Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0.06 0.07 0.06 – 0.07 0.09 0 0.06 0.12 0.14 0.2 0.08 0.11 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.16 0.14 – 0.36 0.29 0.68 0.41 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.93 0.9 0.96 0.87 0.88 0.74 0.8 – 0.79 0.68 0.73 0.72 0.88 0.85 0.78 0.73 0.71 0.77 0.87 0.89 0.73 0.73 0.76 0.65
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.08 0 – 0.05 0.05 0.08 0.05 0 0 0 0.05 0.08 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.12 0 0 0.05 0.07 0 – 0 0.06 0 0 0 0 0 0.14 0.12 0.07 0 0.05 0.16 0.12 0.1 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 0 – 5 4 0 2 0 1 0 0 0 0 2 1 0 0 1 2
Bud Neck 0 0 0 0 0 0 1 – 2 2 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 2 4 – 3 9 10 13 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 0 14 12 16 24 – 14 26 6 20 8 21 49 23 27 15 0 0 2 0 1 7
Endoplasmic Reticulum 0 0 0 1 0 0 0 – 0 0 0 0 0 1 1 8 4 3 0 0 0 0 0 1
Endosome 0 2 0 0 1 2 8 – 0 0 0 1 0 0 0 7 7 6 1 2 1 1 3 4
Golgi 0 0 0 1 0 0 0 – 0 0 0 0 0 0 1 4 2 1 1 1 0 0 0 0
Mitochondria 15 1 0 5 3 38 55 – 69 80 179 131 0 0 0 12 11 5 0 1 0 2 2 5
Nucleus 174 207 22 151 173 174 324 – 154 191 191 232 56 129 190 207 168 129 178 204 52 37 145 136
Nuclear Periphery 0 0 0 0 0 0 0 – 1 1 1 3 0 0 1 0 1 0 0 0 0 0 0 0
Nucleolus 4 3 0 1 4 19 17 – 9 15 21 15 0 1 2 13 18 12 6 4 0 0 7 7
Peroxisomes 0 0 0 0 0 0 0 – 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 2 8 10 – 5 6 3 7 0 2 3 3 2 4 2 1 0 2 8 2
Vac/Vac Membrane 9 27 1 7 9 17 12 – 6 17 6 14 1 0 4 39 29 11 8 11 11 5 18 36
Unique Cell Count 188 230 23 173 197 234 405 194 279 263 323 64 152 245 284 238 167 205 230 73 51 191 209
Labelled Cell Count 205 247 23 182 204 276 455 270 351 417 439 65 155 252 316 269 186 205 230 73 51 191 209


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.8 6.1 6.4 6.5 5.2 5.4 – 4.4 4.9 4.0 4.5 6.9 6.7 6.2 9.0 8.7 8.6 5.2 6.2 6.6
Std Deviation (1e-4) 0.6 0.9 1.0 1.7 1.1 1.0 1.3 – 1.2 1.1 0.6 1.2 1.2 1.4 1.5 1.8 1.9 2.3 1.1 1.2 1.4
Intensity Change (Log2) – – – 0.07 0.09 -0.23 -0.18 – -0.46 -0.33 -0.62 -0.44 0.18 0.13 0.03 0.55 0.51 0.5 -0.23 0.02 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus -1.2 -1.1 -2.3 -1.9 – -1.9 -2.7 -2.4 -2.5 -1.1 -1.4 -2.0 -2.4 -2.6 -2.0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0139 0.6641 0.5753 -0.2649 -0.0402 0.5269 1.369 2.1158 1.8289 1.4486 1.4732 1.5718 0.5178 1.0257 0.968 0.8694 0.7005 0.7315
Actin 0.0158 0 0.0115 0.0003 0.0075 0.0014 0.014 0.0001 0.004 0 0.0015 0.0022 0.0093 0 0.0002 0 0.0003 0
Bud 0.0002 0 0.0005 0.0001 0.0029 0.0001 0.0002 0.0005 0.0002 0 0.0003 0 0.0002 0 0.0001 0 0 0
