ORF
Human Ortholog
Description Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0.05 0 0 0.07 0 0 0 0 0.05 0.1 0.14 0 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.3 0 0 0 0.05 0.06 0 0.14 0.08 0.11 0.16 0 0 0 0.09 0 0 0 0 0 0 0 0
Nucleus 0.79 0.64 0.75 0.69 0.76 0.74 0.67 0.64 0.59 0.59 0.62 0.52 0.65 0.65 0.67 0.63 0.52 0.57 0.49 0.52 0.55 0.57 0.58 0.45
Nuclear Periphery 0 0.07 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.37 0.41 0.24 0.26 0.22 0.26 0.28 0.31 0.41 0.45 0.43 0.52 0.38 0.28 0.21 0.27 0.39 0.35 0.3 0.28 0.3 0.3 0.24 0.32
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0.05 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0.06 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 14 0 0 1 2 2 0 0 0 0 0 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 2 3 0 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3 5 1 3 2 4 5 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 6 15 8 9 8 23 9 6 13 6 5 24 44 2 9 7 0 0 0 0 2 0
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 2 2 0 0 0 0 1 0
Endosome 0 0 2 0 2 6 3 0 0 2 0 0 0 1 3 1 1 5 4 2 3 6 5 14
Golgi 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 0 2
Mitochondria 0 13 2 8 3 12 17 8 43 32 41 51 4 2 1 8 2 4 0 2 2 0 2 6
Nucleus 15 28 135 127 133 196 205 218 180 226 231 165 64 154 207 59 68 85 32 43 120 105 153 110
Nuclear Periphery 0 3 5 4 8 5 7 12 8 12 10 6 3 7 7 4 3 5 1 3 5 1 2 5
Nucleolus 7 18 43 47 39 69 85 105 126 173 161 167 38 67 64 25 51 52 19 23 67 55 62 78
Peroxisomes 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 9 4 1 6 11 4 2 4 4 5 1 4 12 0 2 1 3 2 11 4 12 14
Vac/Vac Membrane 0 0 2 0 0 5 3 12 2 4 3 3 0 2 3 6 8 8 3 2 6 4 13 10
Unique Cell Count 19 44 180 183 175 265 306 342 305 384 371 320 99 237 308 94 130 148 66 83 221 183 262 247
Labelled Cell Count 22 62 204 207 194 312 367 387 373 462 471 413 115 262 343 106 146 170 66 83 221 183 262 247


