Standard name
Human Ortholog
Description Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; forms actin-like short filament composed of 9 or 10 Arp1p monomers; putative ortholog of mammalian centractin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.95 0.97 0.94 0.92 0.86 0.74 0.74 0.62 0.68 0.52 0.51 0.98 0.98 0.99 0.9 0.94 0.91 0.87 0.92 0.83 0.88 0.85 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.06 0 0.17 0.13 0.31 0.49 0.39 0.52 0.61 0.61 0.68 0 0 0 0 0 0 0 0 0.07 0 0 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 2 0 1 1 1 0 2 1
Bud 1 0 1 0 3 4 4 7 9 7 7 8 0 0 0 0 1 0 0 0 1 3 3 4
Bud Neck 0 0 0 0 1 1 0 1 0 1 0 0 0 0 0 1 1 1 1 0 1 1 2 0
Bud Site 0 0 0 0 1 0 3 1 1 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 0 3 2 1 0 2 7 1 3 3 2 2 1 2 14 11 9 0 0 0 0 0 0
Cytoplasm 164 60 182 190 283 198 249 309 157 181 114 139 286 268 383 261 250 278 162 74 166 185 215 186
Endoplasmic Reticulum 0 0 0 0 1 1 3 1 1 1 1 1 0 0 1 13 15 26 0 0 0 3 0 0
Endosome 0 0 3 2 3 5 2 11 5 2 0 2 1 2 1 8 8 11 3 0 4 1 1 11
Golgi 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 2 2 3 2 0 1 2 1 2
Mitochondria 15 4 3 35 40 72 167 163 132 163 134 186 0 1 0 4 11 9 4 1 14 1 10 17
Nucleus 0 1 0 0 3 1 0 0 1 0 0 1 1 1 1 7 1 5 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 1 0 0 1 0 2 1 0 0 0 1 2 0 0 0 0 0 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 2
SpindlePole 0 0 1 0 3 1 3 1 4 2 1 4 0 2 1 7 4 3 1 0 2 2 4 3
Vac/Vac Membrane 2 0 1 2 1 0 7 8 8 5 9 8 2 4 0 8 0 12 6 0 4 3 4 8
Unique Cell Count 175 63 188 203 306 231 338 415 252 266 218 273 292 274 387 291 266 305 188 81 199 211 253 246
Labelled Cell Count 186 65 194 232 341 287 443 509 319 367 272 355 294 279 390 325 306 360 188 81 199 211 253 246


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.1 4.1 3.9 4.4 3.9 3.8 3.9 3.6 3.7 3.8 3.4 5.9 5.8 5.6 6.0 5.7 5.8 6.6 6.1 6.3
Std Deviation (1e-4) 0.5 0.5 0.6 0.8 1.9 1.4 1.9 1.7 1.6 2.0 2.4 1.7 1.2 1.3 1.1 1.5 1.3 1.5 9.7 1.4 1.9
Intensity Change (Log2) – – – -0.07 0.09 -0.08 -0.1 -0.07 -0.17 -0.14 -0.11 -0.25 0.51 0.49 0.44 0.54 0.46 0.49 0.69 0.57 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4867 1.9392 2.1278 2.0632 1.7707 2.1425 0.621 -0.0687 -0.0153 0.1885 0.7961 -0.0743 -0.6751 -0.9491 -1.1011 -0.6713 -0.6273 -0.6319
Actin 0.022 0.0039 0.0095 0.002 0.008 0.0041 0.0446 0.0026 0.0029 0.0122 0.0428 0.006 0.0737 0.0046 0.0479 0.011 0.0516 0.0002
Bud 0.0042 0.0098 0.0144 0.0146 0.0344 0.0059 0.0024 0.0043 0.0017 0.004 0.0021 0.001 0.0009 0.0006 0.0017 0.0003 0.0003 0
Bud Neck 0.0131 0.0005 0.0008 0.0059 0.0028 0.0008 0.0032 0.0003 0.0013 0.0021 0.001 0.0009 0.0016 0.0002 0.0071 0.0188 0.001 0.0001
Bud Periphery 0.0062 0.0059 0.011 0.0181 0.0368 0.01 0.0096 0.0054 0.0027 0.0095 0.0042 0.0023 0.0012 0.0003 0.0028 0.0003 0.0004 0.0001
