Standard name
Human Ortholog
Description Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.92 0.94 0.98 0.98 0.96 0.94 0.87 0.88 0.79 0.85 0.73 0.83 0.99 0.96 0.98 – – 0.2 0.78 0.74 0.71 0.82 0.82 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0.1 0.25 0.18 0.37 0.22 0.47 0.24 0 0 0 – – 0 0.08 0.08 0.13 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.49 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.32 0 0.06 0 0.07 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – 0 2 3 3 1 2 3
Bud 2 1 0 1 0 2 7 6 4 9 10 4 0 0 0 – – 0 0 1 1 0 3 5
Bud Neck 0 0 7 12 12 11 2 14 7 7 3 9 6 2 4 – – 0 1 2 1 0 1 3
Bud Site 0 0 0 0 0 0 0 0 0 3 3 1 0 0 0 – – 1 – – – – – –
Cell Periphery 1 1 2 2 1 1 0 2 3 3 1 0 0 1 1 – – 0 1 1 1 0 1 1
Cytoplasm 245 217 210 282 434 418 458 559 483 544 345 346 204 345 318 – – 34 213 183 145 109 215 254
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 – – 12 2 0 0 0 0 1
Endosome 0 0 1 0 1 1 0 1 0 1 1 0 0 0 0 – – 2 4 4 2 0 1 0
Golgi 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 – – 0 1 2 0 1 1 1
Mitochondria 31 5 0 4 4 46 131 114 225 142 224 101 0 0 0 – – 5 22 20 25 5 8 9
Nucleus 1 1 0 0 7 5 3 6 8 5 11 11 2 8 1 – – 82 2 7 2 1 6 2
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 – – 0 0 0 0 0 1 0
Nucleolus 1 0 1 0 1 0 0 1 0 0 0 0 0 0 0 – – 1 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 1 0 0 0 0 1
SpindlePole 0 0 0 1 6 4 6 11 7 13 5 10 0 0 2 – – 0 2 2 2 1 4 4
Vac/Vac Membrane 1 7 1 0 6 2 9 3 5 5 6 9 0 7 7 – – 53 11 14 5 8 8 10
Unique Cell Count 266 230 214 289 452 446 527 638 610 638 475 419 206 359 326 168 275 249 206 134 264 307
Labelled Cell Count 282 232 222 304 474 490 616 717 743 732 610 491 212 364 333 190 275 249 206 134 264 307


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 4.1 4.5 4.0 4.4 3.5 3.0 3.6 2.9 3.3 2.8 3.3 4.5 4.8 4.7 – – 6.0 4.6 4.7 5.2
Std Deviation (1e-4) 0.6 0.8 1.2 1.5 2.2 1.3 1.2 0.7 1.0 1.0 1.0 1.0 1.2 1.2 1.3 – – 1.6 1.8 1.5 2.0
Intensity Change (Log2) – – – -0.17 -0.05 -0.38 -0.58 -0.33 -0.65 -0.47 -0.71 -0.46 -0.01 0.07 0.04 – – 0.4 0.01 0.06 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 8.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.3374 -0.7705 -1.1155 -1.041 -1.6822 -0.8205 -0.849 -0.1344 -0.4042 -0.7555 -0.7885 -0.1895 -1.2013 -0.5094 -0.9023 -0.9296 -0.8457 -1.066
Actin 0.0006 0.0002 0.0002 0.0002 0.0004 0.0004 0.0002 0.0003 0.0005 0.0001 0.0001 0.0006 0.0083 0.0003 0.0009 0.0001 0.0005 0.0007
Bud 0.0008 0.0023 0.0002 0 0.0001 0.0002 0.0013 0.0054 0.0012 0 0.0002 0.0001 0.0001 0.0077 0.004 0 0.0014 0.0003
Bud Neck 0.0022 0.0002 0.0001 0.0001 0.0005 0.0006 0.0012 0.0004 0.0031 0.0004 0.0004 0.001 0.0005 0.0012 0.0002 0.0002 0.0003 0.0007
