Standard name
Human Ortholog
Description Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p; mutation in the human Orc6p is linked to Meier-Gorlin syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.08 0.08 0 0.05 0 0 0 0 0 0.12 0.15 0.2 0.15 0.09 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.05 0 0.07 0.13 0.39 0.36 0.31 0.32 0.4 0.51 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.9 0.87 0.76 0.6 0.6 0.61 0.55 0.61 0.55 0.53 0.55 0.85 0.8 0.76 0.81 0.86 0.83 0.81 0.8 0.66 0.64 0.59 0.47
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0.07 0.12 0.18 0.17 0.13 0.19 0.13 0.18 0.16 0.16 0 0 0 0 0 0 0.08 0.07 0.15 0.11 0.1 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0.06 0.12 0.16 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Bud 0 0 1 2 6 6 12 9 16 11 7 6 0 0 0 1 4 0 2 12 14 4 5 4
Bud Neck 0 0 0 1 3 0 2 2 4 1 0 1 0 1 0 0 0 0 0 2 1 0 0 3
Bud Site 3 0 0 1 6 13 23 15 11 8 16 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Cytoplasm 8 23 19 29 42 23 45 28 21 25 13 19 46 66 117 126 61 62 1 3 4 5 1 3
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 0 1 0 0 0 1 1 16 12 8 0 0 0 0 0 2
Endosome 2 1 28 6 35 6 22 34 30 27 13 16 0 5 6 3 4 0 3 3 15 11 19 33
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 3 1 1 0 2 7 1 3 9
Mitochondria 40 25 14 25 64 239 318 219 262 376 353 324 1 0 1 12 15 13 2 2 5 7 17 16
Nucleus 332 428 649 285 306 366 533 387 497 509 372 429 330 351 451 694 569 402 295 411 558 237 320 241
Nuclear Periphery 5 1 31 8 16 10 34 20 29 35 20 24 7 4 6 12 9 4 2 0 14 1 4 9
Nucleolus 23 13 51 45 93 106 117 133 103 169 112 123 4 8 13 6 7 5 28 37 130 39 53 65
Peroxisomes 0 1 4 1 5 0 0 1 1 1 0 3 0 1 1 0 2 0 0 0 0 0 0 0
SpindlePole 4 0 30 10 39 17 23 29 14 14 14 15 8 11 14 9 4 2 8 14 28 14 19 15
Vac/Vac Membrane 8 6 6 6 10 11 17 11 28 36 30 41 1 8 14 21 15 10 16 19 52 44 88 99
Unique Cell Count 365 476 745 374 511 612 873 698 817 932 698 784 386 438 597 856 658 486 364 516 843 373 541 517
Labelled Cell Count 425 498 834 421 625 797 1146 888 1016 1214 950 1011 397 456 625 903 705 507 364 516 843 373 541 517


