Standard name
Human Ortholog
Description Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0.05 0 0.06 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.9 0.96 0.92 0.85 0.74 0.74 0.77 0.84 0.85 0.87 0.88 0.81 0.86 0.86 0.51 0.51 0.54 0.17 0.29 0.27 0.13 0.19 0.17
Endoplasmic Reticulum 0 0.09 0 0 0.08 0.12 0.08 0 0 0 0 0 0.14 0.06 0.05 0 0 0 0.19 0.34 0.34 0.07 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.08 0.12 0.2 0.21 0.19 0.19 0.15 0.17 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.16 0.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0.07 0.06 0.57 0.48 0.46 0.49 0.25 0.25 0.61 0.64 0.65
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 1 8 4 3 1 0 1 2 2 2 2 0 1 0 1 2 1 2 1 1
Bud 0 1 2 2 15 19 20 33 25 22 23 18 3 2 0 0 0 0 2 5 3 2 4 2
Bud Neck 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 3 8 1 1 0 0
Bud Site 0 0 0 0 0 2 1 1 0 2 3 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 1 6 11 8 1 3 5 1 5 0 0 2 0 0 1 0 1 0 0 0 0
Cytoplasm 179 327 204 200 240 275 341 450 374 411 349 440 218 237 282 121 81 82 42 116 59 23 57 47
Endoplasmic Reticulum 8 33 2 8 22 43 35 14 6 2 3 4 39 17 17 4 2 3 47 138 74 13 11 15
Endosome 2 0 3 1 1 4 1 1 3 0 0 1 2 8 8 2 4 3 7 12 4 12 11 6
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 3 1 0 1 5 3
Mitochondria 4 1 6 12 22 46 93 124 84 91 60 86 0 0 0 2 3 0 9 12 14 6 6 11
Nucleus 0 0 0 0 1 0 6 11 0 5 4 3 1 1 5 26 26 15 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 1 1 8 0 1 1 0 0 0 0 0 0 2 1 1 0 0 2 1
Nucleolus 0 0 0 0 0 0 1 3 1 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0
SpindlePole 0 0 1 0 0 1 0 0 0 2 0 2 1 1 2 0 1 0 1 1 0 1 2 2
Vac/Vac Membrane 25 6 1 2 4 7 9 20 16 29 23 20 11 19 21 134 77 69 120 101 54 108 200 182
Unique Cell Count 208 365 213 217 282 370 459 586 446 483 402 499 270 274 327 237 159 151 245 408 222 179 312 281
Labelled Cell Count 220 373 219 226 312 417 521 669 514 570 470 582 277 288 340 289 195 175 245 408 222 179 312 281


