Standard name
Human Ortholog
Description Putative sterol transfer protein; localizes to puncta in the cortical ER; probable role in retrograde transport of sterols from the plasma membrane to the ER; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; contains GRAM, StART-like (VASt) and two PH-like domains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.19 0.06 0.14 0.14 0.13 0.17
Bud 0 0 0 0 0 0.05 0 0 0.05 0.05 0.05 0.1 0 0 0 0 0 0 0 0 0 0 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05 0.1 0 0.08 0.08 0.08 0.11 0.09 0.09 0.15
Cytoplasm 0.96 0.83 0.95 0.94 0.93 0.87 0.84 0.83 0.75 0.66 0.57 0.48 0.99 0.96 0.97 0.76 0.84 0.81 0.33 0.52 0.32 0.39 0.46 0.39
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0.05 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.21 0 0.12 0.08 0.26 0.27 0.25 0.42 0.49 0.58 0.65 0 0 0 0.07 0 0.07 0.2 0.19 0.3 0.23 0.09 0.16
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 0 0 0 0 0 0 0 0 0 0 1 1 5 8 51 3 39 11 19 21
Bud 0 1 2 5 3 14 14 4 17 20 10 29 0 0 5 1 4 6 3 0 0 0 8 1
Bud Neck 5 0 7 4 3 1 8 6 2 2 1 2 0 0 0 1 4 2 11 2 9 5 8 5
Bud Site 0 0 0 0 0 1 0 0 4 0 0 2 0 0 0 0 0 1 – – – – – –
Cell Periphery 5 0 4 3 5 3 5 7 9 12 4 4 8 5 7 15 6 12 22 4 31 6 13 19
Cytoplasm 239 39 259 167 271 270 349 335 263 256 120 133 120 136 143 109 130 127 91 29 89 30 70 50
Endoplasmic Reticulum 2 1 0 0 1 1 1 0 0 2 2 1 0 2 1 12 8 6 13 1 3 0 1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 4 0 4 1 4 1 4 2
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 4 0 0 0 1 0
Mitochondria 0 10 9 22 23 80 112 101 146 193 122 182 0 4 2 10 5 11 55 10 82 17 13 20
Nucleus 0 0 1 1 1 1 4 5 1 2 1 3 0 0 0 3 0 2 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 4 1 0 0 0 0 0
SpindlePole 2 0 2 1 4 3 4 4 9 6 1 6 0 0 0 3 6 5 3 0 1 0 1 0
Vac/Vac Membrane 0 0 0 0 1 0 0 0 0 1 1 0 0 2 1 5 4 3 7 1 5 1 3 1
Unique Cell Count 249 47 273 177 290 311 415 403 349 390 212 278 121 142 147 143 154 157 275 58 276 79 153 130
Labelled Cell Count 256 51 286 204 312 374 498 462 451 495 263 362 129 150 160 168 178 187 275 58 276 79 153 130


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 3.3 3.2 3.2 3.5 3.0 3.3 3.3 2.8 2.7 2.8 2.6 4.4 4.8 4.4 7.2 6.4 6.5 4.1 4.6 5.1
Std Deviation (1e-4) 0.9 0.6 0.6 1.4 1.3 0.8 1.7 1.5 1.5 1.2 1.3 1.3 2.2 2.5 2.5 4.6 3.0 3.5 1.3 2.1 2.2
Intensity Change (Log2) – – – 0.02 0.14 -0.09 0.03 0.04 -0.19 -0.22 -0.19 -0.27 0.47 0.6 0.47 1.17 1.0 1.04 0.37 0.51 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0054 0.0205 0.0204 0.0068 0.0573 0.0162 0.0573 0.0055 0.0156 0.0207 0.0066 0.0358 0.0001 0.0608 0.0729 0.1017 0.0229 0.0194
Bud 0.0001 0.0005 0.0004 0.0007 0.0004 0.0001 0.004 0.0013 0.0025 0.0005 0.0002 0.0003 0 0.0004 0.0022 0.0012 0.0004 0.0001
Bud Neck 0.0013 0.0042 0.0062 0.0062 0.0186 0.0356 0.0044 0.0039 0.0113 0.0141 0.0383 0.0201 0.0001 0.0018 0.0163 0.0026 0.0049 0.0286
