Standard name | |
---|---|
Human Ortholog | |
Description | Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation by targeting Pho87p to the vacuole; upstream region harbors putative hypoxia response element (HRE) cluster; overproduction suppresses a plc1 null mutation; promoter shows an increase in Snf2p occupancy after heat shock; GFP-fusion protein localizes to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 2 | 1 | 2 | 1 | – | 3 | 1 | 5 | 0 | 4 | 0 | 1 | 1 | 2 | 0 | 1 | 4 | 1 | 3 | 1 | 6 | 4 |
Bud | 2 | 0 | 3 | 4 | 7 | 5 | – | 18 | 18 | 24 | 26 | 40 | 0 | 2 | 1 | 0 | 1 | 2 | 7 | 0 | 2 | 2 | 14 | 11 |
Bud Neck | 0 | 0 | 0 | 2 | 8 | 2 | – | 10 | 5 | 6 | 0 | 5 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | – | 2 | 2 | 4 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 0 | 3 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 191 | 65 | 91 | 286 | 466 | 279 | – | 476 | 343 | 423 | 248 | 465 | 165 | 348 | 447 | 136 | 39 | 93 | 128 | 63 | 57 | 38 | 136 | 158 |
Endoplasmic Reticulum | 3 | 0 | 0 | 2 | 1 | 1 | – | 0 | 0 | 1 | 1 | 2 | 2 | 4 | 5 | 7 | 4 | 9 | 1 | 0 | 0 | 0 | 2 | 2 |
Endosome | 2 | 1 | 1 | 5 | 1 | 1 | – | 13 | 2 | 13 | 6 | 5 | 1 | 2 | 2 | 9 | 2 | 6 | 24 | 3 | 8 | 6 | 21 | 19 |
Golgi | 8 | 2 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 7 | 12 | 2 | 9 | 12 | 0 | 0 | 3 | 15 | 7 |
Mitochondria | 5 | 0 | 4 | 8 | 11 | 55 | – | 74 | 100 | 114 | 121 | 189 | 2 | 4 | 2 | 5 | 2 | 3 | 9 | 4 | 6 | 2 | 9 | 8 |
Nucleus | 0 | 0 | 0 | 1 | 3 | 2 | – | 11 | 2 | 5 | 3 | 4 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | – | 4 | 2 | 4 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 3 | 1 | 0 | 2 | 0 | 1 | – | 6 | 2 | 3 | 6 | 6 | 0 | 2 | 1 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 3 | 1 | 1 | – | 12 | 2 | 7 | 4 | 12 | 1 | 5 | 3 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 4 | 5 |
Vac/Vac Membrane | 13 | 2 | 1 | 10 | 15 | 6 | – | 33 | 19 | 17 | 18 | 22 | 1 | 3 | 6 | 55 | 23 | 32 | 32 | 9 | 4 | 11 | 26 | 31 |
Unique Cell Count | 216 | 68 | 96 | 306 | 491 | 310 | 589 | 416 | 528 | 365 | 626 | 170 | 365 | 468 | 199 | 58 | 134 | 239 | 91 | 89 | 70 | 244 | 255 | |
Labelled Cell Count | 229 | 72 | 102 | 326 | 517 | 355 | 663 | 498 | 629 | 442 | 763 | 175 | 377 | 477 | 229 | 74 | 162 | 239 | 91 | 89 | 70 | 244 | 255 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.5 | 6.9 | 5.5 | 5.5 | 5.3 | 4.1 | – | 4.8 | 4.0 | 4.0 | 4.4 | 4.2 | 9.3 | 11.6 | 8.9 | 18.4 | 21.6 | 20.2 | 11.0 | 9.2 | 7.8 |
