Standard name
Human Ortholog
Description Transcription factor; involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.06 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.18 0.21 0.23 0.26 0.22 0.22 – 0.12 0.16 0.14 0.14 0.1 0.19 0.24 0.16 0.16 0.19 0 0.1 0.1 0.05 0.06 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0 0 0 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 0.45 0.12 0.21 0.05 0.39 0.47 – 0.74 0.61 0.72 0.57 0.32 0.12 0.15 0.07 0.05 0.09 0 0.06 0.06 0 0.1 0.09
Nucleus 0.5 0.39 0.65 0.6 0.57 0.48 0.42 – 0.41 0.36 0.38 0.44 0.63 0.54 0.45 0.69 0.66 0.74 0.47 0.4 0.41 0.34 0.23 0.17
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.05 0.06 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.14 0.07 0 0.05 – 0 0.05 0 0.09 0 0 0 0 0 0 0.11 0.08 0.06 0.09 0.09 0.11
Vac/Vac Membrane 0.12 0.15 0 0 0.06 0.07 0 – 0 0.05 0 0 0.07 0.15 0.18 0.13 0.17 0.09 0.16 0.2 0.22 0.3 0.37 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2
Bud 0 0 0 0 1 3 3 – 10 12 6 9 0 0 0 0 1 0 0 3 0 8 4 13
Bud Neck 0 1 10 1 10 5 15 – 6 6 5 7 0 0 0 0 0 0 0 1 2 1 4 8
Bud Site 0 0 0 0 2 2 7 – 16 9 25 14 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 7 34 33 26 77 58 106 – 52 76 56 43 22 50 76 23 13 19 4 22 24 9 18 38
Endoplasmic Reticulum 0 2 2 0 1 1 1 – 1 0 0 0 0 0 2 4 2 6 0 0 0 0 2 4
Endosome 4 1 0 1 5 5 1 – 2 2 1 1 1 14 15 1 2 0 7 9 6 2 7 14
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Mitochondria 48 82 18 24 16 104 222 – 323 285 282 178 69 32 48 10 4 9 3 14 14 5 30 33
Nucleus 48 72 100 67 170 126 200 – 178 168 148 138 137 141 142 98 55 73 58 94 101 60 68 62
Nuclear Periphery 0 0 0 0 0 0 0 – 2 3 2 0 0 1 0 0 0 0 0 2 0 0 0 1
Nucleolus 3 1 0 0 0 1 2 – 4 0 5 0 2 2 1 1 1 2 11 11 13 10 10 10
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 1 1 9 16 22 5 23 – 19 22 11 27 0 5 3 5 1 3 13 19 15 16 27 40
Vac/Vac Membrane 12 28 6 2 19 18 9 – 9 24 9 6 16 39 57 18 14 9 19 47 54 54 108 111
Unique Cell Count 96 184 155 112 297 265 476 435 464 390 311 218 262 317 142 83 99 124 233 246 178 297 361
Labelled Cell Count 123 223 178 137 323 328 589 623 607 550 423 247 285 344 162 93 121 124 233 246 178 297 361


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.7 4.8 4.4 5.0 3.8 4.0 – 3.4 3.4 3.3 3.7 4.1 4.3 4.1 6.4 6.7 6.2 4.0 4.0 4.2
Std Deviation (1e-4) 0.5 0.6 0.8 1.2 1.1 1.1 1.2 – 1.0 0.8 1.2 0.8 0.8 1.0 0.8 1.2 1.5 1.2 1.1 1.2 1.1
Intensity Change (Log2) – – – -0.11 0.05 -0.32 -0.28 – -0.49 -0.48 -0.52 -0.36 -0.22 -0.16 -0.21 0.43 0.48 0.37 -0.25 -0.26 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.8 3.4 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0571 -0.6727 -1.0548 -0.0431 -0.0408 -0.5775 -0.1691 -0.5592 -0.3392 -0.2475 0.1256 -0.1484 -0.12 0.1295 -0.0793 -0.0857 -0.5995 -0.197
Actin 0.0625 0.0003 0.0002 0.0679 0.0321 0.0005 0.0573 0.0046 0.0078 0.0085 0.0502 0.0061 0.0037 0.0002 0.0051 0.0059 0.0006 0.0004
