Standard name
Human Ortholog
Description sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0.06 0 0 0 0 – 0.05 0.06 0.08 0 0 0 0.1 0.07 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.61 0.45 0.68 0.69 0.67 0.61 0.55 – 0.64 0.74 0.56 0.62 0.52 0.51 0.72 0.62 0.7 0.14 0.14 0.05 0.07 0.09 0.06
Endoplasmic Reticulum 0.3 0.41 0.45 0.26 0.19 0.26 0.24 0.2 – 0.23 0.11 0.28 0.33 0.52 0.52 0 0 0 0.6 0.51 0.6 0.47 0.6 0.64
Endosome 0.09 0 0.11 0.06 0.07 0 0.07 0.08 – 0 0 0 0 0 0 0 0.14 0.11 0.05 0.05 0.12 0.12 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0
Mitochondria 0.06 0 0 0 0 0 0 0 – 0 0.07 0.05 0 0 0 0.1 0.08 0 0.05 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0.06 0 0.11 0.07 0.09 0.16 – 0.08 0.05 0.06 0.06 0 0 0 0.09 0.1 0.11 0.18 0.05 0.09 0.09 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 27 26 15 10 25 35 – 42 26 55 11 1 6 3 13 12 0 1 18 14 25 26
Bud 0 2 3 10 1 6 11 26 – 21 21 21 7 1 3 0 1 3 0 5 17 9 36 59
Bud Neck 0 0 0 1 0 0 0 1 – 0 0 0 0 0 3 0 0 0 0 1 2 3 3 4
Bud Site 0 0 0 0 0 0 0 0 – 2 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 6 7 6 6 – 18 11 23 0 3 14 0 2 2 0 0 0 0 0 2
Cytoplasm 28 67 194 298 393 354 462 428 – 504 319 388 266 281 421 21 112 118 14 18 26 29 71 66
Endoplasmic Reticulum 14 45 195 115 106 138 182 160 – 183 47 190 142 279 432 1 4 5 61 66 314 197 504 672
Endosome 4 0 48 26 40 17 52 62 – 25 8 18 7 0 2 1 26 19 4 6 60 49 43 48
Golgi 0 0 1 4 0 1 0 0 – 0 0 0 1 0 0 0 0 1 1 1 22 44 32 21
Mitochondria 3 3 3 3 7 11 20 15 – 29 31 34 0 0 0 3 14 2 4 4 17 22 27 30
Nucleus 0 0 0 0 1 0 0 3 – 2 1 0 1 2 0 1 2 1 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 17 – 1 0 7 0 1 1 0 2 0 0 0 4 4 8 9
Nucleolus 0 0 0 0 1 0 1 5 – 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 3 0 0 2 6 0 – 1 0 1 0 1 0 0 5 6 0 0 5 2 5 11
Vac/Vac Membrane 4 2 24 16 65 35 67 124 – 60 23 42 27 14 17 1 17 17 11 22 29 37 75 92
Unique Cell Count 47 109 430 440 567 529 756 782 787 431 688 432 541 830 29 181 168 102 130 528 421 843 1052
Labelled Cell Count 53 121 499 499 635 581 833 882 888 487 782 462 583 899 31 198 186 102 130 528 421 843 1052


Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 8.6 8.8 8.6 9.1 8.7 8.7 8.8 – 8.4 7.9 8.2 10.7 10.7 10.8 6.1 7.7 10.0 11.5 11.2 11.6
Std Deviation (1e-4) 2.8 2.5 2.4 2.7 2.6 2.4 2.3 2.0 – 2.0 1.6 1.7 2.3 2.5 2.3 1.6 1.9 2.2 3.0 2.8 3.1
Intensity Change (Log2) – – – -0.03 0.05 -0.01 -0.01 -0.0 – -0.06 -0.15 -0.1 0.29 0.29 0.29 -0.53 -0.18 0.19 0.39 0.35 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -2.8 -3.5 -2.4 -1.4 – -0.7 -0.2 1.1 -2.7 -5.6 -5.9 0 0.4 0.4
Bud 1.9 0 0 1.2 2.9 – 2.4 3.7 2.6 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 2.8 2.9 3.5 0 0 2.3 0 0 0
Cytoplasm 6.7 7.7 6.8 5.3 3.2 – 6.4 8.6 3.7 4.8 2.1 1.9 2.9 3.8 5.5
Endoplasmic Reticulum -5.9 -9.1 -6.2 -7.6 -9.1 – -8.0 -11.2 -6.1 -3.8 1.9 2.3 -4.4 -10.4 -9.9
Endosome -2.8 -2.3 -4.9 -2.6 -1.9 – -5.6 -5.5 -5.9 -5.7 -8.0 -9.4 0 1.1 0.1
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.8 2.3 1.7 – 3.1 4.9 3.9 0 0 0 0 4.8 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 3.1 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -1.4 3.2 0.7 2.0 5.2 – 1.3 -0.2 0.4 0.4 -2.4 -3.4 0 1.7 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2887 2.4505 2.2955 1.6568 1.7761 2.5781 0.3468 2.4837 1.4332 1.4237 0.3882 1.402 0.1038 0.878 0.2189 -0.1728 1.2928 0.3846
Actin 0.0079 0.0006 0.0005 0.0019 0.006 0.0085 0.0103 0.003 0.0218 0.0256 0.0027 0.0048 0.0399 0.0003 0.0057 0.0002 0.0105 0.0089
Bud 0.002 0.0013 0.0002 0.0031 0.0245 0.0003 0.0004 0.0014 0.0015 0.0026 0.0045 0.0002 0.001 0.0003 0.0002 0.0002 0.0024 0.0004
Bud Neck 0.0007 0.001 0.0003 0.0002 0.003 0.0082 0.0123 0.0004 0.0009 0.0004 0.0003 0.0003 0.0003 0.0005 0.0002 0.0002 0.0041 0.0005
Bud Periphery 0.0072 0.0011 0.0002 0.0385 0.0477 0.0012 0.0006 0.0028 0.0032 0.0064 0.0085 0.0003 0.0065 0.0002 0.0002 0.0002 0.0053 0.0011
Bud Site 0.0013 0.0116 0.0006 0.0003 0.0035 0.0002 0.0038 0.0052 0.002 0.0008 0.0021 0.0001 0.0011 0.0034 0.0005 0.0004 0.0093 0.0001
Cell Periphery 0.0011 0.0006 0.0002 0.0014 0.0005 0.0002 0.0006 0.0014 0.0005 0.0006 0.0005 0.0001 0.0005 0.0004 0.0002 0.0003 0.001 0.0002
Cytoplasm 0.1892 0.2612 0.2281 0.3383 0.0725 0.2362 0.2193 0.3198 0.2961 0.2281 0.1619 0.3044 0.0963 0.3309 0.2401 0.2068 0.2509 0.3055
Cytoplasmic Foci 0.0309 0.0319 0.0434 0.0513 0.0455 0.0237 0.0602 0.0732 0.0521 0.0343 0.0558 0.0398 0.0711 0.0726 0.0387 0.0481 0.0427 0.0397
Eisosomes 0.0003 0.0001 0.0001 0.0003 0.0002 0.0001 0.0003 0 0.0003 0.0002 0.0007 0.0001 0.0003 0 0.0001 0 0.0004 0.0001
Endoplasmic Reticulum 0.0804 0.0544 0.0412 0.0139 0.0331 0.0504 0.0521 0.0763 0.0204 0.0223 0.0125 0.0245 0.0748 0.0525 0.0538 0.0156 0.0154 0.0475
Endosome 0.0965 0.0774 0.1369 0.1262 0.1656 0.0942 0.1799 0.1488 0.1931 0.2866 0.2878 0.2296 0.2097 0.1987 0.2028 0.2224 0.229 0.1771
Golgi 0.0052 0.0036 0.011 0.017 0.0054 0.0045 0.0178 0.1073 0.0267 0.0112 0.0141 0.014 0.0248 0.0133 0.0249 0.0048 0.0103 0.0242
Lipid Particles 0.0213 0.0045 0.0035 0.0102 0.0054 0.0114 0.0225 0.0017 0.0056 0.002 0.0296 0.0039 0.0318 0.0139 0.0024 0.0027 0.0062 0.0174
Mitochondria 0.0053 0.0021 0.0053 0.0095 0.0025 0.0027 0.016 0.0066 0.0077 0.0054 0.007 0.0057 0.0046 0.0019 0.0054 0.0009 0.0085 0.0038
None 0.4821 0.5052 0.4813 0.3566 0.4021 0.4927 0.2971 0.2205 0.3154 0.3307 0.3534 0.3197 0.3452 0.2568 0.3779 0.4566 0.3287 0.308
Nuclear Periphery 0.0231 0.0087 0.0096 0.0026 0.0326 0.0204 0.0134 0.0048 0.0071 0.0034 0.0077 0.0085 0.0257 0.0098 0.0103 0.0038 0.0115 0.0125
Nucleolus 0.0007 0.0004 0.0003 0.0003 0.0046 0.0005 0.0013 0.0001 0.0003 0.0001 0.0013 0.0001 0.0004 0.0006 0.0001 0.0002 0.0046 0.0001
Nucleus 0.0072 0.0035 0.0023 0.0015 0.035 0.0094 0.0029 0.001 0.0019 0.0012 0.0021 0.001 0.0018 0.0022 0.0016 0.0012 0.0241 0.0014
Peroxisomes 0.0074 0.0007 0.0006 0.0013 0.003 0.0048 0.0057 0.0038 0.0018 0.0008 0.012 0.0023 0.0278 0.001 0.0005 0.0003 0.0007 0.0006
Punctate Nuclear 0.0022 0.0015 0.0013 0.0012 0.0367 0.0094 0.0137 0.0005 0.0012 0.0008 0.0019 0.0008 0.0061 0.0017 0.0006 0.0011 0.0108 0.0009
Vacuole 0.0241 0.0256 0.0287 0.021 0.0609 0.0175 0.0576 0.0168 0.0324 0.0267 0.0246 0.0293 0.0241 0.0344 0.0257 0.0295 0.0156 0.039
Vacuole Periphery 0.0036 0.003 0.0044 0.0034 0.0096 0.0036 0.0122 0.0046 0.008 0.0097 0.009 0.0104 0.006 0.0045 0.008 0.0044 0.008 0.011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.2098 16.7751 18.6718 27.3538 21.8971 14.0662 24.3819 20.8921 25.0976 40.6547
Translational Efficiency 1.2069 1.0933 0.8574 0.7212 1.0712 2.1472 0.9573 0.8161 0.8171 0.6767

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication
Localization
Cell Percentages ER (39%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Ept1

Ept1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ept1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available