ORF | |
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Human Ortholog | |
Description | Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 0 | 0 | 1 | 4 |
Bud | 2 | 0 | 2 | 1 | 2 | 1 | 1 | 3 | 3 | 6 | 5 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 9 | 4 | 1 | 4 | 10 | 17 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 0 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 1 | 1 | 1 | 2 | 2 | 1 | 0 | 2 | 3 | 7 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 836 | 362 | 134 | 93 | 173 | 181 | 229 | 244 | 143 | 254 | 175 | 239 | 301 | 478 | 524 | 178 | 162 | 210 | 745 | 326 | 113 | 205 | 321 | 324 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 14 | 2 | 2 | 5 | 7 | 11 |
Golgi | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 0 | 0 | 1 | 3 | 2 |
Mitochondria | 13 | 4 | 6 | 3 | 2 | 2 | 3 | 4 | 10 | 16 | 14 | 18 | 1 | 1 | 0 | 0 | 0 | 0 | 7 | 2 | 0 | 0 | 3 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 1 | 3 | 0 | 1 | 1 | 1 | 1 | 2 | 3 | 2 | 0 | 2 | 2 | 2 |
Nuclear Periphery | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 6 | 2 | 0 | 0 | 3 | 5 |
Vac/Vac Membrane | 8 | 1 | 0 | 0 | 2 | 0 | 2 | 12 | 3 | 8 | 1 | 9 | 2 | 1 | 0 | 24 | 21 | 8 | 36 | 14 | 10 | 13 | 20 | 49 |
Unique Cell Count | 857 | 367 | 137 | 93 | 176 | 185 | 236 | 261 | 150 | 272 | 184 | 261 | 306 | 480 | 527 | 183 | 165 | 215 | 848 | 366 | 137 | 237 | 386 | 430 |
Labelled Cell Count | 866 | 370 | 143 | 97 | 182 | 188 | 242 | 267 | 161 | 289 | 198 | 282 | 310 | 481 | 529 | 208 | 192 | 231 | 848 | 366 | 137 | 237 | 386 | 430 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.1 | 3.9 | 4.4 | 6.7 | 6.0 | 6.4 | 6.5 | 5.6 | 6.3 | 5.7 | 6.1 | 5.8 | 6.7 | 6.5 | 25.5 | 26.4 | 23.7 | 5.7 | 7.1 | 7.4 |
Std Deviation (1e-4) | 1.0 | 1.0 | 1.4 | 1.3 | 1.4 | 1.3 | 1.8 | 1.7 | 1.9 | 2.2 | 1.9 | 2.0 | 1.1 | 1.4 | 1.2 | 4.6 | 4.5 | 3.9 | 1.4 | 2.9 | 2.5 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.78 | 0.62 | 0.72 | 0.74 | 0.53 | 0.68 | 0.54 | 0.63 | 0.57 | 0.77 | 0.72 | 2.7 | 2.75 | 2.6 | 0.54 | 0.86 | 0.93 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.4 | 0.3 | 0 | -0.4 | -1.9 | -1.1 | -1.9 | -1.3 | -2.4 | 0.4 | 2.0 | 1.8 | -0.3 | 0.2 | -0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0.8 | 0.6 | 1.2 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 4.3 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7186 | 2.0844 | 1.3743 | 0.9007 | 0.811 | 1.6663 | 1.0859 | 0.5552 | 0.7136 | 0.7185 | 0.8082 | 0.8451 | 1.9346 | 2.4569 | 2.0703 | 2.1618 | 1.8316 | 2.1623 |
Actin | 0.0193 | 0.0001 | 0.0003 | 0.0026 | 0.0003 | 0.0006 | 0.0457 | 0.0035 | 0.0227 | 0.0133 | 0.0627 | 0.0229 | 0.0034 | 0.0062 | 0.03 | 0.0001 | 0.0002 | 0.0077 |
Bud | 0.0002 | 0.0015 | 0.0013 | 0.0001 | 0.0001 | 0.0003 | 0.0023 | 0.0047 | 0.0009 | 0.0044 | 0.0122 | 0.0012 | 0.0001 | 0.0016 | 0.0008 | 0.0001 | 0.0002 | 0.0008 |
Bud Neck | 0.0014 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.002 | 0.0109 | 0.0002 | 0.0103 | 0.0005 | 0.0006 | 0.0073 | 0.0115 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0.0011 |
Bud Periphery | 0.0003 | 0.0014 | 0.0014 | 0 | 0 | 0.0002 | 0.0055 | 0.0063 | 0.001 | 0.0073 | 0.0205 | 0.0024 | 0.0002 | 0.0016 | 0.0013 | 0.0001 | 0.0001 | 0.0023 |
