Standard name
Human Ortholog
Description Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.98 0.98 0.89 0.84 0.81 0.74 0.75 0.67 0.69 1.0 1.0 1.0 0.97 0.97 0.97 0.85 0.92 0.78 0.92 0.89 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0.05 0.08 0.28 0.37 0.29 0.42 0.47 0.53 0.5 0 0 0 0 0 0 0.05 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 5 0 1 2
Bud 1 0 1 1 0 12 14 9 15 21 25 25 0 0 0 0 0 0 1 1 6 0 6 10
Bud Neck 0 0 1 0 0 1 1 3 3 3 1 1 3 4 0 0 0 1 1 0 2 0 1 1
Bud Site 0 0 0 0 0 0 1 3 1 4 4 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 7 7 0 4 3 4 2 1 3 1 3 3 2 10 4 4 0 0 1 0 1 0
Cytoplasm 196 263 314 260 302 393 487 449 307 371 274 337 432 428 461 215 203 154 237 241 250 179 371 312
Endoplasmic Reticulum 0 0 1 0 0 1 0 0 0 0 1 2 0 1 1 13 17 11 3 0 4 1 2 0
Endosome 1 0 1 0 0 1 1 13 5 1 0 0 1 0 0 2 1 1 2 2 4 0 1 4
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3 0 0 1 0 0 0
Mitochondria 16 7 5 13 25 122 215 160 175 234 215 245 0 0 0 0 8 3 13 5 27 3 11 5
Nucleus 0 0 1 1 0 2 4 2 3 2 1 0 1 1 2 0 3 0 0 0 0 1 1 0
Nuclear Periphery 1 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 1 0 2 2 1 0 0 1 2 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 5 5 13 10 6 10 11 0 1 0 0 0 0 1 1 3 1 6 5
Vac/Vac Membrane 0 0 1 1 1 3 5 2 3 4 9 3 0 0 0 1 0 1 2 2 3 1 6 4
Unique Cell Count 207 268 323 265 309 441 578 552 414 494 409 491 433 429 462 221 210 159 278 263 322 194 417 354
Labelled Cell Count 217 271 335 285 328 546 738 660 525 648 545 634 440 438 466 242 238 178 278 263 322 194 417 354


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.3 4.3 4.3 4.2 3.6 3.5 3.5 3.4 3.1 2.9 3.1 5.8 5.6 5.3 6.1 5.5 5.7 5.7 5.8 6.1
Std Deviation (1e-4) 0.3 0.6 1.4 1.9 1.9 1.9 2.0 3.7 1.7 1.0 1.1 2.6 0.8 0.8 1.0 2.2 1.5 1.9 1.0 1.3 1.6
Intensity Change (Log2) – – – -0.0 -0.03 -0.25 -0.29 -0.29 -0.34 -0.48 -0.58 -0.46 0.42 0.38 0.31 0.5 0.35 0.4 0.41 0.44 0.51

