Standard name
Human Ortholog
Description Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on fermentable carbon sources, and heterodimerizes with Mpc1p to form the fermentative isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC2 paralog, MPC3, heterodimerizes with Mpc1p to form the respiratory MPC isoform
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.12 – 0.23 0 0 0 0 0.05 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.12 – 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0.07 0.11 0 0 0.05 0 – 0 0 0 0 0 0.09 0.1 0.17 0.73 – 0.55 0.08 0.14 0.09 0.11 0.15 0.18
Mitochondria 0.88 0.93 0.97 0.92 0.92 0.9 – 0.88 0.92 0.91 0.92 0.93 0.91 0.91 0.88 0 – 0.05 0.78 0.74 0.76 0.73 0.63 0.58
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0 0 0.09 – 0.09 0.12 0.1 0.07 0.07 0 0 0 0 – 0.05 0 0 0 0 0 0
Nucleolus 0.08 0.06 0.07 0.11 0.08 0.11 – 0.15 0.16 0.1 0.16 0.13 0.05 0.05 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.1 0.07 0 0.06 0 – 0.05 0.06 0 0.07 0 0.1 0.09 0.11 0.15 – 0.18 0.06 0.05 0 0.05 0.06 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 5 0 0 1 0 – 2 0 0 0 0 3 3 2 3 – 5 4 5 3 1 15 11
Bud 1 0 2 2 5 4 – 6 13 7 6 8 3 0 1 0 – 1 1 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 3 4 1 2 9 0 1 0 0 – 0 – – – – – –
Cell Periphery 5 7 1 1 0 4 – 2 3 3 3 6 4 4 3 3 – 1 1 0 0 0 0 0
Cytoplasm 3 2 2 3 2 4 – 4 1 4 1 4 2 10 9 0 – 4 0 0 0 0 1 1
Endoplasmic Reticulum 4 2 2 0 1 2 – 1 1 1 4 1 2 1 0 1 – 0 0 1 0 0 1 1
Endosome 0 0 0 0 1 0 – 5 0 1 0 0 0 1 1 0 – 0 4 0 1 1 4 5
Golgi 15 19 2 0 7 4 – 1 2 1 1 0 21 31 52 19 – 12 20 27 7 11 45 50
Mitochondria 198 164 69 78 142 223 – 497 280 303 203 307 203 284 266 1 – 1 187 150 63 70 190 162
Nucleus 1 0 0 0 0 2 – 4 2 1 0 1 1 0 1 0 – 0 0 0 0 0 1 2
Nuclear Periphery 8 5 4 3 1 22 – 52 37 33 15 24 6 9 5 0 – 1 0 0 1 0 3 2
Nucleolus 17 10 5 9 12 28 – 85 49 35 36 43 12 15 11 0 – 0 4 0 0 2 3 5
Peroxisomes 0 0 0 1 5 0 – 4 0 3 0 2 0 4 1 0 – 0 1 3 3 3 10 5
SpindlePole 0 0 0 0 0 0 – 4 0 1 3 2 1 1 1 0 – 0 0 0 0 0 1 2
Vac/Vac Membrane 36 17 5 3 9 9 – 31 17 14 15 12 22 29 33 4 – 4 14 10 3 4 18 25
Unique Cell Count 226 177 71 85 154 248 564 304 334 220 330 224 313 304 26 22 242 203 83 96 302 280
Labelled Cell Count 291 231 92 100 186 302 701 409 408 289 419 280 393 386 31 29 242 203 83 96 302 280


