Standard name | |
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Human Ortholog | |
Description | Aromatic aminotransferase II; catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 2 | 1 |
Bud | 0 | 2 | 2 | – | 3 | 0 | 0 | 0 | 2 | 0 | 1 | 5 | 1 | 0 | 0 | 1 | 0 | 1 | 28 | 20 | 5 | 9 | 17 | 37 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | – | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 0 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 334 | 360 | 131 | – | 99 | 73 | 106 | 121 | 94 | 124 | 52 | 94 | 348 | 489 | 397 | 611 | 431 | 229 | 287 | 325 | 122 | 214 | 257 | 265 |
Endoplasmic Reticulum | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 23 | 12 | 5 | 1 | 0 | 0 | 1 | 0 | 5 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 3 | 1 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 5 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 3 | 3 | 2 | – | 9 | 17 | 39 | 36 | 50 | 74 | 46 | 87 | 0 | 2 | 3 | 4 | 0 | 3 | 2 | 4 | 1 | 4 | 2 | 2 |
Nucleus | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 1 | 4 | 0 | 3 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 1 | 3 | 4 | 4 |
Vac/Vac Membrane | 3 | 11 | 2 | – | 2 | 1 | 5 | 2 | 2 | 3 | 0 | 1 | 11 | 9 | 5 | 96 | 70 | 17 | 1 | 11 | 2 | 1 | 2 | 10 |
Unique Cell Count | 336 | 374 | 135 | 105 | 83 | 123 | 145 | 129 | 170 | 81 | 157 | 354 | 493 | 399 | 692 | 480 | 243 | 337 | 383 | 138 | 245 | 299 | 343 | |
Labelled Cell Count | 342 | 378 | 137 | 115 | 92 | 154 | 161 | 151 | 207 | 99 | 188 | 361 | 506 | 405 | 746 | 520 | 258 | 337 | 383 | 138 | 245 | 299 | 343 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.6 | 9.8 | 7.2 | – | 5.9 | 4.7 | 3.9 | 4.0 | 3.6 | 3.3 | 3.0 | 3.2 | 8.0 | 7.0 | 6.6 | 27.9 | 34.2 | 34.5 | 6.4 | 6.8 | 7.4 |
Std Deviation (1e-4) | 4.6 | 6.3 | 6.0 | – | 2.5 | 2.1 | 2.1 | 1.4 | 1.4 | 1.9 | 0.9 | 2.0 | 6.3 | 2.8 | 2.4 | 14.0 | 17.5 | 13.9 | 2.4 | 2.6 | 2.5 |
Intensity Change (Log2) | – | – | – | – | -0.27 | -0.63 | -0.89 | -0.83 | -0.99 | -1.12 | -1.26 | -1.18 | 0.16 | -0.03 | -0.13 | 1.96 | 2.25 | 2.26 | -0.17 | -0.09 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | -1.1 | -2.7 | -3.2 | -3.8 | -5.5 | -5.6 | -6.5 | -7.5 | 0.9 | 2.0 | 2.3 | -3.1 | -2.7 | -1.2 |
Endoplasmic Reticulum | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 4.2 | 2.4 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7347 | 20.1046 | 19.8791 | 4.9689 | 10.8237 | 10.989 | 5.9827 | 8.4656 | 7.5686 | 2.4779 | 11.9007 | 7.4477 | 13.2493 | 20.4142 | 11.3757 | 7.8824 | 3.7724 | 11.5268 |
Actin | 0.0649 | 0.0011 | 0.0068 | 0.0053 | 0.0055 | 0.0058 | 0.0511 | 0.001 | 0.0115 | 0.0232 | 0.0049 | 0.0059 | 0.0705 | 0.0041 | 0.0061 | 0.0252 | 0.013 | 0.0166 |
Bud | 0.0019 | 0.0003 | 0.0052 | 0.051 | 0.0009 | 0.0015 | 0.0016 | 0.0081 | 0.0095 | 0.0392 | 0.0002 | 0.01 | 0.0017 | 0.0169 | 0.0207 | 0.009 | 0.0301 | 0.008 |
Bud Neck | 0.0007 | 0.0001 | 0.0003 | 0.0005 | 0.0004 | 0.0015 | 0.0052 | 0.0004 | 0.0003 | 0.0042 | 0.0005 | 0.0009 | 0.0112 | 0.0015 | 0.0026 | 0.0014 | 0.0006 | 0.0016 |
Bud Periphery | 0.0029 | 0.0004 | 0.0079 | 0.0141 | 0.0063 | 0.0007 | 0.0023 | 0.0037 | 0.0052 | 0.0261 | 0.0001 | 0.0061 | 0.0043 | 0.0099 | 0.0066 | 0.0035 | 0.0102 | 0.0045 |
Bud Site | 0.0018 | 0.0005 | 0.0055 | 0.0021 | 0.0002 | 0.0003 | 0.0101 | 0.0076 | 0.0081 | 0.0409 | 0.0009 | 0.0008 | 0.0147 | 0.0229 | 0.01 | 0.0022 | 0.0022 | 0.001 |
