Standard name
Human Ortholog
Description Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.09 0.07 0.09 0.08 0.08 – 0.06 0.09 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0.05 0.05 0.08 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Cytoplasm 0.51 0.67 0.84 0.7 0.68 0.47 0.42 – 0.41 0.32 0.26 0.22 0.92 0.96 0.94 0.6 0.64 0.68 0.23 0.21 0.2 0.44 0.31 0.42
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.11 0.08 0.12 0 0 0 0 0 0
Endosome 0 0.15 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.07 0.05 0.1 0.09 0.06 0 0 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.53 0.06 0 0.13 0.06 0.3 0.34 – 0.47 0.45 0.67 0.54 0 0 0 0.08 0.05 0.06 0.39 0.34 0.45 0.24 0.34 0.22
Nucleus 0 0 0 0 0 0.06 0.11 – 0.12 0.11 0.12 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
SpindlePole 0 0.06 0 0.09 0.13 0.18 0.24 – 0.14 0.23 0.13 0.23 0 0 0 0.08 0.09 0.11 0.08 0.09 0.06 0 0.06 0.08
Vac/Vac Membrane 0.08 0.09 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0.11 0.11 0.06 0.08 0.09 0.06 0.09 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 2 – 0 1 0 0 0 0 0 7 4 1 0 9 3 1 3 3
Bud 1 4 0 3 1 4 6 – 6 10 10 4 0 0 0 3 2 2 3 9 1 1 3 5
Bud Neck 0 2 9 8 17 15 17 – 11 18 8 25 0 0 0 1 3 3 2 12 6 3 4 4
Bud Site 0 0 0 0 0 1 3 – 9 10 18 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 6 0 0 0 1 2 – 1 1 0 0 2 0 1 14 11 8 0 0 1 0 0 0
Cytoplasm 50 187 86 80 124 88 95 – 77 65 56 44 66 183 222 126 123 172 26 83 34 34 36 78
Endoplasmic Reticulum 1 4 1 0 1 2 0 – 0 2 0 2 0 1 0 23 16 30 0 0 1 0 1 1
Endosome 3 41 0 1 5 0 0 – 0 0 0 0 1 4 4 15 13 12 11 37 10 3 4 8
Golgi 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 2 1 4 1 0 0 0 0 1
Mitochondria 52 17 2 15 11 56 77 – 88 90 144 107 1 0 4 17 10 16 46 138 76 18 40 41
Nucleus 1 4 3 5 8 12 24 – 22 22 26 28 3 2 0 1 1 0 1 7 1 1 1 1
Nuclear Periphery 0 0 0 0 0 0 2 – 0 0 1 1 0 0 1 1 0 0 0 4 0 0 0 1
Nucleolus 1 0 1 1 1 0 1 – 0 0 2 1 0 0 0 0 0 0 1 6 1 0 2 1
Peroxisomes 0 1 0 0 0 1 0 – 1 0 2 1 0 1 3 13 3 4 0 1 2 0 0 0
SpindlePole 3 16 4 10 23 34 54 – 26 47 27 45 1 1 4 16 17 27 8 35 9 3 6 14
Vac/Vac Membrane 8 26 3 1 11 4 1 – 2 2 0 3 2 1 6 23 21 15 8 36 10 6 5 16
Unique Cell Count 99 281 102 115 182 189 226 188 202 214 200 72 190 236 210 193 254 118 401 170 79 119 187
Labelled Cell Count 120 308 110 125 202 218 284 243 268 294 270 76 193 245 262 225 294 118 401 170 79 119 187


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.4 5.0 4.9 5.0 4.2 4.2 – 3.5 3.8 3.3 3.4 5.2 5.4 5.1 6.3 6.5 5.6 5.0 5.1 4.9
Std Deviation (1e-4) 0.6 0.7 0.9 1.5 1.5 1.5 1.3 – 1.2 1.2 1.6 0.8 1.2 1.5 1.7 1.3 1.6 1.5 2.0 1.9 1.3
Intensity Change (Log2) – – – -0.03 -0.0 -0.24 -0.24 – -0.5 -0.39 -0.57 -0.56 0.06 0.11 0.03 0.34 0.39 0.18 0.02 0.04 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.4 2.4 1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3161 -0.3122 -0.1241 -0.6048 0.7348 -0.2718 0.4107 -0.0642 -0.0748 0.2499 -0.1111 -0.1152 -0.2223 -0.3217 -0.3899 0.411 -0.1299 -0.3032
