Standard name
Human Ortholog
Description Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation by targeting Pho87p to the vacuole; upstream region harbors putative hypoxia response element (HRE) cluster; overproduction suppresses a plc1 null mutation; promoter shows an increase in Snf2p occupancy after heat shock; GFP-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0.05 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.96 0.95 0.93 0.95 0.9 – 0.81 0.82 0.8 0.68 0.74 0.97 0.95 0.96 0.68 0.67 0.69 0.54 0.7 0.64 0.55 0.56 0.62
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0.1 0 0.09 0.09 0.09 0.08
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0.07 0.05 0 0 0 0.06 0
Mitochondria 0 0 0 0 0 0.18 – 0.13 0.24 0.22 0.33 0.3 0 0 0 0 0 0 0 0.05 0.07 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0 – 0.06 0.05 0 0.05 0 0 0 0 0.28 0.4 0.24 0.14 0.1 0.05 0.17 0.11 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 2 1 2 1 – 3 1 5 0 4 0 1 1 2 0 1 4 1 3 1 6 4
Bud 2 0 3 4 7 5 – 18 18 24 26 40 0 2 1 0 1 2 7 0 2 2 14 11
Bud Neck 0 0 0 2 8 2 – 10 5 6 0 5 0 0 2 0 0 0 1 0 0 1 0 0
Bud Site 0 0 0 1 0 0 – 2 2 4 3 1 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 – 1 0 3 0 0 1 1 0 1 1 2 0 0 0 0 0 0
Cytoplasm 191 65 91 286 466 279 – 476 343 423 248 465 165 348 447 136 39 93 128 63 57 38 136 158
Endoplasmic Reticulum 3 0 0 2 1 1 – 0 0 1 1 2 2 4 5 7 4 9 1 0 0 0 2 2
Endosome 2 1 1 5 1 1 – 13 2 13 6 5 1 2 2 9 2 6 24 3 8 6 21 19
Golgi 8 2 0 1 1 0 – 0 0 0 0 1 1 0 7 12 2 9 12 0 0 3 15 7
Mitochondria 5 0 4 8 11 55 – 74 100 114 121 189 2 4 2 5 2 3 9 4 6 2 9 8
Nucleus 0 0 0 1 3 2 – 11 2 5 3 4 0 0 0 1 0 1 1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 – 4 2 4 6 6 0 0 0 0 0 0 2 0 0 0 0 1
Nucleolus 3 1 0 2 0 1 – 6 2 3 6 6 0 2 1 1 0 4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 – 0 0 0 0 1 1 4 0 0 0 0 4 0 0 0 0 0
SpindlePole 0 0 0 3 1 1 – 12 2 7 4 12 1 5 3 0 0 0 3 1 1 0 4 5
Vac/Vac Membrane 13 2 1 10 15 6 – 33 19 17 18 22 1 3 6 55 23 32 32 9 4 11 26 31
Unique Cell Count 216 68 96 306 491 310 589 416 528 365 626 170 365 468 199 58 134 239 91 89 70 244 255
Labelled Cell Count 229 72 102 326 517 355 663 498 629 442 763 175 377 477 229 74 162 239 91 89 70 244 255


