Standard name
Human Ortholog
Description Aromatic aminotransferase II; catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0.06 0.11
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.97 – 0.94 0.88 0.86 0.83 0.73 0.73 0.64 0.6 0.98 0.99 0.99 0.88 0.9 0.94 0.85 0.85 0.89 0.87 0.86 0.77
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0.09 0.2 0.32 0.25 0.39 0.44 0.57 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.14 0.15 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 2 1
Bud 0 2 2 – 3 0 0 0 2 0 1 5 1 0 0 1 0 1 28 20 5 9 17 37
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 1
Bud Site 0 0 0 – 0 0 0 1 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 – 0 1 1 0 1 1 0 1 0 2 0 4 2 1 0 0 0 0 0 0
Cytoplasm 334 360 131 – 99 73 106 121 94 124 52 94 348 489 397 611 431 229 287 325 122 214 257 265
Endoplasmic Reticulum 0 1 0 – 0 0 0 0 2 0 0 0 0 2 0 23 12 5 1 0 0 1 0 5
Endosome 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 3 1 0 3 1
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 4 5 1 0 1 0 0 0 1
Mitochondria 3 3 2 – 9 17 39 36 50 74 46 87 0 2 3 4 0 3 2 4 1 4 2 2
Nucleus 1 0 0 – 0 0 0 1 0 3 0 0 1 1 0 2 0 1 1 4 0 3 1 2
Nuclear Periphery 0 0 0 – 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3 3 1 3 4 4
Vac/Vac Membrane 3 11 2 – 2 1 5 2 2 3 0 1 11 9 5 96 70 17 1 11 2 1 2 10
Unique Cell Count 336 374 135 105 83 123 145 129 170 81 157 354 493 399 692 480 243 337 383 138 245 299 343
Labelled Cell Count 342 378 137 115 92 154 161 151 207 99 188 361 506 405 746 520 258 337 383 138 245 299 343


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.6 9.8 7.2 – 5.9 4.7 3.9 4.0 3.6 3.3 3.0 3.2 8.0 7.0 6.6 27.9 34.2 34.5 6.4 6.8 7.4
Std Deviation (1e-4) 4.6 6.3 6.0 – 2.5 2.1 2.1 1.4 1.4 1.9 0.9 2.0 6.3 2.8 2.4 14.0 17.5 13.9 2.4 2.6 2.5
Intensity Change (Log2) – – – – -0.27 -0.63 -0.89 -0.83 -0.99 -1.12 -1.26 -1.18 0.16 -0.03 -0.13 1.96 2.25 2.26 -0.17 -0.09 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – -1.1 -2.7 -3.2 -3.8 -5.5 -5.6 -6.5 -7.5 0.9 2.0 2.3 -3.1 -2.7 -1.2
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole – 0 0 0 0 0 0 0 0 0 0 0 4.1 4.2 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7347 20.1046 19.8791 4.9689 10.8237 10.989 5.9827 8.4656 7.5686 2.4779 11.9007 7.4477 13.2493 20.4142 11.3757 7.8824 3.7724 11.5268
Actin 0.0649 0.0011 0.0068 0.0053 0.0055 0.0058 0.0511 0.001 0.0115 0.0232 0.0049 0.0059 0.0705 0.0041 0.0061 0.0252 0.013 0.0166
Bud 0.0019 0.0003 0.0052 0.051 0.0009 0.0015 0.0016 0.0081 0.0095 0.0392 0.0002 0.01 0.0017 0.0169 0.0207 0.009 0.0301 0.008
Bud Neck 0.0007 0.0001 0.0003 0.0005 0.0004 0.0015 0.0052 0.0004 0.0003 0.0042 0.0005 0.0009 0.0112 0.0015 0.0026 0.0014 0.0006 0.0016
