Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.09 0.09 0.09 0.09 0.07 0.11
Bud 0 0 0 0.08 0 0 0.07 0.05 0.05 0 0.09 0.12 0 0 0.06 0 0 0 0.07 0 0.07 0.26 0.26 0.24
Bud Neck 0 0 0.1 0.06 0.07 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.1 0.14 0.1 0.05
Bud Site 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.84 0.86 0.81 0.67 0.82 0.7 0.7 0.86 0.64 0.52 0.48 0.51 0.88 0.86 0.84 0.82 0.85 0.83 0.52 0.51 0.3 0.17 0.26 0.31
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.06 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.05 0 0.09 0 0.16 0.18 0.13 0.47 0.56 0.61 0.46 0.11 0 0 0 0 0 0.13 0.16 0.25 0.12 0.09 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0 0.09 0.17 0.11 0.08 0.11 0.07 0 0.05 0 0.09 0 0 0 0.08 0.06 0 0 0 0.05 0.06 0.09 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 18 11 4 12 3 6 1 2 6 2 5 4 1 0 5 2 8 39 47 16 6 16 20
Bud 8 16 3 8 6 8 15 6 6 4 16 20 4 7 11 3 1 5 30 21 13 19 56 45
Bud Neck 2 8 17 6 14 7 13 4 3 1 5 7 1 0 0 7 8 4 21 32 18 11 21 8
Bud Site 8 9 5 7 5 7 7 1 2 4 3 4 0 0 0 1 0 0 – – – – – –
Cell Periphery 2 3 3 0 1 0 0 0 0 0 0 1 0 2 1 10 4 6 3 7 1 0 1 4
Cytoplasm 303 392 131 70 159 135 159 104 79 60 91 87 126 140 166 265 153 210 217 266 55 12 57 58
Endoplasmic Reticulum 4 7 2 0 1 2 1 0 3 4 1 0 1 1 1 25 8 16 4 4 2 0 2 1
Endosome 4 0 0 1 0 1 0 0 0 0 1 1 0 5 13 10 5 8 10 14 10 2 5 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 2 1 3 0 1 1 2 0
Mitochondria 23 22 1 9 3 31 40 16 58 65 115 78 16 5 7 7 4 7 52 82 46 9 18 20
Nucleus 2 4 0 3 2 1 5 1 1 3 0 1 0 0 1 2 2 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 1 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1
Peroxisomes 7 0 1 3 1 1 2 1 0 0 0 1 1 6 8 4 4 2 2 2 1 3 5 4
SpindlePole 17 9 14 18 22 15 26 9 2 6 4 16 0 4 4 26 11 10 12 17 8 4 20 15
Vac/Vac Membrane 5 1 2 1 0 3 3 0 1 0 0 1 1 0 3 10 3 5 11 15 4 1 7 3
Unique Cell Count 360 454 162 105 195 194 228 121 124 116 188 170 144 162 197 325 181 254 420 526 186 77 219 191
Labelled Cell Count 393 490 191 130 227 214 278 143 157 153 239 222 154 171 215 378 207 285 420 526 186 77 219 191


Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.0 4.9 4.4 5.0 3.8 4.0 4.2 3.4 3.2 3.1 3.6 4.1 4.3 4.5 6.6 7.3 6.1 4.4 4.4 4.6
Std Deviation (1e-4) 0.6 0.5 1.4 1.4 1.6 1.1 1.2 1.3 1.2 0.6 0.5 0.8 1.1 0.9 1.4 2.1 11.9 1.9 1.6 1.8 1.7
Intensity Change (Log2) – – – -0.14 0.01 -0.37 -0.29 -0.22 -0.52 -0.61 -0.67 -0.46 -0.24 -0.2 -0.13 0.43 0.58 0.32 -0.16 -0.17 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0312 -0.2261 -0.1362 -0.8995 -0.0229 -0.2338 1.2405 0.4584 0.7694 0.388 0.3334 0.6207 -0.1899 -0.3182 -0.0446 0.0947 -0.13 -0.1718
Actin 0.0758 0.0158 0.0495 0.0042 0.0702 0.0283 0.0915 0.0291 0.0248 0.0098 0.0234 0.0496 0.0631 0.0096 0.0371 0.0077 0.0826 0.0296