Bud 0.0007 0.0004 0.0003 0.001 0.0006 0.0001 0.0005 0.0004 0.0006 0.0006 0.0011 0.0007 0.0002 0.0002 0.0002 0.0003 0.0001 0.0002
Bud Neck 0.0057 0.0006 0.0007 0.0018 0.0017 0.0044 0.0014 0.0009 0.0024 0.0005 0.0011 0.0088 0.0007 0.0007 0.0007 0.0007 0.002 0.0046
Bud Periphery 0.0012 0.0005 0.0001 0.0013 0.0013 0.0001 0.0008 0.0004 0.0005 0.0003 0.0018 0.0009 0.0002 0.0002 0.0002 0.0009 0.0002 0.0004
Bud Site 0.0046 0.0016 0.0004 0.0071 0.0009 0.0005 0.0028 0.0021 0.0151 0.001 0.002 0.0008 0.0014 0.0011 0.0014 0.0003 0.0004 0.0004
Cell Periphery 0.0004 0.0001 0 0.0003 0.0002 0 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0002 0.0001 0 0.0002 0 0
Cytoplasm 0.0263 0.062 0.0255 0.016 0.0082 0.0376 0.0297 0.0801 0.0385 0.0408 0.0109 0.0386 0.0243 0.0536 0.0294 0.0219 0.0096 0.0141
Cytoplasmic Foci 0.0203 0.0105 0.0008 0.0139 0.0101 0.0044 0.0212 0.0044 0.0244 0.0507 0.0077 0.003 0.0091 0.0022 0.0118 0.0014 0.005 0.0007
Eisosomes 0.0007 0.0001 0 0.0006 0.0005 0 0.0003 0 0.0001 0.0001 0.0003 0 0.0001 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0076 0.0046 0.0012 0.0041 0.0072 0.0032 0.0043 0.0031 0.0024 0.0018 0.0041 0.0021 0.0086 0.0025 0.0014 0.0069 0.0008 0.0004
Endosome 0.0302 0.0065 0.0015 0.0258 0.0511 0.0052 0.0202 0.0042 0.0084 0.0233 0.1321 0.0076 0.0211 0.002 0.0137 0.016 0.0048 0.0021
Golgi 0.0083 0.0002 0 0.0174 0.0123 0.0001 0.0108 0.0007 0.0126 0.017 0.0344 0.0016 0.006 0.0001 0.0062 0.0046 0.0032 0.001
Lipid Particles 0.0245 0.0029 0.0003 0.0197 0.0282 0.0006 0.0179 0.0006 0.0071 0.0144 0.0546 0.0006 0.0116 0.0002 0.0021 0.0007 0.0081 0.0002
Mitochondria 0.0083 0.0016 0.0007 0.0126 0.0107 0.0003 0.0068 0.0009 0.0035 0.0024 0.0234 0.0226 0.004 0.0011 0.0015 0.0019 0.0191 0.0087
None 0.0424 0.1148 0.0615 0.0733 0.011 0.1134 0.0517 0.088 0.0404 0.0065 0.0632 0.0079 0.0313 0.0562 0.035 0.0086 0.0275 0.0052
Nuclear Periphery 0.0687 0.0336 0.029 0.0432 0.1103 0.0127 0.0172 0.0204 0.0373 0.0035 0.0203 0.0172 0.0109 0.0179 0.0106 0.0095 0.017 0.0061
Nucleolus 0.016 0.0136 0.0167 0.0078 0.0163 0.0338 0.008 0.0157 0.0114 0.0281 0.0133 0.02 0.017 0.0088 0.0178 0.005 0.0342 0.0256
Nucleus 0.5472 0.6484 0.8196 0.5427 0.3751 0.7162 0.6765 0.7291 0.6932 0.7438 0.4969 0.8298 0.8013 0.8203 0.8285 0.8282 0.8071 0.9119
Peroxisomes 0.0086 0.0003 0.0001 0.0058 0.0089 0.0001 0.0195 0.0028 0.0088 0.013 0.0017 0.0004 0.0023 0 0.0077 0 0.0067 0.0001
Punctate Nuclear 0.1103 0.0902 0.0396 0.1342 0.3001 0.0647 0.0492 0.0394 0.0824 0.0426 0.0494 0.0279 0.0422 0.0295 0.0254 0.0821 0.0526 0.0168
Vacuole 0.0028 0.0057 0.001 0.0019 0.0045 0.0017 0.0024 0.0016 0.0023 0.0008 0.0196 0.0026 0.003 0.0023 0.001 0.0031 0.0006 0.0009
Vacuole Periphery 0.0026 0.0013 0.0007 0.0016 0.0086 0.0003 0.0013 0.0004 0.0007 0.0004 0.0118 0.0007 0.0008 0.0007 0.0003 0.0016 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.9436 4.8612 3.9351 6.4178 8.0119 3.4586 10.1654 8.595 6.533 8.8046