Bud Periphery 0.0004 0.0005 0.0001 0 0.0001 0.0003 0.0018 0.0011 0.0005 0 0.0001 0.0001 0.0003 0.0016 0.0009 0.0001 0.0008 0.0002
Bud Site 0.0009 0.0036 0.0002 0 0 0.0001 0.0014 0.0034 0.0004 0 0.0003 0.0001 0.0003 0.0076 0.001 0 0.0002 0.0001
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0.0001 0 0 0 0.0001 0.0002 0.0001 0 0.0002 0
Cytoplasm 0.0221 0.0274 0.03 0.061 0.0559 0.0398 0.0457 0.0552 0.0461 0.058 0.0196 0.068 0.0694 0.0836 0.0931 0.0596 0.1429 0.1247
Cytoplasmic Foci 0.0081 0.0026 0.0022 0.0017 0.0109 0.0066 0.0035 0.0025 0.0038 0.0062 0.0075 0.0042 0.0054 0.0079 0.0071 0.0048 0.0116 0.0071
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0005 0.0006 0.0004 0.0003 0.0012 0.0003 0.0003 0.0007 0.0004 0.0004 0.0002 0.0004 0.0008 0.0014 0.0008 0.0006 0.0019 0.0008
Endosome 0.0005 0.0013 0.0003 0.0005 0.001 0.0007 0.0003 0.0005 0.0004 0.0009 0.0002 0.0007 0.0028 0.0024 0.0024 0.0008 0.0031 0.0043
Golgi 0.0001 0.0001 0 0 0.0001 0 0 0.0001 0 0 0 0.0004 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001
Lipid Particles 0.0003 0.0002 0.0001 0.0001 0.0017 0.0002 0.0002 0.0002 0.0001 0.0003 0.0002 0.0005 0.0009 0.0002 0.0005 0.0006 0.002 0.0003
Mitochondria 0.0051 0.0003 0.0003 0.0002 0.0006 0.0002 0.0008 0.0004 0.0004 0.0002 0.0002 0.0007 0.0006 0.0004 0.001 0.0007 0.0014 0.0006
None 0.9275 0.9289 0.9173 0.9087 0.8671 0.8874 0.8741 0.8762 0.8808 0.7988 0.8589 0.8007 0.8844 0.8465 0.8475 0.9229 0.7732 0.7933
Nuclear Periphery 0.0011 0.004 0.0039 0.0017 0.0074 0.0015 0.0008 0.0022 0.0012 0.0016 0.0011 0.0024 0.0015 0.0014 0.0019 0.0011 0.004 0.002
Nucleolus 0.0008 0.0015 0.0009 0.0009 0.0008 0.0011 0.0012 0.0015 0.0013 0.001 0.0023 0.002 0.002 0.001 0.0012 0.0006 0.0015 0.0008
Nucleus 0.0242 0.0178 0.0314 0.0196 0.0459 0.0448 0.0594 0.031 0.0432 0.0677 0.0585 0.0837 0.0148 0.0243 0.0291 0.003 0.0423 0.0562
Peroxisomes 0.0002 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0036 0.001 0.0002 0.0006 0.0003 0.0006 0.0003
Punctate Nuclear 0.0039 0.0077 0.0118 0.0044 0.0057 0.0151 0.0068 0.0183 0.0158 0.0637 0.0497 0.0305 0.0044 0.0097 0.006 0.0037 0.0086 0.0064
Vacuole 0.0003 0.0004 0.0002 0.0002 0.0003 0.0003 0.0004 0.0004 0.0003 0.0002 0.0002 0.0002 0.0016 0.0018 0.001 0.0004 0.0025 0.0009
Vacuole Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0002 0.0002 0.0002 0.0001 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.3144 9.642 6.4393 5.2327 4.1953 3.234 4.3871 6.2509 4.2933 5.6197
Translational Efficiency 1.8618 0.6028 1.0506 1.374 1.3669 3.3388 1.4144 0.8994 1.694 1.2577

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1826 1229 2110 504 1474 2135 1623 1551 3300 3364 3733 2055