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.5 6.9 5.5 5.5 5.3 4.1 – 4.8 4.0 4.0 4.4 4.2 9.3 11.6 8.9 18.4 21.6 20.2 11.0 9.2 7.8
Std Deviation (1e-4) 11.3 7.9 5.5 5.8 4.3 3.1 – 3.5 2.4 1.9 2.5 2.3 11.5 13.1 9.7 14.9 17.0 16.5 10.2 9.5 7.6
Intensity Change (Log2) – – – -0.01 -0.08 -0.43 – -0.21 -0.46 -0.47 -0.34 -0.42 0.74 1.07 0.69 1.73 1.96 1.87 0.98 0.74 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 0 -1.4 – -3.4 -3.0 -3.5 -5.3 -4.4 0.9 0.2 0.3 -5.1 -4.6 -4.7
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 5.5 6.4 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2616 -0.6013 -0.4018 -0.6185 -0.1678 -0.0516 0.1141 -0.2121 -0.1865 0.855 -1.5438 0.4466 0.5646 2.2462 1.589 1.7931 1.6495 1.7445
Actin 0.0245 0.0042 0.0108 0.0322 0.0135 0.0036 0.0385 0.003 0.0094 0.0002 0.0017 0.0018 0.0002 0.0001 0.0006 0.0014 0.0007 0.0006
Bud 0.001 0.0028 0.0008 0.0097 0.0134 0.0009 0.0013 0.0001 0.0012 0.0001 0.0005 0.0004 0.0009 0.0082 0.0089 0.0024 0.0023 0.0028
Bud Neck 0.0015 0.0001 0.0035 0.0028 0.0015 0.0023 0.0122 0.0001 0.0009 0.0001 0.0024 0.0004 0.0001 0.0001 0.0002 0.0004 0.0002 0.0002
Bud Periphery 0.002 0.0057 0.0007 0.0056 0.0099 0.0011 0.0015 0.0001 0.0021 0.0001 0.0007 0.0004 0.0004 0.0022 0.0057 0.0027 0.0012 0.0029
Bud Site 0.0063 0.004 0.0094 0.003 0.0073 0.0005 0.0161 0.0003 0.0163 0.0001 0.0134 0.0002 0.0001 0.0023 0.0033 0.0009 0.0001 0.0002
Cell Periphery 0.0002 0.0001 0.0004 0.0003 0.0003 0.0001 0.0006 0.0001 0.0003 0 0.0005 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002
Cytoplasm 0.0384 0.0434 0.0462 0.0242 0.1098 0.0817 0.0756 0.1368 0.0687 0.1364 0.0267 0.1179 0.0426 0.113 0.0905 0.0669 0.0821 0.1644
Cytoplasmic Foci 0.0215 0.0064 0.0101 0.0093 0.0203 0.0122 0.0872 0.0215 0.0232 0.0359 0.0259 0.0598 0.0109 0.0123 0.0155 0.0341 0.0382 0.0175
Eisosomes 0.0004 0.0002 0.0005 0.0002 0.0002 0.0001 0.0005 0.001 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0021 0.0012 0.0009 0.0011 0.0036 0.0008 0.0068 0.0006 0.0008 0.0021 0.0003 0.0007 0.0005 0.0006 0.0007 0.0008 0.0005 0.0009
Endosome 0.012 0.0028 0.0031 0.0069 0.041 0.0127 0.0635 0.0072 0.0125 0.0404 0.0221 0.0106 0.02 0.0148 0.0167 0.0089 0.0336 0.0075
Golgi 0.0081 0.0003 0.0015 0.0028 0.0086 0.0017 0.021 0.0011 0.0073 0.0412 0.0134 0.0035 0.0035 0.0018 0.0017 0.0033 0.0032 0.0009
Lipid Particles 0.0122 0.0007 0.0028 0.0019 0.0475 0.0049 0.0305 0.0006 0.0013 0.0003 0.0358 0.021 0.001 0.0004 0.0005 0.0007 0.0003 0.0004
Mitochondria 0.0056 0.0007 0.0051 0.006 0.0069 0.0024 0.0163 0.0004 0.0031 0.0002 0.0227 0.0168 0.0002 0.0003 0.0008 0.0008 0.0004 0.0005
None 0.8181 0.9137 0.8887 0.8765 0.6512 0.8597 0.5415 0.796 0.8402 0.7374 0.8053 0.7424 0.9009 0.8407 0.8386 0.8499 0.8291 0.7913
Nuclear Periphery 0.0071 0.0013 0.0011 0.0032 0.0145 0.002 0.0309 0.0013 0.0013 0.0009 0.0002 0.001 0.0017 0.0003 0.0011 0.0018 0.0004 0.0009
Nucleolus 0.0014 0.0004 0.0011 0.0019 0.0032 0.0004 0.0013 0.0003 0.0007 0 0.0027 0.0076 0.0001 0.0001 0.0006 0.003 0.0001 0.0001
Nucleus 0.0085 0.0008 0.0013 0.0041 0.0077 0.0016 0.0076 0.0133 0.0009 0.0005 0.0009 0.0022 0.0043 0.0006 0.003 0.0054 0.001 0.0017
Peroxisomes 0.0155 0.0015 0.0012 0.0021 0.0173 0.0032 0.0216 0.0024 0.0019 0.0003 0.0185 0.0043 0.0015 0.0004 0.0011 0.0049 0.0047 0.0012
Punctate Nuclear 0.0114 0.0016 0.0054 0.0018 0.0148 0.0024 0.0173 0.0011 0.0006 0.0004 0.0008 0.005 0.0023 0.0002 0.0017 0.0086 0.0009 0.0007
Vacuole 0.0013 0.0075 0.005 0.003 0.0052 0.0044 0.0058 0.0122 0.0048 0.003 0.0045 0.0019 0.0057 0.0009 0.007 0.002 0.0006 0.0042
Vacuole Periphery 0.001 0.0005 0.0005 0.0012 0.0021 0.0013 0.0023 0.0005 0.0023 0.0005 0.0009 0.0021 0.003 0.0002 0.0015 0.0008 0.0002 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.4504 26.4799 55.6728 70.3011 27.204 51.8091 68.0501 84.329 90.0033 74.6733
Translational Efficiency 1.5279 2.426 1.9758 1.5424 2.823 1.2973 1.8271 2.5022 2.014 2.1357

