Standard name
Human Ortholog
Description Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0 0.06 – 0.07 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.97 0.99 0.98 0.94 0.9 0.81 0.83 – 0.77 0.78 0.99 0.98 0.98 0.94 0.93 0.95 0.91 0.96 0.92 0.88 0.88 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.06 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0.11 0.18 – 0.27 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 – 0 0.05 0 0 0 0.06 0 0 0 0 0 0.05 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 1 4 2 – 4 1 0 0 1 0 1 0 2 0 1 3 2 3
Bud 0 0 3 0 1 2 0 1 1 – 3 3 4 0 16 0 0 0 0 0 0 3 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 1
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 3 3 0 3 12 20 23 24 – 15 9 9 12 42 22 12 13 0 0 0 0 0 0
Cytoplasm 363 337 346 263 279 375 376 472 311 – 168 219 761 913 946 497 197 258 349 322 345 501 508 595
Endoplasmic Reticulum 7 6 6 5 6 17 25 34 11 – 5 7 3 10 7 13 10 5 4 0 5 15 17 22
Endosome 2 0 0 0 0 0 1 0 0 – 0 0 0 1 0 2 1 0 4 1 3 6 10 14
Golgi 3 1 0 0 0 0 0 0 0 – 0 0 1 2 2 1 1 2 5 1 0 3 4 2
Mitochondria 1 0 2 3 5 16 24 64 68 – 60 61 3 0 6 3 1 4 6 0 4 3 4 2
Nucleus 2 1 1 0 0 1 0 1 0 – 1 0 0 2 4 5 7 10 0 0 0 1 0 0
Nuclear Periphery 0 2 0 0 0 0 0 4 0 – 1 2 0 1 1 0 0 1 2 1 0 1 0 3
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 1 1 0 0 0 1 37 5 – 4 13 4 2 2 29 9 2 2 4 9 25 21 36
Unique Cell Count 375 346 356 267 285 397 418 585 376 219 282 770 927 962 526 212 271 383 335 374 571 579 694
Labelled Cell Count 387 351 362 271 295 424 448 640 422 261 315 785 943 1027 572 239 295 383 335 374 571 579 694


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 7.9 5.9 6.2 9.0 9.4 9.9 10.0 8.8 – 9.0 8.7 8.8 9.2 8.3 11.1 11.6 11.5 7.8 8.3 9.0
Std Deviation (1e-4) 1.0 1.0 0.7 0.9 1.2 1.5 1.8 2.2 2.2 – 2.7 2.8 1.2 1.3 1.2 1.8 2.2 2.3 1.2 1.4 1.6
Intensity Change (Log2) – – – 0.06 0.61 0.67 0.73 0.76 0.57 – 0.6 0.55 0.57 0.63 0.49 0.91 0.96 0.95 0.4 0.49 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 1.1 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.1 3.2 2.8 4.0 – 4.0 2.2 0 0 3.1 2.9 3.5 3.1
Cytoplasm 1.1 0.6 -1.9 -4.0 -7.3 -6.5 – -7.7 -7.7 2.0 1.5 1.3 -1.9 -2.4 -1.3
Endoplasmic Reticulum 0 0.4 2.1 3.0 3.0 1.1 – 0 0.7 0 0 0 0.8 2.1 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 4.0 6.0 8.1 – 10.1 8.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.6 0 – 0 3.7 0 0 0 4.2 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7093 6.655 6.1558 6.2816 6.5368 6.0436 3.4831 4.7199 4.6285 4.3034 4.4898 4.6821 9.2831 9.5459 10.0246 9.613 9.9471 9.5446
Actin 0.0268 0.0004 0.0197 0.0932 0.0575 0.0001 0.0093 0.0006 0.001 0.0001 0 0.0001 0.0014 0.002 0.0014 0 0.0001 0.0005