Bud Neck 0.0008 0.0001 0.0016 0.0002 0.0013 0.0035 0.0014 0.0003 0.0006 0.0002 0.0011 0.0011 0.0022 0 0.0002 0 0.0049 0.0014
Bud Periphery 0.0003 0 0.0007 0.0004 0.0073 0.0002 0.0007 0.0013 0.0004 0.0002 0.0007 0.0001 0.0004 0 0.0001 0 0 0
Bud Site 0.0025 0 0.0126 0.0006 0.0027 0.0004 0.0011 0.0086 0.0005 0.0001 0.002 0.0001 0.0009 0 0.0001 0 0.0002 0.0001
Cell Periphery 0.0003 0 0.0002 0.0003 0.0006 0 0.0002 0.0003 0.0001 0.0001 0.0009 0 0.0002 0 0 0 0 0
Cytoplasm 0.0111 0.0001 0.0013 0.0009 0.0079 0.0021 0.0045 0.0005 0.0012 0 0.0042 0.001 0.001 0.0001 0.001 0.0001 0.0019 0.0009
Cytoplasmic Foci 0.0155 0 0.0139 0.003 0.0038 0.0046 0.0051 0.0001 0.0038 0 0.0009 0.0011 0.0004 0 0 0 0.0135 0.0008
Eisosomes 0.0004 0 0.0001 0 0.0002 0 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0021 0 0.0002 0.0025 0.0063 0.0003 0.0012 0.0002 0.0013 0 0.0056 0.0003 0.0014 0 0.0003 0 0.0003 0
Endosome 0.0211 0 0.0099 0.0196 0.0361 0.0024 0.0097 0.0004 0.0112 0 0.0074 0.0017 0.0033 0 0.0003 0 0.0019 0.0002
Golgi 0.0057 0 0.0066 0.0028 0.0079 0.0016 0.0035 0 0.002 0 0.0017 0.0007 0.0009 0 0 0 0.0002 0
Lipid Particles 0.0195 0 0.0183 0.0483 0.0246 0.0044 0.0166 0.0001 0.0081 0 0.0027 0.0014 0.0009 0 0 0 0.0025 0
Mitochondria 0.0099 0.0001 0.0142 0.0079 0.0606 0.0012 0.0111 0.0002 0.0031 0.0026 0.0022 0.0009 0.001 0.0001 0.0005 0.0001 0.0002 0.0001
None 0.0466 0 0.001 0.0007 0.0068 0.0003 0.0022 0.0004 0.0017 0 0.0005 0.0011 0.001 0 0.0002 0 0.0046 0.0001
Nuclear Periphery 0.017 0.0014 0.0087 0.011 0.055 0.0062 0.0077 0.0026 0.0062 0.0008 0.0132 0.0029 0.0071 0.0042 0.0068 0.0026 0.0061 0.0028
Nucleolus 0.017 0.0107 0.0105 0.0142 0.0431 0.0261 0.0194 0.013 0.0088 0.0294 0.0253 0.0225 0.0154 0.006 0.0087 0.0039 0.0316 0.0199
Nucleus 0.7575 0.9859 0.8724 0.8703 0.6881 0.9204 0.881 0.9692 0.9395 0.9653 0.9208 0.9508 0.9485 0.9768 0.9798 0.9929 0.9051 0.9524
Peroxisomes 0.0202 0 0.0108 0.0007 0.0031 0.0068 0.0046 0 0.0012 0 0.0002 0.0018 0.0002 0 0 0 0.0004 0
Punctate Nuclear 0.0324 0.0013 0.0023 0.0025 0.0221 0.0165 0.0118 0.0012 0.003 0.0004 0.0054 0.01 0.003 0.0125 0.0008 0.0002 0.0258 0.0208
Vacuole 0.0027 0 0.0019 0.009 0.0065 0.0009 0.0025 0.001 0.0021 0.0003 0.0026 0.0002 0.0018 0 0.0005 0 0.0003 0.0002
Vacuole Periphery 0.0012 0 0.0007 0.0048 0.0057 0.0004 0.0012 0.0001 0.0009 0.0006 0.0009 0.0002 0.0006 0 0.0002 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.6259 7.8658 3.8912 8.5903 11.7887 2.9314 9.9414 8.9464 10.4607 9.9486
Translational Efficiency 1.3052 1.0353 1.4241 0.7109 0.7015 2.6868 0.9228 0.9792 0.7017 0.7435

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2148 1419 1543 1129 1480 1771 125 267 3628 3190 1668 1396