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.7 6.3 5.7 5.2 4.5 4.4 4.4 4.4 4.5 4.1 4.3 7.5 7.4 7.1 9.0 7.9 8.3 7.6 7.5 8.1
Std Deviation (1e-4) 0.8 1.7 1.2 1.7 1.7 1.3 1.2 1.1 0.9 1.0 1.0 1.0 1.4 1.4 1.4 1.9 1.7 1.8 1.7 1.3 1.7
Intensity Change (Log2) – – – -0.14 -0.28 -0.49 -0.51 -0.53 -0.51 -0.49 -0.62 -0.57 0.24 0.22 0.16 0.52 0.33 0.39 0.27 0.25 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4.1 4.6 1.3 2.7 1.6 0 0.2 -0.9 -0.2 6.4 8.1 10.3 8.5 5.4 7.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.4 3.3 0
Endosome -2.0 2.5 0 0 0 0 0 0 0 -3.9 -2.6 -3.2 -4.9 -3.9 -4.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.1 7.7 0 0 0 0 0 0 0 -2.3 -2.9 -3.0 -0.8 0.5 0.9
Nucleus -4.6 -11.1 -11.5 -11.8 -13.4 -11.7 -14.3 -14.1 -13.9 -0.8 -3.2 -5.5 -3.3 -0.4 -2.1
Nuclear Periphery -1.7 -0.9 0 0 0 0 0 0 0 -2.1 -3.2 -3.5 -3.4 -3.1 -3.4
Nucleolus 2.9 6.2 0 0 0 0 0 0 0 -4.3 -3.8 -4.0 -6.6 -5.4 -4.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.2 2.8 0 0 0 0 0 0 0 -1.7 -1.4 -1.7 -3.9 -4.2 -3.9
Vacuole 0 1.8 1.6 1.9 1.4 3.5 4.0 4.3 5.0 0 1.6 2.3 2.6 2.3 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3064 2.6062 2.2723 2.1705 1.6075 2.1736 4.2949 2.3975 2.5776 2.4844 2.5714 2.0483 4.9141 4.4577 4.7397 4.6133 4.552 4.4818
Actin 0.0061 0.0001 0.0026 0.0003 0.0284 0.0007 0.0929 0 0.0141 0.037 0.0011 0.007 0.0149 0.0001 0.005 0.0015 0.0208 0.0002
Bud 0.0002 0.0001 0.0006 0 0.0004 0.0001 0.0012 0.0001 0.0012 0.0006 0 0.0002 0.001 0.002 0.0025 0.01 0.0008 0.0016
Bud Neck 0.0007 0.0003 0.0002 0.0004 0.0014 0.002 0.0067 0.0002 0.0035 0.0005 0.0044 0.0024 0.0007 0.0003 0.0003 0.0009 0.0015 0.0017
Bud Periphery 0.0003 0.0001 0.0001 0 0.0009 0.0003 0.0016 0.0001 0.0012 0.0012 0 0.0004 0.0008 0.0004 0.0004 0.002 0.0006 0.0006
Bud Site 0.0004 0.0008 0.0003 0.0001 0.0009 0.0002 0.0107 0.0002 0.0175 0.0017 0.0008 0.0004 0.0011 0.0012 0.0003 0.0015 0.0013 0.0002
Cell Periphery 0.0001 0.0001 0 0 0.0001 0.0001 0.0004 0 0.0007 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0001 0
Cytoplasm 0.0067 0.0089 0.0025 0.0012 0.0022 0.001 0.0265 0.0007 0.0074 0.0013 0.0114 0.0003 0.0023 0.0045 0.0027 0.0158 0.0272 0.0034
Cytoplasmic Foci 0.0035 0.0003 0.0018 0.0002 0.0004 0.0003 0.0136 0.0001 0.0081 0.0011 0.0014 0.0018 0.0055 0.0021 0.0002 0.0018 0.0037 0.0018
Eisosomes 0.0002 0 0 0.0001 0.0002 0.0004 0.0004 0 0.0002 0.0003 0.0001 0.0001 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.0016 0.0018 0.0004 0.0001 0.0005 0.0002 0.011 0.0001 0.0024 0.0003 0.0018 0.0003 0.0008 0.0001 0.0009 0.0005 0.0041 0.0001
Endosome 0.0057 0.0024 0.002 0.0003 0.0025 0.0025 0.0295 0.0001 0.0073 0.0013 0.0042 0.0045 0.0029 0.0001 0.0009 0.0053 0.0134 0.0002
Golgi 0.0006 0.0001 0.0005 0.0001 0.0036 0.0008 0.0082 0 0.0029 0.0016 0.0002 0.0024 0.0008 0 0.0002 0.0009 0.0016 0
Lipid Particles 0.0012 0.0004 0.0006 0.0003 0.0008 0.0004 0.0051 0 0.0081 0.0022 0.0004 0.0057 0.0016 0.0003 0.0001 0.0031 0.001 0.0001
Mitochondria 0.0018 0.0004 0.0005 0.0004 0.0047 0.0042 0.0051 0.0002 0.0049 0.0025 0.0003 0.0018 0.0052 0.0008 0.0008 0.0012 0.0009 0.0016
None 0.0223 0.0019 0.0129 0.051 0.0149 0.0121 0.0062 0.0005 0.0015 0.0011 0.0087 0.0004 0.0071 0.001 0.0006 0.004 0.0045 0.0013
Nuclear Periphery 0.0274 0.0096 0.0216 0.0298 0.0259 0.0043 0.0532 0.0034 0.0135 0.0099 0.017 0.0067 0.0091 0.0038 0.0119 0.0091 0.0279 0.0024
Nucleolus 0.0358 0.0284 0.021 0.0349 0.08 0.0891 0.0297 0.0372 0.0284 0.0287 0.0402 0.123 0.046 0.0438 0.0227 0.029 0.0498 0.0944
Nucleus 0.8498 0.913 0.8971 0.7914 0.8043 0.8402 0.6244 0.9215 0.8498 0.8402 0.7842 0.817 0.8217 0.8765 0.9333 0.8835 0.7562 0.8382
Peroxisomes 0.0007 0.0001 0.0003 0 0.0002 0.0001 0.0037 0 0.0082 0.0039 0.0001 0.0043 0.0019 0 0 0.0009 0.0003 0.0001
Punctate Nuclear 0.0321 0.0277 0.0342 0.0888 0.0265 0.0397 0.064 0.0352 0.0177 0.0635 0.1229 0.0202 0.0736 0.0622 0.0162 0.027 0.0822 0.0514
Vacuole 0.0015 0.0031 0.0003 0.0001 0.0006 0.0006 0.0041 0.0003 0.001 0.0006 0.0007 0.0007 0.0014 0.0004 0.0006 0.0011 0.001 0.0005
Vacuole Periphery 0.0014 0.0005 0.0004 0.0004 0.0006 0.0008 0.0019 0.0001 0.0004 0.0007 0.0002 0.0005 0.0009 0.0002 0.0005 0.0005 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.1704 12.0658 6.1554 9.9662 8.7499 8.4311 8.9591 12.8656 11.4167 11.4365
Translational Efficiency 1.7898 0.9776 1.7355 1.2545 1.254 1.2919 1.575 1.0024 1.401 1.2753

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p; mutation in the human Orc6p is linked to Meier-Gorlin syndrome
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Orc6

Orc6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Orc6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available