Standard name
Human Ortholog
Description Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.05 0 0.21 0.11 0.12 0.05 0.05 0 0.19 0.19 0.15 0.15 0.19 0.17
Bud 0.12 0.14 0.11 0.13 0.21 0 0.06 0 0 0.06 0.16 0.2 0.3 0.18 0.24 0.15
Bud Neck 0 0 0 0 0.05 0.05 0.12 0.14 0.08 0.05 0.26 0.26 0.2 0.14 0.09 0.12
Bud Site 0 0.05 0 0.09 0.05 0.05 0 0.1 0.08 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.15 0.11 0.08 0.09 0.16 0.26 0.26 0.07 0.09 0.2 0 0.06 0.06 0.15 0.1 0.15
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Endosome 0.17 0.1 0.11 0 0.11 0.05 0 0 0.05 0 0 0 0 0 0.08 0.07
Golgi 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.28 0.32 0.29 0.52 0.05 0 0.06 0.29 0.16 0.14 0 0 0 0.06 0.07 0.06
Nucleus 0.08 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleolus 0.35 0.29 0.42 0.13 0.05 0.11 0.26 0.48 0.48 0.47 0 0.05 0 0.05 0 0.07
Peroxisomes 0.12 0.1 0.13 0.22 0.26 0.05 0.12 0.05 0.07 0 0.1 0 0.07 0 0 0
SpindlePole 0.07 0.06 0.11 0.13 0.21 0.16 0.09 0.05 0 0 0.12 0.05 0.05 0.08 0 0.06
Vac/Vac Membrane 0 0 0 0 0 0.21 0.06 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 2 0 4 2 4 3 6 4 9 13 8 17 9 19
Bud 5 11 4 3 4 0 2 2 1 6 7 13 16 20 12 17
Bud Neck 0 2 1 1 1 1 4 8 9 5 12 18 10 16 4 14
Bud Site 0 4 0 2 1 1 0 6 9 2
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 6 9 3 2 3 5 9 4 10 20 1 3 3 18 5 16
Endoplasmic Reticulum 0 1 0 0 0 1 0 0 3 4 0 0 1 3 0 2
Endosome 7 8 4 1 2 1 0 2 6 2 1 2 1 4 4 7
Golgi 0 0 0 0 0 0 0 0 4 5 1 0 0 1 0 2
Mitochondria 11 25 11 12 1 0 2 17 18 14 0 2 1 7 3 6
Nucleus 3 2 1 0 1 0 2 1 3 3 0 0 0 2 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Nucleolus 14 23 16 3 1 2 9 28 54 48 0 3 0 5 1 7
Peroxisomes 5 8 5 5 5 1 4 3 8 3 4 2 4 1 2 2
SpindlePole 3 5 4 3 4 3 3 3 4 4 5 3 3 9 1 7
Vac/Vac Membrane 1 0 1 1 0 4 2 3 4 4 1 1 0 2 1 3
Unique Cell Count 40 79 38 23 19 19 34 58 113 102 49 70 55 117 51 116
Labelled Cell Count 56 102 52 33 27 21 41 81 139 124 49 70 55 117 51 116


Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.8 12.6 12.6 10.6 16.4 18.1 15.1 28.2 28.1 25.7 16.7 15.9 16.7
Std Deviation (1e-4) 8.2 7.1 8.1 5.6 6.3 9.0 6.6 10.1 19.1 14.6 9.7 5.0 6.7
Intensity Change (Log2)

RAP220RAP300RAP380RAP700010203040HU80HU120HU160010203040rpd3Δ_1rpd3Δ_2rpd3Δ_3010203040AF100AF140AF180010203040
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 74.8224 74.1394 59.2005 51.9953 49.674 54.9595 73.74 65.7178 58.274 64.2699
Translational Efficiency 1.7768 1.732 1.398 1.5258 1.9523 1.9034 1.6044 1.2857 1.3784 1.3659

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
270 131 2014 324 20 1095 1633 162 290 1226 3647 486