Bud Periphery 0.0029 0.0004 0.0079 0.0141 0.0063 0.0007 0.0023 0.0037 0.0052 0.0261 0.0001 0.0061 0.0043 0.0099 0.0066 0.0035 0.0102 0.0045
Bud Site 0.0018 0.0005 0.0055 0.0021 0.0002 0.0003 0.0101 0.0076 0.0081 0.0409 0.0009 0.0008 0.0147 0.0229 0.01 0.0022 0.0022 0.001
Cell Periphery 0.0002 0.0003 0.0011 0.0004 0.0006 0.0002 0.0004 0.0002 0.0003 0.001 0.0001 0.0004 0.0017 0.0009 0.0021 0.0008 0.0003 0.0002
Cytoplasm 0.2828 0.6569 0.6283 0.6811 0.6036 0.7026 0.4324 0.5104 0.5739 0.46 0.3013 0.582 0.4482 0.6678 0.6067 0.484 0.5397 0.5845
Cytoplasmic Foci 0.0102 0.0038 0.0119 0.0131 0.0031 0.0185 0.0447 0.0267 0.014 0.0173 0.0229 0.0171 0.0441 0.0073 0.0276 0.0196 0.0084 0.0153
Eisosomes 0.0006 0.0001 0.0003 0.0001 0.0001 0 0.0006 0.0001 0.0001 0.0002 0.0002 0.0001 0.0018 0.0005 0.0002 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.013 0.0015 0.0112 0.0024 0.0378 0.0079 0.0041 0.003 0.0033 0.0021 0.0025 0.0031 0.01 0.0031 0.0065 0.0026 0.0031 0.003
Endosome 0.0933 0.0006 0.0142 0.001 0.0069 0.0039 0.0335 0.0096 0.0021 0.0054 0.0324 0.0037 0.0316 0.0009 0.0073 0.0057 0.008 0.0079
Golgi 0.0208 0.0002 0.0021 0.0002 0.0011 0.0005 0.0086 0.0023 0.0006 0.0025 0.0065 0.0008 0.0118 0.0003 0.0013 0.0025 0.001 0.002
Lipid Particles 0.0108 0.0001 0.0039 0.0002 0.0002 0.0008 0.0172 0.0005 0.0006 0.0012 0.0077 0.0005 0.0306 0.0004 0.0013 0.0036 0.0016 0.0053
Mitochondria 0.0184 0.0001 0.0174 0.0086 0.0154 0.0003 0.0028 0.0011 0.0008 0.0328 0.0007 0.001 0.0433 0.0014 0.0027 0.0055 0.0035 0.0021
None 0.442 0.331 0.2615 0.1917 0.2902 0.2352 0.3469 0.4165 0.3501 0.3328 0.5623 0.3327 0.2003 0.2393 0.2689 0.3816 0.3373 0.318
Nuclear Periphery 0.0158 0.0006 0.0051 0.0044 0.0106 0.0041 0.0041 0.0007 0.0016 0.001 0.0051 0.003 0.0158 0.0023 0.0039 0.0049 0.0087 0.0036
Nucleolus 0.0003 0 0.0006 0.0011 0.0001 0.0001 0.002 0.0004 0.0005 0.0002 0.0017 0.0007 0.0111 0.0013 0.0018 0.002 0.0012 0.001
Nucleus 0.0018 0.0012 0.0048 0.0142 0.0103 0.0105 0.0036 0.0018 0.0033 0.0033 0.006 0.0141 0.009 0.0107 0.0079 0.0198 0.0113 0.0084
Peroxisomes 0.0061 0.0001 0.0014 0.0006 0 0.0002 0.017 0.0015 0.0072 0.0023 0.0029 0.0102 0.0222 0.0003 0.0023 0.0023 0.001 0.0044
Punctate Nuclear 0.0027 0.0003 0.0077 0.0048 0.0003 0.0024 0.008 0.0031 0.0058 0.0016 0.0402 0.0043 0.0113 0.0066 0.008 0.0178 0.0164 0.0103
Vacuole 0.0037 0.0005 0.0019 0.002 0.0033 0.0022 0.003 0.0012 0.001 0.002 0.0007 0.002 0.0034 0.0014 0.0043 0.0042 0.0018 0.0016
Vacuole Periphery 0.0052 0.0001 0.0009 0.0008 0.0031 0.0008 0.0008 0.0001 0.0002 0.0006 0.0003 0.0005 0.0015 0.0002 0.0012 0.0015 0.0007 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5224 24.9473 28.4799 28.9936 22.2663 12.1579 36.8661 37.978 35.6607 26.44
