Standard name
Human Ortholog
Description RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.05 0.05 0 0.05 0.08 0.1 0.06 0.05 0 0.07 0.05 0.09 0.13 0.25 0.23 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.07 0 0.18 0.22 0.06 0.32 0.29 0.32 0.49 0.49 0.49 0.56 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.77 0.49 0.75 0.81 0.71 0.55 0.6 0.64 0.66 0.63 0.61 0.62 0.63 0.51 0.53 – – – 0.74 0.72 0.69 0.66 0.54 0.44
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.08 0.13 0.07 0 0 0 0 0 0 0.05 0.06 0 0 0 0 – – – 0.13 0.1 0.08 0.13 0.14 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.2 0.39 0.12 0.1 0.18 0.23 0.18 0.12 0.07 0.08 0.09 0.05 0.22 0.24 0.23 – – – 0 0.07 0.09 0.07 0.12 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – 0 0 0 0 5 2
Bud 0 0 1 1 0 1 2 3 8 4 1 3 0 0 0 – – – 2 7 5 4 15 26
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 2 1 0 3 5
Bud Site 0 0 0 0 0 1 2 6 9 8 4 5 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 – – – 0 0 0 0 0 0
Cytoplasm 3 11 6 6 17 24 18 18 10 26 7 17 22 110 93 – – – 1 2 3 2 8 12
Endoplasmic Reticulum 0 0 1 0 1 0 1 0 0 0 1 0 1 0 0 – – – 0 0 1 1 5 3
Endosome 0 3 0 0 2 1 2 3 0 0 0 0 2 6 8 – – – 1 6 5 5 19 19
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 – – – 0 1 0 0 2 4
Mitochondria 4 7 26 28 12 73 82 123 131 171 69 107 2 3 3 – – – 0 1 1 1 2 6
Nucleus 46 101 109 103 154 127 169 245 176 222 86 118 107 224 211 – – – 70 189 118 123 265 193
Nuclear Periphery 0 0 0 0 0 0 1 0 1 1 2 1 0 0 0 – – – 0 0 1 0 5 4
Nucleolus 5 27 10 5 1 4 8 16 10 17 8 8 5 5 12 – – – 12 27 13 24 66 53
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – – – 3 5 2 4 21 13
Vac/Vac Membrane 12 79 18 13 39 52 51 45 20 27 12 10 37 104 91 – – – 3 18 14 13 58 88
Unique Cell Count 60 205 146 127 217 230 280 381 267 351 141 190 170 436 398 96 264 173 188 488 444
Labelled Cell Count 70 228 171 156 226 283 338 459 365 476 190 269 177 455 418 96 264 173 188 488 444