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 5.6 7.6 7.6 8.4 7.5 7.3 7.8 7.0 7.0 6.9 6.9 7.1 7.8 7.7 7.9 10.0 10.4 8.4 8.4 8.3
Std Deviation (1e-4) 1.3 1.4 1.1 1.6 1.5 1.5 1.8 1.6 1.4 1.5 1.6 1.6 1.7 1.9 2.4 3.3 2.7 3.2 1.4 1.6 1.7
Intensity Change (Log2) – – – 0.01 0.15 -0.0 -0.05 0.04 -0.12 -0.11 -0.13 -0.14 -0.09 0.04 0.02 0.06 0.4 0.45 0.15 0.16 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2.9 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 0.6 0 -0.5 1.6 -0.2 0 0.1 0 0 2.7 3.8 0 1.5 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.0 2.4 0 4.8 3.4 4.1 5.2 0 0 0 0 0 0
Nucleus -1.2 0.2 -0.2 -1.9 -2.6 -3.6 -3.7 -3.0 -5.1 -1.8 -2.2 -1.8 -2.1 -4.1 -3.4
Nuclear Periphery 0 0.9 0 0 0.4 0 0.2 0 0 0 0.1 0 0 0 0
Nucleolus 0.4 -0.4 0.5 0.9 1.6 3.9 4.8 4.4 6.2 2.6 1.0 -0.8 0.5 2.9 2.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.4 0 -1.6 -0.8 0 0 0 0 -2.2 0 -1.9 -0.6 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.176 4.8067 4.7558 3.9621 4.3987 4.7528 5.6818 6.0682 6.3258 5.4942 4.8465 5.8125 3.958 4.4613 4.163 4.0904 3.7548 3.9232
Actin 0.0001 0.0002 0.0003 0.0001 0.0005 0.0001 0.0437 0.0001 0.0336 0.005 0.0252 0.0075 0.0264 0.0001 0.0171 0.063 0.001 0.0001
Bud 0.0001 0.0002 0 0 0 0 0.0013 0 0.0003 0.0002 0.0002 0.0001 0.0004 0 0.0004 0.0002 0.0002 0.0001
Bud Neck 0.0002 0.0002 0.0002 0.0002 0.0003 0.0009 0.0005 0.0001 0.0004 0.0002 0.0011 0.0008 0.003 0.0003 0.0004 0.0007 0.0007 0.0013
Bud Periphery 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0012 0 0.0003 0.0002 0.0011 0.0001 0.0006 0.0001 0.0009 0.0003 0.0003 0.0003
Bud Site 0.0001 0.0019 0.0001 0 0.0001 0.0001 0.0031 0 0.0013 0.0007 0.0004 0.0001 0.0037 0.0001 0.001 0.0004 0.0009 0.0001
Cell Periphery 0 0 0 0 0 0 0.0001 0 0.0001 0 0.0005 0 0.0003 0 0.0003 0.0001 0.0001 0
Cytoplasm 0 0.0003 0.0001 0 0.0001 0 0.0019 0.0001 0.0014 0.0011 0.0013 0.0004 0.0043 0.0001 0.0003 0.0002 0.0003 0
Cytoplasmic Foci 0 0.0001 0 0.0001 0.0003 0 0.0082 0 0.0065 0.0213 0.0278 0.002 0.0159 0 0.0009 0.0006 0.0045 0
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0.0003 0.0006 0.0001 0
Endoplasmic Reticulum 0 0 0 0 0.0001 0 0.0003 0.0001 0.002 0.0006 0.0005 0.0003 0.0015 0 0.0004 0.001 0.0003 0
Endosome 0 0 0.0001 0.0001 0.0004 0 0.0055 0.0001 0.0175 0.019 0.0101 0.0029 0.0091 0.0001 0.0014 0.0019 0.0098 0
Golgi 0 0 0 0 0.0002 0 0.0103 0 0.0127 0.0102 0.0064 0.0012 0.0051 0 0.0014 0.0033 0.0079 0
Lipid Particles 0 0.0001 0.0001 0.0004 0.0023 0 0.0007 0 0.0325 0.0051 0.0238 0.0015 0.0218 0 0.0022 0.0015 0.0191 0
Mitochondria 0.0007 0.0003 0.0003 0.0001 0.0005 0.0001 0.0037 0.0007 0.0059 0.0018 0.005 0.0006 0.0068 0.0005 0.0055 0.0011 0.0104 0.0003
None 0 0.0001 0.0001 0 0.0002 0 0.0001 0 0.0006 0.0006 0.0029 0.0002 0.0052 0 0.0003 0.0007 0.001 0
Nuclear Periphery 0.0091 0.049 0.0553 0.026 0.0662 0.0049 0.0017 0.0414 0.0496 0.0915 0.05 0.004 0.0212 0.0272 0.019 0.0185 0.0832 0.0067
Nucleolus 0.2163 0.1024 0.1365 0.1897 0.3608 0.3554 0.2217 0.0772 0.1219 0.0818 0.3341 0.2441 0.1686 0.0944 0.14 0.1332 0.2407 0.294
Nucleus 0.7534 0.8265 0.7769 0.7532 0.5156 0.6324 0.6918 0.8724 0.6602 0.6282 0.4124 0.7251 0.662 0.8694 0.7949 0.7501 0.5182 0.6926
Peroxisomes 0 0 0 0 0.0001 0 0.0027 0 0.0299 0.0111 0.0404 0.0018 0.0082 0 0.0004 0.0005 0.0025 0
Punctate Nuclear 0.0189 0.0179 0.0295 0.0294 0.0507 0.0056 0.0009 0.0065 0.0213 0.1201 0.0466 0.0069 0.0322 0.0064 0.0103 0.0217 0.0959 0.0039
Vacuole 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0006 0.0002 0.0037 0.0001 0.002 0.0003 0.0016 0.0001 0.0009 0.0002
Vacuole Periphery 0.0004 0.0005 0.0004 0.0003 0.0011 0.0001 0.0002 0.001 0.0014 0.0009 0.0062 0.0001 0.0013 0.0008 0.0011 0.0003 0.0021 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.453 6.7375 34.9968 28.7778 47.8825 57.2488 22.14 19.7708 32.8806 35.1013
Translational Efficiency 2.1696 6.101 1.1173 1.5609 1.4573 1.6312 1.7018 1.8483 1.2568 1.8257

