Standard name | |
---|---|
Human Ortholog | |
Description | Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 4 | 1 | 9 | 7 | 9 | 10 | 10 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 9 | 12 |
Bud Neck | 0 | 0 | 2 | 1 | 3 | 1 | 4 | 4 | 1 | 10 | 0 | 7 | 9 | 15 | 11 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 |
Bud Site | 0 | 0 | 2 | 1 | 5 | 4 | 13 | 19 | 13 | 22 | 21 | 30 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 1 | 8 | 15 | 11 | 10 | 13 | 14 | 12 | 5 | 6 | 4 | 46 | 57 | 86 | 10 | 11 | 15 | 0 | 0 | 0 | 1 | 3 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 0 | 1 | 6 | 4 | 21 | 13 | 18 | 35 | 16 | 13 | 21 | 22 | 9 | 14 | 17 | 2 | 2 | 1 | 1 | 0 | 12 | 7 | 16 | 32 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Mitochondria | 32 | 5 | 18 | 5 | 24 | 36 | 70 | 62 | 68 | 100 | 67 | 95 | 3 | 2 | 2 | 11 | 17 | 18 | 1 | 0 | 3 | 7 | 13 | 16 |
Nucleus | 50 | 52 | 37 | 10 | 26 | 18 | 29 | 50 | 30 | 51 | 34 | 65 | 24 | 18 | 24 | 29 | 29 | 26 | 20 | 27 | 16 | 34 | 55 | 39 |
Nuclear Periphery | 8 | 0 | 8 | 2 | 0 | 2 | 2 | 4 | 8 | 5 | 1 | 9 | 0 | 1 | 1 | 1 | 1 | 1 | 6 | 1 | 12 | 6 | 10 | 12 |
Nucleolus | 122 | 86 | 250 | 126 | 254 | 181 | 319 | 372 | 259 | 354 | 296 | 354 | 338 | 452 | 402 | 99 | 160 | 120 | 130 | 107 | 223 | 260 | 371 | 334 |
Peroxisomes | 1 | 0 | 3 | 4 | 5 | 5 | 9 | 12 | 10 | 5 | 9 | 6 | 6 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 |
SpindlePole | 2 | 0 | 21 | 32 | 62 | 68 | 111 | 135 | 110 | 122 | 89 | 132 | 105 | 155 | 137 | 2 | 8 | 0 | 6 | 1 | 40 | 23 | 51 | 49 |
Vac/Vac Membrane | 2 | 2 | 6 | 0 | 4 | 1 | 2 | 6 | 5 | 8 | 5 | 4 | 1 | 5 | 8 | 7 | 11 | 9 | 7 | 7 | 7 | 20 | 41 | 42 |
Unique Cell Count | 173 | 129 | 303 | 150 | 312 | 225 | 404 | 492 | 352 | 483 | 375 | 484 | 408 | 538 | 529 | 141 | 210 | 166 | 175 | 147 | 329 | 373 | 584 | 570 |
Labelled Cell Count | 219 | 149 | 361 | 200 | 420 | 340 | 599 | 720 | 541 | 706 | 559 | 740 | 542 | 723 | 696 | 163 | 240 | 192 | 175 | 147 | 329 | 373 | 584 | 570 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.8 | 17.2 | 14.3 | 12.1 | 9.7 | 8.1 | 7.6 | 7.2 | 6.6 | 6.6 | 6.3 | 6.3 | 14.4 | 13.6 | 12.5 | 20.4 | 20.8 | 23.0 | 16.4 | 15.7 | 16.7 |
Std Deviation (1e-4) | 3.3 | 4.6 | 4.2 | 4.6 | 3.1 | 1.9 | 2.1 | 2.0 | 1.8 | 1.7 | 2.0 | 1.6 | 5.5 | 4.9 | 5.1 | 8.6 | 8.0 | 10.8 | 5.1 | 4.4 | 4.2 |
Intensity Change (Log2) | – | – | – | -0.24 | -0.56 | -0.82 | -0.92 | -0.99 | -1.11 | -1.11 | -1.19 | -1.19 | 0.01 | -0.07 | -0.2 | 0.51 | 0.54 | 0.68 | 0.2 | 0.13 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 2.3 | 2.7 | 2.6 | 3.1 | 3.5 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.4 | 0.6 | 1.1 | 0.4 | 0.2 | 0.6 | -1.7 | -0.9 | 0 | 4.3 | 4.1 | 6.0 | 2.2 | 1.5 | 3.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.9 | 2.3 | 1.8 | 3.2 | 1.8 | 0.6 | 2.4 | 1.9 | 0.2 | 0.6 | 1.0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.2 | 0.9 | 3.8 | 4.5 | 3.0 | 5.1 | 5.6 | 4.7 | 5.3 | -4.1 | -5.1 | -5.0 | 0.7 | 1.0 | 1.9 |
