ORF | |
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Human Ortholog | |
Description | Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 1 | 3 | 2 | 4 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 6 | 15 | 8 | 9 | 8 | 23 | 9 | 6 | 13 | 6 | 5 | 24 | 44 | 2 | 9 | 7 | 0 | 0 | 0 | 0 | 2 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 0 | 0 | 2 | 0 | 2 | 6 | 3 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 5 | 4 | 2 | 3 | 6 | 5 | 14 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 |
Mitochondria | 0 | 13 | 2 | 8 | 3 | 12 | 17 | 8 | 43 | 32 | 41 | 51 | 4 | 2 | 1 | 8 | 2 | 4 | 0 | 2 | 2 | 0 | 2 | 6 |
Nucleus | 15 | 28 | 135 | 127 | 133 | 196 | 205 | 218 | 180 | 226 | 231 | 165 | 64 | 154 | 207 | 59 | 68 | 85 | 32 | 43 | 120 | 105 | 153 | 110 |
Nuclear Periphery | 0 | 3 | 5 | 4 | 8 | 5 | 7 | 12 | 8 | 12 | 10 | 6 | 3 | 7 | 7 | 4 | 3 | 5 | 1 | 3 | 5 | 1 | 2 | 5 |
Nucleolus | 7 | 18 | 43 | 47 | 39 | 69 | 85 | 105 | 126 | 173 | 161 | 167 | 38 | 67 | 64 | 25 | 51 | 52 | 19 | 23 | 67 | 55 | 62 | 78 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 9 | 4 | 1 | 6 | 11 | 4 | 2 | 4 | 4 | 5 | 1 | 4 | 12 | 0 | 2 | 1 | 3 | 2 | 11 | 4 | 12 | 14 |
Vac/Vac Membrane | 0 | 0 | 2 | 0 | 0 | 5 | 3 | 12 | 2 | 4 | 3 | 3 | 0 | 2 | 3 | 6 | 8 | 8 | 3 | 2 | 6 | 4 | 13 | 10 |
Unique Cell Count | 19 | 44 | 180 | 183 | 175 | 265 | 306 | 342 | 305 | 384 | 371 | 320 | 99 | 237 | 308 | 94 | 130 | 148 | 66 | 83 | 221 | 183 | 262 | 247 |
Labelled Cell Count | 22 | 62 | 204 | 207 | 194 | 312 | 367 | 387 | 373 | 462 | 471 | 413 | 115 | 262 | 343 | 106 | 146 | 170 | 66 | 83 | 221 | 183 | 262 | 247 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 5.6 | 7.6 | 7.6 | 8.4 | 7.5 | 7.3 | 7.8 | 7.0 | 7.0 | 6.9 | 6.9 | 7.1 | 7.8 | 7.7 | 7.9 | 10.0 | 10.4 | 8.4 | 8.4 | 8.3 |
Std Deviation (1e-4) | 1.3 | 1.4 | 1.1 | 1.6 | 1.5 | 1.5 | 1.8 | 1.6 | 1.4 | 1.5 | 1.6 | 1.6 | 1.7 | 1.9 | 2.4 | 3.3 | 2.7 | 3.2 | 1.4 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.15 | -0.0 | -0.05 | 0.04 | -0.12 | -0.11 | -0.13 | -0.14 | -0.09 | 0.04 | 0.02 | 0.06 | 0.4 | 0.45 | 0.15 | 0.16 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.0 | 0.6 | 0 | -0.5 | 1.6 | -0.2 | 0 | 0.1 | 0 | 0 | 2.7 | 3.8 | 0 | 1.5 | 0.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.0 | 2.4 | 0 | 4.8 | 3.4 | 4.1 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.2 | 0.2 | -0.2 | -1.9 | -2.6 | -3.6 | -3.7 | -3.0 | -5.1 | -1.8 | -2.2 | -1.8 | -2.1 | -4.1 | -3.4 |
Nuclear Periphery | 0 | 0.9 | 0 | 0 | 0.4 | 0 | 0.2 | 0 | 0 | 0 | 0.1 | 0 | 0 | 0 | 0 |
Nucleolus | 0.4 | -0.4 | 0.5 | 0.9 | 1.6 | 3.9 | 4.8 | 4.4 | 6.2 | 2.6 | 1.0 | -0.8 | 0.5 | 2.9 | 2.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -1.4 | 0 | -1.6 | -0.8 | 0 | 0 | 0 | 0 | -2.2 | 0 | -1.9 | -0.6 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.176 | 4.8067 | 4.7558 | 3.9621 | 4.3987 | 4.7528 | 5.6818 | 6.0682 | 6.3258 | 5.4942 | 4.8465 | 5.8125 | 3.958 | 4.4613 | 4.163 | 4.0904 | 3.7548 | 3.9232 |
Actin | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0437 | 0.0001 | 0.0336 | 0.005 | 0.0252 | 0.0075 | 0.0264 | 0.0001 | 0.0171 | 0.063 | 0.001 | 0.0001 |
Bud | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0.0013 | 0 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0004 | 0.0002 | 0.0002 | 0.0001 |