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 610.97 735.86 1019.59 895.73 759.12 768.75 1017.10 929.46 671.41 754.12 1019.40 902.18
Standard Deviation 71.68 128.78 124.66 127.47 91.72 106.34 96.15 124.83 108.51 117.99 122.75 127.66
Intensity Change Log 2 — 0.268330 0.738816 0.551962 — 0.018187 0.422062 0.292065 — 0.135119 0.571956 0.413776

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000046 0.000639 0.000939 0.001260 0.000121 0.000242 0.001569 0.001311 0.000076 0.000419 0.000986 0.001270
Bud Neck 0.002522 0.005968 0.023584 0.021877 0.002385 0.006223 0.005726 0.015550 0.002466 0.006110 0.022246 0.020667
Bud Site 0.001990 0.002548 0.003000 0.008764 0.000419 0.002396 0.001234 0.006607 0.001349 0.002464 0.002867 0.008351
Cell Periphery 0.000321 0.000177 0.000076 0.000190 0.000112 0.000115 0.000091 0.000176 0.000236 0.000143 0.000077 0.000187
Cytoplasm 0.094509 0.030871 0.005352 0.027766 0.026475 0.020711 0.008659 0.033154 0.066755 0.025230 0.005599 0.028796
Cytoplasmic Foci 0.009304 0.003928 0.000102 0.005081 0.003309 0.003020 0.000248 0.008202 0.006858 0.003424 0.000113 0.005678
Eisosomes 0.000021 0.000027 0.000027 0.000031 0.000018 0.000021 0.000054 0.000039 0.000020 0.000024 0.000029 0.000033
Endoplasmic Reticulum 0.001301 0.005274 0.006670 0.007587 0.002070 0.003990 0.011810 0.007441 0.001615 0.004561 0.007056 0.007559
Endosome 0.000489 0.001049 0.000496 0.009524 0.000424 0.001907 0.000598 0.013206 0.000462 0.001525 0.000503 0.010228
Golgi 0.000092 0.000556 0.000122 0.006641 0.000280 0.000871 0.000131 0.011932 0.000169 0.000731 0.000123 0.007653
Lipid Particles 0.001621 0.001462 0.000245 0.000806 0.001863 0.001602 0.000654 0.014980 0.001720 0.001539 0.000276 0.003517
Mitochondria 0.001078 0.003868 0.002392 0.007445 0.001367 0.005891 0.001193 0.002202 0.001196 0.004991 0.002302 0.006442
Mitotic Spindle 0.000079 0.002160 0.004957 0.101618* 0.001669 0.006423 0.005105 0.133654* 0.000728 0.004527 0.004968 0.107745*
None 0.024380 0.019954 0.002328 0.021611 0.009533 0.009726 0.002247 0.025459 0.018324 0.014276 0.002322 0.022347
Nuclear Periphery 0.001213 0.001097 0.002022 0.004296 0.001277 0.001742 0.001529 0.002184 0.001239 0.001455 0.001985 0.003892
Nuclear Periphery Foci 0.000408 0.001704 0.000799 0.004497 0.000602 0.001406 0.001302 0.006249 0.000487 0.001538 0.000836 0.004832
Nucleolus 0.005826 0.020953 0.016746 0.017781 0.009783 0.015382 0.008426 0.019757 0.007440 0.017860 0.016122 0.018159
Nucleus 0.853092* 0.894581* 0.925649* 0.732877* 0.936802* 0.911862* 0.943777* 0.666656* 0.887241* 0.904175* 0.927008* 0.720212*