Actin 0.0221 0.0023 0.007 0.0005 0.0068 0.0097 0.069 0.0016 0.017 0.0391 0.0076 0.001 0.0034 0.0031 0.0053 0.005 0.0099 0.0019
Bud 0.0027 0.0043 0.0072 0.0015 0.0011 0.004 0.0016 0.0019 0.0011 0.0041 0.0003 0.0032 0.0017 0.0221 0.0019 0.0018 0.0058 0.0142
Bud Neck 0.0047 0.0009 0.0098 0.0055 0.0093 0.0113 0.0155 0.0006 0.0041 0.008 0.0006 0.0246 0.0104 0.0089 0.0021 0.0176 0.0147 0.0024
Bud Periphery 0.0065 0.0062 0.0071 0.0052 0.0013 0.0078 0.0024 0.0047 0.0023 0.0093 0.0004 0.0045 0.0027 0.0162 0.0022 0.002 0.0024 0.0094
Bud Site 0.0236 0.027 0.0193 0.0041 0.0152 0.003 0.015 0.0136 0.0269 0.0232 0.0007 0.0024 0.0145 0.0383 0.0146 0.0804 0.0049 0.0015
Cell Periphery 0.0019 0.0013 0.0019 0.0016 0.0004 0.0007 0.0014 0.0015 0.0016 0.002 0.0001 0.0007 0.003 0.0021 0.0008 0.0009 0.0016 0.0003
Cytoplasm 0.054 0.0928 0.0823 0.1294 0.1321 0.1188 0.0685 0.0601 0.0363 0.0621 0.0607 0.1092 0.1317 0.2 0.1822 0.2142 0.1419 0.1907
Cytoplasmic Foci 0.0856 0.0544 0.0709 0.0496 0.0467 0.108 0.0744 0.0243 0.0564 0.0293 0.0503 0.0416 0.0561 0.0599 0.0692 0.0759 0.1585 0.1167
Eisosomes 0.0011 0.0005 0.0006 0.0003 0.0003 0.0002 0.001 0.0005 0.0004 0.0005 0.0002 0.0003 0.0005 0.0004 0.0003 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0071 0.0031 0.0038 0.0056 0.005 0.0051 0.0102 0.0011 0.0023 0.0016 0.0004 0.0013 0.013 0.0048 0.0028 0.0039 0.0055 0.0041
Endosome 0.0673 0.0347 0.0465 0.0571 0.1791 0.0777 0.0525 0.011 0.0395 0.019 0.0075 0.0234 0.0579 0.0358 0.0521 0.1368 0.1291 0.0853
Golgi 0.011 0.0032 0.0133 0.0053 0.0479 0.0071 0.0093 0.0016 0.0078 0.0141 0.0052 0.0034 0.0049 0.0028 0.0098 0.0259 0.0171 0.0083
Lipid Particles 0.0285 0.0066 0.021 0.0069 0.0104 0.0163 0.03 0.0025 0.0202 0.0262 0.0192 0.0131 0.0263 0.0066 0.0161 0.0221 0.024 0.0115
Mitochondria 0.0137 0.0084 0.0226 0.0454 0.0212 0.0297 0.0185 0.0043 0.0341 0.0144 0.0122 0.0252 0.0118 0.0113 0.0436 0.0104 0.0037 0.0162
None 0.5632 0.7212 0.6205 0.634 0.44 0.5276 0.5285 0.8444 0.6548 0.6718 0.7606 0.7184 0.5753 0.5508 0.5662 0.3178 0.4033 0.4854
Nuclear Periphery 0.0086 0.0024 0.0055 0.0017 0.0057 0.0059 0.0102 0.0004 0.0083 0.0015 0.0002 0.002 0.0174 0.0018 0.0012 0.0028 0.002 0.0023
Nucleolus 0.0072 0.0021 0.006 0.0149 0.0045 0.0047 0.0063 0.0023 0.0152 0.0187 0.0002 0.0016 0.0139 0.0015 0.0047 0.0031 0.002 0.0018
Nucleus 0.0084 0.0033 0.0082 0.0062 0.0199 0.012 0.0216 0.0066 0.0128 0.0082 0.0003 0.0019 0.0057 0.0034 0.0022 0.0069 0.0036 0.0058
Peroxisomes 0.0152 0.0038 0.0153 0.0053 0.0026 0.0199 0.0178 0.0061 0.0279 0.0124 0.072 0.007 0.0087 0.0041 0.0088 0.0047 0.0439 0.0093
Punctate Nuclear 0.0105 0.0044 0.0084 0.0019 0.0045 0.0122 0.0119 0.0006 0.0067 0.0197 0.0008 0.0076 0.0098 0.0026 0.0025 0.0097 0.0104 0.0048
Vacuole 0.051 0.0147 0.0184 0.0148 0.0407 0.015 0.0292 0.0066 0.0207 0.0128 0.0005 0.0062 0.027 0.0212 0.0093 0.0505 0.0145 0.0249
