Standard name | |
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Human Ortholog | |
Description | Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; forms foci at the nuclear periphery upon DNA replication stress; relocalizes to the cytosol in response to hypoxia; homolog of mammalian INSIG proteins; NSG1 has a paralog, NSG2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 5 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 5 | 0 | 7 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 5 | 9 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 8 | 1 | 3 | 7 | 8 | 7 | 11 | 5 | 7 | 2 | 6 | 1 | 6 | 9 | 5 | 5 | 1 | 2 | 0 | 1 | 0 | 0 | 1 |
Endoplasmic Reticulum | 2 | 0 | 3 | 0 | 0 | 0 | 3 | 2 | 1 | 3 | 0 | 1 | 4 | 6 | 4 | 1 | 0 | 3 | 1 | 8 | 15 | 5 | 4 | 1 |
Endosome | 1 | 2 | 0 | 0 | 3 | 1 | 0 | 3 | 2 | 7 | 1 | 2 | 1 | 5 | 8 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 |
Mitochondria | 2 | 2 | 29 | 24 | 49 | 64 | 162 | 125 | 127 | 169 | 138 | 198 | 5 | 1 | 2 | 3 | 3 | 2 | 1 | 2 | 6 | 1 | 1 | 2 |
Nucleus | 25 | 18 | 22 | 18 | 27 | 100 | 115 | 142 | 137 | 183 | 146 | 172 | 1 | 7 | 9 | 6 | 1 | 1 | 5 | 6 | 3 | 0 | 1 | 4 |
Nuclear Periphery | 93 | 167 | 298 | 239 | 241 | 207 | 340 | 330 | 310 | 394 | 223 | 292 | 41 | 71 | 58 | 67 | 27 | 26 | 120 | 244 | 330 | 52 | 52 | 71 |
Nucleolus | 17 | 7 | 27 | 13 | 25 | 29 | 65 | 97 | 68 | 88 | 52 | 76 | 9 | 18 | 26 | 14 | 4 | 2 | 1 | 1 | 0 | 1 | 4 | 5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 1 | 3 | 1 | 8 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 6 | 149 | 96 | 80 | 79 | 63 | 72 | 85 | 59 | 94 | 50 | 44 | 19 | 42 | 46 | 39 | 13 | 19 | 7 | 41 | 14 | 7 | 19 | 50 |
Unique Cell Count | 119 | 286 | 366 | 315 | 339 | 359 | 558 | 626 | 519 | 716 | 458 | 581 | 62 | 124 | 141 | 106 | 42 | 40 | 146 | 315 | 383 | 71 | 95 | 151 |
Labelled Cell Count | 152 | 353 | 476 | 378 | 431 | 472 | 768 | 800 | 715 | 957 | 622 | 804 | 85 | 166 | 184 | 138 | 53 | 55 | 146 | 315 | 383 | 71 | 95 | 151 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.3 | 16.0 | 10.1 | 9.9 | 8.1 | 7.2 | 6.2 | 6.6 | 6.4 | 6.1 | 6.1 | 5.8 | 13.4 | 12.8 | 12.9 | 13.0 | 13.0 | 14.6 | 15.8 | 15.5 | 15.6 |
Std Deviation (1e-4) | 3.5 | 3.6 | 2.3 | 2.3 | 1.8 | 1.6 | 1.9 | 1.8 | 1.7 | 1.7 | 1.6 | 1.5 | 2.4 | 3.0 | 3.0 | 3.0 | 4.0 | 3.5 | 3.5 | 3.5 | 3.8 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.32 | -0.48 | -0.69 | -0.61 | -0.64 | -0.72 | -0.72 | -0.79 | 0.42 | 0.35 | 0.36 | 0.37 | 0.37 | 0.54 | 0.65 | 0.62 | 0.64 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.1 | 2.8 | 4.0 | 7.7 | 5.1 | 6.4 | 6.3 | 7.9 | 9.2 | 0 | -2.9 | -2.7 | -1.8 | 0 | 0 |