Translational Efficiency 0.8781 0.8837 0.8018 0.8482 1.1276 0.8442 0.949 0.8055 0.7991 0.6677

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1806 1387 1678 577 239 1473 122 137 2045 2860 1800 714

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 760.28 2018.48 4428.21 6387.70 1009.18 2160.99 6756.19 7457.62 789.37 2091.88 4586.00 6592.99
Standard Deviation 396.75 1302.17 3516.54 2213.33 386.27 1251.60 3390.21 2551.19 403.54 1278.36 3556.59 2320.61
Intensity Change Log 2 — 1.408667 2.542121 3.070694 — 1.098509 2.743026 2.885532 — 1.240011 2.660107 2.968017

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000100 0.000193 0.000116 0.000095 0.000348 0.000172 0.000439 0.003279 0.000129 0.000182 0.000138 0.000706
Bud Neck 0.006078 0.000907 0.001581 0.000873 0.001912 0.001112 0.007410 0.013968 0.005591 0.001012 0.001976 0.003386
Bud Site 0.006902 0.003719 0.003405 0.003187 0.007651 0.004478 0.002516 0.037181 0.006990 0.004110 0.003345 0.009710
Cell Periphery 0.000253 0.000261 0.000619 0.001177 0.000312 0.000148 0.003983 0.001732 0.000260 0.000203 0.000847 0.001284
Cytoplasm 0.672787* 0.737241* 0.631532* 0.716730* 0.636501* 0.758383* 0.389980* 0.396634* 0.668547* 0.748130* 0.615161* 0.655311*
Cytoplasmic Foci 0.071632 0.011795 0.018128 0.012433 0.148833 0.008401 0.038592 0.105799 0.080655 0.010047 0.019515 0.030347
Eisosomes 0.000067 0.000002 0.000002 0.000002 0.000092 0.000002 0.000010 0.000046 0.000070 0.000002 0.000002 0.000010
Endoplasmic Reticulum 0.000372 0.000258 0.000038 0.000025 0.001613 0.000215 0.000394 0.002219 0.000517 0.000236 0.000062 0.000446
Endosome 0.002588 0.000688 0.000093 0.002345 0.010147 0.000484 0.000718 0.063985 0.003471 0.000582 0.000136 0.014172
Golgi 0.001024 0.001614 0.000343 0.001762 0.000628 0.000674 0.002242 0.074333 0.000978 0.001130 0.000472 0.015687
Lipid Particles 0.002341 0.000229 0.000265 0.000825 0.004005 0.000235 0.005254 0.004880 0.002536 0.000232 0.000603 0.001603
Mitochondria 0.001424 0.000343 0.000391 0.000292 0.000189 0.000223 0.000592 0.039579 0.001280 0.000281 0.000405 0.007831
Mitotic Spindle 0.003080 0.007556 0.003282 0.000451 0.000189 0.014207 0.001703 0.023163 0.002742 0.010981 0.003175 0.004809
None 0.024698 0.019882 0.017849 0.014532 0.004084 0.021070 0.007664 0.005961 0.022288 0.020494 0.017159 0.012887
Nuclear Periphery 0.000248 0.000119 0.000267 0.000153 0.000237 0.000179 0.001636 0.005589 0.000247 0.000150 0.000360 0.001196
Nuclear Periphery Foci 0.000751 0.001805 0.000450 0.001198 0.005713 0.001234 0.004079 0.006707 0.001331 0.001511 0.000696 0.002255
Nucleolus 0.001623 0.000160 0.000186 0.000064 0.000338 0.000283 0.000175 0.002116 0.001473 0.000223 0.000185 0.000458
Nucleus 0.185416* 0.206319* 0.316633* 0.224360* 0.088886 0.182229* 0.499400* 0.098897 0.174135* 0.193912* 0.329020* 0.200286*
