Standard name
Human Ortholog
Description Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.97 0.95 0.87 0.89 0.86 0.85 0.75 0.82 0.75 0.99 0.99 0.99 0.97 0.97 0.99 0.97 0.95 0.91 0.88 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 0.11 0.21 0.13 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 2 0 1 4 1 4 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 4 0 0 2 0 0 0 2 1 1 0 0 0 0 1 0 0 1 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 4 5 4 4 8 13 12 10 10 7 5 5 6 3 16 25 16 0 0 0 0 0 0
Cytoplasm 723 240 303 121 258 208 278 261 161 213 145 173 141 135 135 485 324 270 692 228 293 59 91 97
Endoplasmic Reticulum 4 3 6 2 2 8 13 16 7 11 5 3 2 2 1 13 5 17 6 3 18 1 3 0
Endosome 0 0 0 0 0 0 1 1 0 2 0 0 0 0 0 1 0 0 1 0 0 0 0 0
Golgi 1 0 0 1 1 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 1 0 1 2 3 8 10 16 20 59 23 58 0 0 0 5 0 1 1 0 0 0 1 0
Nucleus 0 1 0 0 0 2 2 3 1 2 0 2 0 1 1 2 6 2 1 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 1 0 1 1 5 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 1 4 0 8 10 14 10 1 17 2 9 0 0 0 5 4 0 2 3 0 0 2 2
Unique Cell Count 730 243 313 125 271 238 314 303 189 285 177 232 143 137 137 500 334 273 712 239 323 68 102 107
Labelled Cell Count 733 249 323 130 277 250 331 321 206 322 189 253 148 144 140 529 365 306 712 239 323 68 102 107


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.6 24.6 21.1 20.0 16.5 14.2 12.9 12.0 11.4 10.3 10.8 10.5 19.7 18.9 18.3 25.9 32.2 31.4 19.2 19.5 20.0
Std Deviation (1e-4) 3.2 5.2 3.5 3.5 2.6 2.5 2.6 2.3 2.5 2.0 2.3 1.9 3.2 3.1 2.7 7.5 8.0 8.2 5.2 4.9 5.1
Intensity Change (Log2) – – – -0.08 -0.36 -0.57 -0.71 -0.81 -0.88 -1.04 -0.97 -1.0 -0.1 -0.16 -0.21 0.29 0.61 0.57 -0.14 -0.11 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.4 1.9 1.8 2.4 1.5 0 0 0 0 0 1.4 3.6 2.8
Cytoplasm 0 -1.0 -4.2 -4.0 -4.8 -4.8 -7.8 -5.7 -7.7 1.1 1.1 1.1 0.2 0.1 1.7
Endoplasmic Reticulum 0 0 1.1 1.6 2.2 0 1.4 0 0 0 0 0 0.6 -0.4 2.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.7 3.8 5.6 8.3 6.2 9.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.4 2.2 2.4 1.7 0 3.1 0 2.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.3887 19.386 17.1037 15.5235 16.5239 17.447 16.9093 23.1403 21.504 22.2696 19.5923 20.8168 17.6224 23.9029 21.5993 19.9351 20.0921 22.4025
Actin 0.0305 0.0004 0.0138 0.0005 0.0002 0.0007 0.0182 0.0003 0.0069 0.0001 0.0001 0.0024 0.0051 0.0036 0.0215 0.043 0.014 0.0003
Bud 0.0008 0.0002 0.0009 0.0005 0.0003 0.0003 0.0004 0 0.001 0.0001 0.0003 0.0006 0.0003 0.0005 0.0004 0.0004 0.0002 0.0001
Bud Neck 0.0044 0.0002 0.0006 0.0004 0.0007 0.0011 0.0009 0.0001 0.0004 0.0003 0.0004 0.0006 0.0006 0.0002 0.0005 0.0014 0.0004 0.0005
