 | 0.0054 | 0.0538 | 0.001 | 0.0071 | 0.0015 | 0.0384 | 0.0101 | 0.071 | 0.0078 | 0.0135 | 0.0021 |
Bud Site | 0.0316 | 0.0405 | 0.0197 | 0.0018 | 0.002 | 0.0061 | 0.0505 | 0.0555 | 0.0234 | 0.0014 | 0.0065 | 0.0006 | 0.0232 | 0.0339 | 0.0205 | 0.0055 | 0.0162 | 0.0013 |
Cell Periphery | 0.001 | 0.0027 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.001 | 0.0009 | 0.001 | 0.0001 | 0.0004 | 0.0001 | 0.0009 | 0.0012 | 0.0006 | 0.0004 | 0.0005 | 0.0002 |
Cytoplasm | 0.1057 | 0.2937 | 0.2734 | 0.371 | 0.4377 | 0.2643 | 0.2422 | 0.3444 | 0.3851 | 0.5728 | 0.4051 | 0.3819 | 0.3049 | 0.4889 | 0.3826 | 0.429 | 0.4827 | 0.503 |
Cytoplasmic Foci | 0.0911 | 0.0326 | 0.0265 | 0.0106 | 0.0155 | 0.0299 | 0.0713 | 0.0261 | 0.0228 | 0.0105 | 0.0511 | 0.0467 | 0.0779 | 0.007 | 0.0419 | 0.0181 | 0.0414 | 0.0594 |
Eisosomes | 0.0009 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0003 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0121 | 0.0026 | 0.0072 | 0.005 | 0.0095 | 0.0021 | 0.0046 | 0.0034 | 0.0057 | 0.004 | 0.0058 | 0.0018 | 0.0082 | 0.0048 | 0.0077 | 0.015 | 0.0089 | 0.0037 |
Endosome | 0.0566 | 0.0029 | 0.0167 | 0.0048 | 0.0065 | 0.005 | 0.0223 | 0.0066 | 0.0119 | 0.003 | 0.0315 | 0.0046 | 0.0196 | 0.0027 | 0.0144 | 0.0411 | 0.0302 | 0.0111 |
Golgi | 0.0154 | 0.0007 | 0.0055 | 0.0003 | 0.0022 | 0.0013 | 0.0052 | 0.0009 | 0.0023 | 0.0003 | 0.0102 | 0.0008 | 0.0043 | 0.0003 | 0.0042 | 0.0024 | 0.0087 | 0.0022 |
Lipid Particles | 0.0279 | 0.0005 | 0.0047 | 0.0002 | 0.0131 | 0.0091 | 0.0076 | 0.0005 | 0.0022 | 0.0003 | 0.0432 | 0.0026 | 0.0092 | 0.0002 | 0.0052 | 0.0016 | 0.0051 | 0.0026 |
Mitochondria | 0.0222 | 0.0015 | 0.0125 | 0.0014 | 0.002 | 0.0086 | 0.0051 | 0.0016 | 0.0103 | 0.0009 | 0.0141 | 0.0023 | 0.0128 | 0.0006 | 0.0072 | 0.0068 | 0.0034 | 0.0072 |
None | 0.4305 | 0.5536 | 0.4654 | 0.4112 | 0.3662 | 0.5563 | 0.3926 | 0.4589 | 0.2657 | 0.1713 | 0.2581 | 0.356 | 0.345 | 0.2749 | 0.1408 | 0.1326 | 0.1236 | 0.2004 |
Nuclear Periphery | 0.0347 | 0.0032 | 0.0068 | 0.0114 | 0.0082 | 0.0074 | 0.0175 | 0.0028 | 0.0087 | 0.0042 | 0.0198 | 0.0032 | 0.0095 | 0.0046 | 0.0118 | 0.026 | 0.0265 | 0.0064 |
Nucleolus | 0.0016 | 0.0009 | 0.0013 | 0.0015 | 0.001 | 0.0017 | 0.0023 | 0.0014 | 0.0022 | 0.001 | 0.0109 | 0.0012 | 0.0028 | 0.0008 | 0.0025 | 0.0017 | 0.0016 | 0.0008 |
Nucleus | 0.0112 | 0.0385 | 0.0364 | 0.0873 | 0.0782 | 0.0242 | 0.0326 | 0.0585 | 0.1133 | 0.2085 | 0.0461 | 0.051 | 0.0307 | 0.1487 | 0.1748 | 0.2441 | 0.0935 | 0.0687 |