Bud Periphery 0.0006 0.0017 0.0018 0.0035 0.0018 0.0007 0.0112 0.0026 0.0108 0.0013 0.0006 0.0027 0 0.0007 0.0052 0.0035 0.0009 0.0005
Bud Site 0.0026 0.0538 0.0194 0.0153 0.0026 0.0024 0.021 0.0851 0.0455 0.0054 0.0011 0.0009 0 0.02 0.0551 0.003 0.0018 0.0003
Cell Periphery 0.0227 0.0212 0.0117 0.0212 0.0126 0.0081 0.0055 0.0176 0.01 0.0101 0.0043 0.0072 0.0032 0.0039 0.0095 0.0058 0.002 0.004
Cytoplasm 0.4152 0.5109 0.4789 0.5598 0.4705 0.5644 0.1422 0.3163 0.3209 0.3319 0.3607 0.3641 0.0784 0.0966 0.2706 0.1764 0.2131 0.3503
Cytoplasmic Foci 0.0098 0.0107 0.0151 0.0456 0.0117 0.0106 0.0313 0.0102 0.0264 0.0373 0.0569 0.0333 0.003 0.0269 0.0189 0.0223 0.0387 0.0074
Eisosomes 0.0039 0.0021 0.001 0.0004 0.0015 0.0007 0.0022 0.0011 0.0012 0.0007 0.0007 0.0009 0.0006 0.0066 0.0026 0.0025 0.0026 0.0008
Endoplasmic Reticulum 0.0034 0.0039 0.0055 0.0034 0.0017 0.0037 0.0171 0.0019 0.0035 0.013 0.0013 0.0014 0.0012 0.0039 0.0048 0.0057 0.0024 0.0023
Endosome 0.0006 0.0013 0.0011 0.0004 0.0004 0.0012 0.0243 0.0004 0.0172 0.0301 0.0005 0.0102 0.0001 0.0051 0.0222 0.0091 0.0063 0.0006
Golgi 0.0006 0.0019 0.0014 0.0005 0.0007 0.0006 0.0154 0.0003 0.0098 0.0129 0.0008 0.0082 0 0.0076 0.0219 0.0074 0.0016 0.0004
Lipid Particles 0.0008 0.002 0.002 0.0003 0.0003 0.0024 0.0385 0.0005 0.0026 0.0177 0.0002 0.0053 0.0009 0.0021 0.0031 0.0032 0.0025 0.0004
Mitochondria 0.0002 0.0005 0.0003 0.0002 0.0002 0.0002 0.0116 0.0002 0.0094 0.0099 0.0002 0.003 0 0.0005 0.0239 0.0048 0.0008 0.0001
None 0.5304 0.3614 0.4314 0.3318 0.4185 0.351 0.5516 0.5505 0.5074 0.4329 0.5238 0.4875 0.9116 0.7392 0.457 0.6382 0.6881 0.5836
Nuclear Periphery 0.0002 0.0002 0.0003 0.0002 0.0001 0.0002 0.0047 0.0001 0.0005 0.0041 0.0001 0.0024 0 0.0017 0.0007 0.0012 0.0003 0.0001
Nucleolus 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0.0029 0.0002 0.0003 0.0003 0.0001 0.0002 0.0001 0.0006 0.0006 0.0004 0.0003 0.0001
Nucleus 0.0004 0.0006 0.0005 0.0005 0.0003 0.0004 0.0077 0.0005 0.0006 0.001 0.0003 0.0004 0 0.0015 0.0008 0.0012 0.0004 0.0002
Peroxisomes 0.0003 0.0008 0.0014 0.0024 0.0003 0.0005 0.0331 0.0005 0.0018 0.0495 0.0027 0.0078 0 0.0015 0.0055 0.0077 0.0023 0.0002
Punctate Nuclear 0.0004 0.0005 0.0004 0.0004 0.0002 0.0004 0.0076 0.0004 0.0005 0.0008 0.0003 0.008 0 0.0179 0.0008 0.0012 0.001 0.0002
Vacuole 0.0008 0.0011 0.0008 0.0004 0.0004 0.0006 0.0052 0.0008 0.0017 0.0041 0.0002 0.0004 0.0005 0.0005 0.0041 0.0008 0.0064 0.0005
Vacuole Periphery 0 0 0 0 0 0 0.001 0 0.0005 0.0017 0 0.0002 0 0.0001 0.0013 0.0002 0.0004 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.3102 10.7011 10.9185 19.0626 14.1626 12.8614 14.5392 18.1655 18.7739 21.8453
Translational Efficiency 0.3541 0.3522 0.4324 0.289 0.3369 0.3959 0.3759 0.2401 0.2532 0.2679