Vacuole Periphery 0.006 0.0024 0.0043 0.0031 0.0053 0.0032 0.0053 0.0036 0.0037 0.002 0.0002 0.0016 0.0043 0.0024 0.0019 0.0075 0.0013 0.0028

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2926 6.8026 10.9369 10.4259 5.3442 22.8164 9.3141 13.7333 14.5393 11.0582
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
549 201 979 165 900 987 1607 120 1449 1188 2586 285

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 588.21 723.61 775.78 828.58 642.59 684.77 759.26 795.85 621.99 691.34 765.51 814.80
Standard Deviation 55.08 91.19 101.75 135.49 77.14 99.40 97.62 90.51 74.44 99.13 99.53 119.74
Intensity Change Log 2 — 0.298881 0.399316 0.494310 — 0.091721 0.240695 0.308598 — 0.194440 0.318680 0.400337

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000266 0.000869 0.000529 0.006065 0.000291 0.000697 0.000160 0.000527 0.000281 0.000726 0.000300 0.003733
Bud Neck 0.073821 0.087218 0.054192 0.020589 0.044252 0.081455 0.030064 0.059318 0.055455 0.082430 0.039198 0.036896
Bud Site 0.010584 0.046022 0.021530 0.086549 0.008176 0.033058 0.016056 0.037146 0.009088 0.035251 0.018128 0.065748
Cell Periphery 0.000419 0.000146 0.000237 0.000275 0.000409 0.000268 0.000205 0.000188 0.000413 0.000248 0.000217 0.000238
Cytoplasm 0.254030* 0.207761* 0.163259* 0.287227* 0.205828* 0.156597* 0.353581* 0.276413* 0.224091* 0.165253* 0.281529* 0.282673*
Cytoplasmic Foci 0.365356* 0.232512* 0.022947 0.040531 0.348006* 0.229343* 0.026625 0.036046 0.354579* 0.229879* 0.025232 0.038643
Eisosomes 0.000586 0.000148 0.000086 0.000112 0.000339 0.000196 0.000056 0.000142 0.000432 0.000188 0.000068 0.000124
Endoplasmic Reticulum 0.001945 0.000962 0.005578 0.004249 0.001482 0.000857 0.001976 0.003734 0.001657 0.000875 0.003340 0.004032
Endosome 0.033992 0.067382 0.011045 0.014552 0.054593 0.100632 0.006572 0.009404 0.046788 0.095007 0.008265 0.012385
Golgi 0.012624 0.009233 0.000450 0.003591 0.013216 0.022887 0.000194 0.000728 0.012992 0.020577 0.000291 0.002385
Lipid Particles 0.045890 0.004827 0.004207 0.002562 0.035980 0.012522 0.002386 0.006235 0.039735 0.011220 0.003076 0.004109
Mitochondria 0.051426 0.018321 0.001757 0.005858 0.060225 0.076298 0.001058 0.000757 0.056891 0.066489 0.001322 0.003710
Mitotic Spindle 0.000730 0.015142 0.005813 0.020876 0.003719 0.025963 0.007522 0.023852 0.002587 0.024132 0.006875 0.022129
None 0.005900 0.003301 0.007029 0.001788 0.002758 0.003128 0.006703 0.006564 0.003949 0.003158 0.006826 0.003799
Nuclear Periphery 0.000441 0.000667 0.003365 0.001848 0.001301 0.000727 0.002161 0.001823 0.000975 0.000717 0.002617 0.001837
Nuclear Periphery Foci 0.000288 0.000585 0.001629 0.002364 0.000444 0.000442 0.000682 0.001269 0.000385 0.000466 0.001040 0.001903
Nucleolus 0.003418 0.004714 0.014060 0.000679 0.004058 0.007463 0.003776 0.005067 0.003816 0.006998 0.007669 0.002527