Bud Neck | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0009 | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0011 | 0.0008 | 0.003 | 0.0003 | 0.0004 | 0.0007 | 0.0007 | 0.0013 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0012 | 0 | 0.0003 | 0.0002 | 0.0011 | 0.0001 | 0.0006 | 0.0001 | 0.0009 | 0.0003 | 0.0003 | 0.0003 |
Bud Site | 0.0001 | 0.0019 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0031 | 0 | 0.0013 | 0.0007 | 0.0004 | 0.0001 | 0.0037 | 0.0001 | 0.001 | 0.0004 | 0.0009 | 0.0001 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.0019 | 0.0001 | 0.0014 | 0.0011 | 0.0013 | 0.0004 | 0.0043 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0 |
Cytoplasmic Foci | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0082 | 0 | 0.0065 | 0.0213 | 0.0278 | 0.002 | 0.0159 | 0 | 0.0009 | 0.0006 | 0.0045 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0003 | 0.0006 | 0.0001 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.002 | 0.0006 | 0.0005 | 0.0003 | 0.0015 | 0 | 0.0004 | 0.001 | 0.0003 | 0 |
Endosome | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0055 | 0.0001 | 0.0175 | 0.019 | 0.0101 | 0.0029 | 0.0091 | 0.0001 | 0.0014 | 0.0019 | 0.0098 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0103 | 0 | 0.0127 | 0.0102 | 0.0064 | 0.0012 | 0.0051 | 0 | 0.0014 | 0.0033 | 0.0079 | 0 |
Lipid Particles | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0023 | 0 | 0.0007 | 0 | 0.0325 | 0.0051 | 0.0238 | 0.0015 | 0.0218 | 0 | 0.0022 | 0.0015 | 0.0191 | 0 |
Mitochondria | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0037 | 0.0007 | 0.0059 | 0.0018 | 0.005 | 0.0006 | 0.0068 | 0.0005 | 0.0055 | 0.0011 | 0.0104 | 0.0003 |
None | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0006 | 0.0006 | 0.0029 | 0.0002 | 0.0052 | 0 | 0.0003 | 0.0007 | 0.001 | 0 |
Nuclear Periphery | 0.0091 | 0.049 | 0.0553 | 0.026 | 0.0662 | 0.0049 | 0.0017 | 0.0414 | 0.0496 | 0.0915 | 0.05 | 0.004 | 0.0212 | 0.0272 | 0.019 | 0.0185 | 0.0832 | 0.0067 |
Nucleolus | 0.2163 | 0.1024 | 0.1365 | 0.1897 | 0.3608 | 0.3554 | 0.2217 | 0.0772 | 0.1219 | 0.0818 | 0.3341 | 0.2441 | 0.1686 | 0.0944 | 0.14 | 0.1332 | 0.2407 | 0.294 |
Nucleus | 0.7534 | 0.8265 | 0.7769 | 0.7532 | 0.5156 | 0.6324 | 0.6918 | 0.8724 | 0.6602 | 0.6282 | 0.4124 | 0.7251 | 0.662 | 0.8694 | 0.7949 | 0.7501 | 0.5182 | 0.6926 |
Peroxisomes | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0027 | 0 | 0.0299 | 0.0111 | 0.0404 | 0.0018 | 0.0082 | 0 | 0.0004 | 0.0005 | 0.0025 | 0 |
Punctate Nuclear | 0.0189 | 0.0179 | 0.0295 | 0.0294 | 0.0507 | 0.0056 | 0.0009 | 0.0065 | 0.0213 | 0.1201 | 0.0466 | 0.0069 | 0.0322 | 0.0064 | 0.0103 | 0.0217 | 0.0959 | 0.0039 |
Vacuole | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0037 | 0.0001 | 0.002 | 0.0003 | 0.0016 | 0.0001 | 0.0009 | 0.0002 |
Vacuole Periphery | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.0011 | 0.0001 | 0.0002 | 0.001 | 0.0014 | 0.0009 | 0.0062 | 0.0001 | 0.0013 | 0.0008 | 0.0011 | 0.0003 | 0.0021 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.453 | 6.7375 | 34.9968 | 28.7778 | 47.8825 | 57.2488 | 22.14 | 19.7708 | 32.8806 | 35.1013 |
Translational Efficiency | 2.1696 | 6.101 | 1.1173 | 1.5609 | 1.4573 | 1.6312 | 1.7018 | 1.8483 | 1.2568 | 1.8257 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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