Bud Periphery 0.0007 0 0.001 0.0002 0.0001 0.0002 0.0003 0 0.0004 0 0.0001 0.0009 0.0003 0.0003 0.0006 0.0004 0.0002 0
Bud Site 0.0008 0.0005 0.006 0.0002 0.0001 0.0001 0.005 0.0001 0.0008 0 0.0004 0.0002 0.0061 0.0051 0.0041 0.0017 0.0001 0
Cell Periphery 0.0004 0.0002 0.0002 0.0002 0 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0.0002 0.0007 0.0003 0.0001 0 0
Cytoplasm 0.8155 0.9651 0.9032 0.9393 0.9641 0.8876 0.7824 0.9947 0.9404 0.9719 0.9161 0.9336 0.7958 0.9786 0.9174 0.8485 0.8939 0.9646
Cytoplasmic Foci 0.0097 0.0015 0.0084 0.0035 0.0029 0.003 0.0347 0.0008 0.0057 0.0018 0.0161 0.0038 0.0451 0.0017 0.0081 0.0064 0.0106 0.0023
Eisosomes 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0.0002 0 0.0002 0.0003 0.0001 0
Endoplasmic Reticulum 0.0406 0.0259 0.0122 0.011 0.0032 0.0551 0.0115 0.002 0.0044 0.0148 0.0053 0.0047 0.0106 0.0033 0.0174 0.003 0.0039 0.0101
Endosome 0.0331 0.0016 0.0118 0.0156 0.0106 0.0089 0.0565 0.0005 0.0157 0.0025 0.0338 0.0163 0.0156 0.0008 0.007 0.0265 0.0272 0.0038
Golgi 0.0111 0.0002 0.0085 0.001 0.0004 0.0015 0.0257 0.0001 0.0062 0.0001 0.0009 0.0021 0.0042 0.0003 0.0026 0.004 0.0027 0.0006
Lipid Particles 0.0096 0.0001 0.0031 0.0001 0 0.0002 0.0117 0 0.0016 0 0.0004 0.0004 0.0149 0.0006 0.0058 0.0016 0.004 0
Mitochondria 0.0035 0.0001 0.0043 0.0005 0.0003 0.0308 0.0067 0 0.0072 0.0001 0.0008 0.0206 0.002 0.0001 0.0033 0.0496 0.0013 0.0099
None 0.0027 0.0002 0.0013 0.0003 0.0002 0.0003 0.0103 0.0002 0.0011 0.0002 0.002 0.0004 0.0054 0.0008 0.0019 0.0006 0.0032 0.0003
Nuclear Periphery 0.0121 0.0015 0.0081 0.0048 0.0016 0.0026 0.0083 0.0004 0.0017 0.0034 0.0028 0.0033 0.0059 0.0009 0.0021 0.0021 0.0172 0.0044
Nucleolus 0.0004 0 0.0002 0 0 0 0.0011 0 0.0002 0 0.0001 0.0002 0.0008 0.0001 0.0002 0.0001 0.0005 0
Nucleus 0.0082 0.001 0.0051 0.0025 0.0019 0.0017 0.0069 0.0005 0.0016 0.0021 0.0022 0.0018 0.0073 0.0011 0.0013 0.0022 0.0026 0.0019
Peroxisomes 0.0016 0 0.0039 0 0 0.0001 0.0044 0 0.001 0 0.0002 0.0002 0.0407 0.0001 0.0013 0.0034 0.0016 0
Punctate Nuclear 0.0014 0 0.0009 0.0001 0 0.0001 0.0092 0 0.0006 0.0001 0.0004 0.0001 0.0362 0.0003 0.0015 0.0006 0.0127 0.0001
Vacuole 0.011 0.0011 0.0045 0.0163 0.0106 0.0044 0.0042 0.0002 0.0022 0.0022 0.0148 0.0052 0.002 0.0007 0.0017 0.0029 0.0021 0.0007
Vacuole Periphery 0.0018 0.0002 0.002 0.0031 0.0027 0.0012 0.0013 0 0.0009 0.0003 0.0026 0.0026 0.0005 0.0001 0.0009 0.001 0.0014 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 110.9074 98.8277 99.1895 70.1304 85.4508 63.7453 78.6265 61.8221 51.0012 72.8289
Translational Efficiency 0.9008 1.0668 0.8432 0.7913 0.8194 0.9469 0.8812 0.7828 0.8503 0.8587

