ORF
Human Ortholog
Description Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.95 0.98 0.97 0.94 0.85 0.77 0.82 0.72 0.75 0.64 0.7 0.99 0.98 0.99 0.89 0.89 0.91 0.82 0.86 0.84 0.85 0.87 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.22 0.32 0.23 0.4 0.33 0.55 0.32 0 0 0 0 0 0 0 0.05 0.08 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.05 0.09 0.07 0.05 0.05 0 0.08 0 0 0 0.1 0.12 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 0 0 0 0 0 0 1 1 0 0 0 0 0 1 12 0 3 2 4 3
Bud 2 0 1 0 0 1 9 11 16 13 15 9 0 0 0 0 0 0 8 1 4 0 10 17
Bud Neck 0 0 8 5 4 1 1 0 0 1 0 1 1 1 0 0 0 0 4 0 1 0 4 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 4 4 5 7 7 8 9 11 3 5 3 2 2 18 13 18 1 0 4 0 1 2
Cytoplasm 511 170 567 472 648 564 625 656 518 478 299 331 356 453 484 468 288 440 434 167 463 175 410 382
Endoplasmic Reticulum 2 0 1 0 0 0 0 0 3 0 1 2 1 0 0 18 13 16 4 0 4 1 3 3
Endosome 2 0 3 0 4 8 1 0 0 1 0 1 0 1 0 3 3 2 9 3 3 6 2 2
Golgi 2 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 2 0 2 2 1 0 0 1
Mitochondria 24 6 5 7 31 144 259 185 288 212 258 151 1 2 0 7 4 9 23 10 41 9 15 15
Nucleus 0 0 1 6 2 4 3 8 7 7 4 8 0 1 0 15 3 9 0 1 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 2 1 2 2 0 1 0 0 0 0 0 0 1 0 1 0 1 1
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 2 2 0 3 2 2 0 1 3 1 3 0 0 1 0 0 0 5 0 5 2 7 6
Vac/Vac Membrane 4 2 4 9 31 31 75 53 34 34 8 36 1 6 6 50 39 47 5 3 4 1 1 4
Unique Cell Count 534 179 578 485 688 666 815 803 715 641 469 475 360 462 488 524 324 486 527 196 554 207 471 458
Labelled Cell Count 547 183 597 504 729 762 985 923 878 763 590 549 364 469 493 579 365 542 527 196 554 207 471 458


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.4 5.2 4.8 5.3 4.4 4.4 4.9 4.3 4.6 4.0 4.6 5.5 5.5 5.5 7.0 7.8 7.3 5.4 5.3 6.0
Std Deviation (1e-4) 1.1 0.9 1.3 1.3 1.5 1.1 1.0 1.3 1.1 1.2 1.0 1.2 1.4 1.6 1.7 1.6 1.8 1.6 1.8 1.7 2.6
Intensity Change (Log2) – – – -0.1 0.03 -0.23 -0.23 -0.07 -0.27 -0.17 -0.37 -0.18 0.08 0.1 0.1 0.43 0.59 0.51 0.06 0.04 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.8 7.7 6.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3641 0.9763 0.6145 0.574 0.4204 0.7746 -0.4742 0.0252 -0.0134 -0.2738 -0.3955 0.021 -0.5227 -0.7657 -0.4503 -0.7841 -0.2209 -0.7045
Actin 0.0003 0.0005 0.0003 0.0003 0.0009 0.0004 0.0057 0.0008 0.0005 0.0005 0.0025 0.0049 0.0296 0.0007 0.0166 0.0003 0.0687 0.0049
Bud 0.0001 0.0002 0.0009 0.0002 0.0005 0.0006 0.0096 0.0061 0.0117 0.0067 0.0458 0.0046 0.0074 0.0045 0.0065 0.0099 0.0103 0.0075
Bud Neck 0.0032 0.0001 0.0001 0.0001 0.0038 0.0004 0.005 0.003 0.0015 0.0002 0.0002 0.001 0.0061 0.0002 0.0021 0.0007 0.0012 0.0014
