Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.1 0 0 0 0 0 0 0 0 0.11 0.11 0.16 0.07 0.05 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0.06 0 0.07 0.05 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0 0.06 0 0.08 0.16 0.17 0.13 0.19 0.21 0.18 0.2 0 0 0 0.08 0.08 0.11 0 0 0 0 0 0
Nucleus 0.29 0.4 0.12 0.07 0.08 0.08 0.07 0.1 0.09 0.11 0.09 0.13 0.06 0 0.05 0.21 0.14 0.16 0.11 0.19 0.05 0.09 0.09 0.07
Nuclear Periphery 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.71 0.67 0.83 0.84 0.81 0.8 0.79 0.76 0.74 0.73 0.79 0.73 0.83 0.84 0.76 0.7 0.76 0.72 0.75 0.73 0.68 0.7 0.64 0.59
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.21 0.2 0.3 0.27 0.27 0.31 0.25 0.24 0.27 0.26 0.29 0.26 0 0 0 0 0 0.12 0.06 0.09 0.09
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0.05 0 0.06 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 4 1 9 7 9 10 10 11 0 0 0 0 0 0 0 0 4 2 9 12
Bud Neck 0 0 2 1 3 1 4 4 1 10 0 7 9 15 11 0 0 0 0 0 1 1 3 2
Bud Site 0 0 2 1 5 4 13 19 13 22 21 30 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 2 1 8 15 11 10 13 14 12 5 6 4 46 57 86 10 11 15 0 0 0 1 3 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 0 0 1 0 0 1
Endosome 0 1 6 4 21 13 18 35 16 13 21 22 9 14 17 2 2 1 1 0 12 7 16 32
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2
Mitochondria 32 5 18 5 24 36 70 62 68 100 67 95 3 2 2 11 17 18 1 0 3 7 13 16
Nucleus 50 52 37 10 26 18 29 50 30 51 34 65 24 18 24 29 29 26 20 27 16 34 55 39
Nuclear Periphery 8 0 8 2 0 2 2 4 8 5 1 9 0 1 1 1 1 1 6 1 12 6 10 12
Nucleolus 122 86 250 126 254 181 319 372 259 354 296 354 338 452 402 99 160 120 130 107 223 260 371 334
Peroxisomes 1 0 3 4 5 5 9 12 10 5 9 6 6 4 7 0 0 0 0 0 0 0 0 4
SpindlePole 2 0 21 32 62 68 111 135 110 122 89 132 105 155 137 2 8 0 6 1 40 23 51 49
Vac/Vac Membrane 2 2 6 0 4 1 2 6 5 8 5 4 1 5 8 7 11 9 7 7 7 20 41 42
Unique Cell Count 173 129 303 150 312 225 404 492 352 483 375 484 408 538 529 141 210 166 175 147 329 373 584 570
Labelled Cell Count 219 149 361 200 420 340 599 720 541 706 559 740 542 723 696 163 240 192 175 147 329 373 584 570