Std Deviation (1e-4) | 11.3 | 7.9 | 5.5 | 5.8 | 4.3 | 3.1 | – | 3.5 | 2.4 | 1.9 | 2.5 | 2.3 | 11.5 | 13.1 | 9.7 | 14.9 | 17.0 | 16.5 | 10.2 | 9.5 | 7.6 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.08 | -0.43 | – | -0.21 | -0.46 | -0.47 | -0.34 | -0.42 | 0.74 | 1.07 | 0.69 | 1.73 | 1.96 | 1.87 | 0.98 | 0.74 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | 0 | -1.4 | – | -3.4 | -3.0 | -3.5 | -5.3 | -4.4 | 0.9 | 0.2 | 0.3 | -5.1 | -4.6 | -4.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.5 | 6.4 | 4.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.2616 | -0.6013 | -0.4018 | -0.6185 | -0.1678 | -0.0516 | 0.1141 | -0.2121 | -0.1865 | 0.855 | -1.5438 | 0.4466 | 0.5646 | 2.2462 | 1.589 | 1.7931 | 1.6495 | 1.7445 |
Actin | 0.0245 | 0.0042 | 0.0108 | 0.0322 | 0.0135 | 0.0036 | 0.0385 | 0.003 | 0.0094 | 0.0002 | 0.0017 | 0.0018 | 0.0002 | 0.0001 | 0.0006 | 0.0014 | 0.0007 | 0.0006 |
Bud | 0.001 | 0.0028 | 0.0008 | 0.0097 | 0.0134 | 0.0009 | 0.0013 | 0.0001 | 0.0012 | 0.0001 | 0.0005 | 0.0004 | 0.0009 | 0.0082 | 0.0089 | 0.0024 | 0.0023 | 0.0028 |
Bud Neck | 0.0015 | 0.0001 | 0.0035 | 0.0028 | 0.0015 | 0.0023 | 0.0122 | 0.0001 | 0.0009 | 0.0001 | 0.0024 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0002 |
Bud Periphery | 0.002 | 0.0057 | 0.0007 | 0.0056 | 0.0099 | 0.0011 | 0.0015 | 0.0001 | 0.0021 | 0.0001 | 0.0007 | 0.0004 | 0.0004 | 0.0022 | 0.0057 | 0.0027 | 0.0012 | 0.0029 |
Bud Site | 0.0063 | 0.004 | 0.0094 | 0.003 | 0.0073 | 0.0005 | 0.0161 | 0.0003 | 0.0163 | 0.0001 | 0.0134 | 0.0002 | 0.0001 | 0.0023 | 0.0033 | 0.0009 | 0.0001 | 0.0002 |
Cell Periphery | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Cytoplasm | 0.0384 | 0.0434 | 0.0462 | 0.0242 | 0.1098 | 0.0817 | 0.0756 | 0.1368 | 0.0687 | 0.1364 | 0.0267 | 0.1179 | 0.0426 | 0.113 | 0.0905 | 0.0669 | 0.0821 | 0.1644 |
Cytoplasmic Foci | 0.0215 | 0.0064 | 0.0101 | 0.0093 | 0.0203 | 0.0122 | 0.0872 | 0.0215 | 0.0232 | 0.0359 | 0.0259 | 0.0598 | 0.0109 | 0.0123 | 0.0155 | 0.0341 | 0.0382 | 0.0175 |
Eisosomes | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0021 | 0.0012 | 0.0009 | 0.0011 | 0.0036 | 0.0008 | 0.0068 | 0.0006 | 0.0008 | 0.0021 | 0.0003 | 0.0007 | 0.0005 | 0.0006 | 0.0007 | 0.0008 | 0.0005 | 0.0009 |
Endosome | 0.012 | 0.0028 | 0.0031 | 0.0069 | 0.041 | 0.0127 | 0.0635 | 0.0072 | 0.0125 | 0.0404 | 0.0221 | 0.0106 | 0.02 | 0.0148 | 0.0167 | 0.0089 | 0.0336 | 0.0075 |
Golgi | 0.0081 | 0.0003 | 0.0015 | 0.0028 | 0.0086 | 0.0017 | 0.021 | 0.0011 | 0.0073 | 0.0412 | 0.0134 | 0.0035 | 0.0035 | 0.0018 | 0.0017 | 0.0033 | 0.0032 | 0.0009 |
Lipid Particles | 0.0122 | 0.0007 | 0.0028 | 0.0019 | 0.0475 | 0.0049 | 0.0305 | 0.0006 | 0.0013 | 0.0003 | 0.0358 | 0.021 | 0.001 | 0.0004 | 0.0005 | 0.0007 | 0.0003 | 0.0004 |
Mitochondria | 0.0056 | 0.0007 | 0.0051 | 0.006 | 0.0069 | 0.0024 | 0.0163 | 0.0004 | 0.0031 | 0.0002 | 0.0227 | 0.0168 | 0.0002 | 0.0003 | 0.0008 | 0.0008 | 0.0004 | 0.0005 |
