Standard name
Human Ortholog
Description Member of an oxysterol-binding protein family; this family has seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; contains FFAT motif; interacts with ER anchor Scs2p at patches at the plasma membrane; regulated by sterol binding
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.37 0.3 0.3 0.27 0.25 0.2 0.16 0.13 0.12 – 0.15 0.14 0.11 0.05 0 0.22 0.18 0.22 0.32 0.27 0.34 0.23 0.25 0.25
Bud 0.08 0.06 0.11 0.12 0.13 0.12 0.09 0.08 0.09 – 0.07 0.08 0.05 0 0.06 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.13 0.08 0.08 0.08 0.1
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.48 0.52 0.29 0.44 0.28 0.22 0.2 0.13 0.19 – 0.24 0.2 0.34 0.29 0.32 0.27 0.38 0.32 0.42 0.4 0.37 0.43 0.48 0.45
Cytoplasm 0.29 0.36 0.53 0.39 0.37 0.32 0.3 0.36 0.25 – 0.21 0.2 0.83 0.88 0.94 0.56 0.51 0.56 0.05 0.08 0.06 0.08 0.07 0.06
Endoplasmic Reticulum 0.09 0.11 0.05 0 0.13 0.05 0.1 0.06 0.09 – 0.09 0.1 0 0 0 0.05 0 0.07 0 0 0.05 0.07 0.06 0.05
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.21 0.16 0.45 0.46 0.51 0.58 – 0.67 0.7 0 0 0 0.05 0 0 0 0 0.06 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.08 0.07 0.07 0 0.06 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 99 104 77 77 78 94 96 81 57 – 47 58 26 17 11 61 34 56 107 120 116 55 74 88
Bud 20 21 29 34 40 55 52 48 43 – 23 34 13 6 22 9 6 6 3 2 0 1 1 5
Bud Neck 0 0 3 0 1 0 0 0 0 – 0 0 0 0 0 1 0 0 24 60 26 18 22 35
Bud Site 0 1 1 0 5 3 7 6 3 – 2 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 129 182 73 125 89 105 119 80 89 – 73 83 83 93 117 75 71 80 138 181 124 103 143 162
Cytoplasm 77 126 134 110 116 148 173 223 115 – 66 81 201 282 342 159 95 140 16 34 19 20 21 20
Endoplasmic Reticulum 25 37 12 7 40 25 61 34 41 – 28 41 6 11 0 13 5 17 14 19 17 15 17 18
Endosome 0 0 2 0 8 12 3 8 1 – 1 1 2 0 0 3 1 2 4 5 2 1 0 3
Golgi 3 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 5 2 0 0 2 3
Mitochondria 5 10 11 60 52 209 271 311 270 – 205 290 3 1 9 15 6 8 6 9 18 11 9 14
Nucleus 1 1 0 0 0 0 2 3 1 – 0 0 1 0 0 2 3 1 0 0 0 1 0 0
Nuclear Periphery 1 0 0 0 0 1 1 0 0 – 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 1 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 8 24 31 42 24 28 – 14 6 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 4 3 8 4 9 6 8 5 9 – 4 2 0 0 0 7 6 6 1 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 8 10 6 20 10 – 3 2 0 0 0 3 2 1 4 13 5 4 4 4
Unique Cell Count 266 352 254 284 317 469 586 615 463 307 412 242 319 364 283 187 250 332 454 339 238 301 360
Labelled Cell Count 364 486 353 426 470 700 842 843 667 466 605 335 410 501 348 229 319 332 454 339 238 301 360