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1494 1318 2345 1818 1311 2361 2009 1287 2805 3679 4354 3105

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 671.18 919.82 1167.86 1195.66 768.26 972.74 1104.66 1172.44 716.55 953.78 1138.70 1186.04
Standard Deviation 99.72 142.48 180.04 215.05 142.02 129.72 176.28 202.72 130.65 136.81 181.08 210.34
Intensity Change Log 2 — 0.454652 0.799096 0.833036 — 0.340460 0.523936 0.609848 — 0.394831 0.658785 0.718222

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000758 0.001230 0.000744 0.001032 0.000463 0.000392 0.000878 0.000954 0.000620 0.000692 0.000805 0.001000
Bud Neck 0.001071 0.004320 0.006838 0.014490 0.002243 0.003188 0.008064 0.008770 0.001619 0.003594 0.007404 0.012119
Bud Site 0.000776 0.001266 0.000463 0.002192 0.000362 0.000478 0.000786 0.000911 0.000583 0.000760 0.000612 0.001661
Cell Periphery 0.000129 0.000080 0.000020 0.000067 0.001011 0.000029 0.000038 0.000036 0.000541 0.000047 0.000028 0.000054
Cytoplasm 0.005049 0.002574 0.000121 0.000652 0.006388 0.000335 0.000309 0.000698 0.005674 0.001137 0.000207 0.000671
Cytoplasmic Foci 0.002314 0.001010 0.000102 0.002462 0.002376 0.000671 0.000129 0.000112 0.002343 0.000792 0.000114 0.001488
Eisosomes 0.000044 0.000042 0.000039 0.000034 0.002869 0.000028 0.000043 0.000036 0.001364 0.000033 0.000041 0.000035
Endoplasmic Reticulum 0.003045 0.003675 0.000583 0.001290 0.001913 0.001510 0.001397 0.000862 0.002516 0.002286 0.000958 0.001112
Endosome 0.000382 0.000668 0.000058 0.003378 0.000253 0.001185 0.000194 0.000243 0.000322 0.001000 0.000121 0.002079
Golgi 0.001164 0.001046 0.000220 0.002391 0.000741 0.001214 0.000252 0.000783 0.000966 0.001154 0.000235 0.001725
Lipid Particles 0.001442 0.000886 0.000038 0.000127 0.002112 0.000328 0.000048 0.000042 0.001755 0.000528 0.000043 0.000092
Mitochondria 0.003303 0.002395 0.001677 0.005103 0.001024 0.002968 0.001409 0.001943 0.002238 0.002763 0.001554 0.003793
Mitotic Spindle 0.006263 0.045681 0.006390 0.036904 0.013628 0.011693 0.013918 0.018649 0.009705 0.023869 0.009863 0.029338
None 0.025111 0.002596 0.000608 0.001613 0.029758 0.001354 0.003041 0.001807 0.027282 0.001799 0.001730 0.001693
Nuclear Periphery 0.000820 0.001451 0.000436 0.002635 0.000467 0.000909 0.000489 0.000977 0.000655 0.001104 0.000461 0.001948
Nuclear Periphery Foci 0.029402 0.023709 0.000459 0.001696 0.013401 0.007029 0.001076 0.001167 0.021924 0.013004 0.000744 0.001477
Nucleolus 0.384868* 0.363072* 0.358290* 0.308566* 0.390629* 0.381114* 0.321123* 0.377372* 0.387561* 0.374651* 0.341141* 0.337086*
Nucleus 0.531510* 0.540009* 0.619372* 0.607577* 0.526979* 0.583543* 0.643183* 0.577854* 0.529392* 0.567947* 0.630358* 0.595257*
Peroxisomes 0.001913 0.002926 0.002663 0.002440 0.002898 0.000835 0.002814 0.003226 0.002373 0.001584 0.002733 0.002766
Vacuole 0.000346 0.000891 0.000247 0.002992 0.000330 0.000648 0.000501 0.002499 0.000339 0.000735 0.000364 0.002788
Vacuole Periphery 0.000291 0.000472 0.000634 0.002358 0.000153 0.000550 0.000309 0.001061 0.000227 0.000522 0.000484 0.001821