Bud Periphery 0.0001 0.0002 0.0007 0.0003 0.0014 0.0002 0.003 0.0015 0.0041 0.0046 0.008 0.0021 0.0046 0.005 0.0022 0.003 0.0237 0.006
Bud Site 0.0006 0.0007 0.0014 0.0001 0.0011 0.0001 0.003 0.0084 0.0077 0.0079 0.0022 0.0005 0.0134 0.0174 0.0167 0.0035 0.0077 0.0008
Cell Periphery 0.0001 0.0001 0 0.0001 0.0003 0.0001 0.0006 0.0023 0.0003 0.0004 0.0004 0.0001 0.0005 0.0003 0.0004 0.0006 0.0005 0.0002
Cytoplasm 0.0318 0.0992 0.062 0.043 0.0652 0.0964 0.046 0.0605 0.0678 0.0819 0.1269 0.0943 0.081 0.1635 0.186 0.2305 0.1017 0.203
Cytoplasmic Foci 0.0033 0.0042 0.0043 0.0241 0.0016 0.006 0.0205 0.0069 0.0053 0.0078 0.0119 0.0097 0.0295 0.0152 0.0158 0.0092 0.0539 0.0202
Eisosomes 0.0002 0.0003 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0006 0.0001 0.0003 0 0.0006 0.0001
Endoplasmic Reticulum 0.0003 0.0011 0.0004 0.0012 0.0006 0.0007 0.0019 0.0004 0.0005 0.0005 0.0004 0.0006 0.0063 0.0012 0.0025 0.0009 0.0033 0.0015
Endosome 0.0004 0.0011 0.0004 0.0017 0.0005 0.0006 0.0042 0.0033 0.0011 0.0016 0.0007 0.0022 0.0163 0.002 0.0097 0.0014 0.006 0.0045
Golgi 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0012 0.0003 0.0002 0.0001 0.0005 0.0015 0.0031 0.0007 0.0031 0.0001 0.0035 0.002
Lipid Particles 0.0003 0.0002 0.0002 0.0035 0.0002 0.0002 0.0033 0.0003 0.0003 0.0001 0.0006 0.0008 0.0055 0.0004 0.0037 0.0002 0.0035 0.0016
Mitochondria 0.0004 0.0002 0.0003 0.0005 0.0006 0.0002 0.0024 0.0005 0.001 0.0004 0.0016 0.003 0.0071 0.001 0.0026 0.0004 0.0306 0.0014
None 0.9527 0.8814 0.9234 0.9074 0.9192 0.8877 0.8695 0.8796 0.8849 0.8624 0.7316 0.8594 0.7461 0.7715 0.7061 0.7232 0.6419 0.7163
Nuclear Periphery 0.0002 0.0005 0.0003 0.001 0.0003 0.0005 0.006 0.0005 0.0007 0.0015 0.0004 0.0007 0.017 0.0012 0.0026 0.001 0.0028 0.0089
Nucleolus 0.0019 0.0003 0.0003 0.0008 0.0008 0.0003 0.0009 0.001 0.001 0.0018 0.0007 0.0005 0.0013 0.001 0.001 0.0009 0.0033 0.0006
Nucleus 0.0017 0.0039 0.0022 0.0011 0.0013 0.0026 0.0082 0.0041 0.004 0.0112 0.009 0.0025 0.0056 0.0039 0.0088 0.0059 0.0061 0.0085
Peroxisomes 0.0001 0.0001 0.0003 0.0012 0.0002 0.0002 0.0028 0.002 0.0006 0.0004 0.0521 0.0014 0.0107 0.0019 0.0075 0.0001 0.0096 0.0046
Punctate Nuclear 0.0019 0.0052 0.002 0.0123 0.0006 0.0023 0.0043 0.0124 0.0061 0.0089 0.0033 0.0094 0.0043 0.0065 0.0036 0.0061 0.0178 0.004
Vacuole 0.0003 0.0004 0.0002 0.0008 0.0004 0.0004 0.0011 0.0057 0.0006 0.0008 0.0009 0.0005 0.0025 0.0015 0.0019 0.0019 0.0022 0.0013
Vacuole Periphery 0 0.0001 0 0.0002 0.0001 0.0001 0.0005 0.0003 0.0001 0.0002 0.0002 0.0002 0.0017 0.0003 0.0007 0.0003 0.0009 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.8352 13.0129 15.4498 21.4479 19.5026 27.1576 19.2974 25.4568 26.5765 29.1507
Translational Efficiency 1.5988 1.4172 1.1234 0.9128 1.1855 1.3484 1.1537 0.7877 1.009 1.0482