Bud 0.004 0.0039 0.025 0.0003 0.0153 0.0028 0.0109 0.009 0.0241 0.0344 0.0048 0.0034 0.0115 0.0097 0.0194 0.0169 0.0141 0.0008
Bud Neck 0.0273 0.01 0.0156 0.0016 0.0047 0.1982 0.0243 0.0119 0.0041 0.0064 0.0877 0.2831 0.0309 0.0057 0.0024 0.0013 0.0045 0.171
Bud Periphery 0.0139 0.0063 0.055 0.0011 0.0483 0.0052 0.029 0.0155 0.0575 0.0627 0.0064 0.0103 0.028 0.0132 0.0488 0.061 0.0403 0.0014
Bud Site 0.0617 0.1196 0.0429 0.0009 0.0085 0.0051 0.0473 0.1547 0.0481 0.0091 0.0077 0.0044 0.0567 0.1354 0.0236 0.0052 0.0058 0.0024
Cell Periphery 0.0018 0.003 0.0018 0.0021 0.002 0.001 0.0017 0.0033 0.0023 0.0008 0.0004 0.0015 0.0027 0.0014 0.0013 0.0012 0.0011 0.0005
Cytoplasm 0.1411 0.1965 0.1897 0.1901 0.3642 0.1959 0.128 0.2353 0.2187 0.266 0.2588 0.142 0.1574 0.1832 0.1918 0.1405 0.2134 0.2785
Cytoplasmic Foci 0.1672 0.0862 0.0755 0.037 0.0957 0.1364 0.1341 0.0994 0.0597 0.07 0.0985 0.1714 0.1505 0.0609 0.0623 0.0388 0.1209 0.1186
Eisosomes 0.0012 0.0009 0.0007 0.001 0.0034 0.0004 0.0014 0.0009 0.0006 0.0001 0.0002 0.0005 0.0011 0.0005 0.0007 0.0002 0.0006 0.0003
Endoplasmic Reticulum 0.0081 0.0036 0.0026 0.008 0.007 0.0023 0.0078 0.0032 0.0046 0.0017 0.0041 0.0025 0.0063 0.0029 0.0032 0.0029 0.0043 0.0027
Endosome 0.0444 0.0148 0.0252 0.0283 0.0696 0.0162 0.0641 0.0245 0.0287 0.031 0.0386 0.0192 0.0424 0.0118 0.0229 0.0594 0.0454 0.0242
Golgi 0.0186 0.0036 0.0106 0.0014 0.018 0.0102 0.0245 0.005 0.0074 0.0097 0.0263 0.0101 0.0181 0.0033 0.0086 0.0133 0.0137 0.0096
Lipid Particles 0.0359 0.0058 0.0114 0.0081 0.0064 0.006 0.0214 0.0037 0.0033 0.0006 0.0137 0.0081 0.012 0.003 0.0039 0.0013 0.0193 0.0044
Mitochondria 0.0103 0.003 0.0085 0.0016 0.0092 0.0112 0.0237 0.0049 0.013 0.0157 0.0126 0.0093 0.0134 0.0027 0.0161 0.0387 0.0202 0.0066
None 0.3212 0.5014 0.4515 0.7015 0.2274 0.3478 0.3245 0.3763 0.4789 0.4472 0.3642 0.216 0.3363 0.5364 0.5252 0.5691 0.3535 0.3
Nuclear Periphery 0.0127 0.0008 0.0007 0.0018 0.0087 0.005 0.0123 0.0007 0.0016 0.0009 0.0027 0.0008 0.0047 0.0009 0.0029 0.0017 0.0044 0.0023
Nucleolus 0.0022 0.0008 0.0012 0.0003 0.001 0.0003 0.0012 0.001 0.001 0.0005 0.0007 0.0007 0.0053 0.0007 0.0006 0.0007 0.0007 0.0004
Nucleus 0.0032 0.0014 0.0017 0.0007 0.0036 0.0007 0.0028 0.0017 0.0024 0.002 0.003 0.0011 0.0043 0.0024 0.0028 0.0023 0.0014 0.0012
Peroxisomes 0.0308 0.0144 0.022 0.001 0.0167 0.0222 0.032 0.0125 0.0093 0.0147 0.0361 0.0593 0.0395 0.0096 0.0181 0.0017 0.0291 0.0389
Punctate Nuclear 0.0094 0.0024 0.0038 0.0008 0.0035 0.0033 0.0078 0.0027 0.0025 0.0014 0.0059 0.005 0.0104 0.0039 0.0036 0.0175 0.0192 0.0044
Vacuole 0.0076 0.005 0.0043 0.0071 0.0117 0.001 0.0071 0.0041 0.0055 0.0115 0.0033 0.0013 0.0045 0.0025 0.0035 0.0143 0.0039 0.0016
Vacuole Periphery 0.0016 0.0007 0.0009 0.0009 0.0048 0.0007 0.0027 0.0006 0.0021 0.0038 0.001 0.0004 0.0011 0.0003 0.0009 0.0045 0.0017 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.6153 10.0395 8.6913 7.7775 10.314 4.2091 10.6796 9.8485 7.9721 9.6527