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1300 1166 1258 1180 2024 1130 1573 1300 3324 2296 2831 2480

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1440.13 1313.85 795.25 760.39 1693.24 1171.87 790.86 757.56 1594.25 1243.97 792.81 758.91
Standard Deviation 381.46 619.98 93.09 109.88 536.36 661.20 140.67 93.64 497.33 644.52 121.86 101.70
Intensity Change Log 2 — -0.132398 -0.856719 -0.921388 — -0.530974 -1.098292 -1.160354 — -0.334051 -0.982222 -1.045591

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005748 0.008324 0.000109 0.001214 0.003897 0.005281 0.000217 0.000445 0.004621 0.006827 0.000169 0.000811
Bud Neck 0.002672 0.010147 0.002132 0.008844 0.001931 0.018903 0.008995 0.010793 0.002221 0.014457 0.005945 0.009866
Bud Site 0.033066 0.080265 0.006431 0.050316 0.022878 0.082822 0.018023 0.032257 0.026863 0.081524 0.012872 0.040850
Cell Periphery 0.000192 0.001033 0.000097 0.000237 0.000214 0.001004 0.000111 0.000158 0.000205 0.001018 0.000105 0.000196
Cytoplasm 0.065805 0.192897* 0.357978* 0.454753* 0.036350 0.321746* 0.408149* 0.481611* 0.047870 0.256311* 0.385855* 0.468832*
Cytoplasmic Foci 0.289924* 0.202943* 0.012004 0.033469 0.201311* 0.198613* 0.005547 0.040985 0.235967* 0.200812* 0.008417 0.037409
Eisosomes 0.000023 0.000106 0.000028 0.000060 0.000023 0.000141 0.000025 0.000060 0.000023 0.000123 0.000026 0.000060
Endoplasmic Reticulum 0.000799 0.001775 0.001700 0.002098 0.000965 0.001084 0.001604 0.001244 0.000900 0.001435 0.001647 0.001650
Endosome 0.115997 0.107986 0.001406 0.008755 0.118596 0.074036 0.001574 0.006199 0.117580 0.091277 0.001499 0.007415
Golgi 0.274324* 0.191410* 0.000051 0.005530 0.405923* 0.129891* 0.000106 0.001595 0.354455* 0.161133* 0.000081 0.003467
Lipid Particles 0.012147 0.014396 0.001015 0.001564 0.008944 0.007802 0.000712 0.001427 0.010197 0.011151 0.000846 0.001492
Mitochondria 0.001833 0.005188 0.000361 0.005815 0.008868 0.006625 0.001656 0.009993 0.006117 0.005895 0.001080 0.008005
Mitotic Spindle 0.091634 0.041879 0.002391 0.035874 0.073770 0.018101 0.012102 0.024942 0.080756 0.030176 0.007787 0.030144
None 0.000912 0.008707 0.004167 0.005725 0.001932 0.006939 0.003981 0.003834 0.001533 0.007837 0.004064 0.004734
Nuclear Periphery 0.000211 0.000224 0.001194 0.001639 0.000345 0.000140 0.001404 0.001060 0.000292 0.000183 0.001311 0.001336
Nuclear Periphery Foci 0.019169 0.006435 0.000601 0.001955 0.013138 0.002783 0.000375 0.000474 0.015497 0.004638 0.000475 0.001179
Nucleolus 0.000474 0.001416 0.000385 0.000574 0.000536 0.001560 0.000296 0.000422 0.000512 0.001487 0.000336 0.000494
Nucleus 0.001361 0.008207 0.582224* 0.241077* 0.001846 0.018323 0.499944* 0.258258* 0.001656 0.013185 0.536507* 0.250083*
Peroxisomes 0.006497 0.017485 0.000184 0.002099 0.002097 0.012129 0.000365 0.001409 0.003818 0.014849 0.000285 0.001737
Vacuole 0.067967 0.085698 0.025403 0.133621 0.075307 0.081031 0.034510 0.120935 0.072436 0.083401 0.030463 0.126971
Vacuole Periphery 0.009245 0.013479 0.000137 0.004782 0.021131 0.011047 0.000306 0.001897 0.016482 0.012282 0.000231 0.003270