Translational Efficiency 0.5263 0.7645 0.7002 0.4691 0.5033 0.9756 0.376 0.4846 0.6297 0.4451

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2013 1182 259 1328 1225 1542 1546 525 3238 2724 1805 1853

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 671.41 750.21 952.28 861.88 716.12 731.50 829.21 856.74 688.32 739.62 846.87 860.42
Standard Deviation 72.04 97.31 101.55 106.94 82.64 96.20 99.17 119.52 79.25 97.12 108.47 110.67
Intensity Change Log 2 — 0.160100 0.504192 0.360293 — 0.030657 0.211536 0.258656 — 0.094744 0.360565 0.308732

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000044 0.000453 0.001911 0.001867 0.000163 0.000313 0.000540 0.000996 0.000089 0.000374 0.000737 0.001620
Bud Neck 0.003666 0.006978 0.014355 0.024681 0.001894 0.007121 0.011796 0.006981 0.002995 0.007059 0.012163 0.019666
Bud Site 0.001124 0.002861 0.003023 0.018726 0.001129 0.003102 0.003369 0.015957 0.001126 0.002997 0.003319 0.017941
Cell Periphery 0.000103 0.000117 0.000109 0.000174 0.000184 0.000135 0.000117 0.000189 0.000133 0.000127 0.000116 0.000178
Cytoplasm 0.287177* 0.134521* 0.002634 0.053673 0.135059* 0.141274* 0.035940 0.106639* 0.229628* 0.138344* 0.031161 0.068680
Cytoplasmic Foci 0.051939 0.037144 0.000199 0.004241 0.050576 0.053994 0.000846 0.004476 0.051423 0.046682 0.000753 0.004308
Eisosomes 0.000045 0.000062 0.000074 0.000035 0.000081 0.000066 0.000031 0.000046 0.000059 0.000064 0.000037 0.000038
Endoplasmic Reticulum 0.000700 0.003142 0.014225 0.009406 0.001515 0.002473 0.004592 0.007195 0.001008 0.002763 0.005974 0.008780
Endosome 0.001706 0.004283 0.000643 0.007826 0.002770 0.004748 0.000891 0.003714 0.002109 0.004546 0.000855 0.006661
Golgi 0.000205 0.001483 0.000138 0.007276 0.002107 0.003707 0.000390 0.000890 0.000925 0.002742 0.000354 0.005467
Lipid Particles 0.007335 0.007010 0.000774 0.000724 0.016415 0.011392 0.000458 0.001216 0.010770 0.009491 0.000503 0.000864
Mitochondria 0.002599 0.007128 0.001615 0.003697 0.006289 0.010171 0.001593 0.001732 0.003995 0.008851 0.001596 0.003141
Mitotic Spindle 0.001016 0.008345 0.000694 0.025926 0.002352 0.010904 0.007579 0.021421 0.001522 0.009794 0.006591 0.024650
None 0.010947 0.011490 0.001936 0.006930 0.012623 0.010945 0.010568 0.044867 0.011581 0.011181 0.009329 0.017679
Nuclear Periphery 0.002067 0.003424 0.001031 0.003785 0.003747 0.004643 0.001560 0.001797 0.002703 0.004114 0.001484 0.003221
Nuclear Periphery Foci 0.000936 0.002864 0.000611 0.002233 0.001773 0.002278 0.000435 0.001064 0.001253 0.002532 0.000460 0.001902
Nucleolus 0.007961 0.016873 0.010358 0.003259 0.016513 0.019175 0.006470 0.004998 0.011196 0.018176 0.007028 0.003752
Nucleus 0.611694* 0.737775* 0.937423* 0.800079* 0.735671* 0.696478* 0.906586* 0.753569* 0.658597* 0.714398* 0.911011* 0.786901*