Translational Efficiency 0.7906 0.5971 0.4534 0.4647 0.3519 0.8159 0.4836 0.3246 0.5804 0.4864

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1799 1533 483 1491 1652 1878 667 26 3451 3411 1150 1517

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 625.95 694.23 884.37 791.28 660.04 688.22 822.74 1208.50 642.27 690.92 848.62 798.43
Standard Deviation 66.07 83.22 111.28 103.97 72.90 80.14 101.36 134.56 71.48 81.59 109.93 117.76
Intensity Change Log 2 — 0.149366 0.498603 0.338141 — 0.060316 0.317883 0.872592 — 0.104348 0.408677 0.636962

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000441 0.001134 0.004618 0.005607 0.001146 0.000723 0.004518 0.000550 0.000778 0.000908 0.004560 0.005520
Bud Neck 0.060626 0.057214 0.028926 0.062506 0.049310 0.062818 0.063111 0.024856 0.055209 0.060299 0.048753 0.061861
Bud Site 0.019007 0.038395 0.078134 0.176718* 0.020065 0.040925 0.137785* 0.128860* 0.019514 0.039788 0.112732* 0.175897*
Cell Periphery 0.000262 0.000208 0.000484 0.000427 0.000209 0.000189 0.000577 0.000067 0.000236 0.000198 0.000538 0.000420
Cytoplasm 0.446032* 0.354245* 0.237683* 0.360017* 0.389792* 0.338990* 0.291765* 0.674100* 0.419109* 0.345846* 0.269050* 0.365400*
Cytoplasmic Foci 0.242068* 0.280404* 0.049958 0.046243 0.295183* 0.274516* 0.064300 0.006212 0.267494* 0.277162* 0.058276 0.045557
Eisosomes 0.000464 0.000252 0.000158 0.000164 0.000433 0.000231 0.000225 0.000021 0.000449 0.000240 0.000197 0.000161
Endoplasmic Reticulum 0.001216 0.000903 0.012108 0.002660 0.001483 0.000584 0.003245 0.000159 0.001344 0.000727 0.006967 0.002617
Endosome 0.013186 0.030092 0.012083 0.007572 0.013911 0.031104 0.010181 0.000353 0.013533 0.030649 0.010980 0.007448
Golgi 0.004531 0.011654 0.000626 0.001225 0.007949 0.012155 0.001162 0.000282 0.006167 0.011930 0.000937 0.001208
Lipid Particles 0.010398 0.007740 0.007706 0.001972 0.013153 0.008994 0.004356 0.000229 0.011717 0.008430 0.005763 0.001942
Mitochondria 0.008080 0.021854 0.001266 0.003039 0.015401 0.030304 0.001364 0.003255 0.011584 0.026507 0.001323 0.003042
Mitotic Spindle 0.000429 0.009727 0.017650 0.029660 0.002401 0.009156 0.011308 0.003281 0.001373 0.009413 0.013972 0.029208
None 0.003334 0.001865 0.004200 0.002279 0.001316 0.001693 0.004652 0.003964 0.002368 0.001771 0.004462 0.002308
Nuclear Periphery 0.000214 0.000344 0.002969 0.000704 0.000312 0.000382 0.000936 0.000248 0.000261 0.000365 0.001790 0.000696
Nuclear Periphery Foci 0.000197 0.000389 0.004633 0.000857 0.000154 0.000252 0.001510 0.000030 0.000177 0.000313 0.002821 0.000843
Nucleolus 0.000878 0.001501 0.002161 0.000652 0.000754 0.001514 0.001395 0.000227 0.000818 0.001508 0.001717 0.000645
Nucleus 0.123728 0.077713 0.368927* 0.137428* 0.122772 0.079725 0.233755* 0.096968 0.123270 0.078821 0.290527* 0.136734*