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.0 4.3 4.6 5.0 5.0 4.5 4.7 4.3 3.9 4.1 3.9 6.7 6.2 6.3 7.4 7.1 7.9 6.9 7.0 6.9
Std Deviation (1e-4) 0.9 1.0 0.9 1.3 1.7 1.2 1.3 1.4 1.1 1.0 1.2 1.1 1.1 1.0 1.0 1.9 1.5 1.7 1.6 1.9 1.6
Intensity Change (Log2) – – – 0.09 0.22 0.22 0.09 0.14 0.0 -0.14 -0.06 -0.14 0.66 0.54 0.56 0.79 0.74 0.89 0.69 0.72 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2.6 2.6 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -3.9 -6.5 -6.6 -6.2 -4.4 -4.0 -3.5 -3.2 -4.9 -3.5 -3.6 -10.6 -10.1 -9.5
Endoplasmic Reticulum 0 3.5 4.7 0 0 0 0 0 0 5.3 3.0 2.6 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.4 3.9 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 5.0 6.1 4.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.3 2.4 3.3 3.2 2.6 2.5 3.6 3.4 13.0 11.2 10.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8639 2.7856 3.0581 2.6709 2.4365 2.2097 3.1263 4.2308 4.1835 3.8877 3.4718 3.6904 0.1362 1.8779 1.5602 1.1444 1.2969 0.8587
Actin 0.015 0.0002 0.0072 0.0085 0.009 0.0007 0.0081 0.0002 0.0025 0.0001 0.0177 0.0015 0.0004 0.0001 0.0001 0 0 0
Bud 0.0004 0.0006 0.0057 0.0006 0.0022 0.0006 0.0007 0.0006 0.0004 0.0003 0.0017 0.0003 0.0002 0.0033 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0009 0.0004 0.0065 0.0011 0.0008 0.0014 0.0067 0.0003 0.0008 0.0006 0.0012 0.0031 0.0002 0.0003 0.0034 0.0039 0.0005 0.0006
Bud Periphery 0.0005 0.0006 0.0029 0.0006 0.0029 0.0008 0.0012 0.0008 0.0004 0.0005 0.0015 0.0004 0.0003 0.0034 0.0002 0.0002 0.0002 0.0001
Bud Site 0.0015 0.0037 0.0056 0.0016 0.0054 0.0003 0.0074 0.0029 0.0056 0.0004 0.0028 0.0006 0.0007 0.0104 0.0013 0.0005 0.0002 0.0001
Cell Periphery 0.0004 0.0007 0.0005 0.0003 0.0002 0.0002 0.001 0.0008 0.001 0.0005 0.0006 0.0003 0.0006 0.0006 0.0005 0.0003 0.0001 0.0002
Cytoplasm 0.3915 0.51 0.471 0.3422 0.4782 0.5086 0.3101 0.5347 0.4112 0.2511 0.3426 0.4588 0.3618 0.4327 0.4635 0.3061 0.5903 0.3848
Cytoplasmic Foci 0.0451 0.0444 0.0452 0.0308 0.0327 0.046 0.0569 0.0333 0.0369 0.0233 0.0522 0.0481 0.0303 0.032 0.0302 0.0543 0.0187 0.0365
Eisosomes 0.0001 0 0.0001 0.0001 0.0002 0 0.0004 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.0687 0.1122 0.1373 0.0264 0.0501 0.1012 0.1059 0.1261 0.1259 0.0828 0.0838 0.1071 0.143 0.1467 0.0951 0.0856 0.0847
Endosome 0.159 0.1241 0.1532 0.2337 0.1545 0.1378 0.1649 0.103 0.1513 0.2338 0.1902 0.156 0.1817 0.1226 0.1572 0.1959 0.1319 0.193
Golgi 0.0081 0.0031 0.0061 0.0061 0.0111 0.0023 0.0098 0.0023 0.0097 0.0055 0.0272 0.0064 0.0053 0.0016 0.0043 0.0013 0.0006 0.0014
Lipid Particles 0.0186 0.0035 0.007 0.0074 0.0168 0.0087 0.0223 0.0041 0.0098 0.0017 0.0267 0.0163 0.0116 0.0045 0.0075 0.003 0.0006 0.002
Mitochondria 0.0048 0.002 0.0063 0.0035 0.0524 0.0039 0.0093 0.0015 0.0078 0.0045 0.0119 0.0041 0.0065 0.001 0.0107 0.0005 0.0004 0.0011
None 0.1723 0.1218 0.0793 0.1136 0.0492 0.1114 0.1717 0.0932 0.1109 0.0051 0.0449 0.0834 0.1499 0.0874 0.0667 0.072 0.0147 0.0628
Nuclear Periphery 0.0193 0.0156 0.015 0.0364 0.0209 0.0133 0.0129 0.0136 0.0132 0.0143 0.0187 0.0125 0.0161 0.0183 0.0142 0.0166 0.0118 0.0149
Nucleolus 0.0005 0.0007 0.0005 0.0004 0.001 0.0005 0.0015 0.0003 0.0005 0.0001 0.0007 0.0012 0.0004 0.001 0.0011 0.0005 0.0001 0.0003
Nucleus 0.0073 0.0101 0.006 0.005 0.0135 0.0103 0.0042 0.005 0.0039 0.0037 0.0065 0.0064 0.0043 0.0055 0.0038 0.0066 0.0053 0.009
Peroxisomes 0.0146 0.0011 0.0021 0.0016 0.0061 0.0131 0.0062 0.0003 0.0077 0.0001 0.0242 0.0016 0.0012 0.0004 0.001 0.0001 0.0001 0.0001
Punctate Nuclear 0.0041 0.0017 0.0036 0.0037 0.0215 0.0039 0.0071 0.0007 0.001 0.0003 0.0052 0.0022 0.001 0.0022 0.0007 0.0013 0.0004 0.0006
Vacuole 0.06 0.0813 0.0574 0.0555 0.0827 0.0767 0.0904 0.0902 0.0919 0.28 0.1288 0.1029 0.1119 0.1228 0.08 0.2263 0.1266 0.1877
Vacuole Periphery 0.0057 0.0056 0.0066 0.0099 0.0123 0.0095 0.0059 0.0062 0.0075 0.0483 0.0116 0.0102 0.0085 0.0068 0.0066 0.0154 0.0119 0.02

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 99.2891 44.7651 13.7452 38.008 97.3891 84.5648 39.7993 38.976 39.0407 78.8593
Translational Efficiency 0.6373 0.5466 0.8284 0.5747 0.4852 0.8091 0.6324 0.3864 0.6333 0.6277

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport
Localization
Cell Percentages ER (12%), cytoplasm (11%), mixed (34%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Erp5

Erp5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erp5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available