None | 0.8181 | 0.9137 | 0.8887 | 0.8765 | 0.6512 | 0.8597 | 0.5415 | 0.796 | 0.8402 | 0.7374 | 0.8053 | 0.7424 | 0.9009 | 0.8407 | 0.8386 | 0.8499 | 0.8291 | 0.7913 |
Nuclear Periphery | 0.0071 | 0.0013 | 0.0011 | 0.0032 | 0.0145 | 0.002 | 0.0309 | 0.0013 | 0.0013 | 0.0009 | 0.0002 | 0.001 | 0.0017 | 0.0003 | 0.0011 | 0.0018 | 0.0004 | 0.0009 |
Nucleolus | 0.0014 | 0.0004 | 0.0011 | 0.0019 | 0.0032 | 0.0004 | 0.0013 | 0.0003 | 0.0007 | 0 | 0.0027 | 0.0076 | 0.0001 | 0.0001 | 0.0006 | 0.003 | 0.0001 | 0.0001 |
Nucleus | 0.0085 | 0.0008 | 0.0013 | 0.0041 | 0.0077 | 0.0016 | 0.0076 | 0.0133 | 0.0009 | 0.0005 | 0.0009 | 0.0022 | 0.0043 | 0.0006 | 0.003 | 0.0054 | 0.001 | 0.0017 |
Peroxisomes | 0.0155 | 0.0015 | 0.0012 | 0.0021 | 0.0173 | 0.0032 | 0.0216 | 0.0024 | 0.0019 | 0.0003 | 0.0185 | 0.0043 | 0.0015 | 0.0004 | 0.0011 | 0.0049 | 0.0047 | 0.0012 |
Punctate Nuclear | 0.0114 | 0.0016 | 0.0054 | 0.0018 | 0.0148 | 0.0024 | 0.0173 | 0.0011 | 0.0006 | 0.0004 | 0.0008 | 0.005 | 0.0023 | 0.0002 | 0.0017 | 0.0086 | 0.0009 | 0.0007 |
Vacuole | 0.0013 | 0.0075 | 0.005 | 0.003 | 0.0052 | 0.0044 | 0.0058 | 0.0122 | 0.0048 | 0.003 | 0.0045 | 0.0019 | 0.0057 | 0.0009 | 0.007 | 0.002 | 0.0006 | 0.0042 |
Vacuole Periphery | 0.001 | 0.0005 | 0.0005 | 0.0012 | 0.0021 | 0.0013 | 0.0023 | 0.0005 | 0.0023 | 0.0005 | 0.0009 | 0.0021 | 0.003 | 0.0002 | 0.0015 | 0.0008 | 0.0002 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.4504 | 26.4799 | 55.6728 | 70.3011 | 27.204 | 51.8091 | 68.0501 | 84.329 | 90.0033 | 74.6733 |
Translational Efficiency | 1.5279 | 2.426 | 1.9758 | 1.5424 | 2.823 | 1.2973 | 1.8271 | 2.5022 | 2.014 | 2.1357 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1300 | 1166 | 1258 | 1180 | 2024 | 1130 | 1573 | 1300 | 3324 | 2296 | 2831 | 2480 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1440.13 | 1313.85 | 795.25 | 760.39 | 1693.24 | 1171.87 | 790.86 | 757.56 | 1594.25 | 1243.97 | 792.81 | 758.91 |
Standard Deviation | 381.46 | 619.98 | 93.09 | 109.88 | 536.36 | 661.20 | 140.67 | 93.64 | 497.33 | 644.52 | 121.86 | 101.70 |
Intensity Change Log 2 | — | -0.132398 | -0.856719 | -0.921388 | — | -0.530974 | -1.098292 | -1.160354 | — | -0.334051 | -0.982222 | -1.045591 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005748 | 0.008324 | 0.000109 | 0.001214 | 0.003897 | 0.005281 | 0.000217 | 0.000445 | 0.004621 | 0.006827 | 0.000169 | 0.000811 |
Bud Neck | 0.002672 | 0.010147 | 0.002132 | 0.008844 | 0.001931 | 0.018903 | 0.008995 | 0.010793 | 0.002221 | 0.014457 | 0.005945 | 0.009866 |
Bud Site | 0.033066 | 0.080265 | 0.006431 | 0.050316 | 0.022878 | 0.082822 | 0.018023 | 0.032257 | 0.026863 | 0.081524 | 0.012872 | 0.040850 |
Cell Periphery | 0.000192 | 0.001033 | 0.000097 | 0.000237 | 0.000214 | 0.001004 | 0.000111 | 0.000158 | 0.000205 | 0.001018 | 0.000105 | 0.000196 |
