Standard name
Human Ortholog
Description Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; forms foci at the nuclear periphery upon DNA replication stress; relocalizes to the cytosol in response to hypoxia; homolog of mammalian INSIG proteins; NSG1 has a paralog, NSG2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0.12 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0.07 0 0 0 0.07 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.08 0.14 0.18 0.29 0.2 0.24 0.24 0.3 0.34 0.08 0 0 0 0.07 0.05 0 0 0 0 0 0
Nucleus 0.21 0.06 0.06 0.06 0.08 0.28 0.21 0.23 0.26 0.26 0.32 0.3 0 0.06 0.06 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0.78 0.58 0.81 0.76 0.71 0.58 0.61 0.53 0.6 0.55 0.49 0.5 0.66 0.57 0.41 0.63 0.64 0.65 0.83 0.78 0.86 0.74 0.56 0.47
Nucleolus 0.14 0 0.07 0 0.07 0.08 0.12 0.15 0.13 0.12 0.11 0.13 0.15 0.15 0.18 0.13 0.1 0.05 0 0 0 0 0.05 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.52 0.26 0.25 0.23 0.18 0.13 0.14 0.11 0.13 0.11 0.08 0.31 0.34 0.33 0.37 0.31 0.48 0.05 0.13 0 0.11 0.21 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 4 5 0 0 0 0 1 1 0 0 0
Bud 0 0 0 0 0 0 1 1 3 5 0 7 0 1 0 1 0 0 1 2 2 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 2 2 5 9 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 4 7 0 0 0 0 0 0 0 0 0
Cytoplasm 6 8 1 3 7 8 7 11 5 7 2 6 1 6 9 5 5 1 2 0 1 0 0 1
Endoplasmic Reticulum 2 0 3 0 0 0 3 2 1 3 0 1 4 6 4 1 0 3 1 8 15 5 4 1
Endosome 1 2 0 0 3 1 0 3 2 7 1 2 1 5 8 1 0 1 1 0 1 0 2 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 2 1
Mitochondria 2 2 29 24 49 64 162 125 127 169 138 198 5 1 2 3 3 2 1 2 6 1 1 2
Nucleus 25 18 22 18 27 100 115 142 137 183 146 172 1 7 9 6 1 1 5 6 3 0 1 4
Nuclear Periphery 93 167 298 239 241 207 340 330 310 394 223 292 41 71 58 67 27 26 120 244 330 52 52 71
Nucleolus 17 7 27 13 25 29 65 97 68 88 52 76 9 18 26 14 4 2 1 1 0 1 4 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 1 2 1 1 1 1 3 1 8 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 6 149 96 80 79 63 72 85 59 94 50 44 19 42 46 39 13 19 7 41 14 7 19 50
Unique Cell Count 119 286 366 315 339 359 558 626 519 716 458 581 62 124 141 106 42 40 146 315 383 71 95 151
Labelled Cell Count 152 353 476 378 431 472 768 800 715 957 622 804 85 166 184 138 53 55 146 315 383 71 95 151


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.3 16.0 10.1 9.9 8.1 7.2 6.2 6.6 6.4 6.1 6.1 5.8 13.4 12.8 12.9 13.0 13.0 14.6 15.8 15.5 15.6
Std Deviation (1e-4) 3.5 3.6 2.3 2.3 1.8 1.6 1.9 1.8 1.7 1.7 1.6 1.5 2.4 3.0 3.0 3.0 4.0 3.5 3.5 3.5 3.8
Intensity Change (Log2) – – – -0.03 -0.32 -0.48 -0.69 -0.61 -0.64 -0.72 -0.72 -0.79 0.42 0.35 0.36 0.37 0.37 0.54 0.65 0.62 0.64