Nucleus 0.073874 0.124281 0.601614* 0.215846* 0.098573 0.077283 0.445849* 0.367651* 0.089215 0.085234 0.504818* 0.279764*
Peroxisomes 0.033903 0.066751 0.004166 0.003321 0.043963 0.059785 0.001701 0.004792 0.040152 0.060963 0.002634 0.003941
Vacuole 0.027644 0.106061 0.074981 0.269110* 0.067132 0.101259 0.091928 0.157172 0.052171 0.102072 0.085512 0.221978*
Vacuole Periphery 0.002864 0.003098 0.001526 0.012008 0.005256 0.009139 0.000747 0.001173 0.004350 0.008117 0.001042 0.007446

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.26 -5.27 -1.47 -1.16 -1.26 -2.93 1.29 -2.02 -0.45 -2.40 -3.80 -1.95 -1.67 -1.26 -1.58
Bud Neck -0.54 3.03 7.81 5.82 6.30 -6.45 4.32 0.83 4.10 -0.79 -4.71 5.04 5.52 8.98 2.23
Bud Site -5.10 -3.13 -3.28 -0.26 -2.38 -9.37 -2.53 -2.50 0.92 -1.72 -10.51 -3.95 -4.11 -0.39 -3.09
Cell Periphery 4.87 3.17 2.52 -2.59 -0.37 1.17 1.85 1.56 1.09 -0.43 2.11 2.67 2.17 0.32 -0.88
Cytoplasm 2.13 7.66 0.22 -1.42 -4.12 4.67 -12.60 -1.66 -3.55 3.45 6.44 -5.62 -1.47 -4.84 1.26
Cytoplasmic Foci 7.95 34.90 31.31 12.92 -1.65 11.69 41.26 33.48 22.66 -1.38 14.74 53.96 46.35 27.95 -2.04
Eisosomes 5.93 6.68 6.15 0.81 -2.27 3.21 6.58 2.62 0.27 -2.90 6.11 9.35 6.57 1.50 -3.97
Endoplasmic Reticulum 1.05 -6.42 -3.79 -6.15 1.60 3.05 -4.71 -3.92 -4.62 -2.81 2.52 -7.80 -6.06 -8.02 -2.20
Endosome -3.48 7.67 7.56 6.80 1.58 -7.85 13.50 12.49 19.47 -0.88 -9.92 15.43 14.10 20.30 0.38
Golgi 1.74 8.32 6.80 3.17 -1.12 -4.32 10.48 10.14 12.55 -1.84 -3.97 13.37 11.36 12.18 -1.52
Lipid Particles 9.19 9.29 9.66 2.54 2.89 8.18 12.94 9.06 2.39 -2.50 11.20 15.63 14.19 4.85 -1.66
Mitochondria 4.71 9.08 8.58 2.73 -0.37 -2.13 12.16 12.22 15.00 2.28 -1.45 15.16 14.56 14.57 -0.37
Mitotic Spindle -4.05 -4.52 -2.60 -0.26 -1.39 -6.07 -3.81 -1.02 1.49 0.03 -7.14 -5.31 -2.47 1.44 -0.84
None 1.39 -0.36 2.63 1.17 4.53 -0.29 -3.62 -1.55 -1.09 0.84 0.81 -2.46 1.14 0.11 4.02
Nuclear Periphery -2.87 -13.14 -5.43 -3.61 5.06 1.91 -4.93 -2.53 -4.25 0.67 1.07 -10.45 -4.30 -5.77 3.74
Nuclear Periphery Foci -2.63 -6.56 -6.66 -4.34 0.53 -0.22 -4.52 -3.16 -3.11 -2.07 -1.99 -7.84 -6.73 -6.13 -2.00
Nucleolus -1.23 -5.81 3.05 4.62 8.21 -3.00 0.15 -0.43 1.51 -0.49 -3.31 -4.79 1.74 4.23 5.58
Nucleus -3.64 -46.34 -6.98 -3.27 17.56 2.85 -36.55 -8.39 -9.18 4.36 0.36 -55.68 -10.32 -10.25 14.02
Peroxisomes -2.99 10.29 11.34 6.81 3.13 -3.14 13.57 11.62 13.84 -1.75 -4.76 16.85 16.25 16.35 -0.24
Vacuole -7.94 -10.71 -14.34 -9.75 -12.01 -5.69 -8.16 -7.83 -6.47 -5.99 -10.27 -12.04 -15.43 -12.35 -12.63
Vacuole Periphery 0.01 4.15 -0.51 -0.51 -1.04 -4.39 10.67 9.93 10.77 -0.48 -5.19 11.35 0.94 2.97 -1.16
Standard name
Human Ortholog
Description Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34
Localization
Cell Percentages mitochondrion (19%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mdm31

Mdm31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdm31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available