Bud Site 0.0338 0.0218 0.0175 0.0118 0.0076 0.0007 0.0113 0.0135 0.0082 0.0225 0.0057 0.0006 0.0104 0.0063 0.0075 0.0038 0.0007 0
Cell Periphery 0.0012 0.0006 0.0006 0.0006 0.0004 0.0002 0.0006 0.0005 0.0005 0.001 0.0004 0.0002 0.0003 0.0002 0.0003 0.0002 0.0001 0
Cytoplasm 0.1207 0.1734 0.1231 0.1665 0.1018 0.1694 0.1726 0.3138 0.3946 0.3461 0.3096 0.479 0.119 0.1837 0.1818 0.2796 0.1862 0.3705
Cytoplasmic Foci 0.0363 0.0155 0.0195 0.0205 0.063 0.0258 0.055 0.0079 0.0225 0.0268 0.025 0.0113 0.0277 0.0105 0.0156 0.0219 0.045 0.0131
Eisosomes 0.0005 0.0003 0.0003 0.0004 0.0002 0.0001 0.0005 0.0006 0.0001 0.0003 0.0004 0.0001 0.0004 0.0002 0.0004 0.0003 0.0002 0
Endoplasmic Reticulum 0.0064 0.0018 0.0025 0.0017 0.0014 0.0016 0.0079 0.0037 0.0023 0.0132 0.0027 0.0026 0.0038 0.0017 0.0025 0.0016 0.0022 0.0032
Endosome 0.0261 0.0039 0.0039 0.0102 0.0139 0.0127 0.0594 0.0035 0.0165 0.0301 0.115 0.0135 0.0162 0.0022 0.0065 0.0073 0.0285 0.0057
Golgi 0.0102 0.0006 0.0018 0.0015 0.0152 0.0022 0.0203 0.0006 0.002 0.0187 0.0152 0.0047 0.0034 0.0005 0.0045 0.0078 0.0171 0.0002
Lipid Particles 0.0171 0.0007 0.0053 0.0037 0.0217 0.0015 0.0278 0.0008 0.001 0.0086 0.0191 0.001 0.0077 0.0017 0.0035 0.0217 0.0286 0.0002
Mitochondria 0.0125 0.001 0.0014 0.0052 0.012 0.0018 0.0196 0.0007 0.001 0.0216 0.0219 0.0085 0.0018 0.0006 0.0031 0.0012 0.0041 0.0008
None 0.6126 0.7369 0.7556 0.6798 0.5877 0.7487 0.4702 0.6342 0.5184 0.4568 0.3932 0.4612 0.6263 0.7836 0.7023 0.6131 0.5464 0.565
Nuclear Periphery 0.0122 0.0012 0.0014 0.0046 0.003 0.0024 0.0315 0.001 0.0014 0.003 0.0232 0.0016 0.0236 0.0004 0.0016 0.0007 0.0047 0.0292
Nucleolus 0.0056 0.0011 0.0029 0.0058 0.0057 0.0006 0.0039 0.0005 0.0004 0.0015 0.0004 0.0002 0.0039 0.0002 0.0011 0.0002 0.0004 0.0002
Nucleus 0.0175 0.0053 0.0049 0.0135 0.0176 0.0026 0.0086 0.0018 0.0024 0.0028 0.0023 0.0015 0.0558 0.0005 0.003 0.0003 0.0011 0.0059
Peroxisomes 0.0153 0.0012 0.0116 0.0138 0.0235 0.002 0.0144 0.0004 0.0039 0.0029 0.0035 0.0005 0.0051 0.0004 0.003 0.0071 0.0636 0.0001
Punctate Nuclear 0.0119 0.0125 0.0101 0.0153 0.038 0.0044 0.0243 0.0016 0.0054 0.0029 0.0035 0.0012 0.0137 0.0011 0.0018 0.0012 0.0166 0.0012
Vacuole 0.0114 0.0018 0.0017 0.0035 0.0045 0.0017 0.0087 0.0021 0.0093 0.0101 0.0041 0.0017 0.0025 0.0007 0.0014 0.0012 0.0006 0.0016
Vacuole Periphery 0.0032 0.0002 0.0003 0.001 0.0008 0.0007 0.0037 0.0003 0.0014 0.0036 0.0044 0.0007 0.0009 0.0001 0.0005 0.0002 0.0005 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.8969 13.6641 8.2382 11.1576 13.6249 10.5028 10.3618 13.4042 9.7348 14.4244
Translational Efficiency 0.5885 0.4147 0.5144 0.4435 0.4195 0.6766 0.5157 0.3878 0.3577 0.3332