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.1 2.8 4.0 7.7 5.1 6.4 6.3 7.9 9.2 0 -2.9 -2.7 -1.8 0 0
Nucleus -0.2 1.0 7.9 6.1 6.8 7.8 7.8 9.2 8.8 0 -0.1 0.2 -0.1 0 0
Nuclear Periphery -1.8 -3.2 -7.0 -6.6 -9.1 -6.9 -8.6 -9.7 -9.6 -2.7 -5.4 -8.9 -3.9 -2.6 -2.5
Nucleolus -1.8 0 0.4 2.1 3.7 2.7 2.5 1.9 2.7 1.9 2.4 3.6 1.9 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.2 -0.9 -2.8 -5.1 -5.0 -5.7 -5.4 -5.7 -7.9 0.7 1.6 1.4 2.1 0.7 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6848 7.4743 7.3849 7.2742 5.314 7.197 11.8827 10.7342 11.1634 10.4798 10.4371 10.2356 9.5929 8.1724 7.7826 7.5914 7.5362 7.3869
Actin 0.0066 0.0002 0.0025 0.0017 0.0054 0.0008 0.0021 0.0001 0.0046 0.0003 0.0029 0.0008 0.0009 0.0001 0.0002 0.0002 0.0001 0.0001
Bud 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Bud Neck 0.0053 0.0001 0.0011 0.0001 0.0003 0.0001 0 0 0 0 0.0001 0 0 0 0 0 0 0
Bud Periphery 0.0002 0 0.0002 0.0002 0.0001 0 0 0 0.0003 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0
Bud Site 0.0071 0.0002 0.0013 0.0011 0.0004 0 0 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Cell Periphery 0.0002 0 0.0003 0.0002 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0 0 0
Cytoplasm 0.002 0.0011 0.0016 0.0047 0.001 0.0004 0.0002 0.0001 0 0 0.0008 0.0001 0.0021 0.0001 0 0.0001 0 0
Cytoplasmic Foci 0.0171 0.0031 0.0091 0.0072 0.0297 0.0022 0.0121 0 0.0019 0 0.0042 0.0003 0.0004 0 0 0 0 0
Eisosomes 0.0001 0 0.0001 0.0001 0.0001 0.0002 0 0 0.0001 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0045 0.0068 0.0063 0.0091 0.0145 0.0016 0.0013 0.0039 0.0059 0.0058 0.0033 0.0009 0.0014 0.0026 0.0026 0.0021 0.0035 0.0005
Endosome 0.0286 0.0026 0.0094 0.0131 0.0491 0.0027 0.0036 0.0002 0.0011 0.0004 0.0117 0.0013 0.0008 0.0001 0.0002 0.0024 0.0003 0.0001
Golgi 0.0089 0.0004 0.0058 0.0025 0.0214 0.0007 0.0008 0 0.0009 0.0001 0.0022 0.0008 0.0001 0 0 0 0.0001 0
Lipid Particles 0.0293 0.0003 0.0193 0.0053 0.0498 0.0061 0.0067 0.0002 0.004 0.0002 0.0254 0.0024 0.0003 0.0002 0.0002 0.0001 0.0003 0.0001
Mitochondria 0.0028 0.0004 0.0137 0.0012 0.0114 0.0052 0.0008 0.0004 0.0028 0.0012 0.0018 0.0007 0.0009 0.0004 0.001 0.0005 0.0005 0.0002
None 0.0041 0.0001 0.0007 0.0012 0.0027 0.0037 0.0006 0 0.0001 0.0001 0.0008 0.0001 0.0041 0 0.0001 0.0001 0 0
Nuclear Periphery 0.8084 0.862 0.8221 0.8641 0.6923 0.9245 0.9218 0.9304 0.9046 0.9383 0.8826 0.9738 0.9415 0.9335 0.9305 0.889 0.9563 0.9673
Nucleolus 0.002 0.0007 0.0008 0.0007 0.0005 0.0007 0.0001 0.0001 0.0001 0.0001 0.0013 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0245 0.0774 0.0472 0.043 0.0032 0.0268 0.0052 0.0081 0.0046 0.0029 0.0065 0.0014 0.0081 0.0136 0.0052 0.0284 0.0029 0.0134
Peroxisomes 0.0073 0.0002 0.0101 0.0015 0.0554 0.0004 0.0069 0 0.0043 0 0.0006 0.0005 0.0001 0 0 0 0 0
Punctate Nuclear 0.0084 0.0046 0.0014 0.0032 0.0192 0.0062 0.0011 0.0001 0.0003 0.0001 0.0095 0.0002 0.0015 0.0001 0.0001 0.0001 0.0002 0.0001
Vacuole 0.0031 0.0027 0.0043 0.0036 0.0084 0.0008 0.0006 0.0009 0.0014 0.0006 0.0016 0.0001 0.0005 0.0005 0.0007 0.0118 0.0004 0.0002
Vacuole Periphery 0.0294 0.0372 0.0426 0.0362 0.035 0.0168 0.036 0.0555 0.0629 0.0498 0.0443 0.0166 0.0369 0.0486 0.0587 0.0649 0.035 0.0178