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.2 16.8 10.1 14.7 16.6 14.0 – 12.1 10.4 10.8 10.4 10.0 17.6 18.6 18.2 24.1 – 18.9 16.3 20.5 20.4
Std Deviation (1e-4) 3.7 3.5 2.4 3.0 4.3 3.9 – 3.4 3.3 3.3 3.4 3.3 3.8 4.0 4.1 5.3 – 5.7 3.9 5.3 5.3
Intensity Change (Log2) – – – 0.54 0.72 0.47 – 0.26 0.05 0.1 0.04 -0.01 0.8 0.89 0.85 1.26 – 0.9 0.69 1.02 1.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Cytoplasm 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 – 0 0 0 0 0 1.8 1.9 3.1 7.4 – 0
Mitochondria -1.4 -1.4 -1.9 – -2.3 -1.5 -1.8 -1.5 -1.3 -1.8 -1.8 -2.4 -9.1 – -8.8
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0.9 – 1.0 1.6 1.1 0 0 0 0 0 0 – 0
Nucleolus 0.8 0.2 1.0 – 1.8 2.0 0.9 2.0 1.4 0 0 0 0 – 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 0 0 – 0 0 0 0 0 0.7 0.6 1.0 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 42.0252 49.4774 43.5904 42.0751 43.9933 49.9574 45.0242 47.6393 44.435 39.5453 30.0303 46.026 47.5615 54.1969 46.5021 47.057 42.9649 52.9088
Actin 0.0196 0.0007 0.0196 0 0.0019 0.0001 0.0038 0.0011 0.0029 0.0027 0.0015 0.0011 0.0147 0.0011 0.0101 0.0001 0.0041 0.0001
Bud 0.0002 0.0001 0.0001 0 0.0003 0 0.0009 0.0001 0 0.0003 0.0001 0 0.0001 0 0.0004 0 0.0002 0
Bud Neck 0.0001 0 0.0008 0 0 0 0.0002 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0 0 0
Bud Periphery 0.0004 0.0001 0.0002 0 0.001 0 0.0013 0.0003 0.0001 0.0008 0.0001 0 0.0003 0.0001 0.0006 0 0.0005 0
Bud Site 0.0007 0 0.0005 0 0.0001 0 0.0028 0.001 0 0.0004 0.0002 0 0.0002 0.0002 0.0024 0.0001 0.0003 0
Cell Periphery 0.0003 0.0005 0.0002 0.0001 0.0001 0 0.0003 0.001 0.0002 0.0004 0.0003 0 0.0003 0.0005 0.0004 0.0001 0.0001 0
Cytoplasm 0.0002 0 0 0 0 0 0.0009 0 0 0 0.0005 0.0009 0.0012 0 0.0007 0.0003 0.0001 0
Cytoplasmic Foci 0.0046 0.0001 0.0042 0 0.0002 0 0.0027 0.0001 0.0001 0.0056 0.0093 0.0004 0.0021 0.0002 0.012 0.0215 0.002 0
Eisosomes 0.0008 0.0005 0.0007 0.0001 0.0003 0.0002 0.0003 0.0004 0.0018 0.0006 0.0012 0.0001 0.0021 0.0004 0.0005 0.0003 0.0002 0.0001
Endoplasmic Reticulum 0.0003 0 0 0 0 0 0.0006 0 0 0 0.0002 0.0024 0.0011 0 0.0009 0 0 0
Endosome 0.0026 0.0001 0.0013 0 0.0002 0 0.0018 0.0002 0.0007 0.0026 0.0183 0.0063 0.0048 0.0002 0.007 0.0002 0.0014 0
Golgi 0.004 0.0004 0.0044 0 0.0007 0.0001 0.0073 0.0005 0.0063 0.0099 0.022 0.0014 0.0088 0.0005 0.0032 0.0001 0.0019 0.0002
Lipid Particles 0.022 0.0011 0.0071 0.0005 0.002 0.0008 0.0044 0.0063 0.0083 0.073 0.0383 0.0082 0.0148 0.0091 0.0155 0.0089 0.0076 0.0048
Mitochondria 0.9097 0.9704 0.9404 0.9886 0.989 0.9913 0.9549 0.9742 0.9651 0.869 0.8907 0.9375 0.9046 0.9581 0.8994 0.9538 0.9586 0.9848
None 0.0011 0 0.0001 0 0 0 0.0004 0 0 0.0001 0.0013 0.0001 0.007 0 0.0009 0.0029 0.0003 0
Nuclear Periphery 0.0021 0 0 0 0 0.0001 0.0018 0 0.0001 0.0001 0.0002 0.0258 0.0039 0 0.0039 0.0001 0.0001 0.0001
Nucleolus 0.0093 0.0003 0.0032 0 0.0005 0 0.0038 0.002 0.0003 0.0015 0.0066 0.0001 0.0012 0.0066 0.0024 0.0041 0.0002 0.0001
Nucleus 0.0012 0.0002 0.0002 0 0.0002 0 0.0007 0.0002 0.0001 0.0003 0.0004 0.0016 0.0009 0.0002 0.0013 0.0004 0.0001 0
Peroxisomes 0.0074 0.001 0.0074 0.0015 0.0019 0.0002 0.0027 0.0017 0.001 0.0194 0.0035 0.0003 0.0018 0.0018 0.0113 0.0019 0.0193 0.0002
Punctate Nuclear 0.0005 0 0.0001 0 0 0 0.002 0 0 0.0002 0.0001 0.0013 0.0048 0 0.0034 0.0006 0.0002 0
Vacuole 0.0018 0.0071 0.0018 0.0005 0.0005 0.0001 0.0016 0.0022 0.0015 0.0034 0.0011 0.0003 0.0023 0.0037 0.0029 0.0008 0.0007 0.0002
Vacuole Periphery 0.0111 0.0175 0.0078 0.0084 0.001 0.0071 0.005 0.0082 0.0116 0.0097 0.004 0.0118 0.023 0.0171 0.0205 0.0038 0.002 0.0093

