Standard name | |
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Human Ortholog | |
Description | Farnesyl-diphosphate farnesyl transferase (squalene synthase); joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 0 | 1 | 2 | 5 | 4 | 1 | 1 | 6 | 7 | 1 | 3 | 4 |
Bud | 2 | 3 | 3 | 2 | 5 | 13 | 21 | 24 | 15 | 26 | 21 | 16 | 0 | 1 | 1 | 2 | 2 | 1 | 0 | 1 | 0 | 1 | 2 | 2 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 2 | 3 | 1 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 2 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 4 | 8 | 11 | 17 | 18 | 20 | 17 | 21 | 13 | 15 | 35 | 2 | 14 | 13 | 16 | 9 | 9 | 0 | 3 | 2 | 1 | 4 | 10 |
Cytoplasm | 66 | 211 | 199 | 158 | 236 | 187 | 262 | 347 | 127 | 132 | 140 | 192 | 11 | 130 | 125 | 104 | 100 | 60 | 41 | 137 | 40 | 15 | 36 | 58 |
Endoplasmic Reticulum | 17 | 16 | 7 | 21 | 21 | 42 | 30 | 14 | 32 | 41 | 47 | 52 | 17 | 46 | 44 | 5 | 4 | 0 | 48 | 53 | 228 | 66 | 106 | 112 |
Endosome | 0 | 1 | 12 | 8 | 20 | 12 | 24 | 20 | 3 | 3 | 4 | 9 | 0 | 2 | 0 | 1 | 0 | 1 | 3 | 3 | 2 | 0 | 1 | 1 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 0 |
Mitochondria | 6 | 16 | 44 | 82 | 112 | 219 | 309 | 281 | 178 | 203 | 173 | 238 | 0 | 0 | 0 | 9 | 8 | 1 | 9 | 32 | 26 | 23 | 37 | 37 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 4 | 6 | 17 | 5 | 6 | 4 | 4 | 0 | 1 | 1 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 15 | 9 | 12 | 7 | 8 | 4 | 11 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 4 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 5 | 25 | 29 | 115 | 97 | 142 | 100 | 46 | 47 | 42 | 91 | 0 | 6 | 6 | 12 | 10 | 5 | 3 | 11 | 4 | 5 | 15 | 22 |
Unique Cell Count | 87 | 245 | 269 | 260 | 416 | 477 | 654 | 676 | 328 | 378 | 347 | 514 | 28 | 182 | 177 | 145 | 128 | 74 | 114 | 261 | 323 | 122 | 214 | 261 |
Labelled Cell Count | 92 | 258 | 298 | 313 | 530 | 612 | 830 | 845 | 438 | 489 | 452 | 654 | 30 | 203 | 192 | 159 | 142 | 81 | 114 | 261 | 323 | 122 | 214 | 261 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 4.4 | 5.1 | 5.0 | 5.5 | 4.9 | 5.0 | 5.4 | 5.1 | 5.1 | 5.0 | 5.0 | 6.3 | 6.2 | 6.5 | 6.5 | 6.2 | 6.7 | 5.5 | 5.6 | 6.1 |
Std Deviation (1e-4) | 0.6 | 1.1 | 1.5 | 1.2 | 1.7 | 1.1 | 1.3 | 1.3 | 1.6 | 1.5 | 1.1 | 1.3 | 1.0 | 0.9 | 0.9 | 1.1 | 1.1 | 1.3 | 0.8 | 0.9 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.09 | -0.07 | -0.03 | 0.09 | -0.02 | -0.02 | -0.04 | -0.03 | 0.3 | 0.28 | 0.35 | 0.34 | 0.29 | 0.38 | 0.11 | 0.13 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | -0.3 | -0.5 | -0.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.136 | 2.2844 | 2.0741 | 1.8482 | 1.8526 | 2.2456 | 3.804 | 4.8278 | 4.1516 | 3.6501 | 4.1234 | 4.266 | 2.7501 | 3.1903 | 2.915 | 2.8351 | 2.8333 | 2.4599 |
Actin | 0.0008 | 0.0008 | 0.0036 | 0.0004 | 0.0004 | 0.003 | 0.0048 | 0.0109 | 0.0035 | 0.0318 | 0.0131 | 0.0009 | 0.0129 | 0.0002 | 0.0067 | 0 | 0.0048 | 0.0009 |
Bud | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0005 | 0.0008 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0029 | 0.0005 | 0.0023 | 0.0003 | 0.0032 | 0.0026 | 0.0009 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0008 |