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 85.0226 61.93 77.7053 80.5158 89.8215 56.6504 77.1332 69.9252 71.7975 87.2571
Translational Efficiency 1.2791 1.5372 1.1164 1.0232 1.0749 1.7132 1.1308 1.1504 1.0499 1.1705

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1444 1438 458 1182 1833 1828 217 1928 3277 3266 675 3110

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1212.81 1240.25 1618.40 1521.95 1109.12 1218.57 1701.10 1588.60 1154.81 1228.12 1644.99 1563.27
Standard Deviation 223.19 313.82 267.53 340.79 210.31 221.69 249.66 325.22 222.12 266.43 264.75 332.80
Intensity Change Log 2 — 0.032277 0.416215 0.327567 — 0.135774 0.617052 0.518340 — 0.082642 0.515641 0.421845

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000144 0.000801 0.000464 0.000368 0.000476 0.000339 0.000137 0.000358 0.000330 0.000543 0.000359 0.000362
Bud Neck 0.000172 0.000853 0.002208 0.004884 0.000504 0.000381 0.001957 0.002952 0.000358 0.000589 0.002127 0.003686
Bud Site 0.000059 0.000803 0.000130 0.001116 0.000389 0.000238 0.000067 0.001144 0.000243 0.000487 0.000110 0.001133
Cell Periphery 0.000119 0.000252 0.000032 0.000193 0.000196 0.000122 0.000020 0.000194 0.000162 0.000179 0.000028 0.000193
Cytoplasm 0.001138 0.004037 0.000054 0.000664 0.001751 0.000707 0.000039 0.001097 0.001481 0.002173 0.000049 0.000932
Cytoplasmic Foci 0.000139 0.000755 0.000655 0.004464 0.000711 0.000552 0.000276 0.002727 0.000459 0.000641 0.000533 0.003387
Eisosomes 0.000002 0.000008 0.000013 0.000016 0.000006 0.000004 0.000003 0.000009 0.000004 0.000006 0.000010 0.000012
Endoplasmic Reticulum 0.030065 0.029177 0.001464 0.007128 0.063069 0.037444 0.005866 0.008745 0.048526 0.033804 0.002879 0.008131
Endosome 0.002947 0.005298 0.000563 0.008033 0.006724 0.004389 0.001364 0.006799 0.005060 0.004789 0.000820 0.007268
Golgi 0.000077 0.000550 0.000241 0.002442 0.000636 0.000493 0.000153 0.001156 0.000390 0.000518 0.000213 0.001645
Lipid Particles 0.001029 0.003436 0.000342 0.015072 0.005257 0.003452 0.001481 0.009619 0.003394 0.003445 0.000708 0.011692
Mitochondria 0.001911 0.004554 0.001734 0.004426 0.004814 0.003166 0.000706 0.003327 0.003535 0.003777 0.001403 0.003744
Mitotic Spindle 0.000036 0.001395 0.009453 0.027989 0.000583 0.001204 0.001463 0.015799 0.000341 0.001288 0.006885 0.020432
None 0.001408 0.007323 0.000131 0.000917 0.001735 0.001297 0.000028 0.001038 0.001591 0.003950 0.000098 0.000992
Nuclear Periphery 0.733114 0.662830 0.718746 0.417168 0.614757 0.690207 0.798396 0.425741 0.666911 0.678153 0.744352 0.422483
Nuclear Periphery Foci 0.204911 0.217479 0.173950 0.439816 0.271335 0.235767 0.178486 0.456849 0.242066 0.227715 0.175408 0.450376
Nucleolus 0.001979 0.015486 0.061471 0.022126 0.003992 0.003320 0.003122 0.018542 0.003105 0.008676 0.042713 0.019904
Nucleus 0.003434 0.032175 0.021654 0.006713 0.007153 0.005727 0.002303 0.009815 0.005514 0.017372 0.015433 0.008636
Peroxisomes 0.000086 0.000315 0.001078 0.000559 0.000335 0.000345 0.000125 0.000396 0.000225 0.000332 0.000772 0.000458
Vacuole 0.007855 0.007257 0.001514 0.015899 0.007884 0.005642 0.001778 0.015238 0.007871 0.006353 0.001599 0.015489
Vacuole Periphery 0.009375 0.005216 0.004104 0.020008 0.007694 0.005204 0.002229 0.018456 0.008434 0.005209 0.003501 0.019046