Nucleus | -1.8 | -1.6 | -1.6 | -2.3 | -0.9 | -1.6 | -0.7 | -1.3 | 0.5 | -3.0 | -5.0 | -4.1 | 2.3 | 0.5 | 1.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | -0.3 | -1.7 | 0 | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.4 | -0.4 | -0.6 | -1.2 | -2.3 | -2.7 | -3.0 | -1.2 | -3.0 | 0.1 | 0.6 | -2.2 | -2.9 | -1.8 | -2.6 |
Peroxisomes | 0 | 0 | 0 | 1.3 | 1.5 | 1.7 | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4.5 | 4.7 | 7.1 | 6.9 | 7.1 | 7.8 | 6.5 | 5.9 | 7.0 | 6.5 | 7.5 | 6.7 | -2.4 | -1.5 | -3.5 |
Vacuole | 0 | 0 | 0 | 0 | 0 | -0.6 | -0.3 | 0 | 0 | 0 | 0 | -0.5 | 0 | 2.0 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.0476 | 13.6165 | 13.3824 | 12.5164 | 10.7879 | 12.6022 | 12.3322 | 14.5813 | 12.9655 | 13.018 | 12.513 | 13.1336 | 12.6248 | 13.5803 | 12.2836 | 12.1364 | 11.6154 | 11.9812 |
Actin | 0.0023 | 0 | 0 | 0 | 0.029 | 0 | 0.0019 | 0.0001 | 0.0021 | 0 | 0 | 0.0019 | 0.0094 | 0 | 0.0006 | 0 | 0.0001 | 0 |
Bud | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0024 | 0.0001 | 0.0002 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Periphery | 0.0002 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0005 | 0.0001 | 0 | 0.0002 | 0.0015 | 0.0001 | 0.0005 | 0.0004 | 0.003 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0008 | 0 | 0 | 0 | 0.0129 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0011 | 0 | 0.0002 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0016 | 0.0001 | 0.0003 | 0.0001 | 0.0042 | 0.0008 | 0.0079 | 0.0058 | 0.0027 | 0.0004 | 0.0025 | 0.0012 | 0.0113 | 0.0003 | 0.0013 | 0.0002 | 0.0001 | 0.0003 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0004 | 0 | 0 | 0 | 0.0025 | 0 | 0.0019 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0006 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0028 | 0.0001 | 0 | 0 | 0.0025 | 0.0001 | 0.0029 | 0.0046 | 0.0002 | 0 | 0.0009 | 0.0009 | 0.0039 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Golgi | 0.0005 | 0 | 0 | 0 | 0.0015 | 0.0001 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0013 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Lipid Particles | 0.0027 | 0.0006 | 0.0007 | 0.0003 | 0.021 | 0.0052 | 0.0061 | 0.0048 | 0.0026 | 0.0015 | 0.0055 | 0.005 | 0.0085 | 0.0012 | 0.0043 | 0.0004 | 0.0024 | 0.0034 |
Mitochondria | 0.0021 | 0.0003 | 0.0001 | 0.0001 | 0.017 | 0.002 | 0.0017 | 0.0002 | 0.0009 | 0 | 0.0038 | 0.0041 | 0.0013 | 0.0019 | 0.0002 | 0.0001 | 0.0009 | 0.002 |
