Standard name
Human Ortholog
Description E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.1 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 – 0 0 0.07 0 0.1 0.09 0.19 0.08 0.06 0.13 0 0.08 0 0.11 0.06 – 0 0 0 0 0 0
Cytoplasm 0.9 – 0.78 0.8 0.76 0.79 0.7 0.67 0.67 0.58 0.63 0.59 0.9 0.96 0.87 0.8 0.59 – 0.86 0.89 0.72 0.76 0.81 0.87
Endoplasmic Reticulum 0 – 0.06 0 0 0 0 0 0 0 0.08 0 0.06 0 0 0.05 0 – 0 0 0.06 0 0 0
Endosome 0 – 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0 0 0.18 – 0 0 0 0.12 0.08 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.12 – 0 0 0.06 0 0 0
Mitochondria 0.1 – 0.09 0.27 0.26 0.15 0.27 0.2 0.22 0.39 0.33 0.32 0 0 0 0.05 0.06 – 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 – 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 – 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0.05 0.05 0.08 0 0 0 0 0 0.09 0.12 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 – 3 0 1 1 4 0 2 2 1 1 0 0 0 0 0 – 0 0 1 0 0 2
Bud 1 – 0 0 2 0 2 1 1 5 0 1 0 0 0 0 0 – 0 1 0 0 0 1
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 1 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 2 – 3 1 8 2 13 12 20 10 3 12 1 2 0 5 1 – 0 0 2 0 0 0
Cytoplasm 121 – 69 66 84 37 87 92 71 75 32 54 28 24 20 35 10 – 122 43 78 5 38 108
Endoplasmic Reticulum 4 – 5 2 1 2 1 2 3 1 4 2 2 1 0 2 0 – 5 0 6 0 0 0
Endosome 2 – 3 3 4 2 7 10 2 5 0 1 1 0 0 0 3 – 0 0 2 0 3 2
Golgi 1 – 4 2 3 1 4 2 0 0 0 1 1 0 2 2 2 – 3 0 6 0 1 2
Mitochondria 14 – 8 22 29 7 34 27 23 50 17 29 0 0 1 2 1 – 0 0 3 0 0 0
Nucleus 0 – 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 1 – 4 1 3 2 2 0 1 3 0 2 0 0 1 0 3 – 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 – 1 0 0 0 0 0 1 1 0 0 0 0 0 1 2 – 0 0 2 0 0 0
Vac/Vac Membrane 3 – 3 0 2 1 4 7 5 10 2 3 0 0 1 4 2 – 2 0 0 0 1 1
Unique Cell Count 135 89 82 110 47 124 138 106 129 51 91 31 25 23 44 17 143 49 109 7 47 124
Labelled Cell Count 152 103 97 137 55 159 154 129 163 59 106 33 27 25 51 24 143 49 109 7 47 124


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 – 10.0 9.0 9.4 9.5 9.0 9.2 9.1 8.3 7.9 8.5 12.4 11.7 11.9 14.1 14.4 – – 11.2 11.7
Std Deviation (1e-4) 1.3 – 1.7 1.9 2.5 2.5 2.2 2.5 2.3 2.1 1.8 2.0 2.0 2.0 2.7 5.3 3.8 – – 1.8 1.9
Intensity Change (Log2) – – – -0.15 -0.08 -0.08 -0.15 -0.12 -0.14 -0.27 -0.34 -0.23 0.3 0.23 0.25 0.5 0.53 – – 0.17 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 1.9 1.6 3.3 1.3 0 2.4 0 0 0 0 0 –
Cytoplasm 0.5 -0.2 0.2 -1.2 -1.8 -1.6 -3.0 -1.9 -2.6 1.6 2.1 1.0 0.3 -1.6 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 0 0 1.2 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Mitochondria 3.1 3.1 1.0 3.3 2.2 2.4 4.9 3.6 3.8 0 0 0 0 0 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Vacuole 0 0 0 0 0 0 1.3 0 0 0 0 0 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3917 7.4091 6.6342 4.9326 5.9404 6.0501 3.3219 5.9464 5.4467 4.6156 4.468 4.935 2.9391 6.1932 4.9592 4.2304 3.658 4.5916