Peroxisomes 0.001883 0.004717 0.000590 0.001102 0.004535 0.008358 0.000235 0.001251 0.002886 0.006778 0.000286 0.001144
Vacuole 0.006580 0.008543 0.007261 0.019657 0.004075 0.007737 0.005415 0.020363 0.005632 0.008087 0.005680 0.019857
Vacuole Periphery 0.000275 0.000787 0.000398 0.004703 0.000529 0.000986 0.000589 0.000636 0.000371 0.000900 0.000561 0.003550

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.90 -12.10 -5.27 -3.14 3.09 -3.92 -12.25 -9.70 -8.42 -5.22 -7.94 -19.72 -7.18 -5.23 -2.13
Bud Neck -3.94 -5.06 -13.19 -10.78 -2.23 -6.98 -12.26 -9.09 -4.88 0.02 -7.06 -12.24 -15.60 -11.72 -4.44
Bud Site -3.15 -3.45 -6.40 -5.18 -3.58 -2.49 -3.81 -3.89 -3.09 -2.91 -3.85 -5.51 -7.48 -5.94 -5.33
Cell Periphery -2.68 -2.13 -4.78 -2.16 -0.92 2.48 4.15 0.91 -1.99 -4.29 0.19 2.17 -1.89 -2.28 -4.39
Cytoplasm 20.10 51.97 40.34 15.47 -14.77 -0.55 17.92 8.31 9.19 -5.30 17.08 43.65 36.79 20.02 -6.63
Cytoplasmic Foci 4.27 24.34 23.34 14.31 -3.67 -0.73 17.97 17.26 18.44 -3.58 1.61 30.04 29.00 23.29 -3.70
Eisosomes -6.16 -5.46 -2.20 4.46 4.40 2.79 11.07 1.91 -0.42 -7.33 -3.39 6.65 1.11 4.32 -5.30
Endoplasmic Reticulum -11.75 -15.41 -25.48 -18.55 1.59 -3.74 -17.76 -12.59 -11.19 -7.32 -10.48 -24.52 -27.75 -22.10 -11.32
Endosome -3.39 4.64 -2.22 1.40 -3.81 -2.23 4.03 0.14 2.20 -3.23 -4.44 5.57 -1.67 2.36 -4.64
Golgi -2.16 0.67 -2.22 -0.63 -2.25 -1.44 2.99 2.61 3.75 -0.69 -3.03 2.59 -1.07 1.27 -2.20
Lipid Particles -0.10 9.17 9.10 11.42 -0.62 3.24 12.33 11.51 11.30 -3.99 1.23 15.41 14.67 15.59 -4.88
Mitochondria -4.20 1.55 -1.09 3.94 -3.90 -2.57 3.54 3.27 8.78 -1.34 -5.66 3.95 2.07 8.79 -4.67
Mitotic Spindle -4.54 -1.85 -8.12 -4.69 -6.73 -4.62 -4.70 -5.20 -2.95 -3.00 -6.74 -5.62 -9.56 -5.46 -5.45
None -0.91 12.18 5.86 5.62 -7.23 1.18 3.66 -1.59 -2.03 -2.95 0.12 5.10 1.39 1.33 -2.29
Nuclear Periphery -2.57 1.74 -11.56 -5.17 -12.68 -1.15 2.41 -1.08 0.39 -5.27 -3.08 1.44 -9.69 -4.10 -11.92
Nuclear Periphery Foci -4.62 0.35 -3.26 2.39 -3.84 -1.40 5.54 0.01 1.20 -3.78 -4.74 4.04 -2.46 2.59 -6.05
Nucleolus -6.04 -0.65 5.96 9.59 3.92 -2.62 7.15 8.85 10.58 2.97 -6.93 5.22 10.62 14.49 5.50
Nucleus -10.91 -35.04 -19.80 -6.79 13.17 3.88 -18.96 -3.74 -6.85 9.56 -6.06 -38.72 -17.54 -11.18 15.22
Peroxisomes -3.69 3.74 4.08 6.28 1.20 -3.79 7.32 6.37 8.83 -2.09 -6.06 8.41 7.46 10.92 -1.65
Vacuole -3.31 -2.08 -11.73 -10.14 -9.80 -4.42 -5.02 -8.84 -7.69 -7.80 -4.69 -3.63 -14.65 -12.91 -13.24
Vacuole Periphery -5.02 -0.86 -2.28 -1.28 -2.13 -1.92 0.76 0.68 2.46 -0.22 -4.16 -0.07 -2.12 -0.76 -1.99
Standard name
Human Ortholog
Description Transcription factor; involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro
Localization
Cell Percentages nucleus (75%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Stb5

Stb5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stb5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available