Cell Periphery | 0.0002 | 0.0003 | 0.0011 | 0.0004 | 0.0006 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.001 | 0.0001 | 0.0004 | 0.0017 | 0.0009 | 0.0021 | 0.0008 | 0.0003 | 0.0002 |
Cytoplasm | 0.2828 | 0.6569 | 0.6283 | 0.6811 | 0.6036 | 0.7026 | 0.4324 | 0.5104 | 0.5739 | 0.46 | 0.3013 | 0.582 | 0.4482 | 0.6678 | 0.6067 | 0.484 | 0.5397 | 0.5845 |
Cytoplasmic Foci | 0.0102 | 0.0038 | 0.0119 | 0.0131 | 0.0031 | 0.0185 | 0.0447 | 0.0267 | 0.014 | 0.0173 | 0.0229 | 0.0171 | 0.0441 | 0.0073 | 0.0276 | 0.0196 | 0.0084 | 0.0153 |
Eisosomes | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0018 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.013 | 0.0015 | 0.0112 | 0.0024 | 0.0378 | 0.0079 | 0.0041 | 0.003 | 0.0033 | 0.0021 | 0.0025 | 0.0031 | 0.01 | 0.0031 | 0.0065 | 0.0026 | 0.0031 | 0.003 |
Endosome | 0.0933 | 0.0006 | 0.0142 | 0.001 | 0.0069 | 0.0039 | 0.0335 | 0.0096 | 0.0021 | 0.0054 | 0.0324 | 0.0037 | 0.0316 | 0.0009 | 0.0073 | 0.0057 | 0.008 | 0.0079 |
Golgi | 0.0208 | 0.0002 | 0.0021 | 0.0002 | 0.0011 | 0.0005 | 0.0086 | 0.0023 | 0.0006 | 0.0025 | 0.0065 | 0.0008 | 0.0118 | 0.0003 | 0.0013 | 0.0025 | 0.001 | 0.002 |
Lipid Particles | 0.0108 | 0.0001 | 0.0039 | 0.0002 | 0.0002 | 0.0008 | 0.0172 | 0.0005 | 0.0006 | 0.0012 | 0.0077 | 0.0005 | 0.0306 | 0.0004 | 0.0013 | 0.0036 | 0.0016 | 0.0053 |
Mitochondria | 0.0184 | 0.0001 | 0.0174 | 0.0086 | 0.0154 | 0.0003 | 0.0028 | 0.0011 | 0.0008 | 0.0328 | 0.0007 | 0.001 | 0.0433 | 0.0014 | 0.0027 | 0.0055 | 0.0035 | 0.0021 |
None | 0.442 | 0.331 | 0.2615 | 0.1917 | 0.2902 | 0.2352 | 0.3469 | 0.4165 | 0.3501 | 0.3328 | 0.5623 | 0.3327 | 0.2003 | 0.2393 | 0.2689 | 0.3816 | 0.3373 | 0.318 |
Nuclear Periphery | 0.0158 | 0.0006 | 0.0051 | 0.0044 | 0.0106 | 0.0041 | 0.0041 | 0.0007 | 0.0016 | 0.001 | 0.0051 | 0.003 | 0.0158 | 0.0023 | 0.0039 | 0.0049 | 0.0087 | 0.0036 |
Nucleolus | 0.0003 | 0 | 0.0006 | 0.0011 | 0.0001 | 0.0001 | 0.002 | 0.0004 | 0.0005 | 0.0002 | 0.0017 | 0.0007 | 0.0111 | 0.0013 | 0.0018 | 0.002 | 0.0012 | 0.001 |
Nucleus | 0.0018 | 0.0012 | 0.0048 | 0.0142 | 0.0103 | 0.0105 | 0.0036 | 0.0018 | 0.0033 | 0.0033 | 0.006 | 0.0141 | 0.009 | 0.0107 | 0.0079 | 0.0198 | 0.0113 | 0.0084 |
Peroxisomes | 0.0061 | 0.0001 | 0.0014 | 0.0006 | 0 | 0.0002 | 0.017 | 0.0015 | 0.0072 | 0.0023 | 0.0029 | 0.0102 | 0.0222 | 0.0003 | 0.0023 | 0.0023 | 0.001 | 0.0044 |
Punctate Nuclear | 0.0027 | 0.0003 | 0.0077 | 0.0048 | 0.0003 | 0.0024 | 0.008 | 0.0031 | 0.0058 | 0.0016 | 0.0402 | 0.0043 | 0.0113 | 0.0066 | 0.008 | 0.0178 | 0.0164 | 0.0103 |
Vacuole | 0.0037 | 0.0005 | 0.0019 | 0.002 | 0.0033 | 0.0022 | 0.003 | 0.0012 | 0.001 | 0.002 | 0.0007 | 0.002 | 0.0034 | 0.0014 | 0.0043 | 0.0042 | 0.0018 | 0.0016 |
Vacuole Periphery | 0.0052 | 0.0001 | 0.0009 | 0.0008 | 0.0031 | 0.0008 | 0.0008 | 0.0001 | 0.0002 | 0.0006 | 0.0003 | 0.0005 | 0.0015 | 0.0002 | 0.0012 | 0.0015 | 0.0007 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.5224 | 24.9473 | 28.4799 | 28.9936 | 22.2663 | 12.1579 | 36.8661 | 37.978 | 35.6607 | 26.44 |
Translational Efficiency | 0.8781 | 0.8837 | 0.8018 | 0.8482 | 1.1276 | 0.8442 | 0.949 | 0.8055 | 0.7991 | 0.6677 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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