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
872 1188 1335 1992 1885 829 131 2397 2757 2017 1466 4389

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1420.86 1558.97 2020.94 2230.35 1491.48 1551.79 2048.66 2467.85 1469.14 1556.02 2023.42 2360.06
Standard Deviation 214.92 322.71 295.66 360.63 230.90 277.14 346.79 431.21 228.34 304.82 300.68 417.80
Intensity Change Log 2 — 0.133829 0.508262 0.650506 — 0.057189 0.457936 0.726510 — 0.095088 0.482708 0.689929

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000199 0.000172 0.000262 0.000155 0.000139 0.000192 0.000349 0.000170 0.000158 0.000180 0.000270 0.000163
Bud Neck 0.000349 0.000431 0.002194 0.001568 0.000251 0.000580 0.005620 0.002482 0.000282 0.000492 0.002500 0.002067
Bud Site 0.013449 0.006231 0.006622 0.010237 0.006350 0.007956 0.014737 0.014606 0.008595 0.006940 0.007347 0.012623
Cell Periphery 0.000336 0.000274 0.000881 0.001190 0.000220 0.000357 0.000434 0.002062 0.000257 0.000308 0.000841 0.001667
Cytoplasm 0.829090* 0.927594* 0.864763* 0.824763* 0.902099* 0.876973* 0.458507* 0.835399* 0.879007* 0.906789* 0.828461* 0.830572*
Cytoplasmic Foci 0.089866 0.022481 0.014933 0.011424 0.044543 0.035130 0.027622 0.014134 0.058878 0.027680 0.016067 0.012904
Eisosomes 0.000002 0.000002 0.000002 0.000002 0.000002 0.000002 0.000007 0.000002 0.000002 0.000002 0.000002 0.000002
Endoplasmic Reticulum 0.009784 0.001846 0.001482 0.000156 0.005813 0.001812 0.002059 0.000157 0.007069 0.001832 0.001534 0.000156
Endosome 0.005422 0.002048 0.003548 0.001701 0.002730 0.004482 0.017420 0.001448 0.003581 0.003048 0.004787 0.001563
Golgi 0.014252 0.006966 0.011044 0.004412 0.007883 0.006381 0.006193 0.004553 0.009897 0.006726 0.010610 0.004489
Lipid Particles 0.001828 0.001678 0.005739 0.005526 0.001400 0.001193 0.009575 0.005856 0.001535 0.001479 0.006082 0.005706
Mitochondria 0.000104 0.000205 0.000068 0.000269 0.000151 0.000204 0.000034 0.000381 0.000136 0.000204 0.000065 0.000330
Mitotic Spindle 0.002426 0.001797 0.008930 0.006207 0.001486 0.005057 0.004457 0.005258 0.001783 0.003137 0.008531 0.005689
None 0.000310 0.001404 0.000659 0.000465 0.000501 0.000694 0.000351 0.000528 0.000441 0.001112 0.000631 0.000500
Nuclear Periphery 0.000283 0.000111 0.000283 0.000054 0.000058 0.000054 0.000425 0.000038 0.000129 0.000088 0.000296 0.000046
Nuclear Periphery Foci 0.003007 0.004079 0.010721 0.001852 0.001976 0.002655 0.010500 0.001110 0.002302 0.003494 0.010701 0.001447
Nucleolus 0.000024 0.000075 0.000044 0.000304 0.000048 0.000132 0.000198 0.000579 0.000040 0.000099 0.000058 0.000454
Nucleus 0.002181 0.006211 0.010505 0.008136 0.003188 0.007623 0.014396 0.005997 0.002870 0.006791 0.010853 0.006968