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 17.2 14.3 12.1 9.7 8.1 7.6 7.2 6.6 6.6 6.3 6.3 14.4 13.6 12.5 20.4 20.8 23.0 16.4 15.7 16.7
Std Deviation (1e-4) 3.3 4.6 4.2 4.6 3.1 1.9 2.1 2.0 1.8 1.7 2.0 1.6 5.5 4.9 5.1 8.6 8.0 10.8 5.1 4.4 4.2
Intensity Change (Log2) – – – -0.24 -0.56 -0.82 -0.92 -0.99 -1.11 -1.11 -1.19 -1.19 0.01 -0.07 -0.2 0.51 0.54 0.68 0.2 0.13 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 2.1 0 0 0 0
Bud Site 0 0 0 2.3 2.7 2.6 3.1 3.5 3.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.4 0.6 1.1 0.4 0.2 0.6 -1.7 -0.9 0 4.3 4.1 6.0 2.2 1.5 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.9 2.3 1.8 3.2 1.8 0.6 2.4 1.9 0.2 0.6 1.0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.2 0.9 3.8 4.5 3.0 5.1 5.6 4.7 5.3 -4.1 -5.1 -5.0 0.7 1.0 1.9
Nucleus -1.8 -1.6 -1.6 -2.3 -0.9 -1.6 -0.7 -1.3 0.5 -3.0 -5.0 -4.1 2.3 0.5 1.0
Nuclear Periphery 0 0 0 0 0 -0.3 -1.7 0 -0.7 0 0 0 0 0 0
Nucleolus 0.4 -0.4 -0.6 -1.2 -2.3 -2.7 -3.0 -1.2 -3.0 0.1 0.6 -2.2 -2.9 -1.8 -2.6
Peroxisomes 0 0 0 1.3 1.5 1.7 0 1.4 0 0 0 0 0 0 0
SpindlePole 4.5 4.7 7.1 6.9 7.1 7.8 6.5 5.9 7.0 6.5 7.5 6.7 -2.4 -1.5 -3.5
Vacuole 0 0 0 0 0 -0.6 -0.3 0 0 0 0 -0.5 0 2.0 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.0476 13.6165 13.3824 12.5164 10.7879 12.6022 12.3322 14.5813 12.9655 13.018 12.513 13.1336 12.6248 13.5803 12.2836 12.1364 11.6154 11.9812
Actin 0.0023 0 0 0 0.029 0 0.0019 0.0001 0.0021 0 0 0.0019 0.0094 0 0.0006 0 0.0001 0
Bud 0.0001 0 0 0.0001 0.0003 0 0.0002 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0 0 0
Bud Neck 0.0003 0.0001 0.0001 0.0002 0.0024 0.0001 0.0002 0.0002 0.0009 0.0001 0.0001 0.0002 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001
Bud Periphery 0.0002 0 0 0.0001 0.0006 0.0001 0.0005 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0001 0 0 0.0001 0.0001
Bud Site 0.0005 0.0001 0 0.0002 0.0015 0.0001 0.0005 0.0004 0.003 0 0.0002 0.0001 0.0003 0.0001 0.0001 0 0 0
Cell Periphery 0.0003 0.0002 0.0001 0.0002 0.0005 0.0002 0.0006 0.0001 0.0001 0.0001 0.0006 0.0003 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0008 0 0 0 0.0129 0 0.0008 0 0.0001 0 0.0002 0.0002 0.0011 0 0.0002 0 0 0
Cytoplasmic Foci 0.0016 0.0001 0.0003 0.0001 0.0042 0.0008 0.0079 0.0058 0.0027 0.0004 0.0025 0.0012 0.0113 0.0003 0.0013 0.0002 0.0001 0.0003
Eisosomes 0.0001 0 0 0 0.0005 0 0 0 0 0 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0004 0 0 0 0.0025 0 0.0019 0 0 0 0.0001 0.0002 0.0006 0 0 0 0 0
Endosome 0.0028 0.0001 0 0 0.0025 0.0001 0.0029 0.0046 0.0002 0 0.0009 0.0009 0.0039 0 0.0002 0 0.0001 0
Golgi 0.0005 0 0 0 0.0015 0.0001 0.0004 0.0007 0.0002 0.0001 0.0001 0.0003 0.0013 0 0.0001 0 0.0001 0
Lipid Particles 0.0027 0.0006 0.0007 0.0003 0.021 0.0052 0.0061 0.0048 0.0026 0.0015 0.0055 0.005 0.0085 0.0012 0.0043 0.0004 0.0024 0.0034
Mitochondria 0.0021 0.0003 0.0001 0.0001 0.017 0.002 0.0017 0.0002 0.0009 0 0.0038 0.0041 0.0013 0.0019 0.0002 0.0001 0.0009 0.002
None 0.0029 0 0 0 0.0225 0 0.0008 0 0.0002 0 0.0002 0.0003 0.0019 0 0.0036 0 0.0001 0
Nuclear Periphery 0.0134 0.001 0.0007 0.0002 0.0886 0.0031 0.0098 0.004 0.0001 0.0011 0.0004 0.0128 0.0045 0.0034 0.0003 0.0001 0.0513 0.0004
Nucleolus 0.8379 0.8499 0.8994 0.8874 0.5409 0.8636 0.8376 0.8359 0.8773 0.8413 0.8717 0.868 0.8893 0.8894 0.9145 0.8795 0.838 0.9019
Nucleus 0.1241 0.1396 0.0959 0.1092 0.1987 0.117 0.1157 0.1369 0.1061 0.1433 0.0932 0.0917 0.0549 0.0937 0.0688 0.1185 0.0868 0.0843
Peroxisomes 0.0004 0 0 0 0.0012 0.0001 0.0005 0.0022 0.0003 0 0.0003 0.0003 0.0019 0.0001 0.0004 0 0.0001 0
Punctate Nuclear 0.0036 0.0011 0.0023 0.0009 0.0419 0.0062 0.0066 0.0033 0.002 0.0118 0.0119 0.0094 0.0076 0.0012 0.0047 0.0006 0.016 0.0061
Vacuole 0.0009 0.0048 0.0002 0.0006 0.0014 0.0003 0.0017 0.0004 0.0005 0.0001 0.005 0.0007 0.0006 0.0006 0.0002 0.0002 0.0003 0.0002
Vacuole Periphery 0.0019 0.002 0.0002 0.0003 0.0084 0.0008 0.0017 0.0003 0.0006 0.0001 0.0029 0.0024 0.0009 0.0073 0.0002 0.0002 0.0034 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 52.2537 47.798 48.9031 41.2319 56.7203 34.0069 31.4867 28.1705 29.6675 45.4397
Translational Efficiency 1.7222 1.5112 1.3107 1.3626 1.1841 1.5199 1.5085 1.0556 1.0749 1.2394