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1559 60 198 424 1465 1829 162 219 3024 1889 360 643

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 667.76 312.06 1028.97 847.46 676.22 706.12 929.21 859.98 671.86 693.60 984.08 851.72
Standard Deviation 692.11 10.51 125.18 116.80 66.93 92.44 87.17 97.46 499.14 114.25 120.42 110.75
Intensity Change Log 2 — -1.097506 0.623799 0.343816 — 0.062421 0.458512 0.346810 — -0.400519 0.543002 0.345323

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000150 0.000043 0.005990 0.010408 0.000904 0.002109 0.000354 0.013227 0.000515 0.002043 0.003454 0.011368
Bud Neck 0.006336 0.000019 0.005553 0.012048 0.030461 0.072803 0.003481 0.012869 0.018024 0.070492 0.004620 0.012328
Bud Site 0.003334 0.000037 0.035492 0.038064 0.005020 0.023086 0.003000 0.059198 0.004151 0.022353 0.020871 0.045262
Cell Periphery 0.000239 0.000092 0.001691 0.001160 0.000491 0.000710 0.000784 0.001551 0.000361 0.000690 0.001283 0.001293
Cytoplasm 0.596604* 0.000060 0.379999* 0.411334* 0.451870* 0.356571* 0.225021* 0.350369* 0.526486* 0.345247* 0.310259* 0.390570*
Cytoplasmic Foci 0.106539 0.000021 0.072072 0.063182 0.238864* 0.279193* 0.063561 0.076684 0.170644 0.270326* 0.068242 0.067780
Eisosomes 0.000172 0.000085 0.000433 0.000562 0.002229 0.001759 0.000322 0.000841 0.001168 0.001706 0.000383 0.000657
Endoplasmic Reticulum 0.000347 0.000001 0.015032 0.003406 0.001042 0.001102 0.006224 0.003912 0.000684 0.001067 0.011068 0.003578
Endosome 0.002024 0.000002 0.008233 0.014648 0.006627 0.025773 0.007293 0.004699 0.004254 0.024954 0.007810 0.011260
Golgi 0.000464 0.000008 0.000590 0.005741 0.006342 0.015716 0.000152 0.000404 0.003312 0.015217 0.000393 0.003923
Lipid Particles 0.003166 0.000039 0.005428 0.004416 0.013827 0.013552 0.007066 0.004330 0.008331 0.013123 0.006165 0.004386
Mitochondria 0.000851 0.000015 0.000199 0.011971 0.006126 0.018171 0.000222 0.000676 0.003407 0.017594 0.000209 0.008124
Mitotic Spindle 0.000095 0.000364 0.000514 0.021195 0.000661 0.005220 0.000374 0.005563 0.000369 0.005065 0.000451 0.015871
None 0.008758 0.999002* 0.000955 0.001478 0.001881 0.001661 0.001054 0.000935 0.005426 0.033340 0.000999 0.001293
Nuclear Periphery 0.000241 0.000000 0.000909 0.001551 0.000201 0.000236 0.001621 0.000615 0.000222 0.000228 0.001229 0.001232
Nuclear Periphery Foci 0.000554 0.000051 0.013424 0.002547 0.000172 0.000417 0.002750 0.001568 0.000369 0.000405 0.008621 0.002213
Nucleolus 0.000607 0.000045 0.000206 0.003580 0.000562 0.001663 0.000784 0.000149 0.000585 0.001612 0.000466 0.002412
Nucleus 0.253054* 0.000018 0.203252* 0.148185* 0.186062* 0.075188 0.513495* 0.178336* 0.220599* 0.072800 0.342862* 0.158454*
Peroxisomes 0.001212 0.000095 0.001170 0.006406 0.010871 0.031985 0.001377 0.010814 0.005891 0.030972 0.001263 0.007908
Vacuole 0.014609 0.000002 0.248612* 0.234184* 0.035335 0.071097 0.160875 0.273099* 0.024650 0.068839 0.209131* 0.247438*
