Standard name
Human Ortholog
Description Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0 0.08 0.06 0.08 – 0.09 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.05 0.05 0 0 0 0.06 0.08 0 0.12 – 0.09 0.08 0.05 0 0 0 0 0.06 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.05 0.08 0 0.05 0.05 – 0.07 0 0.08 0.09 0.15 0.09 0 0.06 0 0 0 0 0 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 – 0 0 0.08 0.18 0.17 0.1 0.19 0.11 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.06 0 0 0 0 0 0 0.07 – 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.28 0.21 0.07 0.12 0.09 0.11 0.12 0.08 0.16 – 0.13 0.09 0.22 0.17 0.15 0.18 0.15 0.44 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0.15 0.21 0.24 0.16 0.1 0.06 0 0 0 0 0 0
SpindlePole 0.84 0.85 0.95 0.89 0.9 0.86 0.88 0.89 0.83 – 0.8 0.91 0.81 0.8 0.7 0.74 0.85 0.89 0.89 0.86 0.9 0.87 0.84 0.78
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 0 2 4 3 0 – 0 5 0 0 0 0 0 0 0 0 0 0 1 2
Bud 6 8 12 7 19 12 33 38 29 – 26 24 1 1 1 0 0 0 3 3 2 8 13 20
Bud Neck 3 5 9 4 11 5 15 23 15 – 10 10 0 0 1 0 0 0 2 2 3 2 6 6
Bud Site 14 16 9 10 11 19 32 23 44 – 25 34 11 2 5 0 2 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 10 7 18 18 24 18 28 19 – 19 13 18 29 52 8 3 1 0 4 2 2 5 12
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 1 0 1 0
Endosome 11 17 1 3 5 10 3 5 2 – 2 4 20 59 60 9 15 2 8 19 13 4 6 4
Golgi 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 1 0 0 2 1 3 0 0 0
Mitochondria 15 21 3 3 3 8 9 15 26 – 22 15 4 0 2 1 2 0 1 5 0 0 3 3
Nucleus 7 3 6 4 4 5 11 16 9 – 7 8 0 0 1 1 0 0 2 0 1 0 0 0
Nuclear Periphery 0 1 0 0 1 0 0 0 1 – 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Nucleolus 73 70 26 31 36 33 49 48 59 – 37 36 53 55 51 17 12 8 5 4 6 0 1 1
Peroxisomes 8 12 2 0 6 10 6 9 3 – 7 4 36 70 84 15 8 1 1 5 4 3 5 6
SpindlePole 222 290 381 223 350 261 361 525 313 – 233 374 190 261 241 68 67 16 250 313 382 165 288 215
Vac/Vac Membrane 3 3 1 0 1 1 0 0 0 – 1 1 1 3 1 6 1 0 0 1 1 0 2 0
Unique Cell Count 263 340 399 250 391 303 411 593 377 292 413 236 328 345 92 79 18 282 366 427 190 343 277
Labelled Cell Count 366 456 459 305 465 390 541 733 520 389 528 334 481 500 126 111 30 282 366 427 190 343 277