Peroxisomes 0.012502 0.029867 0.015443 0.017216 0.018430 0.032238 0.040583 0.001773 0.015340 0.031172 0.030024 0.016951
Vacuole 0.050739 0.071086 0.149548 0.140592 0.044534 0.070259 0.122776 0.054432 0.047769 0.070631 0.134020 0.139116
Vacuole Periphery 0.001669 0.003414 0.000719 0.002463 0.001295 0.003248 0.000497 0.000132 0.001490 0.003322 0.000591 0.002423

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.31 -4.01 -8.05 -6.44 0.02 1.30 -4.60 2.13 2.01 6.42 -0.71 -6.29 -7.06 -7.05 -0.19
Bud Neck 0.86 7.55 2.99 2.18 -5.07 -3.23 -0.44 2.53 3.64 2.60 -1.51 3.90 2.29 3.62 -1.56
Bud Site -6.48 -5.10 -17.74 -14.02 -7.89 -6.93 -10.83 -1.16 -0.31 2.56 -9.53 -11.81 -17.92 -14.31 -4.78
Cell Periphery 1.95 -3.63 -3.14 -5.20 1.61 1.24 -7.73 10.39 12.17 12.16 2.47 -8.01 -4.61 -6.56 3.81
Cytoplasm 9.97 21.78 10.55 0.88 -12.20 5.64 9.72 -1.08 -2.00 -3.20 11.38 20.88 8.37 -0.18 -10.39
Cytoplasmic Foci -5.02 32.38 38.92 39.33 -0.54 2.69 34.74 39.97 38.76 8.34 -1.74 46.72 54.55 54.82 2.99
Eisosomes 5.46 8.44 8.91 4.14 1.15 7.30 6.93 14.78 14.78 12.85 8.79 10.54 12.75 5.89 4.15
Endoplasmic Reticulum 1.15 -14.40 -4.75 -6.20 12.20 2.13 -5.54 2.13 0.32 10.27 2.53 -14.55 -4.32 -7.15 10.58
Endosome -8.69 1.69 6.80 13.21 4.73 -9.57 3.64 14.25 19.98 9.00 -13.01 3.92 8.89 19.35 4.54
Golgi -7.96 11.37 10.20 13.13 -2.52 -3.82 11.70 13.59 12.88 3.00 -8.05 15.53 15.60 17.45 -0.57
Lipid Particles 2.84 2.95 11.36 9.69 8.09 4.04 8.80 12.34 10.86 6.22 4.74 8.70 16.72 14.80 10.10
Mitochondria -6.60 7.66 5.92 10.24 -2.51 -5.88 9.78 8.60 13.29 -0.41 -9.04 12.33 10.06 16.60 -2.51
Mitotic Spindle -5.20 -5.13 -8.13 -4.05 -1.57 -4.12 -3.34 -0.02 3.73 3.19 -6.79 -6.09 -7.72 -4.53 -2.92
None 2.49 -0.20 2.03 -0.42 2.21 -1.85 -3.64 -1.55 -1.25 0.57 1.88 -2.65 0.62 -0.82 2.90
Nuclear Periphery -2.66 -14.63 -17.57 -10.65 10.92 -0.79 -9.53 -3.17 -2.93 2.77 -2.09 -17.12 -13.57 -12.06 10.24
Nuclear Periphery Foci -3.35 -8.68 -10.28 -6.80 7.17 -3.44 -7.87 -0.07 1.67 7.69 -4.48 -11.19 -10.76 -8.60 7.88
Nucleolus -3.66 -4.15 2.42 4.93 4.93 -4.36 -2.43 2.61 6.50 3.40 -5.69 -4.20 1.99 6.59 4.89
Nucleus 9.77 -20.63 -6.69 -13.89 17.01 9.11 -12.30 -1.60 -2.85 2.20 13.40 -22.37 -7.27 -15.19 15.80
Peroxisomes -7.79 0.06 -0.30 7.15 -0.24 -5.80 -3.15 11.04 14.61 7.58 -9.69 -3.02 1.64 9.64 3.73
Vacuole -6.44 -15.19 -24.22 -20.21 -2.01 -8.75 -15.42 -4.04 -3.58 -1.82 -10.62 -21.41 -25.50 -21.36 -3.67
Vacuole Periphery -3.37 3.06 0.04 2.61 -1.85 -6.28 9.13 15.65 10.51 5.12 -6.07 5.35 -0.22 2.85 -2.06
Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress
Localization
Cell Percentages cytoplasm (14%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rgd3

Rgd3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rgd3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available