Peroxisomes | 0.0321 | 0.0043 | 0.01 | 0.0007 | 0.0091 | 0.0026 | 0.0153 | 0.0027 | 0.0102 | 0.0011 | 0.0194 | 0.0039 | 0.0192 | 0.0003 | 0.0186 | 0.0012 | 0.0201 | 0.0054 |
Punctate Nuclear | 0.0156 | 0.008 | 0.0153 | 0.0861 | 0.015 | 0.0119 | 0.0242 | 0.0119 | 0.0203 | 0.0124 | 0.0335 | 0.028 | 0.0106 | 0.0065 | 0.0287 | 0.0129 | 0.0261 | 0.0128 |
Vacuole | 0.0045 | 0.0015 | 0.0036 | 0.0021 | 0.002 | 0.0021 | 0.0038 | 0.0026 | 0.0038 | 0.0012 | 0.0038 | 0.0009 | 0.0061 | 0.002 | 0.0053 | 0.0092 | 0.0034 | 0.0017 |
Vacuole Periphery | 0.0041 | 0.0002 | 0.0011 | 0.0005 | 0.0004 | 0.0012 | 0.0009 | 0.0003 | 0.0008 | 0.0002 | 0.0013 | 0.0002 | 0.0016 | 0.0002 | 0.0011 | 0.003 | 0.0015 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.0115 | 15.6568 | 3.8727 | 7.6053 | 16.6526 | 23.3395 | 12.6644 | 9.2599 | 9.6672 | 18.2506 |
Translational Efficiency | 1.6999 | 1.3228 | 1.7327 | 1.1908 | 1.29 | 1.0218 | 1.0764 | 1.1452 | 0.9618 | 1.1644 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1135 | 534 | 249 | 109 | 1821 | 2101 | 1793 | 1661 | 2956 | 2635 | 2042 | 1770 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 692.04 | 783.79 | 918.08 | 925.63 | 669.35 | 723.22 | 914.02 | 865.83 | 678.06 | 735.49 | 914.52 | 869.51 |
Standard Deviation | 77.74 | 89.95 | 110.45 | 123.05 | 77.88 | 89.50 | 104.28 | 125.15 | 78.61 | 92.84 | 105.06 | 125.84 |
Intensity Change Log 2 | — | 0.179612 | 0.407764 | 0.419580 | — | 0.111674 | 0.449465 | 0.371323 | — | 0.146609 | 0.428418 | 0.396055 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000852 | 0.001206 | 0.001660 | 0.003455 | 0.000233 | 0.000571 | 0.000488 | 0.002234 | 0.000471 | 0.000699 | 0.000631 | 0.002309 |
Bud Neck | 0.027037 | 0.037616 | 0.020482 | 0.038196 | 0.025754 | 0.054508 | 0.012653 | 0.024781 | 0.026247 | 0.051085 | 0.013608 | 0.025607 |
Bud Site | 0.020907 | 0.035484 | 0.037835 | 0.073455 | 0.013561 | 0.035778 | 0.015746 | 0.058053 | 0.016381 | 0.035718 | 0.018440 | 0.059001 |
Cell Periphery | 0.000566 | 0.000623 | 0.000547 | 0.000802 | 0.000315 | 0.000377 | 0.000403 | 0.000482 | 0.000411 | 0.000427 | 0.000421 | 0.000502 |
Cytoplasm | 0.424691* | 0.359279* | 0.192735* | 0.210394* | 0.455488* | 0.413370* | 0.316097* | 0.442173* | 0.443663* | 0.402408* | 0.301054* | 0.427899* |
Cytoplasmic Foci | 0.190700* | 0.228254* | 0.017338 | 0.012037 | 0.150512 | 0.120399 | 0.011102 | 0.019675 | 0.165942 | 0.142256 | 0.011862 | 0.019205 |
Eisosomes | 0.000265 | 0.000291 | 0.000143 | 0.000169 | 0.000157 | 0.000152 | 0.000063 | 0.000105 | 0.000199 | 0.000180 | 0.000073 | 0.000109 |