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 488.0443 376.8463 386.6111 305.0189 313.6342 653.1931 503.7764 364.1787 462.1821 342.0474
Translational Efficiency 0.9668 1.0883 0.8428 1.4598 1.1513 0.8332 0.7789 1.1385 0.81 0.8428

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1760 1077 1249 488 638 1503 1288 1399 2398 2580 2537 1887

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1178.10 1660.29 1262.52 1366.86 1363.07 1529.32 1463.95 1267.46 1227.31 1583.99 1364.78 1293.17
Standard Deviation 244.47 428.95 225.87 219.45 388.32 413.21 232.08 178.76 301.10 424.79 250.20 195.04
Intensity Change Log 2 — 0.494973 0.099844 0.214403 — 0.166031 0.103007 -0.104919 — 0.327887 0.101541 0.051938

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001232 0.004461 0.003182 0.003273 0.005025 0.004480 0.002760 0.001013 0.002241 0.004472 0.002968 0.001598
Bud Neck 0.000376 0.001008 0.001629 0.002564 0.001871 0.001441 0.001969 0.001154 0.000773 0.001260 0.001801 0.001519
Bud Site 0.000635 0.001023 0.004475 0.010416 0.004619 0.001544 0.002904 0.006777 0.001695 0.001326 0.003677 0.007718
Cell Periphery 0.000081 0.000107 0.000223 0.000486 0.001011 0.000150 0.000155 0.000590 0.000328 0.000132 0.000188 0.000563
Cytoplasm 0.000154 0.000189 0.000507 0.002657 0.001943 0.000237 0.000403 0.000681 0.000630 0.000217 0.000454 0.001192
Cytoplasmic Foci 0.006677 0.015284 0.015236 0.013453 0.013990 0.012817 0.011265 0.005533 0.008623 0.013847 0.013220 0.007581
Eisosomes 0.000136 0.000792 0.000132 0.000123 0.001392 0.000747 0.000126 0.000056 0.000470 0.000766 0.000129 0.000073
Endoplasmic Reticulum 0.000097 0.000149 0.000294 0.000530 0.000405 0.000232 0.000137 0.000469 0.000179 0.000197 0.000215 0.000485
Endosome 0.002238 0.002166 0.009892 0.016913 0.002386 0.003474 0.005375 0.009825 0.002277 0.002928 0.007599 0.011658
Golgi 0.011754 0.023581 0.033191 0.069100 0.013034 0.018706 0.014230 0.041586 0.012095 0.020741 0.023565 0.048701
Lipid Particles 0.003614 0.002898 0.002570 0.001460 0.005387 0.005561 0.002014 0.000769 0.004086 0.004449 0.002288 0.000947
Mitochondria 0.958575* 0.924418* 0.883574* 0.815221* 0.905194* 0.923413* 0.925374* 0.878833* 0.944373* 0.923832* 0.904795* 0.862382*
Mitotic Spindle 0.000395 0.000568 0.004265 0.008038 0.001526 0.000321 0.005732 0.002917 0.000696 0.000424 0.005010 0.004241
None 0.001109 0.002296 0.001025 0.000993 0.004888 0.002024 0.000887 0.000854 0.002115 0.002138 0.000955 0.000890
Nuclear Periphery 0.000172 0.000155 0.000512 0.000745 0.000351 0.000202 0.000719 0.000488 0.000220 0.000183 0.000617 0.000555
Nuclear Periphery Foci 0.000144 0.000156 0.000417 0.000558 0.000667 0.000219 0.000334 0.000244 0.000283 0.000193 0.000375 0.000325
Nucleolus 0.000170 0.000616 0.000643 0.000147 0.005237 0.000764 0.000562 0.000077 0.001518 0.000702 0.000602 0.000095
Nucleus 0.000047 0.000164 0.000222 0.000072 0.001872 0.000159 0.000087 0.000057 0.000533 0.000161 0.000154 0.000061
Peroxisomes 0.003755 0.006660 0.008937 0.008822 0.010081 0.007581 0.007161 0.001558 0.005438 0.007196 0.008036 0.003437
Vacuole 0.003517 0.006595 0.010345 0.018738 0.006697 0.007364 0.007819 0.015920 0.004363 0.007043 0.009062 0.016649
Vacuole Periphery 0.005121 0.006714 0.018729 0.025690 0.012424 0.008565 0.009988 0.030598 0.007064 0.007792 0.014291 0.029328