Cytoplasm | 0.065805 | 0.192897* | 0.357978* | 0.454753* | 0.036350 | 0.321746* | 0.408149* | 0.481611* | 0.047870 | 0.256311* | 0.385855* | 0.468832* |
Cytoplasmic Foci | 0.289924* | 0.202943* | 0.012004 | 0.033469 | 0.201311* | 0.198613* | 0.005547 | 0.040985 | 0.235967* | 0.200812* | 0.008417 | 0.037409 |
Eisosomes | 0.000023 | 0.000106 | 0.000028 | 0.000060 | 0.000023 | 0.000141 | 0.000025 | 0.000060 | 0.000023 | 0.000123 | 0.000026 | 0.000060 |
Endoplasmic Reticulum | 0.000799 | 0.001775 | 0.001700 | 0.002098 | 0.000965 | 0.001084 | 0.001604 | 0.001244 | 0.000900 | 0.001435 | 0.001647 | 0.001650 |
Endosome | 0.115997 | 0.107986 | 0.001406 | 0.008755 | 0.118596 | 0.074036 | 0.001574 | 0.006199 | 0.117580 | 0.091277 | 0.001499 | 0.007415 |
Golgi | 0.274324* | 0.191410* | 0.000051 | 0.005530 | 0.405923* | 0.129891* | 0.000106 | 0.001595 | 0.354455* | 0.161133* | 0.000081 | 0.003467 |
Lipid Particles | 0.012147 | 0.014396 | 0.001015 | 0.001564 | 0.008944 | 0.007802 | 0.000712 | 0.001427 | 0.010197 | 0.011151 | 0.000846 | 0.001492 |
Mitochondria | 0.001833 | 0.005188 | 0.000361 | 0.005815 | 0.008868 | 0.006625 | 0.001656 | 0.009993 | 0.006117 | 0.005895 | 0.001080 | 0.008005 |
Mitotic Spindle | 0.091634 | 0.041879 | 0.002391 | 0.035874 | 0.073770 | 0.018101 | 0.012102 | 0.024942 | 0.080756 | 0.030176 | 0.007787 | 0.030144 |
None | 0.000912 | 0.008707 | 0.004167 | 0.005725 | 0.001932 | 0.006939 | 0.003981 | 0.003834 | 0.001533 | 0.007837 | 0.004064 | 0.004734 |
Nuclear Periphery | 0.000211 | 0.000224 | 0.001194 | 0.001639 | 0.000345 | 0.000140 | 0.001404 | 0.001060 | 0.000292 | 0.000183 | 0.001311 | 0.001336 |
Nuclear Periphery Foci | 0.019169 | 0.006435 | 0.000601 | 0.001955 | 0.013138 | 0.002783 | 0.000375 | 0.000474 | 0.015497 | 0.004638 | 0.000475 | 0.001179 |
Nucleolus | 0.000474 | 0.001416 | 0.000385 | 0.000574 | 0.000536 | 0.001560 | 0.000296 | 0.000422 | 0.000512 | 0.001487 | 0.000336 | 0.000494 |
Nucleus | 0.001361 | 0.008207 | 0.582224* | 0.241077* | 0.001846 | 0.018323 | 0.499944* | 0.258258* | 0.001656 | 0.013185 | 0.536507* | 0.250083* |
Peroxisomes | 0.006497 | 0.017485 | 0.000184 | 0.002099 | 0.002097 | 0.012129 | 0.000365 | 0.001409 | 0.003818 | 0.014849 | 0.000285 | 0.001737 |
Vacuole | 0.067967 | 0.085698 | 0.025403 | 0.133621 | 0.075307 | 0.081031 | 0.034510 | 0.120935 | 0.072436 | 0.083401 | 0.030463 | 0.126971 |
Vacuole Periphery | 0.009245 | 0.013479 | 0.000137 | 0.004782 | 0.021131 | 0.011047 | 0.000306 | 0.001897 | 0.016482 | 0.012282 | 0.000231 | 0.003270 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.66 | 5.90 | 5.19 | 6.91 | -2.72 | -1.11 | 5.43 | 5.27 | 4.39 | -1.42 | -2.25 | 8.00 | 7.31 | 7.99 | -3.01 |