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 5.4 6.1 5.8 6.1 5.2 5.4 5.5 4.5 – 4.5 4.9 5.8 6.0 6.1 11.5 10.6 9.7 5.3 5.3 5.6
Std Deviation (1e-4) 0.8 1.0 0.9 1.5 1.1 1.4 1.3 1.3 1.1 – 1.6 1.0 1.2 1.5 1.6 2.8 1.9 1.9 1.3 1.4 1.3
Intensity Change (Log2) – – – -0.06 0.01 -0.24 -0.16 -0.13 -0.43 – -0.42 -0.3 -0.07 -0.02 -0.0 0.92 0.81 0.68 -0.19 -0.19 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud -0.2 1.3 0.7 3.1 2.4 0 – 0 1.9 0 -2.7 -2.8 0 0 0
Bud Neck -0.6 0.5 -0.6 1.2 1.4 0 – 0 0.2 0 0 0 0 0 0
Bud Site 1.3 0.5 2.7 3.6 1.4 0 – 0 3.8 1.7 0 -0.8 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 3.5 2.3 3.9 2.2 2.5 2.5 – 3.2 1.3 3.7 4.4 6.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 5.6 8.6 8.5 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 1.7 2.1 0 – 0 2.8 0 0 0 0 0 0
Nucleus 0 0 0 1.2 1.3 0.9 – 0.9 0.5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 2.6 1.4 2.1 2.7 0.9 0 – 0 1.2 5.9 4.4 3.7 3.7 2.6 0
Peroxisomes 0 0 2.8 0 0 0 – 0 0 7.6 9.4 10.1 0 0 0
SpindlePole -3.1 -3.2 -4.4 -3.9 -3.8 0 – 0 -2.8 -6.1 -6.6 -9.4 -6.7 -3.6 -1.3
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5234 3.4743 3.308 2.9255 3.5545 3.1969 1.6323 1.7032 1.9934 1.8669 1.7714 1.4329 1.3883 1.6639 1.6698 2.0143 1.4226 1.2356
Actin 0.0067 0.0001 0.0066 0 0.0054 0.0019 0.0242 0.0016 0.0067 0.0001 0.0338 0.0006 0.0087 0 0.0022 0.0001 0.0041 0
Bud 0.0002 0.0001 0.0002 0 0.0019 0 0.0004 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0 0.0006 0
Bud Neck 0.0037 0.0005 0.0004 0.0005 0.0003 0.0002 0.0009 0.0006 0.0009 0.0001 0.0005 0.0001 0.0014 0.0003 0.0001 0.0003 0.0007 0.0002
Bud Periphery 0.0004 0.0001 0.0005 0 0.0058 0.0001 0.0007 0 0.0001 0 0.0002 0 0.0001 0 0 0 0.0013 0
Bud Site 0.0084 0.0014 0.0007 0 0.0018 0.0001 0.0023 0.0024 0.0018 0 0.0004 0 0.0015 0.0004 0.0001 0.0001 0.0025 0
Cell Periphery 0.002 0.0001 0.0001 0 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0
Cytoplasm 0.0033 0.0063 0.0003 0 0.0003 0 0.0028 0.0001 0.0007 0.0001 0.0004 0 0.0015 0.0001 0.0006 0 0.0005 0.0003
Cytoplasmic Foci 0.0849 0.0249 0.0503 0.0514 0.1304 0.0095 0.0819 0.0264 0.0717 0.1207 0.1502 0.0243 0.0548 0.0426 0.0233 0.0484 0.0488 0.0112
Eisosomes 0.0004 0 0.0001 0 0.0002 0 0.0003 0 0.0001 0 0.0002 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0014 0.0004 0.0003 0 0.0002 0 0.0031 0 0.0013 0 0.0005 0.0003 0.0008 0 0.0002 0 0.0006 0.0001
Endosome 0.0225 0.0013 0.0037 0.0004 0.007 0.001 0.028 0.0007 0.0045 0.0006 0.0256 0.001 0.0071 0.0017 0.0016 0.0002 0.0112 0.0004
Golgi 0.0078 0.0003 0.0036 0.0006 0.0058 0.0017 0.0096 0.0015 0.0052 0.0014 0.0394 0.0012 0.0029 0.0011 0.0007 0.0005 0.0041 0.0003
Lipid Particles 0.0497 0.0029 0.0309 0.0057 0.0765 0.0053 0.034 0.0082 0.0456 0.0259 0.1398 0.0142 0.0297 0.0286 0.007 0.0034 0.0472 0.005
Mitochondria 0.0037 0.0001 0.003 0 0.0717 0.008 0.0093 0.0006 0.0033 0 0.0054 0.0006 0.0005 0.0001 0.0001 0 0.0129 0
None 0.0049 0.0132 0.0003 0 0.0009 0 0.0034 0.0001 0.0002 0 0.0003 0.0001 0.0025 0.0001 0.0017 0 0.0016 0.0006
Nuclear Periphery 0.0061 0.0004 0.0008 0.0002 0.0004 0.0006 0.0119 0.0002 0.0019 0.0004 0.0022 0.0019 0.0066 0.0007 0.0006 0.0003 0.0043 0.0004
Nucleolus 0.0119 0.0403 0.0024 0.0018 0.001 0.0051 0.0029 0.0242 0.0069 0.0007 0.0022 0.0017 0.0074 0.0175 0.003 0.0006 0.0089 0.0031
Nucleus 0.0045 0.0065 0.0004 0.0002 0.0004 0.0005 0.0021 0.0032 0.0006 0.0001 0.0003 0.0003 0.009 0.0076 0.0009 0.0002 0.003 0.0005
Peroxisomes 0.029 0.0007 0.0097 0.002 0.0643 0.007 0.0109 0.0046 0.0165 0.0101 0.0323 0.0041 0.0084 0.0066 0.0017 0.0025 0.0272 0.0007
Punctate Nuclear 0.7415 0.9001 0.8854 0.9369 0.6228 0.9586 0.7667 0.9256 0.8313 0.8397 0.5647 0.9492 0.8564 0.892 0.956 0.9433 0.8161 0.9771
Vacuole 0.0061 0.0005 0.0002 0 0.0018 0.0001 0.0028 0 0.0002 0 0.0006 0 0.0003 0.0006 0 0 0.0023 0
Vacuole Periphery 0.0009 0 0.0001 0 0.0009 0.0002 0.0019 0 0.0002 0 0.0008 0.0001 0.0003 0 0 0 0.0018 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.3267 15.9608 5.3296 7.7797 15.6256 13.3834 13.7172 8.9859 8.244 18.6049
Translational Efficiency 0.66 0.5879 0.7271 0.5503 0.6531 0.9583 0.6774 0.4422 0.4834 0.5014