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.17 -3.50 -3.32 1.06 -0.13 0.30 1.23 2.30 5.02 2.65 -2.95 -1.12 1.18 4.81 3.06
Bud Neck -3.70 -6.05 -3.43 -2.39 -1.42 0.94 -0.21 1.54 0.84 1.97 -2.71 -4.90 -2.64 -0.83 0.85
Bud Site -1.06 -3.54 -3.55 -3.40 -2.02 1.86 1.03 -0.98 -3.58 -2.49 0.66 -2.46 -4.52 -4.98 -2.89
Cell Periphery -1.55 -3.02 -4.87 -4.53 -2.78 2.63 2.61 1.25 -4.60 -4.34 2.21 1.52 -2.06 -5.86 -4.80
Cytoplasm -0.72 -3.63 -1.55 -1.53 -1.34 1.45 1.34 1.13 -2.75 -1.68 1.33 0.70 -0.84 -2.23 -1.70
Cytoplasmic Foci -4.73 -5.35 -3.79 0.77 0.81 0.60 1.38 4.67 5.37 4.08 -4.51 -4.03 1.05 5.22 4.77
Eisosomes -10.43 0.21 0.71 10.59 0.56 4.16 8.99 9.53 10.45 4.49 -4.86 8.32 9.83 14.88 5.26
Endoplasmic Reticulum -1.19 -4.27 -4.60 -3.65 -2.30 2.36 3.71 -0.47 -2.55 -3.67 -0.47 -0.94 -4.26 -3.89 -3.73
Endosome 0.16 -7.37 -7.05 -7.08 -3.08 -1.44 -3.29 -6.35 -5.46 -3.52 -1.50 -7.93 -9.59 -8.76 -3.63
Golgi -4.09 -7.30 -7.76 -5.93 -4.66 -1.95 -0.44 -6.95 -5.95 -7.38 -4.53 -6.02 -10.83 -8.02 -7.20
Lipid Particles 0.95 1.25 2.90 2.42 1.58 -0.11 2.56 3.69 4.58 2.66 -0.44 2.71 5.39 5.40 3.43
Mitochondria 5.55 10.71 11.03 7.96 4.85 -2.04 -2.25 2.43 5.59 5.79 4.30 7.74 12.33 9.03 6.03
Mitotic Spindle -0.75 -6.20 -5.42 -5.22 -2.46 1.34 -3.01 -1.11 -4.72 2.63 1.00 -6.93 -5.73 -6.84 0.96
None -7.95 0.96 1.06 8.18 0.31 8.12 11.70 11.78 11.15 0.62 -0.14 10.23 10.69 14.25 1.31
Nuclear Periphery 0.53 -4.11 -2.47 -2.56 -0.96 2.05 -2.25 -0.96 -2.33 1.22 1.20 -4.51 -3.01 -3.43 0.45
Nuclear Periphery Foci -0.39 -3.07 -2.48 -2.34 -0.76 1.86 1.42 1.77 -0.48 1.58 1.31 -0.93 -0.36 -2.32 0.71
Nucleolus -2.16 -1.19 0.40 2.31 1.25 2.42 2.53 2.80 3.13 2.39 1.60 1.73 2.88 3.96 2.30
Nucleus -1.09 -1.49 -1.38 0.88 1.27 2.16 2.25 2.28 2.06 1.07 1.73 1.78 2.22 1.82 1.53
Peroxisomes -2.36 -3.26 -2.45 -0.98 0.05 1.06 1.22 3.90 5.35 4.87 -1.60 -2.17 2.10 3.99 4.35
Vacuole -2.23 -5.23 -5.04 -3.77 -2.63 -0.51 -0.83 -4.92 -4.63 -4.47 -2.99 -5.30 -8.27 -6.02 -4.81
Vacuole Periphery -1.56 -7.46 -6.80 -6.17 -2.03 1.88 1.21 -6.35 -9.01 -8.55 -0.78 -6.20 -11.48 -11.07 -7.28
Standard name
Human Ortholog
Description Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on fermentable carbon sources, and heterodimerizes with Mpc1p to form the fermentative isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC2 paralog, MPC3, heterodimerizes with Mpc1p to form the respiratory MPC isoform
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Mpc2

Mpc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mpc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available