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
953 369 1238 668 987 1023 529 617 1940 1392 1767 1285

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 674.85 713.70 845.39 844.08 697.36 719.74 807.96 846.73 686.30 718.14 834.18 845.35
Standard Deviation 87.76 104.10 96.32 111.66 83.97 92.17 98.80 102.53 86.59 95.52 98.57 107.38
Intensity Change Log 2 — 0.080751 0.325050 0.322813 — 0.045572 0.212380 0.279998 — 0.062980 0.268891 0.301213

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000697 0.001058 0.000275 0.004309 0.000141 0.000469 0.000095 0.000601 0.000414 0.000625 0.000221 0.002529
Bud Neck 0.026005 0.032804 0.003568 0.009420 0.011001 0.031429 0.002577 0.005173 0.018372 0.031794 0.003271 0.007381
Bud Site 0.019027 0.023036 0.004877 0.052261 0.006596 0.020988 0.006727 0.034859 0.012702 0.021531 0.005431 0.043905
Cell Periphery 0.000187 0.000181 0.000159 0.000225 0.000178 0.000284 0.000375 0.000199 0.000182 0.000257 0.000224 0.000213
Cytoplasm 0.504580* 0.491620* 0.500827* 0.398248* 0.551491* 0.482070* 0.414615* 0.516258* 0.528447* 0.484602* 0.475018* 0.454911*
Cytoplasmic Foci 0.245099* 0.224817* 0.018926 0.045955 0.212627* 0.210972* 0.043383 0.049511 0.228578* 0.214642* 0.026248 0.047662
Eisosomes 0.000215 0.000110 0.000045 0.000087 0.000214 0.000178 0.000132 0.000064 0.000214 0.000160 0.000071 0.000076
Endoplasmic Reticulum 0.000596 0.000995 0.002544 0.002913 0.000636 0.000472 0.000789 0.002011 0.000616 0.000611 0.002019 0.002480
Endosome 0.015458 0.026413 0.002818 0.017638 0.008653 0.021018 0.004413 0.010443 0.011996 0.022448 0.003295 0.014183
Golgi 0.004076 0.005394 0.000795 0.015934 0.003208 0.005038 0.002720 0.008123 0.003634 0.005132 0.001371 0.012183
Lipid Particles 0.006690 0.002969 0.001820 0.003432 0.004925 0.005070 0.002207 0.001632 0.005792 0.004513 0.001936 0.002568
Mitochondria 0.007329 0.005283 0.000523 0.014143 0.002877 0.008253 0.001247 0.001404 0.005064 0.007466 0.000740 0.008026
Mitotic Spindle 0.002017 0.007221 0.003617 0.047245 0.001297 0.005230 0.001892 0.015129 0.001650 0.005758 0.003101 0.031824
None 0.003342 0.003183 0.003713 0.001814 0.004507 0.003128 0.010791 0.004048 0.003935 0.003143 0.005832 0.002887
Nuclear Periphery 0.000271 0.000254 0.001435 0.001904 0.000246 0.000296 0.000763 0.000913 0.000258 0.000285 0.001234 0.001428
Nuclear Periphery Foci 0.000338 0.000581 0.001288 0.003696 0.000490 0.000295 0.000446 0.001666 0.000415 0.000371 0.001036 0.002721
Nucleolus 0.000921 0.001276 0.000439 0.000927 0.000602 0.001253 0.000901 0.000358 0.000759 0.001259 0.000577 0.000653
Nucleus 0.084693 0.062366 0.380080* 0.109553 0.117400 0.088205 0.445427* 0.180314* 0.101333 0.081356 0.399643* 0.143530*
Peroxisomes 0.011136 0.023600 0.000253 0.006235 0.003228 0.010247 0.000708 0.003172 0.007113 0.013787 0.000389 0.004764