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1801 281 1416 1929 2100 1530 1635 257 3901 1811 3051 2186

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 717.35 892.96 1051.03 986.55 785.14 792.83 913.91 1012.61 753.84 808.37 977.55 989.61
Standard Deviation 84.90 116.16 142.56 131.75 98.20 99.58 116.20 124.60 98.29 108.56 146.10 131.19
Intensity Change Log 2 — 0.315918 0.551055 0.459715 — 0.014062 0.219102 0.367057 — 0.166069 0.387130 0.412039

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000228 0.001490 0.000097 0.000217 0.000353 0.000548 0.000196 0.001503 0.000295 0.000694 0.000150 0.000368
Bud Neck 0.005530 0.003436 0.001460 0.001955 0.003924 0.002663 0.004231 0.002862 0.004665 0.002783 0.002945 0.002061
Bud Site 0.006196 0.010010 0.005372 0.013401 0.007549 0.007156 0.007246 0.023427 0.006924 0.007599 0.006377 0.014580
Cell Periphery 0.000188 0.000165 0.000019 0.000038 0.000180 0.000153 0.000104 0.000041 0.000184 0.000155 0.000065 0.000038
Cytoplasm 0.013874 0.017785 0.001755 0.006995 0.012662 0.008452 0.010828 0.002480 0.013222 0.009900 0.006617 0.006464
Cytoplasmic Foci 0.185100* 0.123628 0.006637 0.031288 0.101070 0.151233 0.024058 0.039917 0.139865 0.146950 0.015973 0.032302
Eisosomes 0.000246 0.000096 0.000010 0.000010 0.000168 0.000160 0.000036 0.000015 0.000204 0.000150 0.000024 0.000010
Endoplasmic Reticulum 0.000838 0.000871 0.000136 0.000187 0.001063 0.000908 0.000520 0.000256 0.000959 0.000902 0.000342 0.000195
Endosome 0.009423 0.015542 0.001248 0.002383 0.012852 0.016561 0.003027 0.005132 0.011269 0.016403 0.002201 0.002706
Golgi 0.015576 0.009054 0.000338 0.002656 0.012046 0.016799 0.001389 0.002183 0.013676 0.015597 0.000901 0.002600
Lipid Particles 0.028091 0.022648 0.000648 0.001472 0.023333 0.046490 0.001567 0.002073 0.025529 0.042791 0.001141 0.001543
Mitochondria 0.000864 0.000695 0.000068 0.001227 0.000557 0.002727 0.000381 0.000347 0.000698 0.002412 0.000236 0.001123
Mitotic Spindle 0.506477* 0.650354* 0.969993* 0.918732* 0.607171* 0.553618* 0.875072* 0.896920* 0.560683* 0.568628* 0.919126* 0.916167*
None 0.003968 0.000620 0.000081 0.000173 0.002564 0.002276 0.001720 0.000040 0.003212 0.002019 0.000959 0.000158
Nuclear Periphery 0.000369 0.000466 0.000149 0.000414 0.000507 0.000315 0.000467 0.000206 0.000443 0.000338 0.000320 0.000389
Nuclear Periphery Foci 0.100432 0.073537 0.002425 0.001567 0.118964 0.079706 0.015052 0.001663 0.110408 0.078748 0.009192 0.001579
Nucleolus 0.018750 0.020126 0.002231 0.000588 0.028493 0.007140 0.024387 0.000383 0.023995 0.009155 0.014104 0.000563
Nucleus 0.003176 0.005001 0.002109 0.000943 0.008210 0.001380 0.017601 0.000635 0.005886 0.001942 0.010411 0.000907
Peroxisomes 0.099651 0.041409 0.003374 0.008895 0.056858 0.100264* 0.009445 0.011916 0.076615 0.091132 0.006628 0.009250
Vacuole 0.000944 0.002728 0.001768 0.004012 0.001318 0.001318 0.002439 0.007513 0.001145 0.001537 0.002128 0.004424
Vacuole Periphery 0.000080 0.000339 0.000080 0.002848 0.000159 0.000134 0.000233 0.000488 0.000122 0.000166 0.000162 0.002570