Endoplasmic Reticulum | 0.001066 | 0.001910 | 0.016288 | 0.022218 | 0.001276 | 0.001059 | 0.003693 | 0.002324 | 0.001195 | 0.001231 | 0.005228 | 0.003549 |
Endosome | 0.006471 | 0.014526 | 0.004058 | 0.010834 | 0.003351 | 0.007537 | 0.001382 | 0.007449 | 0.004549 | 0.008954 | 0.001708 | 0.007658 |
Golgi | 0.001602 | 0.006163 | 0.000214 | 0.006765 | 0.001154 | 0.003604 | 0.000066 | 0.002943 | 0.001326 | 0.004123 | 0.000084 | 0.003178 |
Lipid Particles | 0.008805 | 0.006059 | 0.003253 | 0.002499 | 0.005322 | 0.003779 | 0.001191 | 0.002165 | 0.006659 | 0.004241 | 0.001442 | 0.002186 |
Mitochondria | 0.006976 | 0.011692 | 0.000702 | 0.000832 | 0.004413 | 0.008257 | 0.000672 | 0.003412 | 0.005397 | 0.008953 | 0.000676 | 0.003253 |
Mitotic Spindle | 0.000336 | 0.003235 | 0.001134 | 0.003115 | 0.001618 | 0.004915 | 0.000862 | 0.018176 | 0.001126 | 0.004574 | 0.000895 | 0.017249 |
None | 0.006004 | 0.005626 | 0.003237 | 0.004543 | 0.005774 | 0.005362 | 0.006259 | 0.009827 | 0.005862 | 0.005415 | 0.005891 | 0.009502 |
Nuclear Periphery | 0.000504 | 0.000388 | 0.001707 | 0.001257 | 0.000561 | 0.000436 | 0.001151 | 0.000901 | 0.000539 | 0.000427 | 0.001219 | 0.000923 |
Nuclear Periphery Foci | 0.000402 | 0.000655 | 0.002503 | 0.002545 | 0.000365 | 0.000293 | 0.000752 | 0.000880 | 0.000379 | 0.000367 | 0.000965 | 0.000983 |
Nucleolus | 0.001804 | 0.002844 | 0.000933 | 0.000374 | 0.000859 | 0.001812 | 0.000477 | 0.000777 | 0.001222 | 0.002021 | 0.000533 | 0.000752 |
Nucleus | 0.256381* | 0.206354* | 0.613022* | 0.455510* | 0.298141* | 0.275958* | 0.587590* | 0.288760* | 0.282107* | 0.261852* | 0.590691* | 0.299029* |
Peroxisomes | 0.015416 | 0.029948 | 0.004058 | 0.001060 | 0.005092 | 0.013420 | 0.000576 | 0.002101 | 0.009056 | 0.016769 | 0.001001 | 0.002037 |
Vacuole | 0.028640 | 0.046400 | 0.077907 | 0.149037 | 0.025650 | 0.047434 | 0.038646 | 0.109273 | 0.026798 | 0.047225 | 0.043433 | 0.111722 |
Vacuole Periphery | 0.000575 | 0.001448 | 0.000243 | 0.000905 | 0.000405 | 0.000979 | 0.000131 | 0.003510 | 0.000470 | 0.001074 | 0.000144 | 0.003349 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.87 | -2.39 | -2.15 | -1.90 | -0.76 | -5.74 | -4.32 | -2.73 | -1.99 | -1.86 | -1.45 | -1.94 | -2.41 | -2.00 | -1.73 |
Bud Neck | -2.10 | 1.39 | 0.99 | 2.20 | 0.06 | -7.75 | 4.84 | 1.60 | 9.72 | -3.75 | -8.22 | 5.78 | 2.14 | 9.90 | -3.45 |
Bud Site | -2.93 | -1.78 | -1.89 | -0.84 | -0.89 | -8.42 | -0.98 | -8.49 | -1.49 | -7.34 | -8.21 | -0.75 | -8.02 | -1.73 | -6.96 |