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2021 442 1928 802 1631 2126 158 1452 3652 2568 2086 2254

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 973.28 1295.03 1525.61 1739.78 1149.92 1301.65 1557.17 1735.33 1052.17 1300.51 1528.00 1736.91
Standard Deviation 234.71 302.47 277.67 370.06 236.26 253.60 320.39 487.38 251.25 262.67 281.25 449.17
Intensity Change Log 2 — 0.412059 0.648459 0.837978 — 0.178808 0.437393 0.593677 — 0.290428 0.537992 0.710818

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000748 0.000518 0.000577 0.000993 0.000374 0.000574 0.000478 0.000958 0.000581 0.000565 0.000569 0.000971
Bud Neck 0.001531 0.001721 0.002204 0.007761 0.001423 0.003617 0.001859 0.008744 0.001483 0.003291 0.002178 0.008394
Bud Site 0.001639 0.000275 0.000186 0.001373 0.000277 0.000804 0.000200 0.001801 0.001031 0.000713 0.000187 0.001648
Cell Periphery 0.000187 0.000022 0.000015 0.000073 0.000027 0.000066 0.000011 0.000172 0.000116 0.000059 0.000015 0.000137
Cytoplasm 0.001602 0.000138 0.000018 0.000253 0.000085 0.000366 0.000013 0.001717 0.000925 0.000326 0.000018 0.001197
Cytoplasmic Foci 0.000474 0.001396 0.000295 0.007621 0.002103 0.000894 0.000725 0.007419 0.001201 0.000981 0.000327 0.007491
Eisosomes 0.000095 0.000102 0.000107 0.000184 0.000159 0.000120 0.000087 0.000188 0.000124 0.000117 0.000106 0.000187
Endoplasmic Reticulum 0.002362 0.000607 0.000232 0.000436 0.000567 0.000913 0.000249 0.000474 0.001561 0.000860 0.000233 0.000461
Endosome 0.000399 0.000081 0.000049 0.002073 0.000211 0.000430 0.000030 0.001493 0.000315 0.000370 0.000047 0.001699
Golgi 0.000278 0.000162 0.000105 0.001003 0.000363 0.000195 0.000160 0.001664 0.000316 0.000189 0.000110 0.001429
Lipid Particles 0.000119 0.000100 0.000019 0.000289 0.000235 0.000162 0.000026 0.000478 0.000171 0.000151 0.000020 0.000411
Mitochondria 0.001808 0.001223 0.001756 0.006434 0.001733 0.001679 0.001582 0.005910 0.001775 0.001600 0.001743 0.006097
Mitotic Spindle 0.000457 0.000388 0.002515 0.020864 0.003594 0.004923 0.002842 0.011067 0.001858 0.004142 0.002540 0.014553
None 0.007451 0.000964 0.000235 0.000673 0.000479 0.000911 0.000341 0.001576 0.004337 0.000920 0.000243 0.001255
Nuclear Periphery 0.004916 0.003615 0.004070 0.011387 0.002933 0.002095 0.006019 0.010063 0.004030 0.002357 0.004218 0.010534
Nuclear Periphery Foci 0.003793 0.002637 0.001713 0.005870 0.006133 0.002654 0.002575 0.007290 0.004838 0.002651 0.001778 0.006785
Nucleolus 0.541090* 0.645710* 0.711025* 0.725644* 0.738859* 0.567660* 0.723903* 0.668301* 0.629415* 0.581094* 0.712001* 0.688705*
Nucleus 0.420223* 0.319083* 0.259416* 0.160059* 0.205343* 0.400739* 0.232895* 0.223456* 0.324257* 0.386685* 0.257407* 0.200898*
Peroxisomes 0.004434 0.019033 0.012825 0.032753 0.032925 0.007983 0.023806 0.024021 0.017158 0.009885 0.013657 0.027128
Vacuole 0.002364 0.000790 0.000561 0.005144 0.000809 0.001413 0.000703 0.007338 0.001669 0.001305 0.000572 0.006557
Vacuole Periphery 0.004029 0.001435 0.002074 0.009113 0.001367 0.001802 0.001496 0.015868 0.002840 0.001739 0.002031 0.013465