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.04 -4.97 -4.40 5.15 1.04 2.17 5.50 1.27 -0.48 -3.56 -4.96 -0.55 -0.99 3.22 -0.33
Bud Neck -6.64 -6.26 -9.52 -8.05 -4.77 0.94 -1.40 -8.94 -9.06 -1.19 -3.25 -4.41 -13.13 -11.97 -3.79
Bud Site -2.23 -3.21 -4.05 -1.02 -3.81 1.00 3.43 -2.36 -2.63 -3.10 -1.61 2.49 -3.83 -2.60 -4.30
Cell Periphery -3.22 3.79 -2.14 1.05 -5.29 2.28 6.96 -0.16 -2.09 -5.60 -0.73 7.71 -1.40 -0.74 -7.39
Cytoplasm -3.59 3.87 -0.09 2.95 -1.83 2.10 3.83 0.97 -1.57 -4.55 -1.66 5.12 0.63 2.18 -4.09
Cytoplasmic Foci -3.69 -4.16 -6.07 -5.19 -5.39 0.69 3.14 -3.68 -3.87 -4.47 -1.37 -0.65 -6.71 -6.22 -6.58
Eisosomes -8.91 -4.20 -7.75 -4.21 -1.16 1.44 2.76 -3.43 -4.64 -5.78 -3.25 -3.08 -8.46 -6.02 -1.19
Endoplasmic Reticulum 0.27 11.90 8.74 9.19 -5.48 6.38 15.54 15.87 11.07 -1.41 5.50 20.79 17.87 14.23 -5.48
Endosome -3.08 5.08 -5.45 -2.80 -8.85 3.52 6.71 -0.18 -3.14 -6.06 0.52 10.37 -3.70 -4.09 -11.57
Golgi -4.20 -5.05 -4.47 -3.51 -4.16 0.45 2.82 -2.38 -3.04 -6.92 -1.10 1.82 -5.26 -4.62 -6.51
Lipid Particles -3.46 5.37 -8.43 -6.49 -8.86 2.71 4.83 -3.26 -4.90 -6.16 -0.09 7.29 -8.05 -7.91 -11.04
Mitochondria -4.61 0.42 -2.59 0.09 -2.69 2.09 5.66 1.64 -0.44 -5.30 -0.49 4.52 -0.47 -0.08 -4.61
Mitotic Spindle -1.86 -3.41 -8.52 -7.92 -3.99 -1.25 -1.37 -7.90 -7.44 -6.99 -2.23 -3.46 -11.62 -10.83 -4.92
None -3.79 4.36 0.94 4.02 -1.90 1.03 6.60 1.88 0.61 -4.13 -3.25 7.69 1.97 4.00 -4.10
Nuclear Periphery 7.40 1.27 33.68 23.25 21.54 -8.87 -13.88 22.02 32.29 28.61 -1.65 -7.54 38.16 39.20 31.81
Nuclear Periphery Foci -1.81 3.68 -26.99 -25.35 -26.69 5.42 8.19 -23.69 -29.30 -23.25 2.99 9.97 -35.58 -38.86 -37.27
Nucleolus -8.51 -8.55 -7.40 -2.23 5.26 0.60 1.06 -7.50 -7.54 -7.70 -6.78 -8.18 -10.60 -6.49 4.48
Nucleus -9.34 -7.18 -3.11 8.09 5.70 0.90 4.15 -2.00 -2.83 -6.01 -7.57 -5.48 -3.38 5.17 3.50
Peroxisomes -7.86 -7.66 -5.52 -2.82 3.17 -0.44 4.37 -0.97 -0.12 -2.63 -1.26 -5.92 -3.23 -0.86 2.16
Vacuole 0.46 6.45 -4.72 -5.43 -9.82 2.27 7.33 -5.14 -6.83 -10.02 1.95 10.12 -6.91 -8.62 -14.02
Vacuole Periphery 3.76 4.73 -4.98 -7.72 -8.27 3.25 8.57 -6.67 -8.31 -10.53 4.97 7.76 -8.17 -11.30 -12.75
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; forms foci at the nuclear periphery upon DNA replication stress; relocalizes to the cytosol in response to hypoxia; homolog of mammalian INSIG proteins; NSG1 has a paralog, NSG2, that arose from the whole genome duplication
Localization
Cell Percentages nuclear periphery (34%), ER (8%), mixed (56%)
Cell Cycle Regulation Yes
nuclear periphery - T/G1 (0.00012)
ER - MA (3.8e-05)
Subcompartmental Group N/A

Nsg1

Nsg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nsg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available