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5557 -0.2265 -0.2723 -0.3237 -0.5608 -0.2656 0.8017 0.6155 0.6304 0.6267 1.2483 0.745 -0.2744 -0.1529 -0.4253 0.0348 0.0279 0.0227
Actin 0.0017 0.0006 0.0057 0.0002 0.0003 0.0003 0.0313 0.0006 0.0123 0.0031 0.0066 0.0046 0.0192 0.0007 0.0024 0.029 0.0297 0.0091
Bud 0.0001 0.0025 0.0011 0.0001 0.0001 0 0.0088 0.0053 0.002 0.001 0.0481 0.0036 0.0072 0.0155 0.0154 0.0018 0.0128 0.0021
Bud Neck 0.0079 0.0002 0.0009 0 0.0001 0.0003 0.0055 0.0003 0.0048 0.0011 0.0234 0.0067 0.0009 0.0003 0.0009 0.001 0.0008 0.0022
Bud Periphery 0.0001 0.0005 0.0008 0.0001 0.0002 0 0.0094 0.004 0.0021 0.0018 0.0138 0.0049 0.0025 0.0032 0.0071 0.0013 0.01 0.0015
Bud Site 0.0014 0.0045 0.0041 0 0 0 0.0082 0.0139 0.0142 0.0036 0.0288 0.0013 0.003 0.0278 0.0072 0.0014 0.0009 0.0013
Cell Periphery 0.0001 0.0002 0.0002 0 0 0 0.0021 0.0004 0.0003 0.0004 0.0005 0.0001 0.0004 0.0005 0.0005 0.0002 0.0001 0.0001
Cytoplasm 0.1124 0.127 0.1338 0.0858 0.1858 0.1673 0.1152 0.1969 0.1824 0.2667 0.1589 0.2612 0.1823 0.2867 0.28 0.2282 0.2484 0.3298
Cytoplasmic Foci 0.0162 0.0071 0.0071 0.0044 0.0073 0.0091 0.0094 0.0064 0.0103 0.0119 0.048 0.0124 0.0209 0.0065 0.0081 0.0159 0.0184 0.0035
Eisosomes 0.0002 0.0001 0.0002 0 0 0 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0003 0.0003 0.0001
Endoplasmic Reticulum 0.001 0.0009 0.0007 0.0005 0.0007 0.0007 0.0035 0.0008 0.0012 0.0014 0.001 0.001 0.003 0.0017 0.001 0.0042 0.0026 0.0013
Endosome 0.0031 0.0014 0.0013 0.0015 0.0037 0.0019 0.009 0.0017 0.0029 0.0124 0.0054 0.0032 0.0061 0.0025 0.0011 0.0208 0.0177 0.0019
Golgi 0.0006 0.0002 0.0008 0.0001 0.0001 0.0004 0.0029 0.0002 0.0012 0.0085 0.0045 0.0007 0.0024 0.0005 0.0002 0.003 0.0091 0.0014
Lipid Particles 0.0022 0.0009 0.0014 0.0001 0.0001 0.0023 0.0045 0.0002 0.005 0.0107 0.0079 0.0007 0.0062 0.0015 0.0002 0.0022 0.0231 0.0009
Mitochondria 0.0004 0.0005 0.004 0.0002 0.0003 0.0028 0.0021 0.0007 0.0009 0.0045 0.0041 0.0031 0.0013 0.0011 0.0015 0.0037 0.0029 0.0018
None 0.8421 0.8303 0.8267 0.9046 0.7989 0.8106 0.7637 0.7596 0.743 0.6626 0.6041 0.6853 0.7246 0.6444 0.6615 0.6593 0.5704 0.6376
Nuclear Periphery 0.0008 0.0004 0.0007 0.0003 0.0003 0.0003 0.0058 0.0004 0.0013 0.0015 0.0016 0.0015 0.0036 0.0008 0.001 0.0048 0.0084 0.0015
Nucleolus 0.0009 0.001 0.0003 0.0001 0.0001 0.0001 0.0021 0.0008 0.0007 0.0006 0.0037 0.0003 0.0008 0.0005 0.0008 0.0016 0.0014 0.0002
Nucleus 0.0016 0.0062 0.0017 0.0005 0.0007 0.0006 0.0039 0.0031 0.0049 0.0021 0.0177 0.0023 0.0038 0.003 0.0035 0.0092 0.0052 0.0016
Peroxisomes 0.004 0.0003 0.0067 0.0001 0.0001 0.0007 0.0051 0.0002 0.0045 0.0012 0.0095 0.0013 0.0031 0.0003 0.0002 0.0014 0.0136 0.0005
Punctate Nuclear 0.0024 0.0144 0.0014 0.0003 0.0005 0.0019 0.0034 0.0032 0.0044 0.0018 0.0107 0.0048 0.0068 0.0012 0.006 0.0079 0.0213 0.0011
Vacuole 0.0006 0.0007 0.0004 0.0009 0.0004 0.0004 0.0029 0.0013 0.001 0.0025 0.0013 0.0007 0.0012 0.001 0.0011 0.002 0.0018 0.0004
Vacuole Periphery 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.001 0.0002 0.0002 0.0009 0.0004 0.0003 0.0004 0.0002 0.0002 0.0008 0.001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.5476 24.4426 25.1584 37.0454 29.1612 17.6306 32.0882 38.7411 33.9851 35.6383
Translational Efficiency 0.9684 1.2584 1.2134 0.7187 0.9115 1.8656 0.9551 0.9273 1.0325 1.0048