None | 0.0029 | 0 | 0 | 0 | 0.0225 | 0 | 0.0008 | 0 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0019 | 0 | 0.0036 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.0134 | 0.001 | 0.0007 | 0.0002 | 0.0886 | 0.0031 | 0.0098 | 0.004 | 0.0001 | 0.0011 | 0.0004 | 0.0128 | 0.0045 | 0.0034 | 0.0003 | 0.0001 | 0.0513 | 0.0004 |
Nucleolus | 0.8379 | 0.8499 | 0.8994 | 0.8874 | 0.5409 | 0.8636 | 0.8376 | 0.8359 | 0.8773 | 0.8413 | 0.8717 | 0.868 | 0.8893 | 0.8894 | 0.9145 | 0.8795 | 0.838 | 0.9019 |
Nucleus | 0.1241 | 0.1396 | 0.0959 | 0.1092 | 0.1987 | 0.117 | 0.1157 | 0.1369 | 0.1061 | 0.1433 | 0.0932 | 0.0917 | 0.0549 | 0.0937 | 0.0688 | 0.1185 | 0.0868 | 0.0843 |
Peroxisomes | 0.0004 | 0 | 0 | 0 | 0.0012 | 0.0001 | 0.0005 | 0.0022 | 0.0003 | 0 | 0.0003 | 0.0003 | 0.0019 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 |
Punctate Nuclear | 0.0036 | 0.0011 | 0.0023 | 0.0009 | 0.0419 | 0.0062 | 0.0066 | 0.0033 | 0.002 | 0.0118 | 0.0119 | 0.0094 | 0.0076 | 0.0012 | 0.0047 | 0.0006 | 0.016 | 0.0061 |
Vacuole | 0.0009 | 0.0048 | 0.0002 | 0.0006 | 0.0014 | 0.0003 | 0.0017 | 0.0004 | 0.0005 | 0.0001 | 0.005 | 0.0007 | 0.0006 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0002 |
Vacuole Periphery | 0.0019 | 0.002 | 0.0002 | 0.0003 | 0.0084 | 0.0008 | 0.0017 | 0.0003 | 0.0006 | 0.0001 | 0.0029 | 0.0024 | 0.0009 | 0.0073 | 0.0002 | 0.0002 | 0.0034 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 52.2537 | 47.798 | 48.9031 | 41.2319 | 56.7203 | 34.0069 | 31.4867 | 28.1705 | 29.6675 | 45.4397 |
Translational Efficiency | 1.7222 | 1.5112 | 1.3107 | 1.3626 | 1.1841 | 1.5199 | 1.5085 | 1.0556 | 1.0749 | 1.2394 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2021 | 442 | 1928 | 802 | 1631 | 2126 | 158 | 1452 | 3652 | 2568 | 2086 | 2254 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 973.28 | 1295.03 | 1525.61 | 1739.78 | 1149.92 | 1301.65 | 1557.17 | 1735.33 | 1052.17 | 1300.51 | 1528.00 | 1736.91 |
Standard Deviation | 234.71 | 302.47 | 277.67 | 370.06 | 236.26 | 253.60 | 320.39 | 487.38 | 251.25 | 262.67 | 281.25 | 449.17 |
Intensity Change Log 2 | — | 0.412059 | 0.648459 | 0.837978 | — | 0.178808 | 0.437393 | 0.593677 | — | 0.290428 | 0.537992 | 0.710818 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000748 | 0.000518 | 0.000577 | 0.000993 | 0.000374 | 0.000574 | 0.000478 | 0.000958 | 0.000581 | 0.000565 | 0.000569 | 0.000971 |
Bud Neck | 0.001531 | 0.001721 | 0.002204 | 0.007761 | 0.001423 | 0.003617 | 0.001859 | 0.008744 | 0.001483 | 0.003291 | 0.002178 | 0.008394 |
Bud Site | 0.001639 | 0.000275 | 0.000186 | 0.001373 | 0.000277 | 0.000804 | 0.000200 | 0.001801 | 0.001031 | 0.000713 | 0.000187 | 0.001648 |
Cell Periphery | 0.000187 | 0.000022 | 0.000015 | 0.000073 | 0.000027 | 0.000066 | 0.000011 | 0.000172 | 0.000116 | 0.000059 | 0.000015 | 0.000137 |