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1173.49 1349.75 1511.17 1829.19 1241.26 1157.13 1571.66 1909.28 1178.16 1177.71 1538.26 1855.89
Standard Deviation 314.05 345.43 425.97 535.88 368.07 452.61 460.80 441.31 318.53 446.38 442.93 507.73
Intensity Change Log 2 0.201887 0.364860 0.640399 -0.101254 0.340484 0.621223 0.054019 0.352382 0.630574

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.019149 0.026050 0.006874 0.013128 0.007231 0.024344 0.003933 0.011559 0.018327 0.024526 0.005558 0.012605
Bud Neck 0.114231 0.142979 0.192016 0.158786 0.091476 0.114646 0.207688 0.136895 0.112662 0.117674 0.199033 0.151489
Bud Site 0.467418 0.442499 0.621978 0.623720 0.415695 0.514804 0.620753 0.642354 0.463851 0.507079 0.621429 0.629931
Cell Periphery 0.005738 0.008888 0.007797 0.007471 0.020246 0.008687 0.009959 0.006893 0.006738 0.008709 0.008765 0.007278
Cytoplasm 0.058614 0.052315 0.041418 0.024636 0.026628 0.059522 0.036352 0.027561 0.056408 0.058752 0.039150 0.025611
Cytoplasmic Foci 0.161119 0.091995 0.061712 0.072345 0.107309 0.116961 0.052322 0.082738 0.157408 0.114293 0.057507 0.075810
Eisosomes 0.007158 0.007921 0.007260 0.005162 0.008237 0.007266 0.007897 0.003090 0.007232 0.007336 0.007545 0.004471
Endoplasmic Reticulum 0.000552 0.000719 0.000273 0.001794 0.002845 0.001364 0.000289 0.003135 0.000710 0.001295 0.000280 0.002241
Endosome 0.004705 0.007530 0.001948 0.007801 0.012078 0.010101 0.002699 0.006707 0.005214 0.009827 0.002285 0.007436
Golgi 0.003089 0.004220 0.000936 0.006469 0.003051 0.004639 0.001575 0.010861 0.003086 0.004594 0.001222 0.007933
Lipid Particles 0.001649 0.004025 0.000193 0.003048 0.004047 0.002489 0.000250 0.000690 0.001814 0.002653 0.000219 0.002262
Mitochondria 0.032782 0.067703 0.016379 0.022355 0.061549 0.036609 0.016014 0.015136 0.034766 0.039932 0.016216 0.019948
Mitotic Spindle 0.005656 0.006500 0.004128 0.007183 0.001446 0.008757 0.004132 0.007312 0.005366 0.008516 0.004130 0.007226
None 0.002271 0.003519 0.001316 0.000358 0.008669 0.009223 0.000985 0.000268 0.002712 0.008613 0.001168 0.000328
Nuclear Periphery 0.000086 0.000130 0.000051 0.000197 0.000078 0.000131 0.000052 0.000108 0.000085 0.000131 0.000051 0.000167
Nuclear Periphery Foci 0.000831 0.002122 0.000271 0.000662 0.001365 0.000868 0.000309 0.000304 0.000868 0.001002 0.000288 0.000543
Nucleolus 0.005661 0.002550 0.001815 0.002603 0.010924 0.004339 0.002308 0.002207 0.006024 0.004148 0.002035 0.002471
Nucleus 0.007426 0.002693 0.003655 0.004634 0.005354 0.009245 0.003734 0.004517 0.007283 0.008545 0.003691 0.004595
Peroxisomes 0.087887 0.114475 0.017611 0.014578 0.197108 0.046233 0.014735 0.017285 0.095419 0.053525 0.016323 0.015480
Vacuole 0.013000 0.010136 0.011052 0.016939 0.012913 0.015687 0.012478 0.017353 0.012994 0.015094 0.011690 0.017077
Vacuole Periphery 0.000979 0.001030 0.001318 0.006131 0.001750 0.004084 0.001536 0.003027 0.001033 0.003758 0.001415 0.005096