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.38 -1.94 -2.43 2.14 -0.18 -2.26 -1.56 -1.33 -1.11 -1.23 -3.62 -2.39 -1.84 1.06 -0.95
Bud Neck -0.79 -2.31 -0.13 0.77 2.47 2.26 -5.31 -1.90 -2.59 1.15 2.13 -5.50 -1.15 -3.10 4.18
Bud Site -2.85 -3.29 -9.29 -4.83 -4.66 -0.83 -5.86 -4.23 -4.14 -3.01 -2.20 -6.44 -10.11 -9.11 -5.46
Cell Periphery 0.81 10.94 10.77 4.09 -0.43 -0.04 1.91 5.66 5.93 3.44 0.94 7.45 12.82 10.42 4.03
Cytoplasm -1.44 3.19 -0.72 1.13 -3.49 0.66 -2.20 1.32 0.82 2.99 -0.05 0.06 -0.74 -0.62 -0.75
Cytoplasmic Foci 4.91 25.73 19.15 5.51 -9.42 -5.36 14.48 8.11 11.57 -0.54 -0.30 28.41 20.59 16.69 -5.99
Eisosomes 11.09 24.70 25.89 7.98 3.67 1.57 19.27 23.47 19.08 8.33 6.67 31.12 35.80 21.65 11.72
Endoplasmic Reticulum -0.36 5.96 12.85 5.80 4.11 1.58 4.99 2.68 2.12 0.80 0.68 7.70 15.43 11.88 6.38
Endosome -3.27 7.20 8.26 6.58 0.65 -1.79 8.42 3.34 4.34 -0.64 -3.58 11.07 11.52 11.29 1.65
Golgi 6.50 22.32 17.70 5.58 -2.18 -2.91 20.32 16.39 17.36 1.42 0.42 30.12 22.01 17.98 -0.52
Lipid Particles 0.90 13.79 13.42 5.41 -1.90 -6.04 13.97 13.00 13.29 -0.46 -5.11 19.57 19.24 14.56 -0.40
Mitochondria -0.18 1.94 -1.07 -0.97 -2.11 -1.31 -0.91 0.24 1.34 0.89 -1.22 1.26 -1.38 0.43 -1.78
Mitotic Spindle -5.62 -38.94* -36.67* -11.08 3.86 2.28 -22.59* -15.78 -16.78 -2.26 -2.05 -42.07 -43.18 -31.82* -2.47
None 4.18 6.95 6.83 2.33 -0.66 0.72 1.08 5.83 4.71 4.48 2.36 5.09 8.75 4.76 4.02
Nuclear Periphery -1.71 -0.07 -0.15 0.75 -0.11 6.61 -1.08 0.33 -0.84 0.83 4.23 -0.98 0.47 -0.44 0.88
Nuclear Periphery Foci 3.05 22.37* 24.75* 8.15 5.07 5.94 22.20* 28.14* 20.48* 8.24 6.24 31.39 38.17 22.91* 10.71
Nucleolus -0.55 6.01 11.29 4.13 6.30 8.27 -1.06 7.55 2.10 7.66 7.12 2.13 16.86 7.70 12.58
Nucleus -2.16 -4.08 -1.24 1.20 3.03 4.96 -6.80 0.45 -1.72 5.83 3.73 -7.90 0.26 -1.93 7.23
Peroxisomes 6.22 22.96 21.62 5.94 -3.86 -7.65 15.56 12.91 17.20 1.29 -3.12 27.36 26.79 20.48 0.15
Vacuole -3.99 -4.10 -4.71 -3.07 0.35 -1.34 -3.43 -2.75 -2.72 -2.39 -4.15 -5.20 -5.35 -5.01 -1.88
Vacuole Periphery -2.72 -3.22 -2.89 -2.56 -2.75 0.56 -4.78 -0.98 -1.03 -0.39 -1.62 -5.73 -2.91 -2.85 -2.70
Standard name
Human Ortholog
Description Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
Localization
Cell Percentages spindle pole (96%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Spc97

Spc97


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spc97-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available