Cell Periphery | -0.24 | -0.42 | -0.41 | -0.05 | -0.07 | -1.08 | -1.40 | -1.36 | 0.20 | 0.32 | -0.17 | 0.10 | 0.68 | 0.75 | 0.68 |
Cytoplasm | 4.62 | 17.21 | 12.26 | 8.24 | -0.43 | 5.03 | 19.93 | 9.49 | 4.86 | -8.95 | 5.81 | 23.57 | 10.59 | 5.29 | -9.75 |
Cytoplasmic Foci | -3.12 | 27.18 | 27.39 | 21.54 | 0.62 | 5.45 | 32.38 | 30.88 | 29.05 | -4.15 | 4.88 | 43.29 | 41.52 | 35.08 | -3.74 |
Eisosomes | -0.47 | 2.82 | 2.10 | 1.89 | -0.23 | 0.47 | 9.21 | 4.81 | 3.12 | -5.69 | 1.15 | 9.77 | 7.00 | 4.43 | -4.35 |
Endoplasmic Reticulum | -3.70 | -10.47 | -5.39 | -5.09 | 0.64 | 1.14 | -12.90 | -8.75 | -12.07 | 6.38 | -0.51 | -16.38 | -10.43 | -10.59 | 6.73 |
Endosome | -4.44 | 3.00 | 0.50 | 2.96 | -0.36 | -7.02 | 8.13 | -1.70 | 3.73 | -4.65 | -7.05 | 9.82 | 0.12 | 5.40 | -4.29 |
Golgi | -3.89 | 7.75 | -0.29 | 1.61 | -0.97 | -3.99 | 7.00 | -0.58 | 2.92 | -3.08 | -5.15 | 10.51 | -0.34 | 3.82 | -3.21 |
Lipid Particles | 1.82 | 4.53 | 5.18 | 2.66 | 1.48 | 2.88 | 10.09 | 5.97 | 3.43 | -1.14 | 4.03 | 10.36 | 7.54 | 4.25 | -0.69 |
Mitochondria | -1.55 | 3.93 | 4.01 | 4.49 | 1.30 | -2.90 | 3.62 | 1.95 | 6.32 | -2.72 | -2.92 | 5.33 | 3.29 | 7.20 | -2.69 |
Mitotic Spindle | -3.24 | -2.46 | -1.39 | -1.07 | -1.27 | -2.73 | 0.34 | -6.46 | -4.54 | -7.00 | -3.76 | -0.47 | -7.04 | -4.91 | -7.06 |
None | 0.33 | 4.77 | 3.56 | 1.92 | -0.04 | 0.49 | 0.45 | -0.79 | -1.15 | -1.08 | 0.70 | 1.14 | -0.51 | -0.94 | -1.25 |
Nuclear Periphery | 1.19 | -11.27 | -6.85 | -7.58 | 0.73 | 0.62 | -4.75 | -5.28 | -14.13 | -1.63 | 0.85 | -8.07 | -8.11 | -15.87 | -0.95 |
Nuclear Periphery Foci | -3.34 | -6.27 | -6.43 | -5.86 | -1.21 | 0.49 | -6.80 | -4.25 | -4.55 | 1.42 | -0.45 | -9.15 | -6.03 | -5.67 | 1.82 |
Nucleolus | -0.92 | 1.95 | 2.92 | 2.20 | 3.92 | -4.29 | 4.86 | 1.13 | 4.52 | -2.00 | -2.55 | 3.45 | 2.43 | 4.44 | -1.55 |
Nucleus | 3.79 | -18.92 | -5.37 | -6.72 | 5.59 | 2.55 | -34.52 | -4.51 | -6.92 | 28.09 | 2.76 | -42.01 | -7.67 | -9.66 | 28.91 |
Peroxisomes | -3.91 | 5.91 | 8.53 | 8.80 | 1.35 | -6.52 | 7.26 | 5.63 | 10.47 | -3.03 | -5.70 | 10.64 | 9.85 | 13.45 | -1.77 |
Vacuole | -5.45 | -9.16 | -9.76 | -8.87 | -6.15 | -9.52 | -10.73 | -27.41 | -22.34 | -20.17 | -10.77 | -13.31 | -28.94 | -24.01 | -20.36 |
Vacuole Periphery | -3.34 | 2.85 | 0.74 | 3.17 | -0.59 | -5.89 | 7.52 | -2.79 | -1.61 | -3.37 | -6.02 | 6.39 | -2.64 | -1.34 | -3.37 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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