Bud 0.001 0.0011 0.0007 0.0027 0.0069 0.0004 0.0004 0.0021 0.0005 0.0006 0.0001 0.0003 0.0028 0.0094 0.024 0.0002 0.0026 0.0039
Bud Neck 0.003 0.0002 0.0015 0.0014 0.0046 0.0007 0.0004 0.0003 0.0004 0.0007 0.0002 0.0007 0.0004 0.0004 0.0043 0.0003 0.0002 0.0007
Bud Periphery 0.0012 0.0002 0.0005 0.0035 0.0078 0.0008 0.0003 0.0013 0.0002 0.0001 0 0.0001 0.0008 0.0021 0.0074 0 0.0006 0.0009
Bud Site 0.0066 0.0082 0.0027 0.0058 0.0065 0.0001 0.0006 0.0059 0.0008 0.0004 0 0.0001 0.0029 0.0076 0.0156 0.0001 0.0001 0.0001
Cell Periphery 0.001 0.0004 0.0006 0.0004 0.0013 0.0001 0.0003 0.0006 0.0001 0 0 0 0.0002 0.0002 0.0004 0 0.0001 0
Cytoplasm 0.5956 0.9623 0.8625 0.7447 0.5844 0.9226 0.8453 0.9194 0.8954 0.8651 0.9271 0.9103 0.8863 0.8487 0.8307 0.8429 0.9045 0.9313
Cytoplasmic Foci 0.0273 0.0046 0.0255 0.0178 0.0345 0.0072 0.0152 0.0095 0.0156 0.0165 0.0127 0.014 0.0161 0.0162 0.0206 0.02 0.0249 0.0138
Eisosomes 0.0003 0 0.0001 0.0004 0.0002 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0591 0.0026 0.0102 0.004 0.0213 0.0178 0.0325 0.0085 0.0086 0.0029 0.003 0.0148 0.0058 0.0026 0.0036 0.0037 0.0015 0.0029
Endosome 0.0903 0.0041 0.033 0.0459 0.1362 0.0279 0.0399 0.0175 0.0364 0.063 0.0363 0.032 0.0284 0.0324 0.0405 0.0519 0.0431 0.0308
Golgi 0.006 0.0003 0.0077 0.0219 0.0595 0.0005 0.0018 0.001 0.0012 0.0003 0.0003 0.0006 0.0013 0.0013 0.0036 0.0003 0.0005 0.0008
Lipid Particles 0.0055 0.0001 0.0038 0.0038 0.0077 0.0001 0.0007 0.0003 0.0002 0 0 0.0001 0.0003 0.0002 0.0004 0 0 0
Mitochondria 0.0044 0.0001 0.002 0.0166 0.0173 0.0012 0.0016 0.001 0.0004 0.0002 0.0003 0.0004 0.0011 0.0021 0.0023 0.0002 0.0002 0.0006
None 0.0265 0.008 0.007 0.009 0.0026 0.0023 0.0166 0.0114 0.0179 0.0021 0.0017 0.0094 0.03 0.056 0.018 0.042 0.0056 0.0043
Nuclear Periphery 0.0476 0.0006 0.0018 0.0015 0.0182 0.0034 0.0051 0.0024 0.0012 0.0014 0.0009 0.0017 0.0013 0.0015 0.0016 0.0009 0.0005 0.0006
Nucleolus 0.0017 0.0001 0.0001 0.0001 0.0003 0 0.0001 0.0002 0 0 0 0 0.0001 0.0002 0.0008 0 0 0
Nucleus 0.0348 0.001 0.0014 0.001 0.0036 0.0019 0.0029 0.0048 0.0017 0.0025 0.0012 0.002 0.0017 0.0034 0.0044 0.0019 0.001 0.0019
Peroxisomes 0.0025 0 0.0091 0.0028 0.0028 0 0.0004 0.0001 0.0001 0 0 0 0.0003 0.0002 0.0011 0 0.0001 0.0001
Punctate Nuclear 0.0214 0.0002 0.0009 0.0005 0.0029 0.0001 0.0013 0.0007 0.0003 0.0002 0 0.0003 0.0004 0.0014 0.0015 0.0002 0.0001 0.0002
Vacuole 0.0317 0.0047 0.0079 0.0184 0.0187 0.0101 0.0222 0.0106 0.0158 0.0353 0.0137 0.0111 0.0163 0.0101 0.0153 0.0295 0.0112 0.005
Vacuole Periphery 0.0056 0.0005 0.0015 0.0044 0.0053 0.0029 0.0031 0.0014 0.0022 0.0085 0.0024 0.0019 0.002 0.0022 0.0026 0.0056 0.0031 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 57.1342 46.6055 32.9252 55.8535 37.27 38.5442 52.529 57.0742 49.0662 60.6749