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
747 260 1759 1278 567 1951 645 887 1314 2211 2404 2165

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 648.90 712.36 861.37 813.18 670.47 691.97 907.72 824.29 658.21 694.37 873.81 817.73
Standard Deviation 70.37 85.06 98.27 115.27 73.81 87.39 100.83 134.30 72.66 87.37 101.07 123.55
Intensity Change Log 2 — 0.134610 0.408637 0.325579 — 0.045537 0.437075 0.297979 — 0.090033 0.423158 0.311619

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000135 0.000769 0.000262 0.001520 0.000083 0.000460 0.000351 0.001822 0.000113 0.000496 0.000286 0.001644
Bud Neck 0.016838 0.030423 0.006471 0.013919 0.005041 0.040055 0.003678 0.015120 0.011747 0.038922 0.005722 0.014411
Bud Site 0.003996 0.021093 0.009516 0.049181 0.002083 0.020901 0.009475 0.038013 0.003171 0.020923 0.009505 0.044606
Cell Periphery 0.000134 0.000206 0.000191 0.000334 0.000157 0.000155 0.000221 0.000594 0.000144 0.000161 0.000199 0.000441
Cytoplasm 0.458742 0.363612 0.514040 0.490799 0.535736 0.437957 0.373503 0.466182 0.491965 0.429215 0.476334 0.480713
Cytoplasmic Foci 0.288711 0.306011 0.014043 0.028067 0.214999 0.256645 0.023139 0.036588 0.256904 0.262450 0.016483 0.031558
Eisosomes 0.000186 0.000194 0.000040 0.000068 0.000121 0.000163 0.000089 0.000185 0.000158 0.000166 0.000053 0.000116
Endoplasmic Reticulum 0.000413 0.000747 0.002390 0.002969 0.000712 0.001109 0.003893 0.001082 0.000542 0.001066 0.002793 0.002196
Endosome 0.015026 0.038844 0.002078 0.006753 0.009328 0.023511 0.004834 0.007667 0.012567 0.025314 0.002818 0.007127
Golgi 0.002485 0.003741 0.000065 0.004568 0.000798 0.007667 0.000220 0.003814 0.001757 0.007205 0.000106 0.004259
Lipid Particles 0.007508 0.006575 0.001298 0.001584 0.006380 0.006266 0.002706 0.003248 0.007021 0.006302 0.001676 0.002265
Mitochondria 0.005814 0.010406 0.000607 0.002867 0.001090 0.013585 0.000822 0.004261 0.003775 0.013211 0.000665 0.003438
Mitotic Spindle 0.000262 0.001409 0.005424 0.027563 0.001007 0.008070 0.006745 0.027024 0.000584 0.007287 0.005778 0.027342
None 0.001834 0.002646 0.004096 0.006319 0.003504 0.001652 0.006569 0.011490 0.002555 0.001769 0.004759 0.008437
Nuclear Periphery 0.000316 0.000281 0.001732 0.001036 0.000376 0.000289 0.001845 0.000834 0.000342 0.000288 0.001763 0.000953
Nuclear Periphery Foci 0.000252 0.000469 0.001020 0.001997 0.000505 0.000314 0.002439 0.001129 0.000361 0.000333 0.001401 0.001641
Nucleolus 0.000833 0.002201 0.000264 0.000284 0.000781 0.001202 0.000759 0.001541 0.000811 0.001319 0.000397 0.000799
Nucleus 0.130654 0.096481 0.371094 0.187878 0.171604 0.089146 0.433282 0.260347 0.148324 0.090009 0.387779 0.217568
Peroxisomes 0.006784 0.023654 0.000240 0.001396 0.002391 0.014113 0.000658 0.002738 0.004888 0.015235 0.000352 0.001946
Vacuole 0.058241 0.087528 0.064944 0.168934 0.042673 0.074168 0.123507 0.110519 0.051524 0.075739 0.080656 0.145001
Vacuole Periphery 0.000836 0.002711 0.000186 0.001967 0.000630 0.002572 0.001264 0.005803 0.000747 0.002589 0.000475 0.003538