Nucleus | -0.2 | 1.0 | 7.9 | 6.1 | 6.8 | 7.8 | 7.8 | 9.2 | 8.8 | 0 | -0.1 | 0.2 | -0.1 | 0 | 0 |
Nuclear Periphery | -1.8 | -3.2 | -7.0 | -6.6 | -9.1 | -6.9 | -8.6 | -9.7 | -9.6 | -2.7 | -5.4 | -8.9 | -3.9 | -2.6 | -2.5 |
Nucleolus | -1.8 | 0 | 0.4 | 2.1 | 3.7 | 2.7 | 2.5 | 1.9 | 2.7 | 1.9 | 2.4 | 3.6 | 1.9 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.2 | -0.9 | -2.8 | -5.1 | -5.0 | -5.7 | -5.4 | -5.7 | -7.9 | 0.7 | 1.6 | 1.4 | 2.1 | 0.7 | 2.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.6848 | 7.4743 | 7.3849 | 7.2742 | 5.314 | 7.197 | 11.8827 | 10.7342 | 11.1634 | 10.4798 | 10.4371 | 10.2356 | 9.5929 | 8.1724 | 7.7826 | 7.5914 | 7.5362 | 7.3869 |
Actin | 0.0066 | 0.0002 | 0.0025 | 0.0017 | 0.0054 | 0.0008 | 0.0021 | 0.0001 | 0.0046 | 0.0003 | 0.0029 | 0.0008 | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Bud | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0053 | 0.0001 | 0.0011 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Periphery | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0071 | 0.0002 | 0.0013 | 0.0011 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.002 | 0.0011 | 0.0016 | 0.0047 | 0.001 | 0.0004 | 0.0002 | 0.0001 | 0 | 0 | 0.0008 | 0.0001 | 0.0021 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Cytoplasmic Foci | 0.0171 | 0.0031 | 0.0091 | 0.0072 | 0.0297 | 0.0022 | 0.0121 | 0 | 0.0019 | 0 | 0.0042 | 0.0003 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0045 | 0.0068 | 0.0063 | 0.0091 | 0.0145 | 0.0016 | 0.0013 | 0.0039 | 0.0059 | 0.0058 | 0.0033 | 0.0009 | 0.0014 | 0.0026 | 0.0026 | 0.0021 | 0.0035 | 0.0005 |
Endosome | 0.0286 | 0.0026 | 0.0094 | 0.0131 | 0.0491 | 0.0027 | 0.0036 | 0.0002 | 0.0011 | 0.0004 | 0.0117 | 0.0013 | 0.0008 | 0.0001 | 0.0002 | 0.0024 | 0.0003 | 0.0001 |
Golgi | 0.0089 | 0.0004 | 0.0058 | 0.0025 | 0.0214 | 0.0007 | 0.0008 | 0 | 0.0009 | 0.0001 | 0.0022 | 0.0008 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Lipid Particles | 0.0293 | 0.0003 | 0.0193 | 0.0053 | 0.0498 | 0.0061 | 0.0067 | 0.0002 | 0.004 | 0.0002 | 0.0254 | 0.0024 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 |
Mitochondria | 0.0028 | 0.0004 | 0.0137 | 0.0012 | 0.0114 | 0.0052 | 0.0008 | 0.0004 | 0.0028 | 0.0012 | 0.0018 | 0.0007 | 0.0009 | 0.0004 | 0.001 | 0.0005 | 0.0005 | 0.0002 |
None | 0.0041 | 0.0001 | 0.0007 | 0.0012 | 0.0027 | 0.0037 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0041 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.8084 | 0.862 | 0.8221 | 0.8641 | 0.6923 | 0.9245 | 0.9218 | 0.9304 | 0.9046 | 0.9383 | 0.8826 | 0.9738 | 0.9415 | 0.9335 | 0.9305 | 0.889 | 0.9563 | 0.9673 |