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 646.99 711.52 723.64 830.76 605.02 687.10 788.87 1221.24 628.24 696.02 752.00 1125.47
Standard Deviation 67.47 76.49 87.59 95.40 68.62 84.95 94.59 1279.35 71.12 82.80 96.29 1125.06
Intensity Change Log 2 — 0.137161 0.161529 0.360688 — 0.183537 0.382805 1.013292 — 0.159758 0.272699 0.712785

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000096 0.000385 0.000124 0.000320 0.000077 0.000361 0.000110 0.000757 0.000088 0.000369 0.000118 0.000650
Bud Neck 0.018106 0.036376 0.011385 0.005565 0.009433 0.040880 0.003039 0.013314 0.014232 0.039234 0.007756 0.011413
Bud Site 0.005642 0.013876 0.010368 0.004837 0.001846 0.013898 0.001650 0.024919 0.003947 0.013890 0.006578 0.019993
Cell Periphery 0.000161 0.000146 0.000179 0.000128 0.000110 0.000154 0.000132 0.000427 0.000138 0.000151 0.000159 0.000353
Cytoplasm 0.427172* 0.338942* 0.311561* 0.205830* 0.461803* 0.420029* 0.206399* 0.323930* 0.442640* 0.390405* 0.265840* 0.294966*
Cytoplasmic Foci 0.239900* 0.266505* 0.006945 0.003348 0.185209* 0.194065* 0.007794 0.008966 0.215471* 0.220530* 0.007315 0.007588
Eisosomes 0.000189 0.000146 0.000023 0.000039 0.000115 0.000121 0.000033 0.000031 0.000156 0.000130 0.000027 0.000033
Endoplasmic Reticulum 0.000828 0.001143 0.001088 0.006141 0.000736 0.000526 0.001692 0.002965 0.000787 0.000751 0.001350 0.003744
Endosome 0.008611 0.018013 0.001130 0.004166 0.005676 0.014440 0.001030 0.004756 0.007300 0.015746 0.001086 0.004611
Golgi 0.002356 0.006293 0.000078 0.000201 0.001386 0.003859 0.000066 0.004160 0.001923 0.004748 0.000072 0.003189
Lipid Particles 0.012667 0.010621 0.000640 0.001027 0.008473 0.005123 0.000986 0.001600 0.010794 0.007132 0.000790 0.001460
Mitochondria 0.009465 0.021110 0.001423 0.000859 0.003148 0.012092 0.000502 0.007156 0.006643 0.015387 0.001023 0.005611
Mitotic Spindle 0.001352 0.004471 0.009442 0.013313 0.000468 0.010167 0.002206 0.028559 0.000957 0.008086 0.006296 0.024819
None 0.003134 0.002765 0.007106 0.011288 0.003774 0.004028 0.011608 0.014159 0.003420 0.003567 0.009064 0.013455
Nuclear Periphery 0.000546 0.000678 0.002043 0.002517 0.000567 0.000431 0.001486 0.002361 0.000555 0.000521 0.001801 0.002399
Nuclear Periphery Foci 0.000280 0.000278 0.000161 0.000705 0.000298 0.000269 0.000302 0.000686 0.000288 0.000273 0.000222 0.000691
Nucleolus 0.001399 0.002514 0.001212 0.000849 0.001390 0.002295 0.000962 0.000833 0.001395 0.002375 0.001104 0.000837
Nucleus 0.229256* 0.189979* 0.617835* 0.686862* 0.284732* 0.215484* 0.743438* 0.523383* 0.254035* 0.206166* 0.672443* 0.563477*
Peroxisomes 0.007234 0.025109 0.000284 0.000155 0.004875 0.016203 0.000273 0.001176 0.006180 0.019457 0.000279 0.000926