Cytoplasm | 0.001602 | 0.000138 | 0.000018 | 0.000253 | 0.000085 | 0.000366 | 0.000013 | 0.001717 | 0.000925 | 0.000326 | 0.000018 | 0.001197 |
Cytoplasmic Foci | 0.000474 | 0.001396 | 0.000295 | 0.007621 | 0.002103 | 0.000894 | 0.000725 | 0.007419 | 0.001201 | 0.000981 | 0.000327 | 0.007491 |
Eisosomes | 0.000095 | 0.000102 | 0.000107 | 0.000184 | 0.000159 | 0.000120 | 0.000087 | 0.000188 | 0.000124 | 0.000117 | 0.000106 | 0.000187 |
Endoplasmic Reticulum | 0.002362 | 0.000607 | 0.000232 | 0.000436 | 0.000567 | 0.000913 | 0.000249 | 0.000474 | 0.001561 | 0.000860 | 0.000233 | 0.000461 |
Endosome | 0.000399 | 0.000081 | 0.000049 | 0.002073 | 0.000211 | 0.000430 | 0.000030 | 0.001493 | 0.000315 | 0.000370 | 0.000047 | 0.001699 |
Golgi | 0.000278 | 0.000162 | 0.000105 | 0.001003 | 0.000363 | 0.000195 | 0.000160 | 0.001664 | 0.000316 | 0.000189 | 0.000110 | 0.001429 |
Lipid Particles | 0.000119 | 0.000100 | 0.000019 | 0.000289 | 0.000235 | 0.000162 | 0.000026 | 0.000478 | 0.000171 | 0.000151 | 0.000020 | 0.000411 |
Mitochondria | 0.001808 | 0.001223 | 0.001756 | 0.006434 | 0.001733 | 0.001679 | 0.001582 | 0.005910 | 0.001775 | 0.001600 | 0.001743 | 0.006097 |
Mitotic Spindle | 0.000457 | 0.000388 | 0.002515 | 0.020864 | 0.003594 | 0.004923 | 0.002842 | 0.011067 | 0.001858 | 0.004142 | 0.002540 | 0.014553 |
None | 0.007451 | 0.000964 | 0.000235 | 0.000673 | 0.000479 | 0.000911 | 0.000341 | 0.001576 | 0.004337 | 0.000920 | 0.000243 | 0.001255 |
Nuclear Periphery | 0.004916 | 0.003615 | 0.004070 | 0.011387 | 0.002933 | 0.002095 | 0.006019 | 0.010063 | 0.004030 | 0.002357 | 0.004218 | 0.010534 |
Nuclear Periphery Foci | 0.003793 | 0.002637 | 0.001713 | 0.005870 | 0.006133 | 0.002654 | 0.002575 | 0.007290 | 0.004838 | 0.002651 | 0.001778 | 0.006785 |
Nucleolus | 0.541090* | 0.645710* | 0.711025* | 0.725644* | 0.738859* | 0.567660* | 0.723903* | 0.668301* | 0.629415* | 0.581094* | 0.712001* | 0.688705* |
Nucleus | 0.420223* | 0.319083* | 0.259416* | 0.160059* | 0.205343* | 0.400739* | 0.232895* | 0.223456* | 0.324257* | 0.386685* | 0.257407* | 0.200898* |
Peroxisomes | 0.004434 | 0.019033 | 0.012825 | 0.032753 | 0.032925 | 0.007983 | 0.023806 | 0.024021 | 0.017158 | 0.009885 | 0.013657 | 0.027128 |
Vacuole | 0.002364 | 0.000790 | 0.000561 | 0.005144 | 0.000809 | 0.001413 | 0.000703 | 0.007338 | 0.001669 | 0.001305 | 0.000572 | 0.006557 |
Vacuole Periphery | 0.004029 | 0.001435 | 0.002074 | 0.009113 | 0.001367 | 0.001802 | 0.001496 | 0.015868 | 0.002840 | 0.001739 | 0.002031 | 0.013465 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 4.41 | 4.04 | -2.80 | -5.38 | -5.03 | -6.55 | -1.77 | -9.77 | -6.23 | -6.14 | 0.47 | 0.47 | -7.64 | -7.95 | -8.07 |