Nucleolus | 0.002 | 0.0007 | 0.0008 | 0.0007 | 0.0005 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0245 | 0.0774 | 0.0472 | 0.043 | 0.0032 | 0.0268 | 0.0052 | 0.0081 | 0.0046 | 0.0029 | 0.0065 | 0.0014 | 0.0081 | 0.0136 | 0.0052 | 0.0284 | 0.0029 | 0.0134 |
Peroxisomes | 0.0073 | 0.0002 | 0.0101 | 0.0015 | 0.0554 | 0.0004 | 0.0069 | 0 | 0.0043 | 0 | 0.0006 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0084 | 0.0046 | 0.0014 | 0.0032 | 0.0192 | 0.0062 | 0.0011 | 0.0001 | 0.0003 | 0.0001 | 0.0095 | 0.0002 | 0.0015 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Vacuole | 0.0031 | 0.0027 | 0.0043 | 0.0036 | 0.0084 | 0.0008 | 0.0006 | 0.0009 | 0.0014 | 0.0006 | 0.0016 | 0.0001 | 0.0005 | 0.0005 | 0.0007 | 0.0118 | 0.0004 | 0.0002 |
Vacuole Periphery | 0.0294 | 0.0372 | 0.0426 | 0.0362 | 0.035 | 0.0168 | 0.036 | 0.0555 | 0.0629 | 0.0498 | 0.0443 | 0.0166 | 0.0369 | 0.0486 | 0.0587 | 0.0649 | 0.035 | 0.0178 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 85.0226 | 61.93 | 77.7053 | 80.5158 | 89.8215 | 56.6504 | 77.1332 | 69.9252 | 71.7975 | 87.2571 |
Translational Efficiency | 1.2791 | 1.5372 | 1.1164 | 1.0232 | 1.0749 | 1.7132 | 1.1308 | 1.1504 | 1.0499 | 1.1705 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1444 | 1438 | 458 | 1182 | 1833 | 1828 | 217 | 1928 | 3277 | 3266 | 675 | 3110 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1212.81 | 1240.25 | 1618.40 | 1521.95 | 1109.12 | 1218.57 | 1701.10 | 1588.60 | 1154.81 | 1228.12 | 1644.99 | 1563.27 |
Standard Deviation | 223.19 | 313.82 | 267.53 | 340.79 | 210.31 | 221.69 | 249.66 | 325.22 | 222.12 | 266.43 | 264.75 | 332.80 |
Intensity Change Log 2 | — | 0.032277 | 0.416215 | 0.327567 | — | 0.135774 | 0.617052 | 0.518340 | — | 0.082642 | 0.515641 | 0.421845 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000144 | 0.000801 | 0.000464 | 0.000368 | 0.000476 | 0.000339 | 0.000137 | 0.000358 | 0.000330 | 0.000543 | 0.000359 | 0.000362 |
Bud Neck | 0.000172 | 0.000853 | 0.002208 | 0.004884 | 0.000504 | 0.000381 | 0.001957 | 0.002952 | 0.000358 | 0.000589 | 0.002127 | 0.003686 |
Bud Site | 0.000059 | 0.000803 | 0.000130 | 0.001116 | 0.000389 | 0.000238 | 0.000067 | 0.001144 | 0.000243 | 0.000487 | 0.000110 | 0.001133 |
Cell Periphery | 0.000119 | 0.000252 | 0.000032 | 0.000193 | 0.000196 | 0.000122 | 0.000020 | 0.000194 | 0.000162 | 0.000179 | 0.000028 | 0.000193 |
Cytoplasm | 0.001138 | 0.004037 | 0.000054 | 0.000664 | 0.001751 | 0.000707 | 0.000039 | 0.001097 | 0.001481 | 0.002173 | 0.000049 | 0.000932 |