Actin 0.0529 0.0009 0.0102 0.0003 0.006 0.0115 0.0469 0.0006 0.0069 0.0008 0.0008 0.011 0.0335 0.002 0.0454 0.0065 0.0139 0.0026
Bud 0.0042 0.0005 0.001 0.0008 0.0023 0.0006 0.001 0.001 0.0009 0.001 0.0005 0.0007 0.0009 0.0004 0.0018 0.0017 0.0011 0.0003
Bud Neck 0.0069 0.0007 0.0014 0.0006 0.0011 0.0013 0.0034 0.0003 0.0004 0.0007 0.0004 0.002 0.0025 0.0006 0.0009 0.0026 0.0012 0.0008
Bud Periphery 0.0095 0.0003 0.0006 0.0004 0.0017 0.0004 0.0009 0.001 0.0006 0.0003 0.0001 0.0006 0.0009 0.0001 0.0014 0.001 0.001 0.0003
Bud Site 0.0104 0.0069 0.0047 0.0006 0.0018 0.0005 0.0062 0.0024 0.0011 0.0007 0.0004 0.0014 0.0025 0.0082 0.0091 0.0559 0.0025 0.0002
Cell Periphery 0.0045 0.0018 0.0008 0.0006 0.0002 0.0006 0.0003 0.0008 0.0001 0.0001 0 0.0001 0.0004 0.0003 0.0003 0.0002 0.0001 0
Cytoplasm 0.5594 0.8891 0.8268 0.7893 0.7847 0.807 0.6679 0.9151 0.8872 0.8095 0.7676 0.7225 0.5814 0.9263 0.7791 0.6784 0.7845 0.8443
Cytoplasmic Foci 0.0456 0.0167 0.0361 0.0283 0.0264 0.0311 0.0508 0.0142 0.0172 0.0271 0.0623 0.0296 0.0403 0.0104 0.0201 0.0457 0.0327 0.0228
Eisosomes 0.0004 0 0.0001 0 0 0.0003 0.0003 0 0 0 0 0.0001 0.0002 0 0.0005 0.0001 0.0001 0
Endoplasmic Reticulum 0.048 0.0084 0.0067 0.0097 0.0072 0.0166 0.0242 0.0031 0.0062 0.006 0.0047 0.0336 0.0228 0.0077 0.0112 0.0296 0.0162 0.0166
Endosome 0.1574 0.0438 0.0719 0.1403 0.1074 0.0709 0.0737 0.0295 0.0459 0.1178 0.0956 0.1302 0.1211 0.0304 0.064 0.1222 0.06 0.0587
Golgi 0.0309 0.0034 0.016 0.0024 0.03 0.0274 0.018 0.0018 0.0085 0.0048 0.017 0.033 0.0171 0.0034 0.0049 0.0081 0.0185 0.0043
Lipid Particles 0.0032 0.0003 0.0017 0 0.0007 0.0008 0.0185 0.0012 0.0009 0.0006 0.027 0.0046 0.0272 0.0002 0.0028 0.0054 0.031 0.0066
Mitochondria 0.0105 0.0006 0.0063 0.0004 0.0133 0.007 0.0083 0.0008 0.0024 0.0026 0.0019 0.009 0.0134 0.0007 0.0088 0.0024 0.0052 0.0036
None 0.0197 0.0078 0.0018 0.0008 0.0007 0.0163 0.0167 0.0032 0.0014 0.0015 0.0015 0.0008 0.0327 0.001 0.0035 0.0015 0.0007 0.0013
Nuclear Periphery 0.005 0.0012 0.001 0.0012 0.0021 0.0006 0.0091 0.0006 0.004 0.0035 0.0011 0.0023 0.0317 0.0007 0.002 0.0037 0.0021 0.0175
Nucleolus 0.0005 0.0001 0.0001 0 0.0001 0.0001 0.0008 0.0052 0.0003 0.0001 0.0001 0.0001 0.0017 0 0.0001 0.0008 0.0002 0.0006
Nucleus 0.0026 0.0012 0.0009 0.001 0.0015 0.0006 0.0046 0.0088 0.0019 0.0031 0.0011 0.0012 0.0197 0.0008 0.0014 0.0021 0.0021 0.0037
Peroxisomes 0.0011 0.0001 0.0046 0.0001 0.0008 0.0011 0.0229 0.0001 0.0006 0.0004 0.0081 0.0026 0.0114 0.0001 0.0043 0.0041 0.0093 0.0035
Punctate Nuclear 0.0014 0.0002 0.0004 0.0001 0.0003 0.0002 0.0077 0.0005 0.0015 0.0014 0.0012 0.0013 0.0086 0.0001 0.0008 0.0031 0.001 0.0048
Vacuole 0.0214 0.0143 0.0054 0.0187 0.0076 0.0035 0.0148 0.0082 0.0093 0.0139 0.0062 0.0088 0.0235 0.0053 0.0317 0.0189 0.0127 0.0041