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
41 765 2968 894 1530 1368 94 1098 1571 2133 3062 1992

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1040.04 710.01 752.11 771.75 624.16 672.50 883.13 847.05 635.01 685.95 756.13 813.26
Standard Deviation 318.43 105.92 93.07 132.92 76.87 84.47 163.69 137.98 113.13 94.46 98.64 140.80
Intensity Change Log 2 — -0.550728 -0.467623 -0.430434 — 0.107618 0.500710 0.440531 — -0.267539 -0.025326 -0.039904

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002203 0.000460 0.000086 0.000675 0.000100 0.000524 0.000284 0.006566 0.000155 0.000501 0.000092 0.003922
Bud Neck 0.021442 0.031580 0.011136 0.008771 0.013233 0.027949 0.001409 0.014987 0.013447 0.029252 0.010837 0.012197
Bud Site 0.052239 0.015592 0.013253 0.056546 0.004265 0.013959 0.001176 0.079041 0.005517 0.014544 0.012882 0.068945
Cell Periphery 0.001537 0.000194 0.000144 0.000308 0.000154 0.000143 0.000319 0.000249 0.000190 0.000162 0.000149 0.000275
Cytoplasm 0.348575* 0.372217* 0.388421* 0.403235* 0.486367* 0.469025* 0.176155* 0.403443* 0.482771* 0.434305* 0.381905* 0.403350*
Cytoplasmic Foci 0.082851 0.289712* 0.003818 0.031204 0.199932* 0.169558 0.046080 0.030036 0.196877* 0.212651* 0.005115 0.030560
Eisosomes 0.000147 0.000145 0.000016 0.000066 0.000131 0.000096 0.000168 0.000058 0.000131 0.000114 0.000020 0.000062
Endoplasmic Reticulum 0.023775 0.001071 0.001043 0.003715 0.000727 0.000841 0.003867 0.001942 0.001328 0.000924 0.001129 0.002737
Endosome 0.023713 0.022073 0.000695 0.007932 0.007277 0.009631 0.004577 0.017795 0.007706 0.014093 0.000815 0.013369
Golgi 0.007219 0.006612 0.000059 0.007633 0.001601 0.004027 0.000116 0.020493 0.001747 0.004954 0.000061 0.014722
Lipid Particles 0.015312 0.007708 0.000340 0.002393 0.009376 0.005621 0.005525 0.001908 0.009531 0.006370 0.000499 0.002126
Mitochondria 0.001511 0.012291 0.000832 0.001521 0.003017 0.011131 0.000351 0.008158 0.002978 0.011547 0.000817 0.005179
Mitotic Spindle 0.014733 0.004307 0.005749 0.040142 0.002027 0.006495 0.000584 0.094487 0.002359 0.005710 0.005590 0.070097
None 0.003751 0.004688 0.004584 0.008461 0.004233 0.003167 0.005857 0.004579 0.004220 0.003713 0.004623 0.006321
Nuclear Periphery 0.002852 0.000574 0.001633 0.001800 0.000688 0.000646 0.001679 0.001488 0.000744 0.000620 0.001634 0.001628
Nuclear Periphery Foci 0.059212 0.000831 0.000136 0.002619 0.000329 0.000443 0.001518 0.001566 0.001865 0.000582 0.000179 0.002039
Nucleolus 0.006942 0.002485 0.000539 0.000709 0.001127 0.001573 0.001958 0.000846 0.001279 0.001900 0.000583 0.000784
Nucleus 0.184992* 0.139498* 0.549534* 0.288482* 0.229806* 0.218398* 0.679513* 0.162771* 0.228637* 0.190100* 0.553524* 0.219190*