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.96 -0.37 -6.35 0.85 -7.03 2.31 -10.25 -9.83 -14.53 -1.94 -1.37 -5.42 -11.12 -9.83 -6.99
Bud Neck -8.53 -20.70 -18.67 -13.53 -10.88 -2.92 -16.17 -11.10 -9.84 -1.79 -8.74 -25.59 -21.45 -17.44 -10.04
Bud Site -1.68 1.53 -4.50 -2.62 -5.90 -0.63 -6.27 -5.60 -4.49 -1.48 -1.12 -0.94 -5.89 -4.95 -5.97
Cell Periphery 2.20 8.81 4.66 0.81 -7.00 4.50 4.45 4.45 -1.45 -0.03 4.83 5.03 4.76 -0.93 -5.94
Cytoplasm 1.77 5.80 5.00 2.18 -4.59 5.01 5.07 4.72 -1.30 -1.75 5.53 7.62 6.83 1.41 -4.50
Cytoplasmic Foci 2.07 4.22 0.58 -1.35 -3.25 2.30 2.94 2.95 2.77 0.11 3.33 4.89 2.20 -1.13 -3.23
Eisosomes 0.88 4.04 5.75 4.05 2.76 3.16 3.14 3.15 -5.97 6.13 3.16 3.14 3.16 -1.38 5.87
Endoplasmic Reticulum -2.48 13.49 7.72 8.38 -6.71 1.73 2.18 4.34 3.80 5.76 1.01 10.80 8.32 7.59 -3.06
Endosome -1.34 5.47 -3.40 -2.77 -4.01 -2.29 1.38 0.48 2.38 -0.68 -2.50 4.86 -3.35 -1.21 -4.04
Golgi 0.36 4.11 -0.90 -1.17 -3.23 -1.10 5.53 0.28 1.00 -1.17 -0.47 5.63 -0.76 -0.35 -3.39
Lipid Particles 1.40 5.29 4.99 2.95 -2.07 2.86 3.41 3.41 2.59 0.10 3.61 5.62 5.46 3.73 -2.01
Mitochondria 1.54 2.56 -1.77 -4.10 -5.65 -3.20 -6.47 -11.10 1.24 -6.55 -0.80 1.87 -3.20 -2.06 -6.53
Mitotic Spindle -9.55 -0.64 -10.13 0.65 -9.90 1.66 0.17 -1.98 -3.58 -2.26 -6.41 -0.20 -9.14 -3.70 -9.30
None 12.21 13.31 12.66 3.21 -11.71 7.44 6.98 7.32 -1.31 1.54 12.74 12.74 12.74 -0.08 -0.64
Nuclear Periphery -2.99 1.99 -6.39 -4.31 -7.81 -3.06 -0.46 -2.45 -0.46 -2.23 -3.50 1.63 -6.95 -4.57 -7.94
Nuclear Periphery Foci 1.57 11.15 10.67 9.42 -3.73 3.66 7.24 7.17 6.19 0.07 4.85 13.25 12.80 11.14 -2.60
Nucleolus 2.91 2.66 9.18 6.38 7.14 1.19 7.33 1.25 0.35 -5.25 2.40 6.96 7.98 6.31 1.85
Nucleus -0.90 -10.39 -7.66 -6.73 1.48 -7.43 -12.17 -4.62 1.51 6.40 -6.87 -15.92 -8.94 -3.24 5.30
Peroxisomes -3.13 -3.01 -1.67 1.84 1.23 3.09 -0.02 -0.61 -16.56 -1.27 2.26 -1.15 -1.07 -6.44 0.22
Vacuole -2.10 0.63 -4.53 -2.90 -4.81 -1.17 -0.81 -2.31 -2.03 -2.11 -2.35 -0.21 -4.67 -3.74 -4.65
Vacuole Periphery -1.47 -1.24 -3.85 -3.25 -2.71 -2.39 -2.19 -2.81 -1.29 -2.22 -2.48 -1.73 -4.75 -3.36 -3.49
ORF
Human Ortholog
Description Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis
Localization
Cell Percentages nucleus (65%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

YHR127W

YHR127W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhr127w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available