Vacuole 0.030722 0.058503 0.016206 0.051262 0.025156 0.044098 0.016189 0.031784 0.028236 0.049361 0.016199 0.036561
Vacuole Periphery 0.000886 0.002147 0.000768 0.000589 0.000731 0.001476 0.000101 0.004078 0.000817 0.001721 0.000478 0.003222

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.62 -2.64 -9.48 -2.76 -8.51 -5.78 -4.88 -8.71 -4.02 -7.62 -8.13 -5.15 -10.54 -4.64 -9.47
Bud Neck -6.69 3.98 6.19 10.82 2.60 -14.25 6.11 -3.12 11.77 -8.24 -14.16 6.11 1.75 15.09 -3.90
Bud Site -4.83 -2.70 -0.12 4.33 2.31 -10.66 0.13 -9.27 -1.69 -9.30 -9.87 -2.65 -7.69 -0.37 -5.74
Cell Periphery 1.11 -0.02 2.47 1.66 2.23 -4.30 -1.80 -11.51 -9.37 -10.58 -1.39 -0.74 -9.74 -9.12 -9.10
Cytoplasm 9.29 17.22 28.97 18.26 14.97 4.61 32.27 19.14 15.61 -9.82 8.09 33.31 27.37 19.79 0.28
Cytoplasmic Foci -3.06 46.04 47.09 39.08 5.53 -1.15 34.19 34.16 40.10 -0.56 -0.81 56.80 56.85 54.90 0.57
Eisosomes 4.69 24.35 15.84 10.30 -9.67 -0.83 15.24 14.81 16.13 -1.13 4.64 28.45 23.76 19.94 -8.45
Endoplasmic Reticulum -1.61 -4.32 -12.55 -11.99 -11.83 1.15 -8.43 -14.28 -17.73 -8.34 0.22 -9.21 -19.44 -20.27 -15.75
Endosome -6.40 10.64 5.80 10.33 -1.63 -7.62 9.41 5.22 10.84 -2.87 -9.10 13.87 8.47 15.07 -3.32
Golgi -5.90 9.95 9.63 9.98 -0.96 -4.52 4.16 -0.29 3.45 -3.12 -6.92 9.66 1.69 6.81 -3.14
Lipid Particles 1.74 16.21 15.08 11.35 -4.78 4.53 10.72 9.67 8.99 -1.12 5.83 19.35 17.13 13.93 -2.60
Mitochondria -4.73 6.52 6.89 9.57 2.52 -7.46 5.03 -0.69 5.92 -3.79 -6.89 7.75 3.59 9.96 -3.18
Mitotic Spindle -2.89 -5.22 -3.30 -2.40 -1.07 -6.14 -3.12 -8.78 -4.20 -6.80 -6.47 -6.09 -9.14 -5.23 -5.88
None 0.69 -5.34 -3.85 -4.10 -0.43 -0.37 -4.08 -8.32 -7.76 -0.62 -0.34 -6.16 -10.04 -9.23 -2.12
Nuclear Periphery -2.28 -20.99 -15.71 -14.81 -6.89 3.05 -18.82 -11.12 -11.64 -5.68 0.57 -27.77 -14.94 -15.04 -6.60
Nuclear Periphery Foci -0.48 2.04 -6.16 -5.96 -6.71 1.04 0.35 -2.04 -9.18 -7.09 0.91 0.83 -5.81 -10.87 -11.11
Nucleolus -3.15 0.44 2.73 6.00 2.91 -2.03 2.82 3.04 4.16 0.25 -3.34 1.83 3.74 6.37 2.45
Nucleus 4.66 -50.77 -35.55 -36.58 -3.98 8.84 -54.31 -26.80 -35.41 18.56 8.56 -72.42 -42.12 -47.60 11.33
Peroxisomes -8.53 12.14 12.38 12.46 1.82 -8.32 7.38 6.65 12.92 -0.88 -11.58 13.97 12.68 17.79 -0.76
Vacuole -9.53 4.83 -9.80 -6.05 -10.86 -9.23 -0.16 -12.34 -6.94 -11.82 -12.77 3.46 -15.36 -8.63 -16.52
Vacuole Periphery -4.72 0.87 4.48 6.70 1.37 -2.73 3.91 -1.40 -0.21 -2.50 -4.82 2.40 -0.99 0.90 -1.80
Standard name
Human Ortholog
Description Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
Localization
Cell Percentages cytoplasm (13%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Lin1

Lin1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lin1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available