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 6.8 6.6 5.7 5.8 5.2 4.9 4.9 5.3 – 4.6 4.3 6.4 6.3 5.7 7.3 8.3 8.2 6.8 6.8 7.5
Std Deviation (1e-4) 1.0 0.8 3.2 1.2 2.0 1.2 0.9 1.3 4.8 – 2.2 0.9 0.9 0.9 0.8 1.1 1.4 1.4 1.4 1.2 1.3
Intensity Change (Log2) – – – -0.2 -0.18 -0.33 -0.42 -0.41 -0.32 – -0.51 -0.61 -0.04 -0.07 -0.2 0.16 0.33 0.31 0.05 0.05 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.8 -1.5 -3.1 -4.6 -6.0 -5.9 – 0 0 -5.4 -8.0 -9.6 -2.3 -2.9 -2.0
Bud 0.2 0.4 0.1 -1.1 -1.7 -0.9 – 0 0 -2.4 -4.7 -2.4 -3.7 -3.2 -4.0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 3.7 -0.2 -1.9 -2.7 -5.5 -2.9 – 0 0 1.3 0.1 0.9 -0.6 2.0 0.8
Cytoplasm -3.3 -3.9 -5.6 -6.4 -4.5 -7.5 – -7.7 -8.9 7.2 9.5 12.0 0.8 -0.4 0.7
Endoplasmic Reticulum -1.4 3.3 0.4 2.7 0.5 2.0 – 0 0 -1.3 -0.8 0 -0.1 -1.1 1.0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 5.7 4.6 11.2 11.8 12.8 14.2 – 0 0 -2.1 -3.3 -1.3 0.5 -0.6 -0.7
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 4.5 4.2 4.4 3.2 4.0 – 0 0 0 0 0 0 0 0
SpindlePole -1.4 -0.2 0 0 0 -1.0 – 0 0 0 0 0 -0.5 0 -0.5
Vacuole 0 0 0 0 2.5 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0503 4.0764 4.0415 3.6457 3.8361 4.1977 5.2744 5.6167 5.7762 5.1095 5.7013 5.6989 6.329 6.6474 6.2372 6.0348 6.278 6.1473
Actin 0.1525 0.0946 0.149 0.0472 0.1046 0.1014 0.0614 0.0653 0.1191 0.0673 0.1231 0.0665 0.1119 0.0642 0.1537 0.1964 0.1056 0.0968
Bud 0.0017 0.0012 0.0026 0.0046 0.0024 0.0017 0.0016 0.0089 0.0033 0.0076 0.0038 0.0024 0.0014 0.0012 0.0037 0.0042 0.0018 0.0021
Bud Neck 0.0164 0.0176 0.0694 0.0735 0.05 0.0991 0.0142 0.0112 0.0349 0.0297 0.0299 0.0513 0.0141 0.0091 0.0268 0.012 0.0208 0.0665
Bud Periphery 0.0103 0.0042 0.021 0.0385 0.0283 0.0149 0.0079 0.0066 0.0176 0.0492 0.0303 0.0172 0.0077 0.005 0.0265 0.0534 0.0131 0.0142
Bud Site 0.0419 0.2145 0.0784 0.0431 0.0079 0.0068 0.0255 0.2098 0.0825 0.024 0.0237 0.0057 0.0494 0.2533 0.0614 0.0066 0.0069 0.0076
Cell Periphery 0.2382 0.1823 0.1974 0.1844 0.2169 0.2543 0.2227 0.1225 0.1276 0.149 0.1373 0.1731 0.2443 0.1435 0.1698 0.2199 0.2014 0.2312
Cytoplasm 0.3461 0.4391 0.4183 0.5465 0.4596 0.4508 0.5664 0.5361 0.5332 0.6115 0.5392 0.6384 0.4966 0.4707 0.502 0.4525 0.5542 0.5328
Cytoplasmic Foci 0.0191 0.0047 0.0047 0.0049 0.0108 0.0063 0.0081 0.0039 0.0086 0.016 0.015 0.005 0.0047 0.006 0.0047 0.0048 0.0179 0.0079
Eisosomes 0.0413 0.0097 0.0134 0.0044 0.0121 0.0194 0.009 0.0041 0.0075 0.0016 0.0076 0.006 0.0126 0.0078 0.0059 0.0078 0.0053 0.0068
Endoplasmic Reticulum 0.0157 0.0078 0.0074 0.0186 0.0042 0.0053 0.0107 0.0029 0.0042 0.008 0.0093 0.0045 0.0063 0.0039 0.0085 0.013 0.0067 0.0052
Endosome 0.0187 0.001 0.0066 0.0015 0.0256 0.0031 0.0102 0.0004 0.0061 0.0008 0.0279 0.0026 0.0032 0.001 0.002 0.001 0.0139 0.0009
Golgi 0.0111 0.0055 0.0044 0.0029 0.039 0.0047 0.0082 0.0025 0.0054 0.0035 0.0205 0.0044 0.0049 0.0027 0.0067 0.006 0.0088 0.004
Lipid Particles 0.0092 0.0021 0.0023 0.0039 0.0101 0.0028 0.0058 0.0007 0.0023 0.0013 0.0051 0.0008 0.0039 0.0011 0.002 0.0036 0.0067 0.0012
Mitochondria 0.0044 0.0002 0.0005 0.0004 0.0075 0.0017 0.0033 0.0101 0.0035 0.0004 0.0059 0.002 0.0004 0.0001 0.0068 0.0002 0.0009 0.0006
None 0.0307 0.0133 0.0204 0.0218 0.006 0.0202 0.0297 0.0122 0.0356 0.0173 0.0083 0.017 0.0328 0.0281 0.0155 0.016 0.0264 0.0187
Nuclear Periphery 0.0108 0.0004 0.0008 0.0004 0.0072 0.0019 0.0042 0.0002 0.0004 0.0009 0.0034 0.0002 0.0007 0.0003 0.0003 0.0004 0.0007 0.0002
Nucleolus 0.0012 0.0001 0.0003 0.0002 0.0004 0.0004 0.0004 0.0002 0.0003 0.0006 0.0005 0.0001 0.0006 0.0001 0.0002 0.0001 0.0004 0.0002
Nucleus 0.0166 0.0004 0.0006 0.0005 0.0011 0.0017 0.0033 0.0006 0.0009 0.0015 0.002 0.0006 0.0011 0.0003 0.0004 0.0003 0.0006 0.0005
Peroxisomes 0.0052 0.0002 0.0006 0.0001 0.0015 0.0009 0.0026 0.0005 0.0023 0.0001 0.0016 0.0007 0.0007 0.0003 0.001 0.0001 0.0034 0.0003
Punctate Nuclear 0.0041 0.0002 0.0005 0.0002 0.002 0.0012 0.0013 0.0004 0.0003 0.0067 0.0021 0.0002 0.0007 0.0005 0.0003 0.0002 0.0027 0.0007
Vacuole 0.004 0.0011 0.0013 0.0023 0.0018 0.0015 0.0028 0.0011 0.0039 0.0029 0.0028 0.0011 0.002 0.0007 0.0016 0.0015 0.0018 0.0014
Vacuole Periphery 0.0008 0 0.0001 0.0001 0.001 0.0003 0.0006 0 0.0005 0.0002 0.0007 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.6391 28.0314 19.3323 30.2265 29.1766 26.178 30.8434 29.4816 32.6579 30.4982
Translational Efficiency 0.4676 0.3757 0.588 0.3623 0.326 0.4696 0.3982 0.3578 0.3734 0.3784

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Member of an oxysterol-binding protein family; this family has seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; contains FFAT motif; interacts with ER anchor Scs2p at patches at the plasma membrane; regulated by sterol binding
Localization
Cell Percentages cell periphery (17%), cytoplasm (7%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Osh3

Osh3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Osh3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available