Vacuole Periphery 0.000645 0.000000 0.000246 0.003934 0.000450 0.001987 0.000190 0.000161 0.000551 0.001924 0.000221 0.002649

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.02 -2.54 -2.97 -3.02 -0.74 -3.65 3.89 -2.35 -2.00 -2.48 -4.88 -2.29 -3.73 -2.90 -2.25
Bud Neck 6.15 0.67 -2.41 -8.34 -3.32 -11.60 12.84 8.08 18.39 -3.50 -16.59 10.91 5.38 19.47 -4.92
Bud Site 3.47 -5.60 -6.47 -7.42 -0.10 -12.26 2.08 -4.48 -2.23 -4.63 -12.31 -5.17 -7.73 -2.97 -3.40
Cell Periphery 6.53 -5.50 -3.12 -3.80 1.82 -2.79 -2.74 -3.49 -2.17 -1.80 -4.70 -5.98 -4.00 -1.78 0.86
Cytoplasm 110.66* 14.02 17.92 -23.60 0.59 11.16 16.73 8.59 3.14 -4.88 24.42 19.58 17.90 2.09 -2.68
Cytoplasmic Foci 35.50* 6.61 10.62 -13.34 1.36 -5.40 18.54 22.34 27.46 0.01 -16.21 18.67 24.70 34.55 1.07
Eisosomes 4.73 -3.46 -3.76 -4.78 -0.81 1.26 5.67 4.60 5.61 -2.73 -2.30 4.83 3.70 6.58 -2.20
Endoplasmic Reticulum 3.29 -7.99 -8.63 -9.57 5.56 -0.27 -7.16 -5.94 -5.68 2.63 -1.97 -9.81 -10.22 -8.48 6.04
Endosome 6.11 -8.03 -3.57 -4.61 -0.50 -13.02 -1.19 2.95 14.80 3.17 -15.47 -5.41 -2.35 9.20 0.46
Golgi 4.67 -0.09 -1.39 -1.66 -1.38 -8.01 10.38 10.13 15.31 -1.70 -11.66 9.71 1.18 8.94 -1.45
Lipid Particles 6.36 -3.34 -1.66 -8.70 1.64 0.25 5.17 8.74 8.80 3.81 -4.73 2.76 6.26 9.43 3.88
Mitochondria 3.54 2.77 -1.90 -2.22 -2.16 -6.40 5.71 5.34 11.16 -1.18 -8.82 6.19 -0.39 5.71 -2.20
Mitotic Spindle -4.22 -3.04 -3.99 -3.93 -3.90 -3.74 -0.15 -1.58 -0.67 -1.56 -4.08 -0.91 -4.23 -2.82 -4.16
None -997.52* 8.03 7.40 4467.70* -2.38 0.41 2.49 2.63 2.06 0.68 -6.84 8.35 7.63 7.96 -1.71
Nuclear Periphery 3.99 -7.88 -5.67 -6.79 -2.50 -0.46 -12.68 -8.15 -5.93 7.56 -0.09 -14.27 -6.86 -6.49 0.25
Nuclear Periphery Foci 1.29 -9.35 -3.31 -4.84 7.44 -1.45 -7.04 -7.53 -4.98 2.96 -0.15 -10.19 -4.84 -4.92 7.23
Nucleolus 4.83 3.45 -0.92 -1.27 -1.17 -2.20 -0.73 2.83 3.39 5.84 -2.25 1.41 -0.77 0.16 -0.92
Nucleus 53.00* 3.19 9.67 -17.84 2.91 21.20 -12.97 0.75 -7.52 11.81 34.61 -7.24 7.18 -11.58 10.16
Peroxisomes 4.04 0.66 -2.72 -3.96 -3.00 -11.20 9.24 1.79 9.34 -2.44 -14.70 8.96 0.84 13.56 -3.71
Vacuole 23.87 -15.83 -23.06 -24.10 -2.22 -10.84 -10.12 -16.65 -14.70 -5.44 -14.54 -18.37 -28.18 -23.72 -5.28
Vacuole Periphery 1.58 1.01 -1.62 -2.71 -2.40 -6.46 4.07 4.97 7.99 1.72 -4.46 1.58 -1.54 1.01 -2.36
Standard name
Human Ortholog
Description Putative sterol transfer protein; localizes to puncta in the cortical ER; probable role in retrograde transport of sterols from the plasma membrane to the ER; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; contains GRAM, StART-like (VASt) and two PH-like domains
Localization
Cell Percentages cytoplasm (43%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Lam1

Lam1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lam1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available