Vacuole Periphery 0.0046 0.0017 0.0017 0.0044 0.004 0.0015 0.003 0.0017 0.0027 0.0043 0.0025 0.0048 0.0064 0.0013 0.0061 0.0059 0.0038 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.6803 22.3668 24.3425 21.692 20.5454 19.1718 21.8612 27.2202 26.2927 26.826
Translational Efficiency 1.5314 1.0633 1.1521 1.297 1.2355 1.3545 1.5684 1.0838 1.2404 1.0084

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
50 148 1820 940 1443 1289 1675 72 1493 1437 3495 1012

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1070.91 1042.68 1284.93 1341.02 918.59 959.29 1332.00 1489.17 923.69 967.88 1307.49 1351.56
Standard Deviation 296.91 168.57 187.18 281.71 140.87 135.34 197.21 257.45 151.27 141.42 193.49 282.63
Intensity Change Log 2 — -0.038541 0.262853 0.324494 — 0.062546 0.536101 0.697015 — 0.009014 0.395469 0.508493

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006324 0.000762 0.004701 0.004848 0.000487 0.000694 0.002837 0.005363 0.000683 0.000701 0.003808 0.004884
Bud Neck 0.003343 0.002357 0.003911 0.005522 0.004530 0.004835 0.002404 0.010558 0.004491 0.004579 0.003189 0.005881
Bud Site 0.108208* 0.093714 0.060051 0.087094 0.041580 0.048365 0.063666 0.136434* 0.043812 0.053036 0.061784 0.090604
Cell Periphery 0.002851 0.000443 0.000300 0.003166 0.000372 0.000243 0.000378 0.003911 0.000455 0.000264 0.000337 0.003219
Cytoplasm 0.115990* 0.495086* 0.516628* 0.725470* 0.468257* 0.424891* 0.615211* 0.550148* 0.456460* 0.432121* 0.563874* 0.712996*
Cytoplasmic Foci 0.243151* 0.274797* 0.155047 0.054873 0.236946* 0.256561* 0.146873 0.110356 0.237154* 0.258439* 0.151130 0.058820
Eisosomes 0.000136 0.000023 0.000019 0.000019 0.000054 0.000049 0.000017 0.000041 0.000057 0.000046 0.000018 0.000020
Endoplasmic Reticulum 0.039753 0.001806 0.001591 0.001013 0.007586 0.004040 0.001725 0.001040 0.008663 0.003810 0.001655 0.001015
Endosome 0.048737 0.038778 0.038219 0.006834 0.033401 0.049705 0.025936 0.019842 0.033915 0.048579 0.032332 0.007760
Golgi 0.087891 0.029972 0.026664 0.011460 0.021707 0.024977 0.028150 0.016391 0.023924 0.025491 0.027376 0.011810
Lipid Particles 0.023834 0.001564 0.008585 0.014825 0.003276 0.002111 0.006947 0.023891 0.003964 0.002054 0.007800 0.015470
Mitochondria 0.050134 0.000604 0.000938 0.000346 0.004818 0.005973 0.000882 0.000398 0.006336 0.005420 0.000911 0.000349
Mitotic Spindle 0.041864 0.001954 0.043374 0.005147 0.003210 0.008666 0.013448 0.003288 0.004505 0.007975 0.029031 0.005015
None 0.002540 0.000081 0.000223 0.000228 0.000589 0.000464 0.000142 0.000109 0.000654 0.000425 0.000184 0.000220
Nuclear Periphery 0.002709 0.000015 0.000280 0.000051 0.000189 0.000159 0.000057 0.000056 0.000274 0.000144 0.000173 0.000052
Nuclear Periphery Foci 0.032849 0.000494 0.002929 0.002591 0.000335 0.000304 0.001710 0.004433 0.001424 0.000324 0.002345 0.002722
Nucleolus 0.011898 0.000043 0.000147 0.000082 0.000212 0.000295 0.000075 0.000529 0.000604 0.000269 0.000113 0.000113