Peroxisomes 0.001007 0.000051 0.000016 0.000055 0.000799 0.000103 0.000017 0.001713 0.000983 0.000078 0.000016 0.000373
Vacuole 0.017307 0.005843 0.004647 0.018111 0.087115 0.005971 0.032504 0.080660 0.025466 0.005909 0.006535 0.030113
Vacuole Periphery 0.000300 0.001013 0.000155 0.001330 0.000408 0.000198 0.000692 0.031559 0.000313 0.000593 0.000192 0.007130

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.97 -3.88 -2.61 2.45 1.22 3.16 -0.40 -0.63 -1.26 -0.52 -3.27 -2.93 -1.62 -0.32 -0.75
Bud Neck 6.88 4.63 5.97 -3.58 3.85 1.60 -3.93 -3.10 -3.33 -0.97 6.84 3.97 2.84 -3.98 -0.60
Bud Site 2.65 2.84 3.29 0.72 0.96 2.34 3.90 -1.52 -2.25 -2.60 2.79 3.35 1.08 -1.15 -1.48
Cell Periphery -1.37 -10.73 -8.31 -7.90 -4.05 3.47 -8.08 -4.28 -4.78 4.12 1.26 -12.66 -9.40 -9.58 -3.12
Cytoplasm -6.28 10.73 0.81 5.66 -7.33 -6.38 12.83 4.05 9.20 -6.08 -9.05 12.58 3.21 9.71 -6.12
Cytoplasmic Foci 20.87 16.79 16.54 -3.15 0.60 14.42 7.60 7.34 -5.88 0.46 26.46 18.82 15.96 -6.94 -0.98
Eisosomes 9.66 9.61 9.41 -4.02 -3.34 11.72 9.79 9.53 -3.43 0.43 11.29 11.11 10.67 -4.69 -3.17
Endoplasmic Reticulum 1.03 6.15 7.04 2.19 4.79 5.62 5.59 4.50 -0.62 -0.88 3.59 8.32 7.19 2.39 -0.29
Endosome 6.45 9.81 2.80 -0.35 -1.20 10.67 10.75 0.34 -2.55 -2.60 10.90 13.26 1.30 -2.30 -2.81
Golgi -0.56 1.68 0.42 0.85 -0.85 0.07 -1.43 -2.03 -2.04 -1.90 -0.05 1.57 -1.60 -1.58 -2.17
Lipid Particles 4.91 3.95 0.85 -6.39 -4.79 3.98 -0.85 -0.68 -3.02 -0.02 5.94 3.89 -0.11 -5.98 -3.97
Mitochondria 3.70 3.45 4.51 1.41 4.80 -1.23 -2.96 -1.38 -1.36 -1.32 4.00 3.32 0.09 -1.21 -0.97
Mitotic Spindle -2.38 -1.95 2.82 4.27 7.11 -6.71 -2.30 -2.21 2.60 -1.42 -5.18 -2.26 1.40 6.69 4.64
None 3.08 4.98 7.02 4.84 3.66 -8.70 -1.69 -0.45 10.48 1.89 2.11 4.50 7.31 7.46 4.54
Nuclear Periphery 5.41 -8.58 -2.42 -7.20 6.29 -0.08 -9.71 -2.04 -2.04 0.64 2.81 -11.34 -2.37 -2.74 0.52
Nuclear Periphery Foci -5.91 -0.69 -4.75 1.88 -4.63 5.08 2.64 2.76 -2.50 0.06 -2.54 2.42 -2.61 -0.50 -4.86
Nucleolus 3.67 3.57 3.93 4.23 11.14 0.54 4.42 -0.90 -1.02 -1.49 3.54 3.62 3.68 0.88 0.67
Nucleus -4.57 -23.51 -6.16 -2.40 11.87 -11.72 -20.83 -5.22 2.67 14.75 -6.84 -27.90 -6.64 -1.79 15.72
Peroxisomes 4.92 5.10 5.07 1.46 -0.23 4.23 4.91 2.82 -1.67 -2.73 5.21 5.59 5.25 0.29 -2.35
Vacuole 5.48 1.93 -7.84 -9.34 -8.34 10.47 1.16 -4.31 -9.74 -4.92 10.39 4.00 -9.40 -12.60 -10.80
Vacuole Periphery -1.28 2.89 -0.72 0.63 -1.12 4.10 0.28 -1.69 -1.79 -1.71 -0.91 3.13 -1.84 -1.37 -2.07
Standard name
Human Ortholog
Description Aromatic aminotransferase II; catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Aro9

Aro9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aro9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available