Bud Periphery | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0.0002 | 0.0003 | 0.0003 | 0.0006 | 0.001 | 0.0009 | 0.0005 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0002 | 0.0008 | 0.0038 | 0 | 0.0001 | 0.0002 | 0.004 | 0.005 | 0.005 | 0.0026 | 0.0007 | 0.0003 | 0.0049 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0001 |
Cell Periphery | 0.0009 | 0.0059 | 0.0012 | 0.0004 | 0.0003 | 0.0005 | 0.0012 | 0.0039 | 0.0012 | 0.0025 | 0.0007 | 0.0004 | 0.0006 | 0.0005 | 0.0005 | 0.0003 | 0.0012 | 0.0012 |
Cytoplasm | 0.1188 | 0.1592 | 0.1504 | 0.0622 | 0.203 | 0.1552 | 0.113 | 0.1657 | 0.0983 | 0.1074 | 0.1459 | 0.1376 | 0.109 | 0.1239 | 0.0602 | 0.009 | 0.1566 | 0.0962 |
Cytoplasmic Foci | 0.0068 | 0.0032 | 0.0072 | 0.0013 | 0.0041 | 0.0051 | 0.0098 | 0.0067 | 0.0107 | 0.0262 | 0.0142 | 0.0074 | 0.0136 | 0.0015 | 0.0053 | 0.0001 | 0.0082 | 0.0035 |
Eisosomes | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0007 | 0 | 0 | 0 | 0.0025 | 0 |
Endoplasmic Reticulum | 0.8074 | 0.7332 | 0.7229 | 0.8759 | 0.721 | 0.7584 | 0.7612 | 0.725 | 0.7995 | 0.626 | 0.6912 | 0.789 | 0.7445 | 0.8585 | 0.891 | 0.9813 | 0.6016 | 0.8314 |
Endosome | 0.0152 | 0.0227 | 0.0258 | 0.0132 | 0.0156 | 0.0171 | 0.0228 | 0.0198 | 0.0229 | 0.0526 | 0.0625 | 0.0156 | 0.012 | 0.0038 | 0.0026 | 0.0009 | 0.0107 | 0.0095 |
Golgi | 0.0018 | 0.0018 | 0.0031 | 0.0004 | 0.0006 | 0.0015 | 0.0048 | 0.0034 | 0.0038 | 0.0184 | 0.0154 | 0.002 | 0.0051 | 0.0004 | 0.0043 | 0.0001 | 0.0053 | 0.0012 |
Lipid Particles | 0.009 | 0.0061 | 0.0069 | 0.0021 | 0.0028 | 0.0038 | 0.0159 | 0.0065 | 0.0101 | 0.0297 | 0.0114 | 0.0063 | 0.0103 | 0.0025 | 0.0053 | 0.0003 | 0.0087 | 0.0225 |
Mitochondria | 0.0003 | 0.0021 | 0.0009 | 0.0006 | 0.0004 | 0.0007 | 0.0023 | 0.0014 | 0.0013 | 0.0115 | 0.0057 | 0.0013 | 0.0016 | 0 | 0.0005 | 0 | 0.114 | 0.0001 |
None | 0.013 | 0.0314 | 0.0478 | 0.0046 | 0.0211 | 0.0214 | 0.02 | 0.0147 | 0.0193 | 0.0171 | 0.0055 | 0.0146 | 0.0494 | 0.0007 | 0.0008 | 0.0001 | 0.0658 | 0.0187 |
Nuclear Periphery | 0.0168 | 0.0183 | 0.0161 | 0.0297 | 0.0147 | 0.0202 | 0.0272 | 0.0154 | 0.0107 | 0.0448 | 0.0147 | 0.0118 | 0.0169 | 0.0047 | 0.0071 | 0.0053 | 0.0118 | 0.0076 |
Nucleolus | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0015 | 0.0024 | 0.0021 | 0.0035 | 0.0097 | 0.003 | 0.0028 | 0.0037 | 0.0025 | 0.0032 | 0.0023 | 0.0034 | 0.0017 | 0.0005 | 0.0004 | 0.0004 | 0.0017 | 0.0013 |
Peroxisomes | 0.0012 | 0 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0014 | 0.0026 | 0.0009 | 0.0097 | 0.0019 | 0.0004 | 0.0069 | 0 | 0.0128 | 0 | 0.0031 | 0.0001 |
Punctate Nuclear | 0.0011 | 0.0005 | 0.0008 | 0.0004 | 0.0011 | 0.0016 | 0.002 | 0.0016 | 0.0025 | 0.0015 | 0.0044 | 0.0026 | 0.0053 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0004 |
Vacuole | 0.0043 | 0.0095 | 0.0051 | 0.004 | 0.0042 | 0.004 | 0.0038 | 0.0093 | 0.0051 | 0.0068 | 0.0043 | 0.0037 | 0.0028 | 0.0018 | 0.0016 | 0.002 | 0.0022 | 0.0038 |
Vacuole Periphery | 0.0006 | 0.0013 | 0.001 | 0.0012 | 0.0005 | 0.0008 | 0.002 | 0.001 | 0.0008 | 0.0026 | 0.0017 | 0.0005 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 154.5593 | 139.6443 | 130.4871 | 149.9219 | 127.5227 | 155.2998 | 128.6809 | 139.6674 | 144.7577 | 132.6607 |
Translational Efficiency | 1.3094 | 1.2351 | 1.4664 | 1.3854 | 1.5038 | 1.4396 | 1.4614 | 1.2725 | 1.4858 | 1.4741 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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