Nucleus 0.084104 0.001194 0.005551 0.005105 0.009676 0.004237 0.002693 0.003001 0.012168 0.003923 0.004181 0.004955
Peroxisomes 0.015787 0.003389 0.004371 0.003178 0.001475 0.003180 0.003267 0.004307 0.001954 0.003201 0.003842 0.003258
Vacuole 0.046624 0.043080 0.115800 0.065544 0.131515 0.135564 0.074151 0.103933 0.128673 0.126039 0.095839 0.068275
Vacuole Periphery 0.031275 0.009843 0.010674 0.002606 0.029782 0.024688 0.009431 0.001972 0.029832 0.023159 0.010079 0.002561

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.18 0.93 0.67 -4.94 -0.69 -1.56 -4.94 -2.91 -2.77 -1.35 -0.01 -6.45 -5.36 -5.37 -1.56
Bud Neck 1.29 -0.46 -1.61 -5.65 -2.64 -0.53 4.39 -1.34 -1.27 -1.86 -0.15 2.33 -2.32 -2.29 -4.60
Bud Site 0.54 2.29 0.93 0.76 -5.84 -2.25 -6.49 -4.66 -4.28 -3.52 -2.98 -5.44 -8.55 -6.55 -5.97
Cell Periphery 2.73 2.95 0.03 -4.25 -4.61 2.43 0.07 -2.52 -2.63 -2.53 3.19 2.34 -4.59 -4.98 -4.88
Cytoplasm -11.19 -16.37 -23.65 -8.13 -16.23 3.79 -13.36 -2.28 -3.49 1.88 2.18 -11.23 -20.03 -22.14 -12.68
Cytoplasmic Foci -0.90 3.10 5.53 12.23 15.60 -2.42 13.73 8.03 9.24 2.05 -2.73 15.77 26.68 29.99 17.60
Eisosomes 3.62 3.81 3.80 1.06 -0.09 0.75 8.51 1.60 1.03 -2.09 1.62 9.33 8.42 4.83 -1.35
Endoplasmic Reticulum 3.36 3.40 3.44 1.45 1.55 2.84 5.48 6.20 4.45 2.43 3.88 6.43 6.91 4.46 2.23
Endosome 0.73 1.50 4.15 4.19 18.02 -6.26 4.30 3.11 5.97 1.58 -5.76 2.71 16.38 19.11 19.75
Golgi 2.97 3.55 4.13 4.32 5.22 -1.88 -2.56 1.83 2.47 2.76 -0.89 0.28 7.42 7.68 7.41
Lipid Particles 2.58 1.71 0.98 -9.75 -4.07 2.16 -5.88 -3.19 -3.37 -2.60 3.26 -6.62 -8.28 -9.86 -5.49
Mitochondria 2.25 2.24 2.26 1.01 2.83 -1.02 6.64 7.67 5.47 1.34 0.70 5.98 6.53 5.62 2.56
Mitotic Spindle 2.02 -0.48 1.80 -2.66 12.38 -2.87 -5.70 0.05 2.84 5.60 -1.87 -12.90 -0.89 1.00 11.71
None 3.41 3.20 3.18 -4.02 -0.28 0.45 2.18 2.28 1.80 0.57 0.89 2.34 2.08 1.07 -1.07
Nuclear Periphery 1.99 1.71 1.95 -6.62 5.92 0.42 2.98 2.80 1.48 -0.28 1.62 0.57 3.39 1.48 5.78
Nuclear Periphery Foci 2.31 2.10 2.14 -8.94 1.25 0.24 -6.64 -4.33 -4.37 -2.91 2.19 -2.20 -2.85 -11.20 -1.10
Nucleolus 2.23 2.21 2.22 -4.28 7.19 -0.61 3.28 -0.60 -0.38 -0.92 1.53 2.51 2.54 1.26 0.41
Nucleus 3.06 2.74 2.83 -9.16 2.92 4.40 5.65 3.33 -0.25 -1.27 5.41 3.64 3.16 -4.34 -0.66
Peroxisomes 1.56 1.51 1.61 0.75 1.73 -5.58 -4.20 -3.28 -0.84 -0.85 -3.06 -3.96 -1.99 0.44 1.10
Vacuole 0.39 -7.66 -2.48 -4.39 10.74 -0.49 7.87 0.72 0.94 -2.17 0.37 2.82 6.93 6.95 6.30
Vacuole Periphery 2.88 3.17 4.05 3.96 7.67 2.21 11.14 15.93 14.46 8.56 3.05 12.09 16.11 14.51 10.35
Standard name
Human Ortholog
Description E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
Localization
Cell Percentages cytoplasm (93%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Uba4

Uba4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Uba4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available