Peroxisomes 0.000114 0.000102 0.000046 0.000306 0.000083 0.000159 0.000265 0.000350 0.000092 0.000125 0.000066 0.000330
Vacuole 0.016284 0.012591 0.051904 0.115064 0.014471 0.041621 0.418363* 0.098241 0.015044 0.024523 0.084650 0.105877
Vacuole Periphery 0.010691 0.003704 0.005368 0.006208 0.006608 0.006741 0.008490 0.006645 0.007899 0.004952 0.005647 0.006447

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.92 -3.09 1.17 0.27 3.73 -1.13 -5.29 -1.66 -0.21 3.25 -0.98 -5.17 -1.19 -0.18 3.50
Bud Neck -1.25 -10.36 -5.65 -5.40 -0.02 -5.25 -8.06 -6.01 -5.24 3.83 -4.60 -12.66 -8.21 -7.47 0.22
Bud Site 4.99 4.29 1.03 -5.08 -4.23 -1.38 -3.27 -7.96 -5.38 -0.17 1.99 0.40 -6.32 -7.77 -6.61
Cell Periphery 1.72 -6.83 -5.69 -6.11 -1.14 -0.60 -4.08 -8.12 -5.26 -6.22 -0.54 -8.22 -9.96 -7.92 -4.42
Cytoplasm -11.33 -1.88 2.95 18.32 5.65 3.69 15.94 13.84 6.81 -12.77 -5.10 8.87 13.59 18.17 0.38
Cytoplasmic Foci 14.00 15.94 15.16 2.55 -0.10 3.00 3.78 9.06 5.13 2.35 12.85 19.04 17.05 4.97 -0.80
Eisosomes 1.56 -0.85 -0.78 -2.00 0.02 -1.14 -6.64 -2.68 -0.59 5.38 -0.14 -3.80 -2.79 -1.88 0.85
Endoplasmic Reticulum 5.09 5.62 6.61 3.06 5.34 4.13 4.02 6.58 3.46 2.31 6.27 7.14 9.25 4.39 5.73
Endosome 6.67 2.83 7.77 1.02 6.70 -2.95 -4.18 4.28 5.24 4.66 1.51 -3.01 8.00 4.91 8.67
Golgi 6.29 2.63 9.42 3.44 7.82 1.62 1.73 4.91 1.72 0.44 4.73 -0.84 9.88 3.60 7.97
Lipid Particles 0.37 -9.81 -5.77 -5.92 1.15 0.98 -5.37 -6.89 -7.14 2.81 0.12 -12.19 -9.14 -9.06 1.77
Mitochondria -0.87 1.09 -2.05 -0.31 -2.67 -0.64 5.19 -1.50 -1.01 -2.33 -0.89 3.28 -2.07 -0.96 -2.91
Mitotic Spindle 0.56 -4.56 -4.06 -4.81 0.23 -2.16 -1.85 -6.04 -2.33 0.42 -1.65 -5.85 -7.37 -5.35 0.35
None -3.30 -3.04 -2.95 2.73 1.84 -1.53 1.37 -1.41 0.68 -2.88 -3.26 -2.08 -2.00 2.74 1.35
Nuclear Periphery 0.67 -0.18 0.81 0.42 5.77 0.10 -3.57 -0.35 -1.19 3.57 0.45 -2.37 0.70 0.36 6.73
Nuclear Periphery Foci -1.29 -7.70 1.10 3.38 10.70 -1.65 -4.56 2.74 3.43 4.89 -2.81 -11.03 2.01 4.88 12.22
Nucleolus -1.68 -6.90 -2.69 -2.39 -2.55 -1.49 -5.20 -2.70 -2.32 -2.09 -1.92 -2.39 -3.68 -3.29 -3.54
Nucleus -7.56 -11.96 -13.97 -5.86 1.48 -7.43 -5.99 -7.89 -0.44 4.12 -9.72 -12.45 -14.37 -4.34 3.93
Peroxisomes 0.51 5.14 -5.32 -5.17 -6.70 -2.46 -4.85 -5.49 -3.84 -2.17 -1.82 2.13 -7.53 -6.18 -7.94
Vacuole 1.56 -11.48 -24.23 -25.39 -11.81 -7.83 -13.94 -24.37 -11.19 10.78 -5.50 -15.74 -34.75 -26.77 -3.69
Vacuole Periphery 4.43 3.63 3.51 -2.10 -0.43 0.03 0.48 0.58 0.38 -0.15 3.29 3.13 2.73 -1.22 -0.82
Standard name
Human Ortholog
Description Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Nmd3

Nmd3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nmd3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available