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.01 -5.91 -4.75 -0.76 -3.66 -4.30 -8.08 -1.82 -1.14 -1.16 -4.81 -9.70 -3.57 -1.97 -2.60
Bud Neck -2.93 5.85 4.09 4.88 -1.35 -16.50 1.62 -3.68 12.09 -4.56 -12.93 5.41 1.82 14.21 -3.30
Bud Site -5.20 -3.42 -7.49 -0.95 -5.76 -11.19 -3.22 -7.32 -1.24 -5.24 -11.54 -4.91 -10.45 -1.61 -7.73
Cell Periphery -1.84 -2.40 -2.70 0.14 -0.75 0.35 -2.77 -3.46 -3.53 -2.72 -1.57 -2.84 -4.34 -3.66 -2.69
Cytoplasm 5.08 2.59 5.83 -1.38 4.05 8.30 17.81 9.71 3.09 -8.27 6.77 11.24 10.73 4.25 -0.27
Cytoplasmic Foci -1.06 34.05 33.22 20.31 -4.07 -4.70 26.53 24.98 43.32 -4.42 -0.74 42.64 40.95 49.85 -6.69
Eisosomes -0.18 11.37 9.86 3.72 -4.50 -4.06 2.44 -0.21 1.22 -0.92 -0.67 12.44 4.94 5.30 -2.36
Endoplasmic Reticulum -2.37 -17.10 -18.21 -11.80 1.03 -1.07 -15.11 -8.02 -2.60 11.12 -1.68 -21.74 -19.18 -5.55 7.28
Endosome -4.03 7.83 7.00 6.34 -2.39 -8.18 3.84 3.23 13.86 -0.41 -7.74 9.00 7.65 16.83 -2.56
Golgi -1.27 5.85 1.49 2.20 -2.55 -8.54 5.67 -1.56 5.56 -2.53 -7.27 6.93 0.26 6.74 -3.62
Lipid Particles 0.68 9.81 9.40 3.51 -2.11 0.23 6.40 5.61 5.76 0.64 1.25 12.28 10.90 8.75 -0.98
Mitochondria -1.30 3.17 2.68 2.98 -2.43 -10.19 1.88 -1.86 8.33 -2.69 -6.48 3.36 2.04 9.84 -3.59
Mitotic Spindle -3.19 -5.00 -6.87 -6.44 -4.52 -3.18 -1.01 -5.43 -3.71 -4.76 -4.63 -4.01 -8.69 -5.30 -6.40
None -0.53 -3.46 -3.05 -1.57 -1.32 1.85 -1.19 -2.02 -3.58 -1.40 1.63 -2.48 -3.35 -4.90 -2.16
Nuclear Periphery 0.76 -28.18 -22.14 -20.61 5.24 2.08 -21.18 -10.14 -10.71 5.95 1.60 -34.50 -22.16 -21.46 7.80
Nuclear Periphery Foci -2.86 -10.30 -12.01 -9.44 -4.10 2.19 -8.11 -5.83 -6.98 4.41 0.45 -12.31 -13.00 -12.08 -0.47
Nucleolus -1.37 3.88 3.54 1.91 -0.69 -3.27 0.63 -0.50 1.06 -0.78 -2.98 4.42 1.75 4.06 -1.43
Nucleus 3.09 -33.26 -12.31 -12.21 21.51 10.81 -22.52 -7.78 -19.54 15.42 11.21 -39.28 -14.12 -27.62 25.83
Peroxisomes -5.87 8.83 7.82 8.29 -2.16 -10.37 3.54 1.21 11.37 -2.14 -9.69 9.49 7.16 14.61 -3.16
Vacuole -4.04 -8.73 -27.48 -19.03 -21.71 -8.41 -16.25 -20.36 -17.10 -3.29 -7.67 -17.15 -34.09 -29.94 -20.24
Vacuole Periphery -2.71 7.05 0.67 2.82 -2.43 -5.95 0.12 -1.97 -0.20 -1.90 -6.25 3.79 -1.69 1.89 -2.66
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (33%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dma1

Dma1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dma1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available