Translational Efficiency 0.5944 0.7151 0.7614 0.4491 0.6647 0.9968 0.6638 0.5061 0.6119 0.5336

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1740 128 215 360 1483 1616 300 113 3223 1744 515 473

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 752.52 986.17 1320.22 1423.38 831.68 855.65 1221.47 1350.79 788.94 865.23 1262.70 1406.04
Standard Deviation 116.59 149.03 151.51 233.64 107.79 121.94 191.92 258.93 119.34 128.71 182.79 241.92
Intensity Change Log 2 — 0.390106 0.810977 0.919519 — 0.040992 0.554518 0.699703 — 0.217378 0.682034 0.808302

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000139 0.000402 0.000449 0.000274 0.000183 0.000234 0.000204 0.000286 0.000159 0.000246 0.000307 0.000277
Bud Neck 0.006902 0.009100 0.003240 0.003042 0.005668 0.006377 0.003349 0.002654 0.006334 0.006577 0.003303 0.002950
Bud Site 0.014031 0.035162 0.023736 0.025455 0.016482 0.019365 0.011257 0.012624 0.015159 0.020525 0.016467 0.022389
Cell Periphery 0.000187 0.000127 0.000154 0.000549 0.000177 0.000090 0.000152 0.000470 0.000182 0.000092 0.000153 0.000530
Cytoplasm 0.599704* 0.440138* 0.522319* 0.644418* 0.587520* 0.487868* 0.475660* 0.524855* 0.594098* 0.484365* 0.495139* 0.615855*
Cytoplasmic Foci 0.175673 0.270083* 0.120253 0.036802 0.213037* 0.169473 0.046000 0.041245 0.192865* 0.176857 0.076999 0.037863
Eisosomes 0.000071 0.000053 0.000014 0.000004 0.000067 0.000036 0.000028 0.000006 0.000069 0.000037 0.000022 0.000005
Endoplasmic Reticulum 0.002980 0.000711 0.001316 0.001171 0.003885 0.002067 0.000953 0.000298 0.003397 0.001968 0.001104 0.000963
Endosome 0.025900 0.064335 0.030607 0.009043 0.029080 0.045014 0.011888 0.008913 0.027363 0.046432 0.019703 0.009012
Golgi 0.008094 0.012391 0.001711 0.002538 0.009910 0.006662 0.000254 0.000499 0.008929 0.007082 0.000862 0.002051
Lipid Particles 0.003766 0.001492 0.003434 0.003610 0.003253 0.003072 0.001481 0.001952 0.003530 0.002956 0.002297 0.003214
Mitochondria 0.001665 0.004771 0.000027 0.000046 0.002076 0.002786 0.000038 0.002663 0.001854 0.002932 0.000034 0.000671
Mitotic Spindle 0.001041 0.004174 0.004508 0.004488 0.000531 0.007764 0.000487 0.005318 0.000806 0.007500 0.002165 0.004686
None 0.005729 0.000078 0.000108 0.000052 0.000915 0.000829 0.001669 0.000089 0.003514 0.000774 0.001018 0.000061
Nuclear Periphery 0.000137 0.000044 0.000222 0.000106 0.000048 0.000204 0.000220 0.000634 0.000096 0.000193 0.000220 0.000232
Nuclear Periphery Foci 0.000566 0.001468 0.005617 0.001442 0.000290 0.000261 0.003543 0.001630 0.000439 0.000349 0.004409 0.001487
Nucleolus 0.000865 0.000175 0.000105 0.000270 0.000226 0.000407 0.000331 0.000319 0.000571 0.000390 0.000236 0.000282