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.41 4.04 -2.80 -5.38 -5.03 -6.55 -1.77 -9.77 -6.23 -6.14 0.47 0.47 -7.64 -7.95 -8.07
Bud Neck -0.71 -3.99 -11.58 -10.10 -10.25 -6.93 -1.90 -13.16 -8.46 -12.16 -6.97 -4.73 -17.08 -11.09 -15.01
Bud Site 2.59 2.79 0.30 -6.31 -7.60 -2.47 1.64 -6.35 -2.90 -6.69 0.71 2.90 -2.16 -3.64 -8.91
Cell Periphery 3.75 3.93 2.54 -5.59 -6.80 -3.57 2.95 -4.45 -3.14 -4.97 2.23 4.12 -0.66 -3.49 -5.80
Cytoplasm 4.06 4.42 3.62 -1.68 -3.66 -2.89 4.69 -2.29 -1.87 -2.38 2.59 4.56 -0.72 -1.86 -2.55
Cytoplasmic Foci -1.78 1.22 -6.49 -5.31 -6.75 3.11 4.11 -5.51 -6.78 -7.11 0.66 5.36 -8.68 -8.80 -10.10
Eisosomes -0.57 -1.76 -5.23 -3.92 -4.36 4.23 8.16 -1.31 -3.16 -4.75 0.93 2.77 -4.25 -4.44 -5.28
Endoplasmic Reticulum 6.66 9.06 7.24 0.94 -1.71 -3.61 4.64 0.94 4.33 -3.70 4.61 9.95 7.53 4.28 -4.41
Endosome 3.30 3.65 -1.73 -2.09 -2.13 -2.21 2.82 -2.21 -1.79 -2.56 -0.73 4.26 -2.73 -2.58 -3.29
Golgi 1.51 2.05 -2.52 -3.41 -3.65 1.02 1.35 -2.01 -2.33 -2.41 1.54 2.57 -2.57 -2.98 -3.21
Lipid Particles 0.22 3.76 -2.83 -2.27 -5.27 0.88 3.11 -1.71 -2.28 -3.36 0.35 4.57 -2.55 -2.72 -4.37
Mitochondria 2.99 0.19 -8.55 -9.83 -8.78 0.12 0.40 -6.28 -6.79 -6.85 0.70 0.10 -9.50 -9.88 -9.87
Mitotic Spindle 0.16 -3.21 -6.51 -6.54 -5.79 -1.05 0.40 -4.33 -3.21 -3.49 -2.37 -1.07 -8.13 -5.91 -7.41
None 5.69 6.38 5.97 1.62 -7.89 -2.33 1.91 -4.14 -2.12 -4.72 5.28 6.51 4.70 -1.53 -6.03
Nuclear Periphery 0.80 0.86 -2.94 -3.01 -3.30 1.22 -0.97 -4.92 -5.94 -1.17 2.89 -0.21 -5.34 -6.98 -4.69
Nuclear Periphery Foci 1.36 3.55 -1.77 -2.69 -4.40 3.13 2.25 -0.90 -4.76 -3.16 3.29 5.15 -2.23 -5.43 -7.20
Nucleolus -7.43 -20.08* -16.30 -4.79 -0.77 21.23* 0.77 8.50 -10.22 2.86 6.65 -12.01 -7.61 -12.95 3.67
Nucleus 7.10 19.21 26.51 10.62 10.62 -24.72 -1.39 -2.45 19.90 0.44 -8.60 9.73 18.49 24.66 7.75
Peroxisomes -7.87 -15.26 -15.20 -5.55 -10.46 16.59* 3.32 4.20 -10.26 -0.16 8.47 4.01 -7.61 -13.66 -10.70
Vacuole 4.21 4.42 -3.30 -5.49 -5.64 -2.50 0.66 -7.54 -6.55 -7.61 1.14 3.79 -7.55 -7.96 -9.09
Vacuole Periphery 4.97 3.36 -4.38 -7.36 -6.52 -0.92 -0.24 -9.12 -9.04 -9.22 2.74 1.83 -9.57 -10.86 -10.43
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication
Localization
Cell Percentages nucleolus (39%), nucleus (12%), mixed (38%)
Cell Cycle Regulation Yes
nucleus - MA (2e-10)
Subcompartmental Group N/A

Ssf1

Ssf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available