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.66 5.90 5.19 6.91 -2.72 -1.11 5.43 5.27 4.39 -1.42 -2.25 8.00 7.31 7.99 -3.01
Bud Neck -6.08 2.67 -5.44 2.35 -7.17 -9.59 -4.87 -7.53 5.23 -0.44 -11.50 -4.06 -9.56 5.58 -2.91
Bud Site -9.35 12.03 2.50 10.10 -5.86 -12.38 4.39 1.38 12.19 -2.08 -15.87 10.83 2.40 15.83 -5.51
Cell Periphery -7.91 6.54 1.46 8.17 -4.23 -5.37 6.26 5.24 6.12 -2.02 -9.00 8.55 4.77 9.84 -4.61
Cytoplasm -12.36 -29.23 -31.07 -15.04 -3.33 -27.59 -45.26 -44.67 -6.75 -4.61 -28.95 -53.35 -54.27 -15.27 -5.51
Cytoplasmic Foci 10.22 51.16 47.70 30.00 -6.34 0.09 55.10 41.28 25.18 -14.35 5.94 72.97 61.84 38.77 -15.32
Eisosomes -2.41 -5.88 -16.50 1.02 -10.07 -10.24 -4.85 -17.47 6.14 -13.08 -5.47 -7.52 -23.89 2.90 -16.42
Endoplasmic Reticulum -3.90 -9.83 -12.90 -5.00 -3.42 -0.32 -7.45 -4.95 -3.97 3.65 -3.07 -11.41 -12.20 -6.09 -0.29
Endosome 0.95 35.14* 33.22* 27.42 -4.09 10.86 44.80* 43.03* 21.91 -5.11 7.86 56.93* 54.22* 34.79 -6.38
Golgi 7.17 38.39* 37.70* 24.91 -2.33 29.30* 62.58* 62.40* 19.00 -2.73 26.66* 71.12* 70.49* 31.06 -3.06
Lipid Particles -1.09 11.12 10.63 9.25 -3.09 1.38 10.79 9.77 6.95 -4.58 -0.51 15.36 14.28 11.49 -5.61
Mitochondria -2.92 4.89 -1.32 1.82 -3.47 1.40 6.57 1.94 0.65 -3.02 0.17 7.35 1.83 1.53 -4.16
Mitotic Spindle 8.11 17.93* 11.84 4.23 -6.53 13.65* 16.12* 12.96 0.63 -2.34 14.07 24.06 17.30 3.98 -6.06
None -5.76 -7.35 -3.62 3.07 -0.15 -4.66 -2.62 -1.68 3.70 0.78 -7.07 -5.07 -3.41 4.63 0.32
Nuclear Periphery 0.42 -24.23 -13.30 -13.29 -6.85 7.86 -24.50 -16.22 -19.85 2.31 5.69 -32.64 -18.98 -20.45 -4.53
Nuclear Periphery Foci 6.18 9.62 9.10 4.87 -2.46 7.36 9.73 9.70 4.78 -0.74 9.06 13.66 13.16 6.56 -2.61
Nucleolus -2.60 0.99 -0.13 2.62 -1.54 -2.62 1.48 0.84 3.32 -2.50 -3.63 1.72 0.57 4.19 -2.56
Nucleus -6.76 -72.33 -37.46 -36.28 28.82 -10.89 -73.29* -41.24 -38.21 24.28 -12.51 -102.33* -55.66 -52.77 37.24
Peroxisomes -5.88 11.78 9.06 9.10 -3.65 -9.16 8.87 4.23 10.22 -3.71 -10.20 14.58 9.64 13.01 -5.15
Vacuole -2.04 7.85 -19.95 -17.43 -25.13 -0.60 4.70 -16.85 -15.06 -19.39 -1.85 8.74 -26.00 -22.88 -31.21
Vacuole Periphery -3.32 11.91 7.08 8.85 -2.88 7.06 18.57 17.05 10.44 -2.26 4.01 21.55 17.88 13.41 -3.62
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation by targeting Pho87p to the vacuole; upstream region harbors putative hypoxia response element (HRE) cluster; overproduction suppresses a plc1 null mutation; promoter shows an increase in Snf2p occupancy after heat shock; GFP-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (16%), vacuole (7%), mixed (50%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (0.00022)
vacuole - S/G2 (0.00076)
Subcompartmental Group N/A

Spl2

Spl2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spl2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available