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 9.4 6.8 6.5 7.5 6.3 6.2 6.0 5.5 5.5 5.2 5.2 10.2 10.1 9.8 – – – 9.0 8.9 9.4
Std Deviation (1e-4) 1.0 1.6 1.4 1.2 1.6 1.4 1.6 1.3 1.2 1.4 1.0 1.4 1.9 2.3 2.4 – – – 1.4 1.7 1.6
Intensity Change (Log2) – – – -0.07 0.13 -0.13 -0.14 -0.19 -0.3 -0.32 -0.41 -0.39 0.58 0.57 0.53 – – – 0.4 0.38 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0.2 1.4 2.2 1.0 0.3 -0.2 1.4 0.3 1.7 2.8 5.5 5.2 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0.9 -3.7 3.0 2.6 3.3 6.3 6.4 5.6 7.2 -5.2 -8.2 -7.8 – – –
Nucleus 1.3 -0.8 -3.8 -2.9 -2.3 -1.8 -2.5 -2.5 -2.4 -2.2 -4.9 -4.5 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus -1.1 0 -2.6 -1.9 -1.3 -1.4 -0.9 -0.4 -1.1 -1.6 0 -2.0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole -0.5 1.4 2.5 1.6 -0.2 -1.6 -1.6 -1.1 -2.3 2.2 3.0 2.7 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6956 9.4865 8.6714 8.6427 8.0477 8.4758 3.5353 5.2583 5.0956 4.8606 4.7548 5.1049 5.8945 6.9429 6.6272 6.7528 6.5481 6.9507
Actin 0.0119 0.0001 0.0197 0.0004 0.0009 0.0002 0.0144 0.0104 0.004 0.0076 0.0027 0.0006 0.0071 0 0.0171 0.0004 0.0044 0
Bud 0.0002 0.0001 0.0009 0.0001 0.0002 0.0001 0.001 0.0059 0.0006 0.0006 0.0002 0.0007 0.0002 0 0.0002 0.0003 0.0001 0.0001
Bud Neck 0.0005 0.0008 0.0027 0.0003 0.001 0.0034 0.0031 0.0006 0.0007 0.0262 0.0019 0.0039 0.0004 0.0001 0.0004 0.0008 0.0009 0.0021
Bud Periphery 0.0003 0 0.0007 0 0.0005 0.0001 0.0025 0.0038 0.0009 0.0007 0.0002 0.0014 0.0001 0 0.0001 0.0001 0 0.0001
Bud Site 0.0007 0.0007 0.0061 0.0001 0.0008 0.0002 0.0036 0.0141 0.0029 0.0165 0.0003 0.0014 0.0016 0.0001 0.0007 0.0003 0.0002 0.0002
Cell Periphery 0.0001 0 0.0004 0 0.0002 0 0.0007 0.0002 0.0002 0.0009 0 0.0001 0.0001 0 0 0 0 0
Cytoplasm 0.0074 0.0064 0.0146 0.0056 0.0021 0.0021 0.0152 0.0098 0.0043 0.0111 0.0046 0.0029 0.0147 0.0069 0.002 0.0049 0.0069 0.0014
Cytoplasmic Foci 0.0093 0.0003 0.0066 0.0005 0.0163 0.0001 0.068 0.0262 0.0032 0.0027 0.0024 0.0005 0.0293 0 0.0068 0.0001 0.0266 0
Eisosomes 0.0001 0 0.0004 0 0 0 0.0001 0 0.0001 0.0001 0 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0022 0.0003 0.0032 0.0004 0.0006 0.0007 0.0049 0.0021 0.0003 0.0002 0.0001 0.0011 0.0135 0 0.0007 0.0002 0.0006 0.0001
Endosome 0.0181 0.0009 0.0113 0.0014 0.0214 0.0018 0.0396 0.0119 0.0042 0.0019 0.0031 0.0052 0.056 0 0.0064 0.0001 0.0065 0
Golgi 0.0036 0.0001 0.0055 0.0001 0.0047 0.0001 0.0129 0.0064 0.0023 0.0034 0.0066 0.0019 0.0096 0 0.0106 0 0.0017 0
Lipid Particles 0.0115 0.0003 0.0072 0 0.0104 0.0001 0.0152 0.0115 0.0007 0.0024 0.028 0.0008 0.0121 0 0.002 0 0.006 0
Mitochondria 0.0013 0.0002 0.0096 0.0007 0.0025 0.0003 0.0149 0.0074 0.0251 0.0173 0.0139 0.0032 0.0017 0.0001 0.0022 0.0005 0.0003 0.0001
None 0.0141 0.0003 0.0034 0.0001 0.0006 0.0001 0.0055 0.001 0.0006 0.0073 0.0001 0.0002 0.001 0 0.0002 0.0002 0.0022 0
Nuclear Periphery 0.0298 0.0058 0.0065 0.0073 0.0067 0.0079 0.0179 0.0043 0.0026 0.0044 0.0056 0.0053 0.0165 0.0025 0.0033 0.0061 0.0089 0.0022
Nucleolus 0.0053 0.0077 0.0041 0.0024 0.0109 0.0114 0.006 0.0029 0.0028 0.0023 0.006 0.0084 0.0031 0.0019 0.006 0.002 0.0042 0.0091
Nucleus 0.857 0.9638 0.8859 0.9788 0.8883 0.9624 0.7313 0.8636 0.9296 0.8871 0.9032 0.9579 0.7896 0.9878 0.9222 0.9804 0.8696 0.9829
Peroxisomes 0.0064 0 0.0032 0.0001 0.0137 0 0.0165 0.0055 0.0011 0.0046 0.0094 0.0005 0.0316 0 0.0165 0 0.0037 0
Punctate Nuclear 0.0177 0.0116 0.0057 0.0013 0.0056 0.0074 0.0189 0.0083 0.0113 0.0013 0.0112 0.0019 0.0037 0.0005 0.002 0.0031 0.0567 0.0015
Vacuole 0.0013 0.0006 0.0017 0.0002 0.0098 0.0011 0.006 0.0033 0.0022 0.0012 0.0002 0.0009 0.0066 0.0001 0.0003 0.0002 0.0004 0.0002
Vacuole Periphery 0.0012 0.0001 0.0006 0.0002 0.0027 0.0003 0.0016 0.0008 0.0004 0.0002 0.0003 0.0011 0.0014 0 0.0002 0.0001 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.6535 30.1184 11.6402 16.2066 16.8361 13.3289 25.5358 28.6892 20.3399 28.1744
Translational Efficiency 1.3938 1.0683 1.1993 0.8707 1.3704 2.192 1.2747 0.8322 1.1123 1.0673

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Nam8

Nam8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nam8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available