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.71 2.47 1.04 1.41 -1.75 -4.32 1.20 -1.02 2.79 -2.19 -1.18 2.79 1.11 3.21 -2.70
Bud Neck -1.48 -7.10 -2.84 -0.54 3.29 -0.77 -3.95 -1.31 -1.30 4.94 -0.47 -8.56 -2.80 -3.13 5.69
Bud Site 0.69 -7.54 -5.87 -5.18 0.16 -1.09 -2.31 -2.50 -5.30 -0.99 -1.97 -7.99 -6.96 -7.33 -0.42
Cell Periphery -1.07 -1.69 -0.52 0.35 0.13 0.66 0.59 0.75 0.84 1.49 -1.14 -1.26 -0.22 0.61 0.68
Cytoplasm 0.51 2.08 3.34 1.74 2.78 -3.62 -1.39 -0.45 4.18 1.23 -0.24 2.20 3.35 5.64 2.88
Cytoplasmic Foci 3.44 6.75 5.05 0.92 -1.46 -0.36 1.65 0.69 2.62 -2.41 2.89 7.26 5.18 4.00 -2.70
Eisosomes -0.25 -0.03 0.86 1.07 1.30 0.37 0.16 1.82 3.61 4.08 -0.04 -0.14 1.36 2.19 2.63
Endoplasmic Reticulum -0.48 2.04 -1.38 -1.12 -1.72 0.89 1.56 -0.05 -0.62 -1.03 -1.57 2.51 -1.37 -0.80 -1.80
Endosome -0.97 2.36 -1.16 -0.00 -2.50 0.19 0.89 0.52 1.31 -1.53 -2.73 2.27 -0.96 1.28 -2.88
Golgi -0.84 2.67 -1.34 -0.80 -2.36 -0.71 0.71 -1.17 -0.97 -1.49 -1.47 2.41 -1.77 -1.19 -2.58
Lipid Particles -1.08 2.78 -0.77 0.47 -1.68 0.53 1.32 1.16 2.16 -1.21 -0.99 3.04 -0.32 0.37 -1.80
Mitochondria -2.07 2.69 1.36 2.74 -1.01 0.83 1.52 1.53 3.01 0.19 -0.71 3.10 2.12 3.55 -0.80
Mitotic Spindle -0.40 0.93 -0.68 -0.33 -1.33 -4.29 -3.51 -1.62 0.47 -0.81 -1.69 0.79 -0.87 0.53 -1.55
None -0.48 1.26 2.63 1.23 2.71 -0.07 1.02 1.12 4.64 5.20 -3.01 1.83 2.83 4.74 4.11
Nuclear Periphery -1.07 2.41 -2.23 -0.91 -2.88 -1.83 0.77 -0.83 0.51 -1.56 -2.37 2.49 -2.31 -1.05 -3.22
Nuclear Periphery Foci -1.16 2.29 0.53 1.31 -1.60 0.98 2.23 2.25 4.07 0.28 -0.52 2.52 1.21 2.08 -1.53
Nucleolus 1.23 1.60 1.25 0.06 -0.99 1.01 1.33 1.34 1.62 0.17 0.77 1.75 1.54 1.99 -0.56
Nucleus 2.84 2.14 0.07 -2.13 -1.54 -1.07 0.25 -0.20 0.85 -0.56 -0.73 2.20 0.17 0.81 -1.53
Peroxisomes -1.31 7.12 7.19 5.54 1.03 2.24 2.70 2.67 5.29 -0.50 3.81 7.63 7.56 8.62 0.44
Vacuole 0.78 0.47 -1.22 -2.07 -1.95 -0.57 0.02 -0.60 -0.28 -0.77 -0.82 0.33 -1.26 -0.71 -1.85
Vacuole Periphery -0.33 -1.16 -2.27 -2.20 -2.09 -1.34 0.13 -0.62 0.64 -0.98 -3.00 -1.33 -2.54 -0.70 -2.27
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Componentsactinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
Localization
Cell Percentages mitochondrion (16%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Cdc12

Cdc12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available