Vacuole 0.066400 0.085679 0.071688 0.256395* 0.069169 0.102452 0.059031 0.163000 0.067809 0.098006 0.067899 0.211551*
Vacuole Periphery 0.000923 0.001160 0.000310 0.007667 0.000515 0.002651 0.000758 0.001122 0.000715 0.002256 0.000444 0.004524

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.64 0.98 -1.12 -0.75 -1.39 -3.67 0.90 -3.70 -1.03 -3.95 -1.10 0.52 -1.55 -1.18 -1.70
Bud Neck -1.05 8.54 6.60 4.71 -3.34 -6.76 6.28 3.12 8.18 -2.34 -4.61 10.41 7.21 9.44 -3.83
Bud Site -0.92 5.65 -2.19 -1.26 -6.35 -4.99 0.12 -3.56 0.03 -3.33 -3.49 4.64 -4.08 -1.12 -7.16
Cell Periphery 0.15 1.71 0.97 0.22 -0.53 -0.74 -3.25 1.56 1.02 3.81 -0.69 -1.51 1.77 0.98 2.79
Cytoplasm 1.02 6.08 13.26 8.85 8.37 5.76 10.99 10.88 5.45 -0.32 4.61 12.02 17.19 11.05 6.06
Cytoplasmic Foci 1.59 32.44 28.77 16.54 -8.34 0.39 26.48 26.20 23.81 0.99 2.15 43.48 38.93 29.82 -6.04
Eisosomes 4.78 8.55 6.44 1.57 -8.02 0.91 4.05 7.20 2.25 4.75 1.78 10.29 9.62 2.33 -2.32
Endoplasmic Reticulum -0.82 -13.60 -11.43 -4.61 1.39 0.88 -2.79 -9.24 -11.81 -7.92 -0.10 -13.45 -14.59 -12.83 -0.73
Endosome -2.99 9.02 3.09 4.63 -5.10 -6.31 3.59 1.85 7.12 -1.32 -5.81 10.32 3.47 8.00 -5.27
Golgi -0.58 4.01 -0.71 -0.21 -2.38 -1.33 0.58 0.36 1.41 -0.19 -1.31 3.43 -0.43 0.48 -2.34
Lipid Particles 3.66 5.26 3.63 -0.23 -3.54 -0.14 5.35 6.17 4.40 1.36 1.84 7.32 5.88 3.51 -2.81
Mitochondria 0.92 3.42 0.79 -0.10 -2.95 -2.81 2.05 2.89 4.34 0.96 -1.32 4.13 1.46 2.69 -2.78
Mitotic Spindle -1.44 -1.31 -6.16 -4.44 -5.75 -2.35 -0.89 -4.59 -3.19 -4.17 -2.62 -1.77 -7.64 -5.87 -6.93
None 0.16 -0.24 1.80 0.89 2.84 1.13 -2.49 0.50 -1.01 3.06 0.96 -1.71 1.39 0.06 3.53
Nuclear Periphery 0.01 -21.06 -6.44 -6.43 -0.50 -1.38 -11.95 -15.29 -14.63 -7.49 -1.03 -24.62 -10.79 -10.56 -1.74
Nuclear Periphery Foci -1.47 -9.11 -7.83 -4.96 -4.50 2.13 -0.63 -7.83 -8.72 -7.65 -0.25 -8.04 -10.89 -10.22 -7.19
Nucleolus -1.46 2.00 -0.62 0.42 -1.65 -3.19 -2.15 -1.07 1.80 0.42 -2.73 1.61 -0.91 0.88 -1.74
Nucleus 3.22 -37.57 -6.30 -8.15 27.89 4.95 -25.46 -8.70 -12.13 17.35 4.42 -44.56 -10.36 -13.27 31.42
Peroxisomes -2.47 7.05 4.07 4.13 -2.37 -5.31 5.27 1.07 5.50 -2.33 -3.72 8.46 4.07 6.32 -3.34
Vacuole -3.15 -6.39 -26.44 -22.08 -22.68 -5.79 -1.69 -18.60 -14.98 -16.03 -6.94 -6.44 -31.69 -26.12 -26.86
Vacuole Periphery -1.04 4.37 -2.36 -2.09 -3.23 -3.05 -0.57 0.43 3.08 0.72 -2.94 2.78 -2.26 0.78 -3.02
Standard name
Human Ortholog
Description Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; forms actin-like short filament composed of 9 or 10 Arp1p monomers; putative ortholog of mammalian centractin
Localization
Cell Percentages cytoplasm (39%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Arp1

Arp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available