Cytoplasmic Foci | 0.000139 | 0.000755 | 0.000655 | 0.004464 | 0.000711 | 0.000552 | 0.000276 | 0.002727 | 0.000459 | 0.000641 | 0.000533 | 0.003387 |
Eisosomes | 0.000002 | 0.000008 | 0.000013 | 0.000016 | 0.000006 | 0.000004 | 0.000003 | 0.000009 | 0.000004 | 0.000006 | 0.000010 | 0.000012 |
Endoplasmic Reticulum | 0.030065 | 0.029177 | 0.001464 | 0.007128 | 0.063069 | 0.037444 | 0.005866 | 0.008745 | 0.048526 | 0.033804 | 0.002879 | 0.008131 |
Endosome | 0.002947 | 0.005298 | 0.000563 | 0.008033 | 0.006724 | 0.004389 | 0.001364 | 0.006799 | 0.005060 | 0.004789 | 0.000820 | 0.007268 |
Golgi | 0.000077 | 0.000550 | 0.000241 | 0.002442 | 0.000636 | 0.000493 | 0.000153 | 0.001156 | 0.000390 | 0.000518 | 0.000213 | 0.001645 |
Lipid Particles | 0.001029 | 0.003436 | 0.000342 | 0.015072 | 0.005257 | 0.003452 | 0.001481 | 0.009619 | 0.003394 | 0.003445 | 0.000708 | 0.011692 |
Mitochondria | 0.001911 | 0.004554 | 0.001734 | 0.004426 | 0.004814 | 0.003166 | 0.000706 | 0.003327 | 0.003535 | 0.003777 | 0.001403 | 0.003744 |
Mitotic Spindle | 0.000036 | 0.001395 | 0.009453 | 0.027989 | 0.000583 | 0.001204 | 0.001463 | 0.015799 | 0.000341 | 0.001288 | 0.006885 | 0.020432 |
None | 0.001408 | 0.007323 | 0.000131 | 0.000917 | 0.001735 | 0.001297 | 0.000028 | 0.001038 | 0.001591 | 0.003950 | 0.000098 | 0.000992 |
Nuclear Periphery | 0.733114* | 0.662830* | 0.718746* | 0.417168* | 0.614757* | 0.690207* | 0.798396* | 0.425741* | 0.666911* | 0.678153* | 0.744352* | 0.422483* |
Nuclear Periphery Foci | 0.204911* | 0.217479* | 0.173950 | 0.439816* | 0.271335* | 0.235767* | 0.178486 | 0.456849* | 0.242066* | 0.227715* | 0.175408 | 0.450376* |
Nucleolus | 0.001979 | 0.015486 | 0.061471 | 0.022126 | 0.003992 | 0.003320 | 0.003122 | 0.018542 | 0.003105 | 0.008676 | 0.042713 | 0.019904 |
Nucleus | 0.003434 | 0.032175 | 0.021654 | 0.006713 | 0.007153 | 0.005727 | 0.002303 | 0.009815 | 0.005514 | 0.017372 | 0.015433 | 0.008636 |
Peroxisomes | 0.000086 | 0.000315 | 0.001078 | 0.000559 | 0.000335 | 0.000345 | 0.000125 | 0.000396 | 0.000225 | 0.000332 | 0.000772 | 0.000458 |
Vacuole | 0.007855 | 0.007257 | 0.001514 | 0.015899 | 0.007884 | 0.005642 | 0.001778 | 0.015238 | 0.007871 | 0.006353 | 0.001599 | 0.015489 |
Vacuole Periphery | 0.009375 | 0.005216 | 0.004104 | 0.020008 | 0.007694 | 0.005204 | 0.002229 | 0.018456 | 0.008434 | 0.005209 | 0.003501 | 0.019046 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -10.04 | -4.97 | -4.40 | 5.15 | 1.04 | 2.17 | 5.50 | 1.27 | -0.48 | -3.56 | -4.96 | -0.55 | -0.99 | 3.22 | -0.33 |
Bud Neck | -6.64 | -6.26 | -9.52 | -8.05 | -4.77 | 0.94 | -1.40 | -8.94 | -9.06 | -1.19 | -3.25 | -4.41 | -13.13 | -11.97 | -3.79 |