Peroxisomes 0.002122 0.025600 0.000102 0.001201 0.004269 0.009001 0.000714 0.002243 0.004213 0.014954 0.000120 0.001775
Vacuole 0.133611 0.060302 0.017307 0.128901 0.030682 0.046602 0.067936 0.138988 0.033368 0.051516 0.018861 0.134461
Vacuole Periphery 0.011260 0.002060 0.000573 0.003688 0.000660 0.001166 0.000213 0.008357 0.000936 0.001487 0.000562 0.006261

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.93 2.35 1.79 -1.53 -9.62 -2.11 -5.01 -2.61 -2.05 -1.86 -2.62 1.18 -2.96 -2.13 -3.04
Bud Neck -0.86 0.91 1.12 7.56 2.01 -6.40 8.44 2.58 8.12 -5.66 -7.85 2.21 3.66 11.21 1.25
Bud Site 1.76 1.96 0.91 -3.79 -4.84 -5.13 1.05 -8.82 -5.58 -9.13 -5.73 -3.87 -10.25 -6.67 -7.81
Cell Periphery 1.53 1.61 1.48 -1.68 -4.84 0.22 -4.15 0.05 -0.27 5.05 0.73 1.53 -0.04 -1.80 -3.36
Cytoplasm -0.56 -0.30 0.13 2.52 2.09 2.10 17.38 14.18 11.25 -9.17 5.66 15.97 16.36 10.78 1.82
Cytoplasmic Foci -11.19 4.81 3.65 28.97 -10.30 4.09 23.34 34.01 27.30 2.37 -2.06 37.61 33.99 38.39 -13.38
Eisosomes 0.13 4.13 2.29 8.15 -17.89 4.24 -2.17 10.06 5.16 6.99 2.26 18.37 9.83 8.82 -20.40
Endoplasmic Reticulum 3.47 3.41 2.93 -11.97 -11.39 -1.36 -6.65 -12.56 -11.18 4.61 1.66 -1.52 -9.21 -16.35 -11.89
Endosome 0.51 4.83 4.00 9.74 -6.98 -2.17 2.27 0.74 2.47 -1.17 -6.08 11.20 2.52 8.44 -8.56
Golgi 0.11 1.53 0.84 1.89 -2.20 -2.69 4.70 -2.59 -1.07 -3.57 -4.09 5.20 -2.51 0.21 -4.23
Lipid Particles 2.27 4.57 3.96 6.39 -9.54 3.56 4.21 9.02 5.81 5.01 3.30 10.89 9.05 8.28 -11.85
Mitochondria -5.20 1.07 0.39 5.53 -2.19 -4.83 7.24 -0.06 4.53 -3.77 -6.50 6.58 1.50 6.99 -3.74
Mitotic Spindle 1.13 0.91 -1.51 -6.11 -5.71 -2.90 -0.96 -9.53 -8.35 -8.22 -2.60 -3.12 -11.35 -10.24 -10.05
None -0.56 -0.58 -1.16 -0.76 -1.17 1.69 -0.75 -0.14 -2.41 0.72 0.81 -0.47 -1.00 -1.95 -0.80
Nuclear Periphery 3.42 1.22 -0.09 -13.90 -5.28 -0.01 -6.95 -12.93 -16.03 0.24 0.86 -11.73 -14.83 -21.09 -6.69
Nuclear Periphery Foci 2.62 2.65 2.53 -4.48 -6.76 -0.71 -5.37 -8.82 -5.38 2.07 2.01 2.61 -0.35 -6.74 -9.91
Nucleolus 1.63 2.49 2.44 3.29 -0.81 -1.72 -1.30 3.19 4.40 3.20 -2.24 3.53 3.91 5.14 -0.50
Nucleus 1.21 -10.82 -3.83 -16.14 23.69 0.99 -15.41 -0.54 -1.38 15.01 4.87 -45.94 -6.02 -11.02 38.57
Peroxisomes -9.81 5.02 3.50 10.58 -4.23 -4.41 7.92 7.23 8.69 -1.17 -9.29 9.21 7.65 13.51 -5.88
Vacuole 3.16 4.78 -2.75 -16.65 -23.00 -6.61 -5.37 -28.57 -25.87 -8.03 -8.00 2.31 -34.80 -30.76 -35.83
Vacuole Periphery 1.31 1.54 1.37 0.69 -1.86 -4.03 5.94 -2.49 -1.83 -3.08 -2.35 1.54 -2.27 -1.31 -3.07
ORF
Human Ortholog
Description Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YHR112C

YHR112C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhr112c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available