Bud Neck | -6.08 | 2.67 | -5.44 | 2.35 | -7.17 | -9.59 | -4.87 | -7.53 | 5.23 | -0.44 | -11.50 | -4.06 | -9.56 | 5.58 | -2.91 |
Bud Site | -9.35 | 12.03 | 2.50 | 10.10 | -5.86 | -12.38 | 4.39 | 1.38 | 12.19 | -2.08 | -15.87 | 10.83 | 2.40 | 15.83 | -5.51 |
Cell Periphery | -7.91 | 6.54 | 1.46 | 8.17 | -4.23 | -5.37 | 6.26 | 5.24 | 6.12 | -2.02 | -9.00 | 8.55 | 4.77 | 9.84 | -4.61 |
Cytoplasm | -12.36 | -29.23 | -31.07 | -15.04 | -3.33 | -27.59 | -45.26 | -44.67 | -6.75 | -4.61 | -28.95 | -53.35 | -54.27 | -15.27 | -5.51 |
Cytoplasmic Foci | 10.22 | 51.16 | 47.70 | 30.00 | -6.34 | 0.09 | 55.10 | 41.28 | 25.18 | -14.35 | 5.94 | 72.97 | 61.84 | 38.77 | -15.32 |
Eisosomes | -2.41 | -5.88 | -16.50 | 1.02 | -10.07 | -10.24 | -4.85 | -17.47 | 6.14 | -13.08 | -5.47 | -7.52 | -23.89 | 2.90 | -16.42 |
Endoplasmic Reticulum | -3.90 | -9.83 | -12.90 | -5.00 | -3.42 | -0.32 | -7.45 | -4.95 | -3.97 | 3.65 | -3.07 | -11.41 | -12.20 | -6.09 | -0.29 |
Endosome | 0.95 | 35.14* | 33.22* | 27.42 | -4.09 | 10.86 | 44.80* | 43.03* | 21.91 | -5.11 | 7.86 | 56.93* | 54.22* | 34.79 | -6.38 |
Golgi | 7.17 | 38.39* | 37.70* | 24.91 | -2.33 | 29.30* | 62.58* | 62.40* | 19.00 | -2.73 | 26.66* | 71.12* | 70.49* | 31.06 | -3.06 |
Lipid Particles | -1.09 | 11.12 | 10.63 | 9.25 | -3.09 | 1.38 | 10.79 | 9.77 | 6.95 | -4.58 | -0.51 | 15.36 | 14.28 | 11.49 | -5.61 |
Mitochondria | -2.92 | 4.89 | -1.32 | 1.82 | -3.47 | 1.40 | 6.57 | 1.94 | 0.65 | -3.02 | 0.17 | 7.35 | 1.83 | 1.53 | -4.16 |
Mitotic Spindle | 8.11 | 17.93* | 11.84 | 4.23 | -6.53 | 13.65* | 16.12* | 12.96 | 0.63 | -2.34 | 14.07 | 24.06 | 17.30 | 3.98 | -6.06 |
None | -5.76 | -7.35 | -3.62 | 3.07 | -0.15 | -4.66 | -2.62 | -1.68 | 3.70 | 0.78 | -7.07 | -5.07 | -3.41 | 4.63 | 0.32 |
Nuclear Periphery | 0.42 | -24.23 | -13.30 | -13.29 | -6.85 | 7.86 | -24.50 | -16.22 | -19.85 | 2.31 | 5.69 | -32.64 | -18.98 | -20.45 | -4.53 |
Nuclear Periphery Foci | 6.18 | 9.62 | 9.10 | 4.87 | -2.46 | 7.36 | 9.73 | 9.70 | 4.78 | -0.74 | 9.06 | 13.66 | 13.16 | 6.56 | -2.61 |
Nucleolus | -2.60 | 0.99 | -0.13 | 2.62 | -1.54 | -2.62 | 1.48 | 0.84 | 3.32 | -2.50 | -3.63 | 1.72 | 0.57 | 4.19 | -2.56 |
Nucleus | -6.76 | -72.33 | -37.46 | -36.28 | 28.82 | -10.89 | -73.29* | -41.24 | -38.21 | 24.28 | -12.51 | -102.33* | -55.66 | -52.77 | 37.24 |
Peroxisomes | -5.88 | 11.78 | 9.06 | 9.10 | -3.65 | -9.16 | 8.87 | 4.23 | 10.22 | -3.71 | -10.20 | 14.58 | 9.64 | 13.01 | -5.15 |
Vacuole | -2.04 | 7.85 | -19.95 | -17.43 | -25.13 | -0.60 | 4.70 | -16.85 | -15.06 | -19.39 | -1.85 | 8.74 | -26.00 | -22.88 | -31.21 |
Vacuole Periphery | -3.32 | 11.91 | 7.08 | 8.85 | -2.88 | 7.06 | 18.57 | 17.05 | 10.44 | -2.26 | 4.01 | 21.55 | 17.88 | 13.41 | -3.62 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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