Bud Site | 0.0031 | 0.0012 | 0.0013 | 0 | 0.0001 | 0.0002 | 0.012 | 0.013 | 0.0032 | 0.0285 | 0.0036 | 0.0015 | 0.0103 | 0.0086 | 0.0024 | 0 | 0.0006 | 0.001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0008 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.515 | 0.6911 | 0.6535 | 0.7083 | 0.696 | 0.7006 | 0.2904 | 0.5164 | 0.4287 | 0.5431 | 0.4509 | 0.5249 | 0.3026 | 0.3854 | 0.3283 | 0.257 | 0.3498 | 0.4155 |
Cytoplasmic Foci | 0.0108 | 0.0057 | 0.0095 | 0.0087 | 0.0141 | 0.0162 | 0.0517 | 0.0098 | 0.0323 | 0.0155 | 0.0436 | 0.0205 | 0.0071 | 0.0051 | 0.0075 | 0.0043 | 0.0544 | 0.0173 |
Eisosomes | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0064 | 0.0019 | 0.0025 | 0.001 | 0.0062 | 0.0022 | 0.0064 | 0.0017 | 0.0046 | 0.0036 | 0.0043 | 0.0036 | 0.0013 | 0.0009 | 0.0014 | 0.0008 | 0.0007 | 0.0009 |
Endosome | 0.0096 | 0.0048 | 0.0056 | 0.0015 | 0.0118 | 0.009 | 0.0458 | 0.002 | 0.0395 | 0.0656 | 0.0501 | 0.0229 | 0.0059 | 0.001 | 0.0037 | 0.0009 | 0.0135 | 0.0074 |
Golgi | 0.0025 | 0.0001 | 0.0002 | 0.0006 | 0.0003 | 0.0007 | 0.0203 | 0.0004 | 0.0104 | 0.0129 | 0.0281 | 0.022 | 0.0011 | 0.0005 | 0.0018 | 0.0001 | 0.0019 | 0.0021 |
Lipid Particles | 0.0026 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0005 | 0.0255 | 0.0003 | 0.0028 | 0.0062 | 0.0057 | 0.007 | 0.0017 | 0.0004 | 0.0015 | 0.0001 | 0.0059 | 0.0032 |
Mitochondria | 0.0013 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0155 | 0.0004 | 0.0052 | 0.0651 | 0.0349 | 0.0128 | 0.0002 | 0.0019 | 0.0008 | 0.0001 | 0.0004 | 0.0044 |
None | 0.4085 | 0.2849 | 0.3138 | 0.272 | 0.2634 | 0.2594 | 0.4071 | 0.4348 | 0.4235 | 0.2081 | 0.2508 | 0.3316 | 0.6455 | 0.5788 | 0.6111 | 0.7341 | 0.5427 | 0.5157 |
Nuclear Periphery | 0.0042 | 0.0009 | 0.0014 | 0.0004 | 0.0018 | 0.001 | 0.0068 | 0.0006 | 0.0029 | 0.0031 | 0.0024 | 0.0016 | 0.0032 | 0.0003 | 0.0011 | 0.0003 | 0.0007 | 0.0023 |
Nucleolus | 0.0006 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0014 | 0.0001 | 0.0001 | 0.0008 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0007 | 0.0008 |
Nucleus | 0.0032 | 0.0016 | 0.0029 | 0.0017 | 0.0017 | 0.0029 | 0.0047 | 0.0014 | 0.0016 | 0.0029 | 0.0028 | 0.0017 | 0.0013 | 0.0011 | 0.002 | 0.0009 | 0.0022 | 0.0023 |
Peroxisomes | 0.0018 | 0 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0229 | 0.0013 | 0.0045 | 0.0013 | 0.0161 | 0.0097 | 0.0006 | 0.0047 | 0.0023 | 0.0001 | 0.0037 | 0.0038 |
Punctate Nuclear | 0.0062 | 0.0004 | 0.0008 | 0.0006 | 0.0005 | 0.0013 | 0.0123 | 0.0008 | 0.0012 | 0.0016 | 0.0025 | 0.0027 | 0.0027 | 0.0007 | 0.0019 | 0.0003 | 0.0199 | 0.0104 |
Vacuole | 0.0018 | 0.0036 | 0.0036 | 0.0005 | 0.0027 | 0.0021 | 0.0087 | 0.002 | 0.0034 | 0.0127 | 0.0057 | 0.0023 | 0.0007 | 0.0007 | 0.0009 | 0.0004 | 0.0017 | 0.0007 |
Vacuole Periphery | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0003 | 0.0029 | 0.0002 | 0.001 | 0.0025 | 0.0014 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.9871 | 2.8591 | 14.8508 | 20.6882 | 10.3668 | 15.9825 | 15.1767 | 32.193 | 21.3875 | 18.704 |
Translational Efficiency | 3.4494 | 8.8949 | 2.1 | 1.7933 | 2.1001 | 7.3047 | 3.9294 | 1.6122 | 2.471 | 2.07 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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