Bud Neck | -0.71 | -3.99 | -11.58 | -10.10 | -10.25 | -6.93 | -1.90 | -13.16 | -8.46 | -12.16 | -6.97 | -4.73 | -17.08 | -11.09 | -15.01 |
Bud Site | 2.59 | 2.79 | 0.30 | -6.31 | -7.60 | -2.47 | 1.64 | -6.35 | -2.90 | -6.69 | 0.71 | 2.90 | -2.16 | -3.64 | -8.91 |
Cell Periphery | 3.75 | 3.93 | 2.54 | -5.59 | -6.80 | -3.57 | 2.95 | -4.45 | -3.14 | -4.97 | 2.23 | 4.12 | -0.66 | -3.49 | -5.80 |
Cytoplasm | 4.06 | 4.42 | 3.62 | -1.68 | -3.66 | -2.89 | 4.69 | -2.29 | -1.87 | -2.38 | 2.59 | 4.56 | -0.72 | -1.86 | -2.55 |
Cytoplasmic Foci | -1.78 | 1.22 | -6.49 | -5.31 | -6.75 | 3.11 | 4.11 | -5.51 | -6.78 | -7.11 | 0.66 | 5.36 | -8.68 | -8.80 | -10.10 |
Eisosomes | -0.57 | -1.76 | -5.23 | -3.92 | -4.36 | 4.23 | 8.16 | -1.31 | -3.16 | -4.75 | 0.93 | 2.77 | -4.25 | -4.44 | -5.28 |
Endoplasmic Reticulum | 6.66 | 9.06 | 7.24 | 0.94 | -1.71 | -3.61 | 4.64 | 0.94 | 4.33 | -3.70 | 4.61 | 9.95 | 7.53 | 4.28 | -4.41 |
Endosome | 3.30 | 3.65 | -1.73 | -2.09 | -2.13 | -2.21 | 2.82 | -2.21 | -1.79 | -2.56 | -0.73 | 4.26 | -2.73 | -2.58 | -3.29 |
Golgi | 1.51 | 2.05 | -2.52 | -3.41 | -3.65 | 1.02 | 1.35 | -2.01 | -2.33 | -2.41 | 1.54 | 2.57 | -2.57 | -2.98 | -3.21 |
Lipid Particles | 0.22 | 3.76 | -2.83 | -2.27 | -5.27 | 0.88 | 3.11 | -1.71 | -2.28 | -3.36 | 0.35 | 4.57 | -2.55 | -2.72 | -4.37 |
Mitochondria | 2.99 | 0.19 | -8.55 | -9.83 | -8.78 | 0.12 | 0.40 | -6.28 | -6.79 | -6.85 | 0.70 | 0.10 | -9.50 | -9.88 | -9.87 |
Mitotic Spindle | 0.16 | -3.21 | -6.51 | -6.54 | -5.79 | -1.05 | 0.40 | -4.33 | -3.21 | -3.49 | -2.37 | -1.07 | -8.13 | -5.91 | -7.41 |
None | 5.69 | 6.38 | 5.97 | 1.62 | -7.89 | -2.33 | 1.91 | -4.14 | -2.12 | -4.72 | 5.28 | 6.51 | 4.70 | -1.53 | -6.03 |
Nuclear Periphery | 0.80 | 0.86 | -2.94 | -3.01 | -3.30 | 1.22 | -0.97 | -4.92 | -5.94 | -1.17 | 2.89 | -0.21 | -5.34 | -6.98 | -4.69 |
Nuclear Periphery Foci | 1.36 | 3.55 | -1.77 | -2.69 | -4.40 | 3.13 | 2.25 | -0.90 | -4.76 | -3.16 | 3.29 | 5.15 | -2.23 | -5.43 | -7.20 |
Nucleolus | -7.43 | -20.08* | -16.30 | -4.79 | -0.77 | 21.23* | 0.77 | 8.50 | -10.22 | 2.86 | 6.65 | -12.01 | -7.61 | -12.95 | 3.67 |
Nucleus | 7.10 | 19.21 | 26.51 | 10.62 | 10.62 | -24.72 | -1.39 | -2.45 | 19.90 | 0.44 | -8.60 | 9.73 | 18.49 | 24.66 | 7.75 |
Peroxisomes | -7.87 | -15.26 | -15.20 | -5.55 | -10.46 | 16.59* | 3.32 | 4.20 | -10.26 | -0.16 | 8.47 | 4.01 | -7.61 | -13.66 | -10.70 |
Vacuole | 4.21 | 4.42 | -3.30 | -5.49 | -5.64 | -2.50 | 0.66 | -7.54 | -6.55 | -7.61 | 1.14 | 3.79 | -7.55 | -7.96 | -9.09 |
Vacuole Periphery | 4.97 | 3.36 | -4.38 | -7.36 | -6.52 | -0.92 | -0.24 | -9.12 | -9.04 | -9.22 | 2.74 | 1.83 | -9.57 | -10.86 | -10.43 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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