Peroxisomes 0.000326 0.001210 0.000346 0.002268 0.000523 0.000921 0.000464 0.000791 0.000406 0.001050 0.000355 0.001985
Vacuole 0.001183 0.001384 0.003715 0.013019 0.000791 0.004421 0.005009 0.026808 0.001023 0.003070 0.003812 0.015656
Vacuole Periphery 0.000199 0.000590 0.000434 0.005062 0.000176 0.001128 0.000173 0.003644 0.000189 0.000889 0.000415 0.004791

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -13.07 -21.29 -20.76 -12.79 -5.53 -6.03 -8.52 -11.71 -11.00 -0.68 -14.88 -22.32 -23.53 -18.66 -6.05
Bud Neck -5.45 -20.38 -16.66 -14.14 -2.37 -4.59 -4.41 -9.71 -8.16 -5.96 -6.93 -20.73 -18.92 -16.10 -2.90
Bud Site -0.84 -2.88 -8.00 -7.31 -6.43 -3.77 -4.57 -7.68 -5.96 -5.88 -2.34 -4.79 -10.81 -9.26 -7.66
Cell Periphery 1.80 3.03 2.17 1.33 -4.78 -0.28 -0.70 -3.50 -3.38 -2.39 1.96 3.16 1.69 -0.89 -5.73
Cytoplasm 14.03 24.64 17.35 3.00 -8.37 1.99 5.05 1.30 -0.18 -3.13 14.51 25.65 15.11 1.68 -9.46
Cytoplasmic Foci 4.48 9.94 7.33 2.83 -3.35 0.16 4.75 0.92 0.85 -2.09 4.44 11.09 6.83 2.96 -3.97
Eisosomes -3.65 -7.70 -13.33 -9.11 -7.43 -2.04 -6.48 -8.35 -7.92 -1.19 -3.77 -10.23 -15.95 -13.63 -8.20
Endoplasmic Reticulum -14.30 -18.34 -19.45 -9.32 -3.96 -6.46 -9.26 -9.05 -7.05 1.23 -14.17 -19.97 -21.23 -12.87 -3.95
Endosome -3.01 -1.95 -5.25 -4.07 -5.02 -3.69 -3.09 -2.66 -1.79 -2.32 -4.55 -3.20 -5.89 -3.92 -5.55
Golgi -3.91 -6.86 -2.62 -1.98 -2.49 -1.36 0.50 -1.31 -1.05 -1.36 -2.58 -0.13 -2.83 -2.06 -2.83
Lipid Particles 0.63 5.20 3.47 2.76 -4.93 0.50 1.84 -0.72 -0.83 -1.09 0.73 5.19 0.66 0.26 -1.82
Mitochondria -2.97 -9.06 -4.59 -0.79 -2.44 -3.70 -0.16 -2.82 2.67 -4.88 -4.86 -6.57 -4.75 0.55 -2.56
Mitotic Spindle -3.26 -5.75 -15.55 -15.11 -14.28 -2.76 -1.21 -8.94 -8.66 -7.96 -4.16 -5.21 -17.82 -17.11 -16.51
None 1.71 12.43 4.46 2.66 -6.78 -0.10 5.22 -1.68 -1.63 -4.34 2.61 13.71 2.50 0.33 -7.92
Nuclear Periphery 0.68 -5.86 -10.84 -11.33 -6.28 -1.92 -1.64 -6.82 -5.55 -3.71 -1.55 -6.11 -12.25 -11.31 -6.82
Nuclear Periphery Foci -4.55 -2.52 -7.13 -5.42 -5.55 -2.41 -1.82 -4.07 -3.74 -2.73 -4.70 -2.79 -8.12 -6.72 -6.40
Nucleolus -9.90 -11.76 -12.62 -7.29 -6.25 -3.88 -1.77 -5.74 -4.75 -3.73 -10.19 -10.75 -13.49 -9.42 -7.07
Nucleus -5.05 -7.78 14.51 17.59 19.82 4.31 1.79 14.30 12.99 11.65 -2.83 -3.13 21.54 22.86 23.05
Peroxisomes -4.38 -2.30 -2.94 0.28 -2.48 -1.83 -0.87 -2.46 -0.78 -1.69 -4.70 -0.85 -3.09 -0.30 -2.89
Vacuole -1.46 -6.10 -9.80 -9.59 -7.03 -3.94 -4.50 -5.89 -5.17 -4.47 -4.21 -6.82 -11.35 -9.81 -8.45
Vacuole Periphery -2.14 -2.06 -2.41 -1.65 -2.06 -4.04 -0.96 -1.70 -0.02 -1.61 -4.55 -2.25 -2.74 -1.22 -2.32
Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cdc23

Cdc23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available