Nucleus 0.020080 0.003317 0.006777 0.002508 0.008826 0.015318 0.045912 0.006359 0.014902 0.014438 0.029574 0.003428
Peroxisomes 0.001293 0.003623 0.000930 0.001478 0.001416 0.001997 0.000663 0.000988 0.001349 0.002117 0.000774 0.001361
Vacuole 0.123907 0.141982 0.270355* 0.256251* 0.108935 0.219870* 0.394825* 0.384270* 0.117018 0.214154* 0.342862* 0.286835*
Vacuole Periphery 0.007270 0.006373 0.004119 0.006451 0.007475 0.010305 0.001088 0.003927 0.007365 0.010016 0.002353 0.005848

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.28 -4.23 -2.63 0.98 2.11 -0.79 -1.54 -0.85 -0.36 0.06 -1.54 -3.98 -2.51 -0.51 1.23
Bud Neck -1.24 6.40 8.35 3.46 1.16 -1.25 3.76 4.34 4.89 0.89 -0.48 7.01 9.35 6.78 1.28
Bud Site -3.41 -3.99 -2.98 1.54 -0.14 -1.53 0.90 0.96 1.69 0.23 -3.30 -1.65 -2.36 -0.40 -1.10
Cell Periphery 3.27 1.28 -4.01 -4.68 -4.22 6.98 1.08 -1.53 -2.13 -1.71 10.41 1.61 -4.27 -5.56 -4.57
Cytoplasm 6.42 3.97 -2.71 -7.14 -5.05 10.26 4.16 2.64 -0.89 -0.05 13.34 5.86 -1.32 -8.40 -5.27
Cytoplasmic Foci -5.67 7.20 27.68 14.13 10.33 7.06 27.91 24.14 18.30 0.69 3.17 23.33 38.06 27.48 7.82
Eisosomes 1.89 12.24 15.29 5.49 6.91 6.73 7.56 15.40 8.99 3.53 8.07 12.52 22.06 11.54 5.45
Endoplasmic Reticulum 4.92 3.15 2.75 -0.48 0.35 2.22 4.44 5.33 3.75 4.11 2.50 5.76 4.33 1.77 0.36
Endosome -4.18 -0.78 12.21 6.12 6.91 -7.58 13.48 12.79 17.91 0.46 -9.94 6.67 17.23 20.11 5.78
Golgi -0.86 13.84 7.46 2.06 -1.11 3.90 18.45 17.71 9.18 -2.12 2.42 23.63 11.78 5.98 -2.09
Lipid Particles 6.76 -0.27 -0.07 -3.99 0.13 0.49 5.14 2.05 1.42 -1.13 1.67 3.35 0.20 -0.95 -1.74
Mitochondria -1.01 8.53 8.42 1.55 -0.51 -1.07 5.78 -0.01 0.31 -0.99 -1.82 9.46 2.46 3.20 -1.00
Mitotic Spindle -0.78 -2.06 -1.17 -0.09 0.15 -4.17 -1.22 -0.89 0.82 -0.77 -3.98 -2.15 -1.53 1.13 -0.73
None 5.30 5.27 5.31 0.34 1.16 0.24 -0.43 4.52 2.28 1.46 4.19 3.64 5.90 2.32 1.50
Nuclear Periphery 2.25 -1.91 0.57 -1.24 2.31 -4.39 -7.65 -1.03 -0.69 -0.52 -2.44 -5.03 -0.91 -0.09 0.60
Nuclear Periphery Foci -1.00 -10.23 -3.54 -0.09 7.80 0.95 -8.30 -3.65 -3.71 4.38 1.20 -12.66 -5.08 -5.32 8.51
Nucleolus 6.02 7.13 5.48 -1.49 -3.89 -5.15 -1.43 -1.61 0.48 -0.76 2.77 5.64 4.31 2.22 -1.60
Nucleus 11.73 6.96 11.75 -0.16 4.12 -5.33 -6.52 0.89 3.43 6.38 0.39 -4.13 11.11 8.26 7.49
Peroxisomes -4.87 2.80 -0.36 4.26 -1.93 -3.58 4.44 1.98 3.99 -1.29 -4.98 5.00 0.31 3.54 -2.57
Vacuole -1.40 -8.37 -9.52 -6.17 0.85 -16.66 -14.51 -10.29 -6.21 -1.13 -16.16 -16.31 -13.56 -5.60 1.89
Vacuole Periphery 0.44 4.08 1.00 0.23 -1.38 -2.90 11.61 4.43 6.44 -3.29 -3.15 10.73 1.88 3.61 -2.77
Standard name
Human Ortholog
Description Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Ksp1

Ksp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ksp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available