Bud Site | -2.23 | -3.21 | -4.05 | -1.02 | -3.81 | 1.00 | 3.43 | -2.36 | -2.63 | -3.10 | -1.61 | 2.49 | -3.83 | -2.60 | -4.30 |
Cell Periphery | -3.22 | 3.79 | -2.14 | 1.05 | -5.29 | 2.28 | 6.96 | -0.16 | -2.09 | -5.60 | -0.73 | 7.71 | -1.40 | -0.74 | -7.39 |
Cytoplasm | -3.59 | 3.87 | -0.09 | 2.95 | -1.83 | 2.10 | 3.83 | 0.97 | -1.57 | -4.55 | -1.66 | 5.12 | 0.63 | 2.18 | -4.09 |
Cytoplasmic Foci | -3.69 | -4.16 | -6.07 | -5.19 | -5.39 | 0.69 | 3.14 | -3.68 | -3.87 | -4.47 | -1.37 | -0.65 | -6.71 | -6.22 | -6.58 |
Eisosomes | -8.91 | -4.20 | -7.75 | -4.21 | -1.16 | 1.44 | 2.76 | -3.43 | -4.64 | -5.78 | -3.25 | -3.08 | -8.46 | -6.02 | -1.19 |
Endoplasmic Reticulum | 0.27 | 11.90 | 8.74 | 9.19 | -5.48 | 6.38 | 15.54 | 15.87 | 11.07 | -1.41 | 5.50 | 20.79 | 17.87 | 14.23 | -5.48 |
Endosome | -3.08 | 5.08 | -5.45 | -2.80 | -8.85 | 3.52 | 6.71 | -0.18 | -3.14 | -6.06 | 0.52 | 10.37 | -3.70 | -4.09 | -11.57 |
Golgi | -4.20 | -5.05 | -4.47 | -3.51 | -4.16 | 0.45 | 2.82 | -2.38 | -3.04 | -6.92 | -1.10 | 1.82 | -5.26 | -4.62 | -6.51 |
Lipid Particles | -3.46 | 5.37 | -8.43 | -6.49 | -8.86 | 2.71 | 4.83 | -3.26 | -4.90 | -6.16 | -0.09 | 7.29 | -8.05 | -7.91 | -11.04 |
Mitochondria | -4.61 | 0.42 | -2.59 | 0.09 | -2.69 | 2.09 | 5.66 | 1.64 | -0.44 | -5.30 | -0.49 | 4.52 | -0.47 | -0.08 | -4.61 |
Mitotic Spindle | -1.86 | -3.41 | -8.52 | -7.92 | -3.99 | -1.25 | -1.37 | -7.90 | -7.44 | -6.99 | -2.23 | -3.46 | -11.62 | -10.83 | -4.92 |
None | -3.79 | 4.36 | 0.94 | 4.02 | -1.90 | 1.03 | 6.60 | 1.88 | 0.61 | -4.13 | -3.25 | 7.69 | 1.97 | 4.00 | -4.10 |
Nuclear Periphery | 7.40 | 1.27 | 33.68* | 23.25* | 21.54 | -8.87 | -13.88 | 22.02* | 32.29* | 28.61* | -1.65 | -7.54 | 38.16 | 39.20* | 31.81* |
Nuclear Periphery Foci | -1.81 | 3.68 | -26.99* | -25.35* | -26.69* | 5.42 | 8.19 | -23.69* | -29.30* | -23.25* | 2.99 | 9.97 | -35.58 | -38.86* | -37.27* |
Nucleolus | -8.51 | -8.55 | -7.40 | -2.23 | 5.26 | 0.60 | 1.06 | -7.50 | -7.54 | -7.70 | -6.78 | -8.18 | -10.60 | -6.49 | 4.48 |
Nucleus | -9.34 | -7.18 | -3.11 | 8.09 | 5.70 | 0.90 | 4.15 | -2.00 | -2.83 | -6.01 | -7.57 | -5.48 | -3.38 | 5.17 | 3.50 |
Peroxisomes | -7.86 | -7.66 | -5.52 | -2.82 | 3.17 | -0.44 | 4.37 | -0.97 | -0.12 | -2.63 | -1.26 | -5.92 | -3.23 | -0.86 | 2.16 |
Vacuole | 0.46 | 6.45 | -4.72 | -5.43 | -9.82 | 2.27 | 7.33 | -5.14 | -6.83 | -10.02 | 1.95 | 10.12 | -6.91 | -8.62 | -14.02 |
Vacuole Periphery | 3.76 | 4.73 | -4.98 | -7.72 | -8.27 | 3.25 | 8.57 | -6.67 | -8.31 | -10.53 | 4.97 | 7.76 | -8.17 | -11.30 | -12.75 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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